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Museum Harvesting Jeremy deWaard
Outline ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Home institutions:  BIO  and  Royal BC Museum ; pet group:  looper moths
Main theme ,[object Object],Entomological museum colleagues: John Brown, Don Lafontaine, Rob Cannings, J.F. Landry, John LaSalle, Karen Needham
Deciding which collections to use ,[object Object],[object Object],[object Object],ANIC ,  NHM ,  NMNH
Deciding which collections to use ,[object Object],[object Object],[object Object]
Deciding which collections to use ,[object Object],[object Object],[object Object]
Deciding which collections to use ,[object Object],[object Object]
Deciding which collections to use ,[object Object],[object Object],[object Object],[object Object]
Deciding which collections to use ,[object Object],[object Object],[object Object]
Deciding which specimens/samples to use ,[object Object]
Deciding which specimens/samples to use ,[object Object],[object Object],[object Object],[object Object]
Deciding which specimens/samples to use ,[object Object],[object Object],[object Object],[object Object]
Deciding which specimens/samples to use ,[object Object],[object Object],[object Object],[object Object]
Destructive vs. non-destructive handling ,[object Object],[object Object]
Destructive vs. non-destructive handling ,[object Object],e.g.  Knolke, S. et al 2005. Insect Systematics and Evolution 35: 401-409.
Voucher recovery protocols ,[object Object]
Voucher recovery protocols ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Biological material transfer policies ,[object Object],[object Object]
Case Study - ANIC 2010/2011 ,[object Object],[object Object]
Case Study - ANIC 2010/2011 ,[object Object],[object Object],[object Object],[object Object]
Case Study - ANIC 2010/2011 ,[object Object],[object Object]
Case Study - ANIC 2010/2011 ,[object Object],[object Object],[object Object],[object Object],[object Object]
Case Study - ANIC 2010/2011 ,[object Object],[object Object],[object Object]
Extra thoughts and tips ,[object Object],[object Object],[object Object],[object Object],[object Object]
 
Case Study - ANIC 2010/2011 ,[object Object],[object Object],[object Object]
Case Study - ANIC 2010/2011 Trip breakdown:  ANIC-1 (Oct 2010) 12031 specimens 4334 species ANIC-2 (April 2011) 16351 specimens 5253 species ANIC-3 (Oct 2011) 12635 specimens 4721 species
DNA-friendly Sampling:  Tissue Source  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object], Avoid cross-contamination!
DNA-friendly Collecting: Specimen Fixation DNA preservation (or degradation) starts during collection  (killing method, exposure to elements, etc.)  Chris Meyer: “Get rid of water & shut down nucleases” ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object], NB! Ensure timely preservation adequate for material
Making Collections DNA-friendly: Preservation ,[object Object],[object Object],[object Object],NB! Do not change from one fixative to another! ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Making Collections DNA-friendly: Other Factors  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Jeremy deWaard - Museum Harvesting

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  • 27. Case Study - ANIC 2010/2011 Trip breakdown: ANIC-1 (Oct 2010) 12031 specimens 4334 species ANIC-2 (April 2011) 16351 specimens 5253 species ANIC-3 (Oct 2011) 12635 specimens 4721 species
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Notes de l'éditeur

  1. Expertise is in Nearctic looper moths, so most of my examples will be entomological, and biased towards North America and Australia.
  2. appears to be a recurring phenotype
  3. links
  4. want vouchers to exist in perpetuity
  5. Alcohol Tubes of Lepidoptera (ATOLep) collection
  6. You may not have your own imaging equipment, microscope, laptop, printer, etc. Unambiguous collection codes
  7. Paul Hebert discussing sampling the Lepidoptera in the Australian National Insect Collection with the head, John LaSalle. Of course one of the limits you may want to discuss is the access to sub-sample type specimens.
  8. Highly taxon-specific but I assume this will be covered this afternoon in module 4.
  9. if you’re trying to capture the full scope of genetic variation within a species, you may wish to sample the ‘outlier’ specimens
  10. http://www.mendeley.com/research/a-procedure-for-combined-genitalia-dissection-and-dna-extraction-in-lepidoptera/
  11. Microscopic examination of the specimens revealed that all diagnostic characters were intact after extraction including the presence and the position of chaeta, the tegument granulation and structures. inexpensive protocol involving voucher recovery
  12. links provider/recipient provide policies up front, avoid problems later
  13. Australian National Insect Collection 2010/2011 ‘barcode blitz’
  14. young in terms of specimen age You can see the head of ANIC, John LaSalle, laying out the ground rules
  15. Michelle will go through the various steps in the next talk
  16. Average age of specimens Formalin vapour?