A substance that initiates or modifies the rate of a chemical reaction in a living body. A substance that initiates or modifies the rate of a chemical reaction in a living body. Co Enzyme: Organic molecules that transport chemical group. Co Factor: metal ion that certain enzymes need in order to catalyze a reaction FACTORS EFFECTING ENZYME ACTIVITY Temperature Substrate Concentration Enzyme Concentration Co Factor Inhibitors

1. ENZYMES
ADNAN MURAD BHAYO
U N I V E R S I T Y O F K A R A C H I , P A K I S T A N

3. HISTORY

4. WILHELM KUHNE

5. LOUIS PASTEUR

6. 1 1946
James B.
Sumner
John H.
Northrop
Wendell M. Stanley

7. STRUCTUREOFENZYME
Made up from 62 to 2500 Amino
Acids
A complex 3-D structure
They have a globular shape

8. ACTIVE SITE
The active site is a small port in an enzyme
where substrate molecules bind and undergo a
chemical reaction.
Active Site is made from 3 to 5 Amino Acids

9. ENZYMES PARTNERS
Co Enzyme:
Organic molecules that transport chemical
group.
Co Factor:
metal ion that certain enzymes need in
order to catalyze a reaction
A holoenzyme is an enzyme with any metal ions or
coenzymes attached to it that is now ready to catalyze a
reaction.

10. LOCK AND KEY MODEL
Active Site and the substrate are
Exactly Complementry

11. INDUCED FIT MODEL
Active Site is Flexible and is not Exactly
Complementry

12. ACTIVATION ENERGY

13. CLASSIFICATION OF ENZYMES

15. OXIDO REDUCTASE
:

16. TRANSFERASE:

17. HYDROLASES

18. LYASES

19. LIGASE

20. ISOMERASE

21. FACTORS EFFECTING ENZYME
ACTIVITY
1. Temperature
2. Substrate Concentration
3. Enzyme Concentration
4. Co Factor
5. Inhibitors

22. BIOCATALYST
VS
CHEMICAL CATALYST