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Meat Science 60 (2002) 379–387
                                                                                                                www.elsevier.com/locate/meatsci




                    Eating quality of lamb meat: effects of breed, sex,
                     degree of maturity and nutritional management
                           G. Arsenos*, G. Banos, P. Fortomaris, N. Katsaounis,
                                 C. Stamataris, L. Tsaras, D. Zygoyiannis
         Department of Animal Husbandry, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece

                            Received 28 January 2001; received in revised form 16 June 2001; accepted 16 June 2001




Abstract
   The eating quality of lamb meat of three indigenous Greek dairy breeds of sheep, reared under different nutritional management
conditions, was assessed by Taste Panel Tests (TPT). Lean samples from the leg joints of 69 lamb carcasses of the Boutsko (B),
Serres (S) and Karagouniko (K) breed were used. For the first TPT, 24 lambs (four males and four females of each breed) were
reared in individual pens on a concentrate ration fed ad libitum. For the second TPT, 27 individual penned male lambs were fed on
Lucerne hay ad libitum and on three different levels of concentrate: High (H), Medium, (M) and Low (L). For the third TPT, 18
male lambs were used; initially the groups were fed indoors for 63 days on three different levels of concentrate (H, M and L)
together with ad libitum Lucerne hay, and subsequently finished on irrigated, sown pasture. For TPT 1, leg joints were obtained
from lambs that had been slaughtered at 30, 45, 60 or 90% of mature weight (PMW) for each breed. For TPT 2, lambs were
slaughtered at 23, 28 or 33 kg target slaughter live weights (TSLW), common for all breeds. For TPT 3, lambs were slaughtered at
48 or 55% of PMW for each breed. Panellists assessed warm, roasted lean samples of leg joints and rated Flavour, Juiciness, Ten-
derness and Overall Acceptability. In TPT 1, degree of maturity significantly affected Flavour, Tenderness and Overall Accept-
ability whereas sex affected only Flavour. In TPT 2, breed and concentrate level significantly affected the quality characteristics, but
concentrate level had no significant effect in TPT 3. Effects associated with slaughter weight were significant for most characteristics
in all TPT, with decreasing acceptability for older/heavier lambs. Significant interactions were found between breed and the other
factors in TPT 2 and TPT 3 for most quality characteristics. Results from these studies suggest changing traditional production
systems may enhance the eating quality of lamb meat. # 2002 Elsevier Science Ltd. All rights reserved.
Keywords: Lamb; Meat; Quality; Taste panel



1. Introduction                                                             at low weight has led to considerable research effort
                                                                            over the past few years (Arsenos, 1997; Arsenos,
  Quality of lamb meat is of great interest to producers,                   Zygoyjannis, Kufidis, Katsaounis, & Stamataris, 2000;
consumers and scientists. There were a large amount of                      Boutonnet, 1999; Dransfield et al., 2000; Rubino, Mor-
studies carried out in the UK, Australia and New Zeal-                      and-Fehr, Renieri, Peraza, & Sarti, 1999; Sanudo,
and several years ago (e.g. Cramer, Barton, Shorland, &                     Alfonso, Sanchez, Delfa, & Texeira, 2000; Sanudo,
Czochanska, 1967; Kemp, Johnson, Stewart, Ely, & Fox,                       Sanchez, & Alfonso, 1998b; Wood, Enser, Fisher, Nute,
1976; Park, Corbett, & Furnival, 1972; Purchas et al.,                      Richardson, & Sheard, 1999; Zygoyiannis, Katsaounis,
1986; Rhodes, 1971). The fact that the carcass and meat                     Stamataris, Arsenos, Tsaras, & Doney, 1999;
qualities of lamb types produced across Member States                       Zygoyiannis, Kyriazakis, Stamataris, Friggens, & Kat-
of the EU are diverse, and the dearth of information                        saounis, 1997). The theme is important on several
particularly from the Mediterranean regions where                           counts. First, it is important for the future of sheep
consumers prefer meat from milk fed lambs slaughtered                       production. Sheep producers operate in a competitive
                                                                            market and their survival is threatened because sheep
  * Corresponding author. Tel.: +30-31-999988; fax: +30-31-                 meat is continually facing challenges to maintain/
999892.                                                                     increase its market share (Boutonet, 1999; Morand-Fehr
    E-mail address: arsenosg@vet.auth.gr (G. Arsenos).                      & Boyazoglu, 1999). Second, it relates to the emergence
0309-1740/02/$ - see front matter # 2002 Elsevier Science Ltd. All rights reserved.
PII: S0309-1740(01)00147-4
380                                    G. Arsenos et al. / Meat Science 60 (2002) 379–387

of specific market needs and trends, implying a product             2.2. Joints and sample preparation
made in a specific area with specific characteristics. If
sheep production is to remain competitive lamb should                 The experimental procedures regarding preparation
meet the demand of individual markets (Rubino et al.,              and presentation were identical for all samples used for
1999; Sanudo, Santolaria, Maria, Osorio, & Sierra,                 TPT. All lambs were weighed at the start of each
1996; Sanudo et al., 1998a,b). Further, markets require            experiment and subsequently at 7-day intervals (every
a constant volume of quality foods and, at the same                Monday). Those found to be approaching pre-defined
time there is a great variation in the perception and              live weights were then weighed daily until the nomi-
definition of sheep meat quality amongst consumers (see             nated live weight was reached. Lambs were then
reviews by Dransfield et al., 1999; Sanudo et al., 1998b;           slaughtered on the same day. After slaughter, hot car-
Wood et al., 1999).                                                cass weight was determined and the carcasses were then
  The prevailing view is that quality is one of the major          left at ambient temperature (around 12  C) for 6 h to
factors that determine the consumer’s acceptability and            avoid cold-shortening. Subsequently, all carcasses were
choice amongst meat from different animal species                   placed in a refrigerator at +1  C and were kept at this
(Homer, Cuthbertson, Homer,  McMenamin, 1997;                     temperature overnight. These carcasses were then sawn
Lucke, 1998; Wood et al., 1999). It has been further               down at the middle line through the centre of the ver-
suggested that detailed information could be helpful in            tebral column. The left hand side (LHS) of each carcass
designing feeding systems and adopt strategies that will           was jointed into leg, chump, loin, best end, breast,
guarantee good quality and hence assure the market-                shoulder, middle neck and, neck joints, following a
ability of lamb meat. Examples include manipulating                standard procedure (MLC, 1989). The leg joints were
post-weaning nutritional management and live weight at             weighed (mean weights were 0.75–1.90 kg), sealed into
slaughter to avoid unwanted traits in lamb meat quality            polyethylene bags under vacuum conditions and stored
in traditional production systems (Arsenos, 1997;                  in a freezer (À25  C) until taste panel evaluation.
Arsenos et al., 2000; Rubino et al., 1999; Sanudo et al.,             Before taste panel evaluation, all frozen joints were
1998b; Wood, Enser, Fischer, Nute, Richardson,                    allowed to thaw for 72 h at 3  C. After thawing, the
Sheard, 1998; Wood et al., 1999; Zygoyiannis et al.,               joints were weighed and sealed into cooking bags. All
1999) and by addressing ecological concerns (i.e.                  joints were cooked at a maximum of 200  C in a fan-
organic farming systems, Enser, Kurt, Chiles, Nute,               assisted electric oven, fitted with external temperature
Wood, 1996; Gibon et al., 1999; Lucke, 1998; Matthes,              controllers, until an internal temperature of 75  C was
Hillmann, Demise,  Mohring, 1998).                                reached. Temperature was monitored by a probe, inser-
  This study was part of a project designed to assess the          ted periodically into the centre of each joint. Cooking
quality and marketability of sheep meat produced in the            time of each sample was estimated to be 50 min per kg
less favoured areas of the European Community                      plus 25 min. Following cooking, the cooking bag was
(Arsenos et al., 2000; Zygoyiannis et al., 1997, 1999).            removed and each joint was re-weighed, wrapped in
The objective of the study was to assess, in a series of           aluminium foil and placed in a warming cabinet (55  C).
Taste Panel Tests (TPT), the eating quality of lamb                Thereafter, joints were removed from the warming
meat of three indigenous Greek dairy breeds of sheep               cabinet and treated similarly to chops in strict rotation
reared under different nutritional management and                   to reflect the order that they were placed in the cabinet.
slaughtered at different live weights using standard                Lean cubes, 2Â2Â2 cm in size, subject to panel taste
commercial procedures.                                             evaluation were cut from M. adductor (n=9), M.
                                                                   femoris (n=5) and M. semitendinosus (n=6). These
                                                                   muscles were trimmed of exterior fat and cut into cubes
2. Materials and methods                                           that were subsequently foil wrapped and kept warm
                                                                   prior to serving for taste panel evaluation.
2.1. Source of carcasses
                                                                   2.3. TPT 1
  The study was carried out using lean samples from the
leg joints of 69 lamb carcasses. They belonged to three              The first Taste Panel Test (TPT 1) was performed
indigenous dairy breeds of sheep, which are the most               using lean samples from the leg joints of 24 lambs, 12
representative breeds in Greece: the Boutsko breed                 male and 12 female, of each of the B, S and K breeds.
(B), the Serres breed (S), and the Karagouniko breed               They were reared on ad libitum fresh concentrate feed
(K). For details on the growth and meat production                 (pellets) together with 100 g/day of unchopped Lucerne
potential of these breeds see Zygogiannis et al. (1997).           hay (Zygoyiannis et al., 1997). Lambs were slaughtered
All lambs were weaned at approximately 42 days of                  at four different live weights, corresponding to four
age and were then reared under different nutritional                proportions of the estimated mature weight (PMW) of
management.                                                        each breed, specifically at 30, 45, 60 and 90% of mature
G. Arsenos et al. / Meat Science 60 (2002) 379–387                                  381

weight (Zygoyiannis et al., 1997). Eating quality char-              2.6. Assessment of eating quality
acteristics of meat from these lambs was assessed in a total
of 480 lean samples from roasted leg joints representing                Eating quality characteristics were assessed in a series
all possible combinations of sex, PMW and breed.                     of TPT. The panellists involved in those tests were
                                                                     typical consumers (30 to 55 years old) of lamb meat and
2.4. TPT 2                                                           were selected on a voluntary basis. Ten panellists were
                                                                     involved in TPT 1 and 20 in TPT 2 and TPT 3, respec-
   The second Taste Panel Test (TPT 2) was performed                 tively. A common scoring system was used in all assess-
using lean samples from the leg joints of totally 27 male            ments. The eating quality was evaluated with an eight-
lambs of the B, S and K breed. They were reared con-                 point descriptive scale (1–8) with larger scores indicating
currently, under the same conditions, with the lambs in              a more favourable rating. Four attributes were rated:
the experiment reported by Zygoyiannis et al. (1999).                Flavour, Juiciness, Tenderness, and Overall Accept-
The lambs were randomly allocated to three target                    ability, which summarised the previous three attributes.
slaughter live weights (TSLW), specifically at 23, 28 and             For example, Flavour was described as: 1=extremely
33 kg, common to all breeds. The TSLWs were selected                 undesirable, 2=very undesirable, 3=moderately unde-
to correspond with the carcass weights at which the B, S             sirable, 4=slightly undesirable, 5=slightly desirable,
and K lambs, respectively, were found to produce the                 6=moderately desirable, 7=very desirable, 8=extre-
most marketable carcasses (Zygogiannis et al., 1997).                mely desirable. Similarly, Juiciness was assessed as
All lambs were offered ad libitum Lucerne hay and were                1=extremely dry to 8=extremely juicy, whereas Ten-
further allocated to three feeding treatments (FT; one               derness was assessed from 1=extremely tough to
lamb per treatment). Concentrate allowances dis-                     8=extremely Tender and Overall Acceptability was
tinguished between these FT and were set at high (H),                personally rated from 1=extremely undesirable to
medium (M) and low (L) levels (Zygoyiannis et al.,                   8=extremely desirable. Panellists were given instruc-
1999). The lambs were slaughtered when they had                      tions for completing the score sheet and were also
reached their respective TSLW. Eating quality char-                  trained in standard attribute assessment. In order to
acteristics of meat from these lambs was assessed in a               ensure that the panellists would not receive the samples
total of 540 lean samples from the leg joint, representing           in the same order we used the statistical designs descri-
all possible combinations of TSLW, FT and breed.                     bed by Nute (1986). Hence, the order of sample pre-
                                                                     sentation in each session was balanced representing all
2.5. TPT 3                                                           factors studied. The samples were offered warm
                                                                     (approximately 30  C) at intervals of 10 min between
   The third Taste Panel Test (TPT 3) was performed                  consecutive sample assessments. Panellists were instruc-
using lean samples from the leg joint of totally 18 male             ted to eat a small piece (3Â3 cm) of unsalted white
lambs of the B, S and K breed. They were reared in two               bread and to rinse their mouth with water between each
phases: an indoor and a grazing phase. During the                    sample assessment. This was considered necessary in
indoor phase the lambs were randomly allocated to                    order to remove any traces of the previous sample from
three FT common for each breed. The design of such                   their mouth. The panelling room was kept free of non-
treatments was similar to that of lambs used for TPT 2               sample odours. Artificial lighting was used and the
with differences only accounting for the levels of con-               temperature was kept constant (about 18  C). Eight
centrate allowances (Zygoyiannis et al., 1999). Within               samples were served per session during TPT 1 and nine
the three feeding treatments, lambs of each breed were               samples per session during TPT 2 and TPT 3. Each ses-
assigned to reach two pre-defined live weights after                  sion was conducted on a daily basis. TPT1 and TPT 2
turnout to the grazing phase, corresponding to 48 and                were each completed in 3 days and TPT 3 in two.
55% of the estimated mature weight of each breed. The
irrigated sown pasture, used in the grazing phase, con-              2.7. Statistical analysis
sisted of tetraploid ryegrass (Lolium perenne) and white
clover (Trifolium repens) in a proportion of 10:1. Pas-                All statistical analyses were performed using GEN-
ture management was based on maintaining a relatively                STAT version 5 (Lawes Agricultural trust, 1993). Data
constant sward surface height (mean Æ S.D.: 6 Æ 2 cm)                were analysed separately for each TPT by using an
throughout the grazing period either by changes in                   analysis of variance (ANOVA) model. The model used
grazing area or by adding non-experimental lambs when                for data analysis of each TPT accounted for possible
required. Lambs were to be slaughtered off pasture                    differences between the panellists (Horgan  Sward,
when they reached their pre-defined live weight. Eating               1995). Random effects were also fitted for panellists
quality of meat from these lambs was assessed in a total             within session. For eating quality characteristics of
of 360 samples representing all possible combinations of             lambs assessed in TPT 1, the model included the effects
FT, PMW and breed.                                                   of breed, sex, PMW and all possible interactions. The
382                                            G. Arsenos et al. / Meat Science 60 (2002) 379–387

model used for the analysis of TPT 2 data included the                     the other hand, there were several statistically significant
effects of breed, FT, TSLW and all possible interac-                        interactions between breed and FT and between breed
tions. Similarly, for TPT 3 data, the model included the                   and slaughter weight (Table 1). Such interactions might
effects of breed, FT, PMW and all possible interactions.                    have considerable practical relevance to lamb meat
                                                                           production systems.
                                                                             As shown in Table 2 the differences in the overall
3. Results                                                                 scores of all four eating quality traits were such as to be
                                                                           detected by consumers eating the lamb in their own
3.1. General                                                               homes.

   A summary of the ANOVA results regarding effects                         3.2. TPT 1
on the four eating quality characteristics (Flavour, Jui-
ciness, Tenderness and Overall Acceptability) of lamb                        Table 3 shows marginal means and standard errors
meat assessed in the three TPT is given in Table 1. R-                     (S.E.) of scores for the four eating quality characteristics
squared values of all analyses ranged from 0.43 to 0.58.                   of lamb meat assessed in TPT 1. Results are shown by
   Average scores of the four eating quality character-                    sex and PMW, collectively for the three breeds.
istics of lamb meat from the B, S and K breed, across                        Slaughter weight, as a proportion of the estimated
the three TPT, are shown in Table 2.                                       mature weight, had significant effects on Flavour, Ten-
   Significant differences (P  0.05–P  0.01) between                       derness and Overall Acceptability, with the largest
breed effects on Flavour, Juiciness, Tenderness and                         PMW (90% of estimated mature weight) receiving con-
Overall Acceptability were detected in TPT 2, indicating                   siderably lower scores. The weight at slaughter corre-
preference for lamb meat from the Karagouniko breed.                       sponded to a commercial range of live weight from
However, such differences were not significant for sam-                      13.3–70 kg (with carcass weight ranging from 6.2 to 31.2
ples assessed in TPT 1 and TPT 3 (Tables 1 and 2). On                      kg). Across sex and breed, Flavour mean scores were

Table 1
Summary of analysis of variance (ANOVA) results regarding effects on four eating quality characteristics (Flavour, Juiciness, Tenderness and
Overall Acceptability) of lamb meat assessed in three Taste Panel Tests (TPT)a

Factors                                            Flavour                Juiciness                 Tenderness         Overall acceptability

TPT 1
Breed                                              NS                     NS                        NS                 NS
Sex                                                *                      NS                        NS                 NS
Slaughter weight (PMW)b                            *                      NS                        **                 *
BreedÂSex                                          NS                     NS                        NS                 NS
BreedÂPMW                                          NS                     NS                        NS                 NS
SexÂPMW                                            *                      NS                        NS                 NS
BreedÂSexÂPMW                                      NS                     NS                        NS                 NS

TPT 2
Breed                                              ***                    ***                       **                 **
Feeding treatment (FT)                             NS                     *                         **                 ***
Target slaughter live weight (TSLW)                NS                     NS                        NS                 ***
BreedÂFT                                           **                     NS                        NS                 NS
BreedÂTSLW                                         NS                     NS                        NS                 NS
FTÂTSLW                                            NS                     *                         *                  ***
BreedÂFTÂTSLW                                      ***                    *                         *                  *

TPT 3
Breed                                              NS                     NS                        NS                 NS
Feeding treatment (FT)                             NS                     NS                        NS                 NS
Slaughter weight (PMW)b                            **                     *                         *                  *
BreedÂFT                                           ***                    NS                        NS                 NS
BreedÂPMW                                          *                      *                         *                  *
FTÂPMW                                             NS                     NS                        NS                 NS
BreedÂFTÂPMW                                       NS                     NS                        NS                 ***
  a
     NS, non significant.
  b
     The slaughter weight was estimated as a proportion of mature weight of each breed.
  *
     P0.001.
  **
      P 0.01.
  ***
       P 0.05.
G. Arsenos et al. / Meat Science 60 (2002) 379–387                                                  383

6.07, 5.93, 5.64 and 4.08, (S.E.D. 0.092, P  0.001) for                           TPT 2. Results are shown by FT and TSLW, collec-
PMW 30, 45, 60 and 90% of mature weight, respec-                                   tively for the three breeds.
tively. Similarly, Tenderness means were 5.23, 5.14, 4.99                             Feeding treatment affected significantly Juiciness,
and 4.80 (S.E.D 0.128, P  0.01), for the four PMW,                                Tenderness and Overall Acceptability. Panellists seemed
respectively. Overall Acceptability means were 6.18,                               to prefer samples from medium and low levels of con-
6.04, 5.92 and 5.71 (S.E.D. 0.106, P  0.01).                                      centrate. Average scores, across breed and TSLW, for
   Sex of the lamb had a significant effect on Flavour. In                           H, M and L feeding treatments, respectively were: 5.04,
general, the panellists seemed to prefer samples from                              5.56, 5.39 (S.E.D. 0.111, P  0.001) for Juiciness; 5.42,
female lambs, especially when heavier carcasses were                               5.77, 5.79 (S.E.D. 0.128; P  0.01) for Tenderness and
being assessed. Across breed and PMW, Flavour scores                               5.61, 5.93, 5.86; S.E.D. 0.130; P  0.05) for Overall
were 5.18 vs. 5.69 (S.E.D. 0.093, P  0.001) for male and                          Acceptability.
female lambs, respectively. Sex differences for the other                              The interaction between FT and TSLW was significant
characteristics were non-significant.                                               for Juiciness (P  0.001), Tenderness (P  0.01) and
   Sex by PMW interaction was also significant for Fla-                             Overall Acceptability (P  0.05). This indicates that con-
vour, mainly because of the large difference between                                centrate levels in the diet of lambs had a significant effect
sexes at the largest PMW (90% of the estimated mature                              on the eating quality characteristics of meat produced at
weight). All other interactions were non-significant.                               different TSLW. Samples from lambs fed medium levels
                                                                                   of concentrate were rated highest except for those from
3.3. TPT 2                                                                         lambs slaughtered at 28 kg, where highest scores were
                                                                                   observed on feeding low levels of concentrate.
  Table 4 shows marginal means and S.E. of scores for                                 Breed had a significant effect on all characteristics in
eating quality characteristics of lamb meat assessed in                            TPT 2 (Tables 1 and 2) with meat samples from

Table 2
Overall means and standard errors of difference (S.E.D.) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Accept-
ability) of lamb meat from the Boutsko (B), Serres (S) and Karagouniko (K) breeds, assessed in three Taste Panel Tests (TPT 1, TPT 2 and TPT 3)a

                        TPT 1                             S.E.D.     TPT 2                               S.E.D.   TPT 3                            S.E.D.

                        B          S          K                      B             S         K                    B         S            K
                        (n=160)    (n=160)    (n=160)                (n=180)       (n=180)   (n=180)              (n=120)   (n=120)      (n=120)

Flavour                 5.44       5.41       5.44        0.11       5.52          5.83      6.09        0.19     5.49      5.41         5.48      0.24
Juiciness               4.49       4.44       4.49        0.11       5.09          5.22      5.69        0.21     5.17      5.21         5.17      0.22
Tenderness              5.11       4.96       5.05        0.11       5.27          5.72      6.00        0.18     5.34      5.35         5.18      0.25
Overall acceptability   6.04       5.91       5.94        0.09       5.48          5.80      6.12        0.19     5.47      5.37         5.32      0.23
  a
      An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating.


Table 3
Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Acceptability)
of lamb meat assessed in Taste Panel Test 1, by sex (M=Males, F=Female) and slaughter weight (PMW—defined as proportion of mature weight)a

                         PMW

                         30%                                45%                                 60%                                90%

                         M                F                 M                  F                M                 F                M               F


Flavour                   6.10             6.03              5.97               5.90             5.57              5.72             3.07b           5.10
                         (0.17)           (0.16)            (0.16)             (0.14)           (0.16)            (0.16)           (0.12)          (0.14)

Juiciness                 4.57             4.58              4.53               4.52             4.47              4.47             4.35            4.33
                         (0.16)           (0.16)            (0.16)             (0.16)           (0.17)            (0.16)           (0.16)          (0.15)

Tenderness                5.23             5.22              5.17               5.12             5.00              4.98             4.77            4.83
                         (0.17)           (0.18)            (0.16)             (0.16)           (0.16)            (0.16)           (0.16)          (0.16)

Overall                   6.18             6.18              6.03               6.05             5.92              5.92             5.67            5.75
Acceptability            (0.16)           (0.16)            (0.17)             (0.16)           (0.16)            (0.15)           (0.14)          (0.14)
  a
      An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating.
  b
      Indicates that means in the same row with different letters differ significantly (P0.05).
384                                                 G. Arsenos et al. / Meat Science 60 (2002) 379–387

Karagouniko lambs scoring the highest. There was also                               Slaughter weight as proportion of the mature weight
a significant three-way interaction between breed,                                 had a significant effect on all characteristics. Average
TSLW and FT on all characteristics.                                               scores, across breed and FT, for lambs slaughtered at 48
                                                                                  and 55% of the mature weight, respectively were: 5.29
3.4. TPT 3                                                                        and 5.63 (S.E.D. 0.140 P 0.05) for Flavour; 4.92 and
                                                                                  5.44 (S.E.D. 0.122 P  0.001) for Juiciness; 4.98 and 5.60
  Table 5 shows marginal means and S.E. of scores for                             (S.E.D. 0.138 P  0.001) for Tenderness; and 5.15 and
eating quality characteristics of lamb meat assessed in                           5.63 (S.E.D. 0.118 P  0.001) for Overall Acceptability.
TPT 3. The results are shown by breed and PMW, col-                               However, there was a significant interaction (P  0.001)
lectively for the three FT.                                                       between breed and PMW for all characteristics. Samples


Table 4
Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Accept-
ability), of lamb meat assessed in Taste Panel Test 2, by feeding treatment (defined by the level of concentrate in the diet; H=High, M=Medium
L=Low) and target slaughter live weight (TSLW)a

Feeding treatment      TSLW

                       23 kg                                         28 kg                                         33 kg

                       H (n=60)        M (n=60)        L (n=60)      H (n=60)       M (n=60)        L (n=60)       H (n=60)       M (n=60)       L (n=60)

Flavour                 5.63 a          5.97 a          5.95 a        5.70 a         5.70 a          5.95 a         5.68 a         5.95 a         5.80 a
                       (0.18)          (0.16)          (0.16)        (0.17)         (0.17)          (0.14)         (0.18)         (0.16)         (0.15)

Juiciness               4.77a           5.80b           5.35ab        5.07 ab        5.20 ab         5.60 b         5.30 ab        5.68 b         5.23 ab
                       (0.18)          (0.17)          (0.21)        (0.17)         (0.17)          (0.18)         (0.20)         (0.16)         (0.17)

Tenderness              5.17a           6.12b           5.85b         5.47 ab        5.37 ab         5.98 b         5.63 ab        5.82 b         5.55 ab
                       (0.20)          (0.18)          (0.19)        (0.17)         (0.18)          (0.19)         (0.21)         (0.20)         (0.18)

Overall                 5.43a          6.13b               5.88 ab    5.63 ab        5.63 ab         6.02 b        5.77 ab         6.02 b         5.68 ab

Acceptability          (0.17)          (0.17)          (0.19)        (0.15)         (0.15)          (0.16)         (0.18)         (0.16)         (0.16)

a, b Indicate that means in the same row, with different letters differ significantly (P 0.05); letters ab is not statistically different to a and b.
   a
     An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating.




Table 5
Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Acceptability)
of lamb meat assessed in Taste Panel Test 3, by breed (B=Boutsko, S=Serres and K=Karagouniko) and slaughter weight (PMW–defined as pro-
portion of mature weight)a

                        Breed (PMW)

                        B                                              S                                              K

                        48% (n=60)               55% (n=60)            48% (n=60)              55% (n=60)             48% (n=60)              55% (n=60)

Flavour                  5.17 a                   5.82 b                5.57 ab                 5.25 ab                5.15 a                  5.82 b
                        (0.20)                   (0.18                 (0.17)                  (0.19)                 (0.20)                  (0.17)

Juiciness                4.55 a                   5.78 b                5.27 b                  5.15 b                 4.93 ab                 5.40 b
                        (0.23)                   (0.18)                (0.19)                  (0.19)                 (0.19)                  (0.17)

Tenderness               4.50 a                   6.18 b                5.45 c                  5.25 c                 4.98 ac                 5.38 c
                        (0.24)                   (0.17                 (0.19)                  (0.21)                 (0.22)                  (0.18)

Overall                  4.83 a                   6.10 b                5.57 b                  5.18 ab                5.05 ab                 5.60 b
Acceptability           (0.23)                   (0.16                 (0.17)                  (0.20)                 (0.20)                  (0.16)

a,b,c Indicate that means in the same row, with different letters differ significantly (P 0.05); letters ab are not statistically different to a and b; letters
ac are not statistically different to a and c.
  a
     An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating.
G. Arsenos et al. / Meat Science 60 (2002) 379–387                                 385

from heavier PMW scored higher for B and K but lower               Nute, Hogg,  Walters, 1990; Ellis et al., 1997; Sanudo
for S compared with samples from lighter carcasses.                et al., 1998b). Further investigation of this effect was
                                                                   not feasible in the current study because the leg joints
                                                                   used for TPT 2 and TPT 3 were obtained from male
4. Discussion                                                      lambs only.

  The main objective of this study was to investigate the          4.2. Effect of nutritional management
effects of post-weaning nutritional management and live
weight at slaughter on the eating quality of lamb meat                The prevailing view in the literature is that informa-
produced by three Greek dairy breeds of sheep. This                tion on how nutritional management affects eating
was achieved by three taste panel tests. It has been sug-          quality characteristics of meat would help to explain the
gested that information on eating quality characteristics          differences observed in such characteristics of lamb
of lamb meat produced by taste panel assessments could             meat produced under different systems and, hence, pro-
provide important insights on how such characteristics             vide insights for the development of new strategies for
are perceived and further evaluated by consumers                   more profitable production of sheep meat (Boutonnet,
(Sanudo et al., 1996, 1998a,b; Wood et al., 1998).                 1999; Sanudo et al., 1998b; Wood et al., 1999). Results
  Samples used for taste panel assessment represented              from TPT 2 and 3 suggested that there is much to
several combinations of breed, sex, nutritional manage-            choose between breeds of lambs investigated here in
ment conditions and live weights at slaughter. These               terms of eating quality of the meat they produce when
factors are discussed next.                                        manipulation of nutritional management is possible. In
                                                                   particular, results from TPT 2, where lambs were fed on
4.1. Effect of breed and sex                                        three different levels of concentrate throughout the
                                                                   course of the experiment, revealed a significant effect of
   Breed has been suggested as a complex source of var-            such FT on Juiciness, Tenderness and Overall accept-
iation of meat quality whose estimated effect may vary              ability of roasted lean samples of their leg joints. In
depending on the comparison criterion (Sanudo et al.,              contrast, when lambs were reared under a similar nutri-
1998b). Results of the present study clearly demonstrate           tional management for a preliminary period indoors
that breed is a factor affecting the eating quality of lamb         and, subsequently, finished on irrigated sown pasture,
meat. Presence of significant interactions between breed            there were no significant effects of the FT during the
and the other factors studied in each TPT support the              indoor period on any of the eating quality parameters
view that the genetic potential of lambs determines, to a          assessed in TPT 3. The latter would imply that the
certain extent, the quality of the meat they produce               effects of nutritional management of lambs during the
(Arsenos et al., 2000; Carson, Moss, Steen,  Kilpa-               finishing period, here grazing, are likely to overshadow
trick, 1999; Ellis, Webster, Merrell,  Brown, 1997;               any effect of previous FT. In this respect, Crouse, Field,
Matthes et al., 1998; Sanudo, Campo, Sierra, Maria,                Chant, Ferrell, Smith, and Harrison (1978) stated that
Olleta,  Santolaria, 1997). Results also confirmed pre-            improvement of the quality characteristics of sheep
vious suggestions that the indigenous Greek breeds of              meat could be mainly achieved through manipulation of
sheep produced carcasses with a relatively high eating             feeding. More recent studies, using taste panel assess-
quality (Arsenos, 1997; Zygoyiannis, Stamataris,                   ments have also shown that there is a considerable effect
Kouimtzis,  Doney, 1990). However, the fact that                  of nutritional management in post-weaning nutrition of
breed as a main effect was significant only for eating               lambs on the quality of meat they produce (Sanudo et
quality characteristics assessed in TPT 2 indicated that           al., 1998b; Vipond, Marie,  Hunter, 1995).
breed is not necessarily a dominant factor with respect
to the eating quality of lamb meat. Alfonso (2000) and             4.3. Effect of live weight at slaughter
Notter et al. (1991) suggested that the breed effect on
meat quality was not very important compared with                    The evidence in the literature regarding effects of live
such factors as feeding treatment.                                 weight at slaughter on the quality of lamb meat is not
   A question, addressed only in TPT 1, was whether the            very conclusive (Hopkins, Beattie,  Pirlot, 1998;
sex of lambs, slaughtered at different live weights repre-          Keane  Allen, 1998; Sanudo et al., 1996, 1997, 1998b;
senting different degrees of maturity, affected the eating           Vergara, Molina,  Gallego, 1999; Zygoyiannis et al.,
quality characteristics of meat. Results of TPT 1 did not          1999). Live weight at slaughter, defined either as target
reveal significant effects of sex on eating quality char-            weight corresponding to marketable carcasses or as
acteristics, except for Flavour, where female lambs gen-           proportion of mature weight, affected significantly most
erally gave more desirable meat than males. This is in             eating quality characteristics assessed by the taste panel
agreement with the view that differences between sexes              tests. For the range of PMW considered in this study,
in meat quality are not very important (Dransfield,                 preferred samples were those of lambs slaughtered at
386                                      G. Arsenos et al. / Meat Science 60 (2002) 379–387

low live weight when the nutritional management was                  tion of lambs and their live weight at slaughter. Given
ad libitum concentrate feed (TPT 1). This is not sur-                the results of the current study the expectation is that
prising given the overwhelming market desire for light               consumers may be able to differentiate meat samples on
carcasses in Greece (Zygoyiannis et al., 1997) and most              the basis of these qualities.
likely across the Mediterranean countries, since it is
believed that meat from animals slaughtered at low
weights is more tender than meat from heavier lambs                  Acknowledgements
(Sanudo et al., 1997, 1998a,b; Vergara et al., 1999).
   Results from TPT 2 suggest that when concentrate                    The work was supported by the European Commu-
allowances in the diet are restricted then it is possible to         nity (DG VI) project No. CAMAR 8001 CT 91-0308) as
produce heavier carcasses that are still highly acceptable           a part of a collaborative program between the UK,
to consumers. However, it should be borne in mind that               Greece and Spain. We are grateful to all those who
lambs used in this study belong to breeds that differ                 participated in the various aspects of this study. Espe-
significantly in their growth potential. For example,                 cially, we would like to thank the farmer Mr. G.
Boutsko lambs were slaughtered after 70–158 days post-               Zygoyiannis and his family for their essential assistance
weaning, depending on the concentrate level of the diet,             on animal and pasture management.
whereas the range was 41–130 days for Serres lambs and
35–97 days for Karagouniko lamb, respectively. There-
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Eating quality of lamp meat

  • 1. Meat Science 60 (2002) 379–387 www.elsevier.com/locate/meatsci Eating quality of lamb meat: effects of breed, sex, degree of maturity and nutritional management G. Arsenos*, G. Banos, P. Fortomaris, N. Katsaounis, C. Stamataris, L. Tsaras, D. Zygoyiannis Department of Animal Husbandry, School of Veterinary Medicine, Aristotle University of Thessaloniki, 54006 Thessaloniki, Greece Received 28 January 2001; received in revised form 16 June 2001; accepted 16 June 2001 Abstract The eating quality of lamb meat of three indigenous Greek dairy breeds of sheep, reared under different nutritional management conditions, was assessed by Taste Panel Tests (TPT). Lean samples from the leg joints of 69 lamb carcasses of the Boutsko (B), Serres (S) and Karagouniko (K) breed were used. For the first TPT, 24 lambs (four males and four females of each breed) were reared in individual pens on a concentrate ration fed ad libitum. For the second TPT, 27 individual penned male lambs were fed on Lucerne hay ad libitum and on three different levels of concentrate: High (H), Medium, (M) and Low (L). For the third TPT, 18 male lambs were used; initially the groups were fed indoors for 63 days on three different levels of concentrate (H, M and L) together with ad libitum Lucerne hay, and subsequently finished on irrigated, sown pasture. For TPT 1, leg joints were obtained from lambs that had been slaughtered at 30, 45, 60 or 90% of mature weight (PMW) for each breed. For TPT 2, lambs were slaughtered at 23, 28 or 33 kg target slaughter live weights (TSLW), common for all breeds. For TPT 3, lambs were slaughtered at 48 or 55% of PMW for each breed. Panellists assessed warm, roasted lean samples of leg joints and rated Flavour, Juiciness, Ten- derness and Overall Acceptability. In TPT 1, degree of maturity significantly affected Flavour, Tenderness and Overall Accept- ability whereas sex affected only Flavour. In TPT 2, breed and concentrate level significantly affected the quality characteristics, but concentrate level had no significant effect in TPT 3. Effects associated with slaughter weight were significant for most characteristics in all TPT, with decreasing acceptability for older/heavier lambs. Significant interactions were found between breed and the other factors in TPT 2 and TPT 3 for most quality characteristics. Results from these studies suggest changing traditional production systems may enhance the eating quality of lamb meat. # 2002 Elsevier Science Ltd. All rights reserved. Keywords: Lamb; Meat; Quality; Taste panel 1. Introduction at low weight has led to considerable research effort over the past few years (Arsenos, 1997; Arsenos, Quality of lamb meat is of great interest to producers, Zygoyjannis, Kufidis, Katsaounis, & Stamataris, 2000; consumers and scientists. There were a large amount of Boutonnet, 1999; Dransfield et al., 2000; Rubino, Mor- studies carried out in the UK, Australia and New Zeal- and-Fehr, Renieri, Peraza, & Sarti, 1999; Sanudo, and several years ago (e.g. Cramer, Barton, Shorland, & Alfonso, Sanchez, Delfa, & Texeira, 2000; Sanudo, Czochanska, 1967; Kemp, Johnson, Stewart, Ely, & Fox, Sanchez, & Alfonso, 1998b; Wood, Enser, Fisher, Nute, 1976; Park, Corbett, & Furnival, 1972; Purchas et al., Richardson, & Sheard, 1999; Zygoyiannis, Katsaounis, 1986; Rhodes, 1971). The fact that the carcass and meat Stamataris, Arsenos, Tsaras, & Doney, 1999; qualities of lamb types produced across Member States Zygoyiannis, Kyriazakis, Stamataris, Friggens, & Kat- of the EU are diverse, and the dearth of information saounis, 1997). The theme is important on several particularly from the Mediterranean regions where counts. First, it is important for the future of sheep consumers prefer meat from milk fed lambs slaughtered production. Sheep producers operate in a competitive market and their survival is threatened because sheep * Corresponding author. Tel.: +30-31-999988; fax: +30-31- meat is continually facing challenges to maintain/ 999892. increase its market share (Boutonet, 1999; Morand-Fehr E-mail address: arsenosg@vet.auth.gr (G. Arsenos). & Boyazoglu, 1999). Second, it relates to the emergence 0309-1740/02/$ - see front matter # 2002 Elsevier Science Ltd. All rights reserved. PII: S0309-1740(01)00147-4
  • 2. 380 G. Arsenos et al. / Meat Science 60 (2002) 379–387 of specific market needs and trends, implying a product 2.2. Joints and sample preparation made in a specific area with specific characteristics. If sheep production is to remain competitive lamb should The experimental procedures regarding preparation meet the demand of individual markets (Rubino et al., and presentation were identical for all samples used for 1999; Sanudo, Santolaria, Maria, Osorio, & Sierra, TPT. All lambs were weighed at the start of each 1996; Sanudo et al., 1998a,b). Further, markets require experiment and subsequently at 7-day intervals (every a constant volume of quality foods and, at the same Monday). Those found to be approaching pre-defined time there is a great variation in the perception and live weights were then weighed daily until the nomi- definition of sheep meat quality amongst consumers (see nated live weight was reached. Lambs were then reviews by Dransfield et al., 1999; Sanudo et al., 1998b; slaughtered on the same day. After slaughter, hot car- Wood et al., 1999). cass weight was determined and the carcasses were then The prevailing view is that quality is one of the major left at ambient temperature (around 12 C) for 6 h to factors that determine the consumer’s acceptability and avoid cold-shortening. Subsequently, all carcasses were choice amongst meat from different animal species placed in a refrigerator at +1 C and were kept at this (Homer, Cuthbertson, Homer, McMenamin, 1997; temperature overnight. These carcasses were then sawn Lucke, 1998; Wood et al., 1999). It has been further down at the middle line through the centre of the ver- suggested that detailed information could be helpful in tebral column. The left hand side (LHS) of each carcass designing feeding systems and adopt strategies that will was jointed into leg, chump, loin, best end, breast, guarantee good quality and hence assure the market- shoulder, middle neck and, neck joints, following a ability of lamb meat. Examples include manipulating standard procedure (MLC, 1989). The leg joints were post-weaning nutritional management and live weight at weighed (mean weights were 0.75–1.90 kg), sealed into slaughter to avoid unwanted traits in lamb meat quality polyethylene bags under vacuum conditions and stored in traditional production systems (Arsenos, 1997; in a freezer (À25 C) until taste panel evaluation. Arsenos et al., 2000; Rubino et al., 1999; Sanudo et al., Before taste panel evaluation, all frozen joints were 1998b; Wood, Enser, Fischer, Nute, Richardson, allowed to thaw for 72 h at 3 C. After thawing, the Sheard, 1998; Wood et al., 1999; Zygoyiannis et al., joints were weighed and sealed into cooking bags. All 1999) and by addressing ecological concerns (i.e. joints were cooked at a maximum of 200 C in a fan- organic farming systems, Enser, Kurt, Chiles, Nute, assisted electric oven, fitted with external temperature Wood, 1996; Gibon et al., 1999; Lucke, 1998; Matthes, controllers, until an internal temperature of 75 C was Hillmann, Demise, Mohring, 1998). reached. Temperature was monitored by a probe, inser- This study was part of a project designed to assess the ted periodically into the centre of each joint. Cooking quality and marketability of sheep meat produced in the time of each sample was estimated to be 50 min per kg less favoured areas of the European Community plus 25 min. Following cooking, the cooking bag was (Arsenos et al., 2000; Zygoyiannis et al., 1997, 1999). removed and each joint was re-weighed, wrapped in The objective of the study was to assess, in a series of aluminium foil and placed in a warming cabinet (55 C). Taste Panel Tests (TPT), the eating quality of lamb Thereafter, joints were removed from the warming meat of three indigenous Greek dairy breeds of sheep cabinet and treated similarly to chops in strict rotation reared under different nutritional management and to reflect the order that they were placed in the cabinet. slaughtered at different live weights using standard Lean cubes, 2Â2Â2 cm in size, subject to panel taste commercial procedures. evaluation were cut from M. adductor (n=9), M. femoris (n=5) and M. semitendinosus (n=6). These muscles were trimmed of exterior fat and cut into cubes 2. Materials and methods that were subsequently foil wrapped and kept warm prior to serving for taste panel evaluation. 2.1. Source of carcasses 2.3. TPT 1 The study was carried out using lean samples from the leg joints of 69 lamb carcasses. They belonged to three The first Taste Panel Test (TPT 1) was performed indigenous dairy breeds of sheep, which are the most using lean samples from the leg joints of 24 lambs, 12 representative breeds in Greece: the Boutsko breed male and 12 female, of each of the B, S and K breeds. (B), the Serres breed (S), and the Karagouniko breed They were reared on ad libitum fresh concentrate feed (K). For details on the growth and meat production (pellets) together with 100 g/day of unchopped Lucerne potential of these breeds see Zygogiannis et al. (1997). hay (Zygoyiannis et al., 1997). Lambs were slaughtered All lambs were weaned at approximately 42 days of at four different live weights, corresponding to four age and were then reared under different nutritional proportions of the estimated mature weight (PMW) of management. each breed, specifically at 30, 45, 60 and 90% of mature
  • 3. G. Arsenos et al. / Meat Science 60 (2002) 379–387 381 weight (Zygoyiannis et al., 1997). Eating quality char- 2.6. Assessment of eating quality acteristics of meat from these lambs was assessed in a total of 480 lean samples from roasted leg joints representing Eating quality characteristics were assessed in a series all possible combinations of sex, PMW and breed. of TPT. The panellists involved in those tests were typical consumers (30 to 55 years old) of lamb meat and 2.4. TPT 2 were selected on a voluntary basis. Ten panellists were involved in TPT 1 and 20 in TPT 2 and TPT 3, respec- The second Taste Panel Test (TPT 2) was performed tively. A common scoring system was used in all assess- using lean samples from the leg joints of totally 27 male ments. The eating quality was evaluated with an eight- lambs of the B, S and K breed. They were reared con- point descriptive scale (1–8) with larger scores indicating currently, under the same conditions, with the lambs in a more favourable rating. Four attributes were rated: the experiment reported by Zygoyiannis et al. (1999). Flavour, Juiciness, Tenderness, and Overall Accept- The lambs were randomly allocated to three target ability, which summarised the previous three attributes. slaughter live weights (TSLW), specifically at 23, 28 and For example, Flavour was described as: 1=extremely 33 kg, common to all breeds. The TSLWs were selected undesirable, 2=very undesirable, 3=moderately unde- to correspond with the carcass weights at which the B, S sirable, 4=slightly undesirable, 5=slightly desirable, and K lambs, respectively, were found to produce the 6=moderately desirable, 7=very desirable, 8=extre- most marketable carcasses (Zygogiannis et al., 1997). mely desirable. Similarly, Juiciness was assessed as All lambs were offered ad libitum Lucerne hay and were 1=extremely dry to 8=extremely juicy, whereas Ten- further allocated to three feeding treatments (FT; one derness was assessed from 1=extremely tough to lamb per treatment). Concentrate allowances dis- 8=extremely Tender and Overall Acceptability was tinguished between these FT and were set at high (H), personally rated from 1=extremely undesirable to medium (M) and low (L) levels (Zygoyiannis et al., 8=extremely desirable. Panellists were given instruc- 1999). The lambs were slaughtered when they had tions for completing the score sheet and were also reached their respective TSLW. Eating quality char- trained in standard attribute assessment. In order to acteristics of meat from these lambs was assessed in a ensure that the panellists would not receive the samples total of 540 lean samples from the leg joint, representing in the same order we used the statistical designs descri- all possible combinations of TSLW, FT and breed. bed by Nute (1986). Hence, the order of sample pre- sentation in each session was balanced representing all 2.5. TPT 3 factors studied. The samples were offered warm (approximately 30 C) at intervals of 10 min between The third Taste Panel Test (TPT 3) was performed consecutive sample assessments. Panellists were instruc- using lean samples from the leg joint of totally 18 male ted to eat a small piece (3Â3 cm) of unsalted white lambs of the B, S and K breed. They were reared in two bread and to rinse their mouth with water between each phases: an indoor and a grazing phase. During the sample assessment. This was considered necessary in indoor phase the lambs were randomly allocated to order to remove any traces of the previous sample from three FT common for each breed. The design of such their mouth. The panelling room was kept free of non- treatments was similar to that of lambs used for TPT 2 sample odours. Artificial lighting was used and the with differences only accounting for the levels of con- temperature was kept constant (about 18 C). Eight centrate allowances (Zygoyiannis et al., 1999). Within samples were served per session during TPT 1 and nine the three feeding treatments, lambs of each breed were samples per session during TPT 2 and TPT 3. Each ses- assigned to reach two pre-defined live weights after sion was conducted on a daily basis. TPT1 and TPT 2 turnout to the grazing phase, corresponding to 48 and were each completed in 3 days and TPT 3 in two. 55% of the estimated mature weight of each breed. The irrigated sown pasture, used in the grazing phase, con- 2.7. Statistical analysis sisted of tetraploid ryegrass (Lolium perenne) and white clover (Trifolium repens) in a proportion of 10:1. Pas- All statistical analyses were performed using GEN- ture management was based on maintaining a relatively STAT version 5 (Lawes Agricultural trust, 1993). Data constant sward surface height (mean Æ S.D.: 6 Æ 2 cm) were analysed separately for each TPT by using an throughout the grazing period either by changes in analysis of variance (ANOVA) model. The model used grazing area or by adding non-experimental lambs when for data analysis of each TPT accounted for possible required. Lambs were to be slaughtered off pasture differences between the panellists (Horgan Sward, when they reached their pre-defined live weight. Eating 1995). Random effects were also fitted for panellists quality of meat from these lambs was assessed in a total within session. For eating quality characteristics of of 360 samples representing all possible combinations of lambs assessed in TPT 1, the model included the effects FT, PMW and breed. of breed, sex, PMW and all possible interactions. The
  • 4. 382 G. Arsenos et al. / Meat Science 60 (2002) 379–387 model used for the analysis of TPT 2 data included the the other hand, there were several statistically significant effects of breed, FT, TSLW and all possible interac- interactions between breed and FT and between breed tions. Similarly, for TPT 3 data, the model included the and slaughter weight (Table 1). Such interactions might effects of breed, FT, PMW and all possible interactions. have considerable practical relevance to lamb meat production systems. As shown in Table 2 the differences in the overall 3. Results scores of all four eating quality traits were such as to be detected by consumers eating the lamb in their own 3.1. General homes. A summary of the ANOVA results regarding effects 3.2. TPT 1 on the four eating quality characteristics (Flavour, Jui- ciness, Tenderness and Overall Acceptability) of lamb Table 3 shows marginal means and standard errors meat assessed in the three TPT is given in Table 1. R- (S.E.) of scores for the four eating quality characteristics squared values of all analyses ranged from 0.43 to 0.58. of lamb meat assessed in TPT 1. Results are shown by Average scores of the four eating quality character- sex and PMW, collectively for the three breeds. istics of lamb meat from the B, S and K breed, across Slaughter weight, as a proportion of the estimated the three TPT, are shown in Table 2. mature weight, had significant effects on Flavour, Ten- Significant differences (P 0.05–P 0.01) between derness and Overall Acceptability, with the largest breed effects on Flavour, Juiciness, Tenderness and PMW (90% of estimated mature weight) receiving con- Overall Acceptability were detected in TPT 2, indicating siderably lower scores. The weight at slaughter corre- preference for lamb meat from the Karagouniko breed. sponded to a commercial range of live weight from However, such differences were not significant for sam- 13.3–70 kg (with carcass weight ranging from 6.2 to 31.2 ples assessed in TPT 1 and TPT 3 (Tables 1 and 2). On kg). Across sex and breed, Flavour mean scores were Table 1 Summary of analysis of variance (ANOVA) results regarding effects on four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Acceptability) of lamb meat assessed in three Taste Panel Tests (TPT)a Factors Flavour Juiciness Tenderness Overall acceptability TPT 1 Breed NS NS NS NS Sex * NS NS NS Slaughter weight (PMW)b * NS ** * BreedÂSex NS NS NS NS BreedÂPMW NS NS NS NS SexÂPMW * NS NS NS BreedÂSexÂPMW NS NS NS NS TPT 2 Breed *** *** ** ** Feeding treatment (FT) NS * ** *** Target slaughter live weight (TSLW) NS NS NS *** BreedÂFT ** NS NS NS BreedÂTSLW NS NS NS NS FTÂTSLW NS * * *** BreedÂFTÂTSLW *** * * * TPT 3 Breed NS NS NS NS Feeding treatment (FT) NS NS NS NS Slaughter weight (PMW)b ** * * * BreedÂFT *** NS NS NS BreedÂPMW * * * * FTÂPMW NS NS NS NS BreedÂFTÂPMW NS NS NS *** a NS, non significant. b The slaughter weight was estimated as a proportion of mature weight of each breed. * P0.001. ** P 0.01. *** P 0.05.
  • 5. G. Arsenos et al. / Meat Science 60 (2002) 379–387 383 6.07, 5.93, 5.64 and 4.08, (S.E.D. 0.092, P 0.001) for TPT 2. Results are shown by FT and TSLW, collec- PMW 30, 45, 60 and 90% of mature weight, respec- tively for the three breeds. tively. Similarly, Tenderness means were 5.23, 5.14, 4.99 Feeding treatment affected significantly Juiciness, and 4.80 (S.E.D 0.128, P 0.01), for the four PMW, Tenderness and Overall Acceptability. Panellists seemed respectively. Overall Acceptability means were 6.18, to prefer samples from medium and low levels of con- 6.04, 5.92 and 5.71 (S.E.D. 0.106, P 0.01). centrate. Average scores, across breed and TSLW, for Sex of the lamb had a significant effect on Flavour. In H, M and L feeding treatments, respectively were: 5.04, general, the panellists seemed to prefer samples from 5.56, 5.39 (S.E.D. 0.111, P 0.001) for Juiciness; 5.42, female lambs, especially when heavier carcasses were 5.77, 5.79 (S.E.D. 0.128; P 0.01) for Tenderness and being assessed. Across breed and PMW, Flavour scores 5.61, 5.93, 5.86; S.E.D. 0.130; P 0.05) for Overall were 5.18 vs. 5.69 (S.E.D. 0.093, P 0.001) for male and Acceptability. female lambs, respectively. Sex differences for the other The interaction between FT and TSLW was significant characteristics were non-significant. for Juiciness (P 0.001), Tenderness (P 0.01) and Sex by PMW interaction was also significant for Fla- Overall Acceptability (P 0.05). This indicates that con- vour, mainly because of the large difference between centrate levels in the diet of lambs had a significant effect sexes at the largest PMW (90% of the estimated mature on the eating quality characteristics of meat produced at weight). All other interactions were non-significant. different TSLW. Samples from lambs fed medium levels of concentrate were rated highest except for those from 3.3. TPT 2 lambs slaughtered at 28 kg, where highest scores were observed on feeding low levels of concentrate. Table 4 shows marginal means and S.E. of scores for Breed had a significant effect on all characteristics in eating quality characteristics of lamb meat assessed in TPT 2 (Tables 1 and 2) with meat samples from Table 2 Overall means and standard errors of difference (S.E.D.) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Accept- ability) of lamb meat from the Boutsko (B), Serres (S) and Karagouniko (K) breeds, assessed in three Taste Panel Tests (TPT 1, TPT 2 and TPT 3)a TPT 1 S.E.D. TPT 2 S.E.D. TPT 3 S.E.D. B S K B S K B S K (n=160) (n=160) (n=160) (n=180) (n=180) (n=180) (n=120) (n=120) (n=120) Flavour 5.44 5.41 5.44 0.11 5.52 5.83 6.09 0.19 5.49 5.41 5.48 0.24 Juiciness 4.49 4.44 4.49 0.11 5.09 5.22 5.69 0.21 5.17 5.21 5.17 0.22 Tenderness 5.11 4.96 5.05 0.11 5.27 5.72 6.00 0.18 5.34 5.35 5.18 0.25 Overall acceptability 6.04 5.91 5.94 0.09 5.48 5.80 6.12 0.19 5.47 5.37 5.32 0.23 a An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating. Table 3 Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Acceptability) of lamb meat assessed in Taste Panel Test 1, by sex (M=Males, F=Female) and slaughter weight (PMW—defined as proportion of mature weight)a PMW 30% 45% 60% 90% M F M F M F M F Flavour 6.10 6.03 5.97 5.90 5.57 5.72 3.07b 5.10 (0.17) (0.16) (0.16) (0.14) (0.16) (0.16) (0.12) (0.14) Juiciness 4.57 4.58 4.53 4.52 4.47 4.47 4.35 4.33 (0.16) (0.16) (0.16) (0.16) (0.17) (0.16) (0.16) (0.15) Tenderness 5.23 5.22 5.17 5.12 5.00 4.98 4.77 4.83 (0.17) (0.18) (0.16) (0.16) (0.16) (0.16) (0.16) (0.16) Overall 6.18 6.18 6.03 6.05 5.92 5.92 5.67 5.75 Acceptability (0.16) (0.16) (0.17) (0.16) (0.16) (0.15) (0.14) (0.14) a An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating. b Indicates that means in the same row with different letters differ significantly (P0.05).
  • 6. 384 G. Arsenos et al. / Meat Science 60 (2002) 379–387 Karagouniko lambs scoring the highest. There was also Slaughter weight as proportion of the mature weight a significant three-way interaction between breed, had a significant effect on all characteristics. Average TSLW and FT on all characteristics. scores, across breed and FT, for lambs slaughtered at 48 and 55% of the mature weight, respectively were: 5.29 3.4. TPT 3 and 5.63 (S.E.D. 0.140 P 0.05) for Flavour; 4.92 and 5.44 (S.E.D. 0.122 P 0.001) for Juiciness; 4.98 and 5.60 Table 5 shows marginal means and S.E. of scores for (S.E.D. 0.138 P 0.001) for Tenderness; and 5.15 and eating quality characteristics of lamb meat assessed in 5.63 (S.E.D. 0.118 P 0.001) for Overall Acceptability. TPT 3. The results are shown by breed and PMW, col- However, there was a significant interaction (P 0.001) lectively for the three FT. between breed and PMW for all characteristics. Samples Table 4 Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Accept- ability), of lamb meat assessed in Taste Panel Test 2, by feeding treatment (defined by the level of concentrate in the diet; H=High, M=Medium L=Low) and target slaughter live weight (TSLW)a Feeding treatment TSLW 23 kg 28 kg 33 kg H (n=60) M (n=60) L (n=60) H (n=60) M (n=60) L (n=60) H (n=60) M (n=60) L (n=60) Flavour 5.63 a 5.97 a 5.95 a 5.70 a 5.70 a 5.95 a 5.68 a 5.95 a 5.80 a (0.18) (0.16) (0.16) (0.17) (0.17) (0.14) (0.18) (0.16) (0.15) Juiciness 4.77a 5.80b 5.35ab 5.07 ab 5.20 ab 5.60 b 5.30 ab 5.68 b 5.23 ab (0.18) (0.17) (0.21) (0.17) (0.17) (0.18) (0.20) (0.16) (0.17) Tenderness 5.17a 6.12b 5.85b 5.47 ab 5.37 ab 5.98 b 5.63 ab 5.82 b 5.55 ab (0.20) (0.18) (0.19) (0.17) (0.18) (0.19) (0.21) (0.20) (0.18) Overall 5.43a 6.13b 5.88 ab 5.63 ab 5.63 ab 6.02 b 5.77 ab 6.02 b 5.68 ab Acceptability (0.17) (0.17) (0.19) (0.15) (0.15) (0.16) (0.18) (0.16) (0.16) a, b Indicate that means in the same row, with different letters differ significantly (P 0.05); letters ab is not statistically different to a and b. a An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating. Table 5 Marginal means and standard errors (in parentheses) of four eating quality characteristics (Flavour, Juiciness, Tenderness and Overall Acceptability) of lamb meat assessed in Taste Panel Test 3, by breed (B=Boutsko, S=Serres and K=Karagouniko) and slaughter weight (PMW–defined as pro- portion of mature weight)a Breed (PMW) B S K 48% (n=60) 55% (n=60) 48% (n=60) 55% (n=60) 48% (n=60) 55% (n=60) Flavour 5.17 a 5.82 b 5.57 ab 5.25 ab 5.15 a 5.82 b (0.20) (0.18 (0.17) (0.19) (0.20) (0.17) Juiciness 4.55 a 5.78 b 5.27 b 5.15 b 4.93 ab 5.40 b (0.23) (0.18) (0.19) (0.19) (0.19) (0.17) Tenderness 4.50 a 6.18 b 5.45 c 5.25 c 4.98 ac 5.38 c (0.24) (0.17 (0.19) (0.21) (0.22) (0.18) Overall 4.83 a 6.10 b 5.57 b 5.18 ab 5.05 ab 5.60 b Acceptability (0.23) (0.16 (0.17) (0.20) (0.20) (0.16) a,b,c Indicate that means in the same row, with different letters differ significantly (P 0.05); letters ab are not statistically different to a and b; letters ac are not statistically different to a and c. a An eight-point rating scale (1–8) was used with higher scores indicating more favourable rating.
  • 7. G. Arsenos et al. / Meat Science 60 (2002) 379–387 385 from heavier PMW scored higher for B and K but lower Nute, Hogg, Walters, 1990; Ellis et al., 1997; Sanudo for S compared with samples from lighter carcasses. et al., 1998b). Further investigation of this effect was not feasible in the current study because the leg joints used for TPT 2 and TPT 3 were obtained from male 4. Discussion lambs only. The main objective of this study was to investigate the 4.2. Effect of nutritional management effects of post-weaning nutritional management and live weight at slaughter on the eating quality of lamb meat The prevailing view in the literature is that informa- produced by three Greek dairy breeds of sheep. This tion on how nutritional management affects eating was achieved by three taste panel tests. It has been sug- quality characteristics of meat would help to explain the gested that information on eating quality characteristics differences observed in such characteristics of lamb of lamb meat produced by taste panel assessments could meat produced under different systems and, hence, pro- provide important insights on how such characteristics vide insights for the development of new strategies for are perceived and further evaluated by consumers more profitable production of sheep meat (Boutonnet, (Sanudo et al., 1996, 1998a,b; Wood et al., 1998). 1999; Sanudo et al., 1998b; Wood et al., 1999). Results Samples used for taste panel assessment represented from TPT 2 and 3 suggested that there is much to several combinations of breed, sex, nutritional manage- choose between breeds of lambs investigated here in ment conditions and live weights at slaughter. These terms of eating quality of the meat they produce when factors are discussed next. manipulation of nutritional management is possible. In particular, results from TPT 2, where lambs were fed on 4.1. Effect of breed and sex three different levels of concentrate throughout the course of the experiment, revealed a significant effect of Breed has been suggested as a complex source of var- such FT on Juiciness, Tenderness and Overall accept- iation of meat quality whose estimated effect may vary ability of roasted lean samples of their leg joints. In depending on the comparison criterion (Sanudo et al., contrast, when lambs were reared under a similar nutri- 1998b). Results of the present study clearly demonstrate tional management for a preliminary period indoors that breed is a factor affecting the eating quality of lamb and, subsequently, finished on irrigated sown pasture, meat. Presence of significant interactions between breed there were no significant effects of the FT during the and the other factors studied in each TPT support the indoor period on any of the eating quality parameters view that the genetic potential of lambs determines, to a assessed in TPT 3. The latter would imply that the certain extent, the quality of the meat they produce effects of nutritional management of lambs during the (Arsenos et al., 2000; Carson, Moss, Steen, Kilpa- finishing period, here grazing, are likely to overshadow trick, 1999; Ellis, Webster, Merrell, Brown, 1997; any effect of previous FT. In this respect, Crouse, Field, Matthes et al., 1998; Sanudo, Campo, Sierra, Maria, Chant, Ferrell, Smith, and Harrison (1978) stated that Olleta, Santolaria, 1997). Results also confirmed pre- improvement of the quality characteristics of sheep vious suggestions that the indigenous Greek breeds of meat could be mainly achieved through manipulation of sheep produced carcasses with a relatively high eating feeding. More recent studies, using taste panel assess- quality (Arsenos, 1997; Zygoyiannis, Stamataris, ments have also shown that there is a considerable effect Kouimtzis, Doney, 1990). However, the fact that of nutritional management in post-weaning nutrition of breed as a main effect was significant only for eating lambs on the quality of meat they produce (Sanudo et quality characteristics assessed in TPT 2 indicated that al., 1998b; Vipond, Marie, Hunter, 1995). breed is not necessarily a dominant factor with respect to the eating quality of lamb meat. Alfonso (2000) and 4.3. Effect of live weight at slaughter Notter et al. (1991) suggested that the breed effect on meat quality was not very important compared with The evidence in the literature regarding effects of live such factors as feeding treatment. weight at slaughter on the quality of lamb meat is not A question, addressed only in TPT 1, was whether the very conclusive (Hopkins, Beattie, Pirlot, 1998; sex of lambs, slaughtered at different live weights repre- Keane Allen, 1998; Sanudo et al., 1996, 1997, 1998b; senting different degrees of maturity, affected the eating Vergara, Molina, Gallego, 1999; Zygoyiannis et al., quality characteristics of meat. Results of TPT 1 did not 1999). Live weight at slaughter, defined either as target reveal significant effects of sex on eating quality char- weight corresponding to marketable carcasses or as acteristics, except for Flavour, where female lambs gen- proportion of mature weight, affected significantly most erally gave more desirable meat than males. This is in eating quality characteristics assessed by the taste panel agreement with the view that differences between sexes tests. For the range of PMW considered in this study, in meat quality are not very important (Dransfield, preferred samples were those of lambs slaughtered at
  • 8. 386 G. Arsenos et al. / Meat Science 60 (2002) 379–387 low live weight when the nutritional management was tion of lambs and their live weight at slaughter. Given ad libitum concentrate feed (TPT 1). This is not sur- the results of the current study the expectation is that prising given the overwhelming market desire for light consumers may be able to differentiate meat samples on carcasses in Greece (Zygoyiannis et al., 1997) and most the basis of these qualities. likely across the Mediterranean countries, since it is believed that meat from animals slaughtered at low weights is more tender than meat from heavier lambs Acknowledgements (Sanudo et al., 1997, 1998a,b; Vergara et al., 1999). Results from TPT 2 suggest that when concentrate The work was supported by the European Commu- allowances in the diet are restricted then it is possible to nity (DG VI) project No. CAMAR 8001 CT 91-0308) as produce heavier carcasses that are still highly acceptable a part of a collaborative program between the UK, to consumers. However, it should be borne in mind that Greece and Spain. We are grateful to all those who lambs used in this study belong to breeds that differ participated in the various aspects of this study. Espe- significantly in their growth potential. For example, cially, we would like to thank the farmer Mr. G. Boutsko lambs were slaughtered after 70–158 days post- Zygoyiannis and his family for their essential assistance weaning, depending on the concentrate level of the diet, on animal and pasture management. whereas the range was 41–130 days for Serres lambs and 35–97 days for Karagouniko lamb, respectively. There- fore, although lambs were slaughtered at the same References TSLW, their carcasses might differ significantly in terms of the degree of maturity (Zygoyiannis et al., 1997), Alfonso, M. G. (2000). Caracterization sensorial y aceptabilitad de la which is closely associated with meat quality (Sanudo et carne de doce tipos ovinos representatives de distintos sistemas de al., 1998b) and consumer acceptability. production europeos. PhD thesis, University of Saragosa, Spain. Arsenos, G. (1997). The influence of genotype and method of production Results from TPT 3 suggest that the major factor on fatty acids composition of carcass fat of Boutsko, Serres and Kar- affecting the eating quality of lambs was their live agouniko lambs. PhD thesis, Aristotle University of Thessaliniki, weight at slaughter, irrespective of the feeding treatment Greece, pp. 125–126. prior to grazing. The interaction between post-weaning Arsenos, G., Zygoyjannis, D., Kufidis, D., Katsaounis, N., Stama- taris, C. (2000). The effect of breed slaughter weight and nutritional nutritional management and live weight at slaughter of management on cholesterol content of lamb carcasses. Small Rumi- lambs seemed to account for most of the variation in nant Research, 36, 275–283. scores of eating quality characteristics assessed in TPT 3. Boutonnet, J.-P. (1999). Perspectives of the sheep meat world market Consumers demand tender and flavoursome meat on future production systems and trends. Small Ruminant Research, (Boutonnet, 1999; Homer et al., 1997). Similarly, other 34, 189–195. published data, mainly from British and Spanish taste Carson, A. F., Moss, B. W., Steen, R. W. J., Kilpatrick, D. J. (1999). Effects of the percentage of Texel or Rouge de l’Ouest genes panel tests (Sanudo et al., 1998a) indicate that lamb in lambs on carcass characteristics and meat quality. Meat Science, carcasses from non dairy sheep, were rated as low 69, 81–92. quality with respect to Tenderness and Juiciness and Cramer, D. A., Barton, R. A., Shorland, F. B., Czochanska, Z. were not red. The relationship between Overall Accept- (1967). A comparison of the effects of white clover (Trifolium repens) ability and the other characteristics assessed in all TPT and of perennial ryegrass (Lolium perenne) on fat composition and flavour of lamb. Journal of Agricultural Science, 69, 367–373. conducted in the current study is consistent with the Crouse, J. D., Field, R. A., Chant, J. L., Ferrell, C. L., Smith, G. M., findings of those latter studies. Harrison, V. L. (1978). Effect of dietary energy intake on carcass In conclusion, this study extends previous findings composition and palatability of different weight carcasses from ewe (Arsenos et al., 2000; Zygoyiannis et al., 1999) regarding and ram lambs. Journal of Animal Science, 47, 1207–1218. Dransfield, E., Martin, J. F., Fisher, A., Nute, G. R., Zygyiannis, D., the effects of nutritional management on the eating Stamataris, C., Thorkelsson, G., Valdimarsdottir, T., Piasentier, E., quality of roasted lamb meat and, in particular those of Mills, C., Sanudo, C., Alfonso, M. (2000). Home placement test- Greek dairy breeds of sheep. It should be noted here ing of lamb conducted in six countries. Journal of Sensory Studies, that there is a relationship between carcass weight and 15, 421–436. tenderness, which is dependent on the cooling regime Dransfield, E., Nute, G. R., Hogg, B. W., Walters, B. R. (1990). and carcass processing (Taylor, Warkup, Nute, 1995, Carcass and eating quality of ram, castrated ram and ewe lambs. Animal Production, 50, 291–299. ´ Van Oeckel, Warnants, Boucque, 1999). Although Ellis, M., Webster, G. M., Merrell, B. G., Brown, I. (1997). 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