1. Andrology (part 5)

3. Q: How to prepare …… for freezing?
 Na Citrate
 80ml 2.9gm Na Citrate/ 100gm D.W.
 20ml Egg yolk
 7ml glycerol
 Lactose
 75ml ----> 11.5mg lactose/ 100ml D.W.
 20ml Egg yolk
 5ml glycerol
 Tris diluent
 80ml tris diluent
 20ml Egg yolk
 7ml glycerol
Q: Function of egg yolk? P102

5. Straws
Inseminating gun
Plastic sheath

6. Straw filler
For filling of Different volumes of
straws straws: 0.25, 0.5, 1ml
‫دايما هتالقي في‬
‫حاجة بتملي منها‬
‫علشان كده هي‬
filler

8. ‫‪Straw filler‬‬
‫هتالقي في حاجة بنمأل‬
‫منها‬

9. ‫يبيطبع علي‬
‫ال‬
Straw
‫3 حاجات‬ Printing machine
A.I. Center
Bull
number
Date of
processing

10. Printing machine

11. Sealing machine
Q: Sealing by:
• Heat sealing
• Polyvinyl powder
• U/S

13. Tray

14. • 5-7cm above liguid nitrogen
• 7-10min
• Temperature: -120ᵒC
Horizontal freezing of semen

15. Straw in goblets

16. Liquid nitrogen tank

17. Liquid nitrogen tank

18. Temperature= 38ᵒC
for 15-30 sec