Sophomore Proposal Presentation 03172011

Team KERMIT Research Proposal Amy Chen Christina Gonzalez David Hu Richa Kalsi Tanya Kapoor HaeMin Park Sam Park Alex Proctor Alec Ridgway Andrew Taverner Nick Vujcic Mentor: Steve Turley Librarian: Jim Miller

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[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Intersex tissue: oocytes mixed with seminiferous tubules.

It has been shown that at 200ng/L, feminization effects are visible. ,[object Object],CONCENTRATION of Estradiol LOCATION 5-25 ng/L Pond that received runoff (general) 19 to 75 ng/L MD Eastern Shore streams/ rivers receiving agricultural runoff 20-2330 ng/L Runoff near litter pastures 38.7-196 ng/L Runoff from freshly manured field 245 ng /L Pond following a rain event after poultry litter application 730 ng/L Eluant from pile of poultry litter

What has not done yet been done… ,[object Object],[object Object],[object Object]

Will exposure to environmentally relevant concentrations of PLAH in poultry litter negatively affect the sexual development of Xenopus laevis ?

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],X. Laevis tadpole X. Laevis adults; female on left and male on right

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Days 1-30 Days 30-120 Send 2 homogenized, aqueous subsamples to setup initial concentrations Send 2 subsamples for analysis every week Send 2 subsamples for analysis biweekly

[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],0 ng/L estradiol 15 ng/L 60 ng/L 300 ng/L 25 tadpoles 25 tadpoles 25 tadpoles 25 tadpoles X 5 replicates

5 DPF: expose to poultry litter 17 DPF: sex differentiation sensitive stage 55-75 DPF: Metamorphosis occurs. 120 DPF: Sacrifice juvenile frogs with tricaine methanesulfonite (MS-222) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Sex Differentiation Determination ,[object Object],[object Object],[object Object],[object Object]

Statistical Analysis Shapiro Wilk’s Test for Normality Bartlett’s Test for Normality Steel’s Many-One Rank Test (non parametric test) Dunnett’s Test (Parametric test) Normal Non-normal Homogenous variance Heterogenous variance

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Fall 2011 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Fall 2012 Spring 2012 ,[object Object],[object Object],[object Object],[object Object],Spring 2013 * ,[object Object],[object Object],*Provided world does not end in 2012

Item Quantity Unit Cost Overall Cost 5-Gallon Aquaria 20 Donated $0.00 Histology Materials n/a n/a $500.00 X. Laevis Tadpole and Adult Powdered Food n/a n/a $40.00 Tank Maintenance Items n/a n/a $210.00 Poultry Litter I pile n/a <$50.00 X. Laevis Specimens 400 Donated $0.00 pH/ DO2 1 $500 $500 Lighting 20 $10 $200 Lab Space 1 n/a $0.00 Poultry Litter Analyses 25 300 $7500.00 TOTALS ~$9000.00

References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

Sophomore Proposal Presentation 03172011

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