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BOLD and the data life cycle Julie K. Stahlhut Biodiversity Institute of Ontario With examples from BOLD 3.0 Beta
Data life cycle: A common model Data Documentation Initiative, 2004 (http://www.ddialliance.org/) Study concept Data collection Data processing Data distribution Data discovery Data analysis Data archiving Repurposing
What does this mean? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Basic life cycle of barcode data
Data generation
Validation ✗ ✗ ✗ Lab technician Campaignmanager You
Upload
Analysis Hypothesis  testing Context (Ecology, geography, phylogeny) ?  Identification  and comparison = ?
Publication Project Access: Make this project  publicly visible x
The true life cycle Validate Re-visit Generate Publish Analyze Upload
Re-visited  data Add value Manage projects Plan research
Project  manager Taxonomist Graduate student ? ? ?
BOLD tools Taxonomic expertise Barcode data expertise Future expert
1.  Adding value to data
Good sequences but no IDs. Sample code Country Identification Identifier Voucher Location 2010-785 Australia Formicidae U. of Adelaide A2011-Q Australia Formicidae U. of Adelaide ZYZ-1450 Australia Camponotus sp. N. Novice U. of Adelaide 2002-18xt Australia Formicidae U. of Adelaide XM20-36 Australia Formicidae U. of Adelaide ANT-480 Australia Formicidae U. of Adelaide
Reference quality barcodes! Sample code Country Identification Identifier Notes 2010-785 Australia Iridomyrmex purpureus A. Phaenogaster 2011-782 Australia Myrmecia rugosa A. Phaenogaster ZYZ-1450 Australia Polyrhachis australis A. Phaenogaster Corrected 20018xt2 Australia Linepithema humile A. Phaenogaster Invasive XM20-36 Australia Strumigenys sp. A. Phaenogaster ANT-480 Australia Rhytidoponera mayri A. Phaenogaster
 
2. Research planning
? ?
Molecular Statistical Ecological
 
BARCODING SPECIES DIVERSITY Tropical vs. temperate rainforest Formicidae vs. Apidae Research Proposal Lorem Ipsum, M.S. Collaborators: C.M. Bees, University of Guelph A. Phaenogaster, University of Adelaide Abstract:  Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.  BARCODE  duis aute irure velit esse dolore eu Introduction:   Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat   BOLD .  Excepteur sint occaecat
3. Project management Lab progress Sequence quality Collection coverage Data updates
Hymenoptera Barcode Group Quarterly Report: C.M. Bees, Manager ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Hymenoptera Bees -> 2100 Wasps -> 2800 Ants -> 1950
Research Project management Education BOLD resources Collaboration
[object Object],[object Object],[object Object],[object Object],[object Object],Generate Validate Upload Analyze Publish Re-visit
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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The True Data Life Cycle in BOLD

  • 1. BOLD and the data life cycle Julie K. Stahlhut Biodiversity Institute of Ontario With examples from BOLD 3.0 Beta
  • 2. Data life cycle: A common model Data Documentation Initiative, 2004 (http://www.ddialliance.org/) Study concept Data collection Data processing Data distribution Data discovery Data analysis Data archiving Repurposing
  • 3.
  • 4. Basic life cycle of barcode data
  • 6. Validation ✗ ✗ ✗ Lab technician Campaignmanager You
  • 8. Analysis Hypothesis testing Context (Ecology, geography, phylogeny) ?  Identification and comparison = ?
  • 9. Publication Project Access: Make this project publicly visible x
  • 10. The true life cycle Validate Re-visit Generate Publish Analyze Upload
  • 11. Re-visited data Add value Manage projects Plan research
  • 12. Project manager Taxonomist Graduate student ? ? ?
  • 13. BOLD tools Taxonomic expertise Barcode data expertise Future expert
  • 14. 1. Adding value to data
  • 15. Good sequences but no IDs. Sample code Country Identification Identifier Voucher Location 2010-785 Australia Formicidae U. of Adelaide A2011-Q Australia Formicidae U. of Adelaide ZYZ-1450 Australia Camponotus sp. N. Novice U. of Adelaide 2002-18xt Australia Formicidae U. of Adelaide XM20-36 Australia Formicidae U. of Adelaide ANT-480 Australia Formicidae U. of Adelaide
  • 16. Reference quality barcodes! Sample code Country Identification Identifier Notes 2010-785 Australia Iridomyrmex purpureus A. Phaenogaster 2011-782 Australia Myrmecia rugosa A. Phaenogaster ZYZ-1450 Australia Polyrhachis australis A. Phaenogaster Corrected 20018xt2 Australia Linepithema humile A. Phaenogaster Invasive XM20-36 Australia Strumigenys sp. A. Phaenogaster ANT-480 Australia Rhytidoponera mayri A. Phaenogaster
  • 17.  
  • 19. ? ?
  • 21.  
  • 22. BARCODING SPECIES DIVERSITY Tropical vs. temperate rainforest Formicidae vs. Apidae Research Proposal Lorem Ipsum, M.S. Collaborators: C.M. Bees, University of Guelph A. Phaenogaster, University of Adelaide Abstract: Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. BARCODE duis aute irure velit esse dolore eu Introduction: Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat BOLD . Excepteur sint occaecat
  • 23. 3. Project management Lab progress Sequence quality Collection coverage Data updates
  • 24.
  • 25. Research Project management Education BOLD resources Collaboration
  • 26.
  • 27.