Addgene is a global nonprot plasmid repository. Over 2,000 labs have deposited plasmids to Addgene and we distribute over 100,000 plasmids per year. Thus, we are in a unique position to observe and quantify how new technologies spread through the scientic community. We will compare global distribution data for Zinc Finger, TALE, and CRISPR plasmids over time. We’ll analyze CRISPR plasmids in more detail by summarizing the latest plasmid developments and by examing trends in genome engineering technologies. Finally, we’ll present data describing the research tools shared by synthetic biology labs.

1. Trends in CRISPR and
SynBio Technologies:
Data from Addgene’s
Plasmid Repository
Eric J. Perkins, Matthew Ferenc,
Julian Taylor-Parker, Larissa Haliw,
Zachary Tribbett, Elisha Fielding,
Pamela McA’Nulty, Joanne Kamens,
Melina Fan
Addgene, Cambridge, MA 02139
www.addgene.org help@addgene.org

2. Addgene: A Global, Nonprofit Plasmid
Repository
Addgene is in a unique position to observe and
quantify how new technologies spread through
the scientic community.
• Over 2,000 labs have deposited plasmids to Addgene
• Addgene distributes over 100,000 plasmids per year.
We will compare global distribution data for Zinc
Finger, TALE, and CRISPR plasmids over time.
• We’ll analyze CRISPR plasmids in more detail by
summarizing the latest plasmid developments and by
examining trends in genome engineering technologies.
Finally, we’ll present data describing the research
tools shared by synthetic biology labs.

3. Genome Engineering and Synthetic Biology
Plasmids Deposited with Addgene
The data reveal the ebb and flow of genome
engineering technology trends.

4. Genome Engineering and Syn Bio Plasmids in
the Collection
Addgene’s
collection includes
35,000 plasmids
from 2,100 labs
worldwide.
Genome
engineering and
synthetic biology
plasmids make up
approximately 10%
of the total
collection.

5. CRISPR Plasmid Requests Outpace Other
Technologies
The power and broad applicability of CRISPR technology
is evident as the number of requested CRISPR plasmids
outpaces other engineering technologies.

6. CRISPR Technologies at Addgene
Visualization of the major CRISPR technologies by function in Addgene’s
collection ordered from left to right by frequency of requests.
The CRISPR collection contains >550 plasmids contributed by 68 depositing labs.

7. Synthetic Biology Technologies at Addgene
Visualization of SynBio tools in Addgene’s collection grouped from left to right
by frequency of requests.
The entire Addgene SynBio collection includes > 1,250 plasmids contributed by
46 depositing labs.

8. Syn Bio Depositing Laboratories
Hal Alper
Christopher Anderson
Adam Arkin
Gabor Balazsi
Matthew Bennett
Carolyn Bertozzi
Nicolas Buchler
Cynthia Collins
James Collins
VÃctor de Lorenzo
Colin Dolphin
Tom Ellis
Drew Endy
Susan Golden
John Gray
Je Hasty
Joshua Heazlewood
Gabor Igloi
Farren Isaacs
Dieter Jahn
Alfonso Jaramillo
Roman Jerala
Jay Keasling
Brian Kuhlman
Taek Soon Lee
Joshua Leonard
Han Lim
Wendell Lim
Michela Lizier
Michel Maharbiz
Thorsten Mascher
Ichiro Matsumura
Anastasios Melis
Bradley Moore
Aindrila
Mukhopadhyay
Richard Murray
Erin OShea
Antonio Richart
Herbert Sauro
Claudia Schmidt-
Dannert
Julian Schroeder
Pamela Silver
Jerey Tabor
Christopher Voigt
Cliord Wang
Philip Weyman

9. CRISPR Depositing Laboratories
Mario de Bono
Mike Boxem
Simon Bullock
John Calarco
Wenbiao Chen
Qi-Jun Chen
Yonglong Chen
Lionel Christiaen
George Church
Jennifer Doudna
Peter Duchek
Benjamin Ebert
Andrew Fire
Klaus Foerstemann
Hodaka Fujii
Wataru Fujii
Caixia Gao
Charles Gersbach
Bob Goldstein
Manuel Goncalves
Izuho Hatada
Akitsu Hotta
Shenglin Huang
Xingxu Huang
Danwei Huangfu
Masahito Ikawa
Tyler Jacks
Rudolf Jaenisch
Renjie Jiao
Keith Joung
Sophien Kamoun
Iskra Katic
Hyongbum Kim
Jin-Soo Kim
Masato Kinoshita
David Liu
Ji-Long Liu
Sebastian Lourido
Timothy Lu
Luciano Marrani
Craig Mello
Kiran Musunuru
Kate O'Connor-
Giles
Wayne Parrott
Stanley Qi
David Root
David Sabatini
Alex Schier
Jen Sheen
David Sibley
William Simonds
Paul Sternberg
Robert Stupar
James Thomson
Ron Vale
Wensheng Wei
Jonathan
Weissman
Susan Wente
Jill Wildonger
Scot Wolfe
Takashi Yamamoto
Yinong Yang
Kosuke Yusa
Donald Zack
Mikel Zaratiegui
Feng Zhang
Huimin Zhao
Yunde Zhao

10. Addgene Statistics
>35,000 plasmids stored
>470,000 plasmids shipped to >5,000 institutions in 78 countries
From >2,100 contributing labs, >500 institutions worldwide
>2,000 plasmids shipped each week
51% shipped to scientists outside the United States
“I do science differently because
I can use the Addgene library to
find reagents”

11. Benefits of a Central Plasmid Repository
Save time on request mailing
Access all plasmids in one request
No loss due to turnover of lab members
New labs hear about your work, more citations
Archive historical clones and standards, rigorous QC
Get a list of who has your plasmids any time (for grants or
Tech Transfer offices)
Collaborate! Participate in our Community!

12. Learn More About Addgene
Addgene accelerates research and discovery by
helping researchers easily share information,
access useful research materials, and increase
collaborations. Contributing plasmids to
Addgene’s collection is free and easy.
To learn more about the benefits of depositing to
and requesting from Addgene’s collection, visit
www.addgene.org