SlideShare une entreprise Scribd logo
1  sur  1
Spatial and Temporal Expression of a Citrate Permease and
                                                                           Aluminium-induced Citrate Exudation in Common Bean
                                                                                              Dejene Eticha1, Andrés F. Rangel1,2, Idupulapati M. Rao3 and Walter J. Horst1
                                     1Institute                           of Plant Nutrition, Leibniz University Hannover, Herrenhaeuser Str. 2, D-30419 Hannover, Germany; e-Mail: dejene.eticha@pflern.uni-hannover.de
                                                                                                              2YARA GmbH & Co. KG, Hanninghof 35, D-48249 Dülmen, Germany
                                                                                                        3International Center For Tropical Agriculture (CIAT) AA 6713, Cali, Colombia


Introduction
Aluminium (Al) toxicity is one of the major limiting factors for plant growth in acid soils especially in the tropics where common bean (Phaseolus vulgaris) is
produced. Al resistance in common bean is conferred by citrate exudation which detoxifies Al in the root apoplast. Citrate exudation starts after a considerable
lag period subsequent to Al treatment indicating that the activation of resistance mechanism involves Al-induced gene expression (Rangel et al., 2010). We
identified a putative citrate permease gene PvMATE (Phaseolus vulgaris Multidrug and Toxin Extrusion) which is highly up regulated by Al treatment (Eticha et
al., 2010). Here, the spatial and temporal expression of PvMATE in the root tip in relation to citrate exudation is presented.

Materials and methods
Two contrasting common bean genotypes, Quimbaya (Al-resistant) and VAX 1 (Al-sensitive) were used to study gene expression as well as citrate exudation of
the different apical root zones.

                                                                                                                                      Results
    Root growth of both genotypes is equally inhibited in short-term Al treatment                                                                  PvMATE is strongly expressed at the root tip compared to the elongation zone. This
1                                                                                                                                              2   correlates with the high citrate exudation at the root apex. Citrate exudation in the
    but genotypic difference is observed in medium- and long-term treatment.
                                                                                                                                                   elongation zone is still substantially high indicating that in common bean, not only
                                                                                                                                                   the transition but also the elongation zone must be protected.




                                                                                                                                                                                                                  Relative expression level
                                                                                                                                                                                                                                              4000
    Relative root elongation (%)




                                   100                                     Quimbaya (R)                                                                                                                                                                                    Control
                                                                           Vax-1 (S)                                                                                                                                                                                       Al [20 µM]
                                                                                                                                                                                                                                              3000

                                   80                                                                                                                                                                                                                                            PvMATE expression
                                                                                                                                                                                                                                              2000

                                   60                                                                                                                                                                                                         1000

                                                                                                                                                                                                                                                0
                                   40
                                                                                                                                                                                                                                               100
                                                                                                                                                                                                                     [pmol (mm root)-1h-1]
                                                                                                                                                                                         Citrate exudation rate



                                   20                                                                                                                                                                                                           80

                                                                                                                                                                                                                                                60
                                                                                                                                                                                                                                                                                 Citrate exudation
                                    0                                                                                                                                                                                                           40
                                              1 2 3 4 5 6 7 8 9 10 12                                   16         20    24                                                                                                                     20
                                                                                     Al treatment time (h)                                                                                                                                       0
                                                                                                                                                                                                                                                          0-3       3-6      6-10
                                                                                                                                                                                                                                                     Distance from the root apex (mm)


        Fig. 1. Root growth of two contrasting common bean genotypes                                                                           Fig. 2. Expression of citrate permease gene (PvMATE) and citrate exudation
                treated with Al for up to 24h.                                                                                                         at different root zones of Al-resistant bean genotype Qimbaya.

    Al-induced expression of STOP1 transcription factor precedes the enhanced                                                                      Citrate exudation began after a delay of about 4 h of Al treatment. It is preceded by
3   expression of PvMATE indicating that STOP1 regulates the PvMATE gene.                                                                      4   enhanced PvMATE expression. Citrate exudation continued to increase in the
                                                                                                                                                   resistant genotype but dropped shortly afterwards in the sensitive genotype.
                                                                         12
                                             Relative expression level




                                                                                     Quimbaya (R)
                                                                         10          Vax-1 (S)                                                                                                5
                                                                                                                                                                                                                                                        Quimbaya (R)
                                                                                                                                                         [nmol (10-mm root tip)-1 h-1]




                                                                          8
                                                                               STOP1                                                                                                                                                                    VAX-1 (S)
                                                                                                                                                            Citrate exudation rate




                                                                          6                                                                                                                   4
                                                                          4
                                                                          2                                                                                                                   3
                                                                          0
                                                                      1000
                                          Relative expression level




                                                                                                                                                                                              2
                                                                         800
                                                                         600
                                                                                                                                                                                              1
                                                                         150   PvMATE
                                                                         100
                                                                                                                                                                                              0
                                                                         50
                                                                          0
                                                                                                                                                                                       control 0-2 2-4 4-6 6-8 8-10
                                                                                 0       1      2       3      4                                                                   Al treatment time & citrate collection period (h)
                                                                                       Al treatment time (h)

        Fig. 3. Gene expression of STOP1 and PvMATE at different                                                                               Fig. 4.   Citrate exudation of contrasting common bean genotypes at different
                durations of Al treatment.                                                                                                               durations of Al treatment.


                                                                         Conclusion
                                                                         In common bean, citrate exudation involves a cascade of events which include the reception of an Al
                                                                         signal, activation of the STOP1 transcription factor, enhancement of citrate permease (pvMATE) gene
                                                                         expression and subsequent citrate exudation resulting in the maintenance of root growth under Al stress.

                                                                               Reference: Eticha et al. (2010) Annals of Botany, 105: 1119-1128.   Rangel et al. (2010) Physiologia Plantarum, 38: 176-190.
                                                                                             The financial support of the German Research Foundation (DFG) is highly acknowledged.

Contenu connexe

Tendances

Education and Work Experience
Education and Work ExperienceEducation and Work Experience
Education and Work Experienceksmujumdar
 
ASMS 2010 poster
ASMS 2010 posterASMS 2010 poster
ASMS 2010 postermadinger
 
The Power of Cost Curves in Business Communication
The Power of Cost Curves in Business CommunicationThe Power of Cost Curves in Business Communication
The Power of Cost Curves in Business CommunicationCerebel Consulting
 
Ge Tetra Led Reference Chart
Ge Tetra Led Reference ChartGe Tetra Led Reference Chart
Ge Tetra Led Reference Chartledlight1
 

Tendances (6)

Sc610 cs1e
Sc610 cs1eSc610 cs1e
Sc610 cs1e
 
Education and Work Experience
Education and Work ExperienceEducation and Work Experience
Education and Work Experience
 
NRTEE: Kirsten Vice
NRTEE: Kirsten ViceNRTEE: Kirsten Vice
NRTEE: Kirsten Vice
 
ASMS 2010 poster
ASMS 2010 posterASMS 2010 poster
ASMS 2010 poster
 
The Power of Cost Curves in Business Communication
The Power of Cost Curves in Business CommunicationThe Power of Cost Curves in Business Communication
The Power of Cost Curves in Business Communication
 
Ge Tetra Led Reference Chart
Ge Tetra Led Reference ChartGe Tetra Led Reference Chart
Ge Tetra Led Reference Chart
 

Plus de CIAT

Agricultura Sostenible y Cambio Climático
Agricultura Sostenible y Cambio ClimáticoAgricultura Sostenible y Cambio Climático
Agricultura Sostenible y Cambio ClimáticoCIAT
 
Resumen mesas trabajo
Resumen mesas trabajoResumen mesas trabajo
Resumen mesas trabajoCIAT
 
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...Impacto de las intervenciones agricolas y de salud para reducir la deficienci...
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...CIAT
 
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...CIAT
 
El rol de los padres en la nutrición del hogar
El rol de los padres en la nutrición del hogarEl rol de los padres en la nutrición del hogar
El rol de los padres en la nutrición del hogarCIAT
 
Scaling up soil carbon enhancement contributing to mitigate climate change
Scaling up soil carbon enhancement contributing to mitigate climate changeScaling up soil carbon enhancement contributing to mitigate climate change
Scaling up soil carbon enhancement contributing to mitigate climate changeCIAT
 
Impacto del Cambio Climático en la Agricultura de República Dominicana
Impacto del Cambio Climático en la Agricultura de República DominicanaImpacto del Cambio Climático en la Agricultura de República Dominicana
Impacto del Cambio Climático en la Agricultura de República DominicanaCIAT
 
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...CIAT
 
Investigaciones sobre Cadmio en el Cacao Colombiano
Investigaciones sobre Cadmio en el Cacao ColombianoInvestigaciones sobre Cadmio en el Cacao Colombiano
Investigaciones sobre Cadmio en el Cacao ColombianoCIAT
 
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue in Colo...
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue    in Colo...Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue    in Colo...
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue in Colo...CIAT
 
Tackling cadmium in cacao and derived products – from farm to fork
Tackling cadmium in cacao and derived products – from farm to forkTackling cadmium in cacao and derived products – from farm to fork
Tackling cadmium in cacao and derived products – from farm to forkCIAT
 
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...CIAT
 
Geographical Information System Mapping for Optimized Cacao Production in Col...
Geographical Information System Mapping for Optimized Cacao Production in Col...Geographical Information System Mapping for Optimized Cacao Production in Col...
Geographical Information System Mapping for Optimized Cacao Production in Col...CIAT
 
Contenido de cadmio en granos de cacao
Contenido de cadmio en granos de cacaoContenido de cadmio en granos de cacao
Contenido de cadmio en granos de cacaoCIAT
 
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoteras
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoterasTécnicas para disminuir la disponibilidad de cadmio en suelos de cacaoteras
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoterasCIAT
 
Cacao and Cadmium Research at Penn State
Cacao and Cadmium Research at Penn StateCacao and Cadmium Research at Penn State
Cacao and Cadmium Research at Penn StateCIAT
 
Aportes para el manejo de Cd en cacao
Aportes para el manejo de Cd en cacaoAportes para el manejo de Cd en cacao
Aportes para el manejo de Cd en cacaoCIAT
 
CENTRO DE INNOVACIÓN DEL CACAO PERÚ
CENTRO DE INNOVACIÓN DEL CACAO PERÚCENTRO DE INNOVACIÓN DEL CACAO PERÚ
CENTRO DE INNOVACIÓN DEL CACAO PERÚCIAT
 
Investigaciones sore Cadmio en el Cacao Colombiano
Investigaciones sore Cadmio en el Cacao ColombianoInvestigaciones sore Cadmio en el Cacao Colombiano
Investigaciones sore Cadmio en el Cacao ColombianoCIAT
 
Avances de investigación en cd en cacao
Avances de investigación en cd en cacaoAvances de investigación en cd en cacao
Avances de investigación en cd en cacaoCIAT
 

Plus de CIAT (20)

Agricultura Sostenible y Cambio Climático
Agricultura Sostenible y Cambio ClimáticoAgricultura Sostenible y Cambio Climático
Agricultura Sostenible y Cambio Climático
 
Resumen mesas trabajo
Resumen mesas trabajoResumen mesas trabajo
Resumen mesas trabajo
 
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...Impacto de las intervenciones agricolas y de salud para reducir la deficienci...
Impacto de las intervenciones agricolas y de salud para reducir la deficienci...
 
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...
Agricultura sensible a la nutrición en el Altiplano. Explorando las perspecti...
 
El rol de los padres en la nutrición del hogar
El rol de los padres en la nutrición del hogarEl rol de los padres en la nutrición del hogar
El rol de los padres en la nutrición del hogar
 
Scaling up soil carbon enhancement contributing to mitigate climate change
Scaling up soil carbon enhancement contributing to mitigate climate changeScaling up soil carbon enhancement contributing to mitigate climate change
Scaling up soil carbon enhancement contributing to mitigate climate change
 
Impacto del Cambio Climático en la Agricultura de República Dominicana
Impacto del Cambio Climático en la Agricultura de República DominicanaImpacto del Cambio Climático en la Agricultura de República Dominicana
Impacto del Cambio Climático en la Agricultura de República Dominicana
 
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...
BioTerra: Nuevo sistema de monitoreo de la biodiversidad en desarrollo por el...
 
Investigaciones sobre Cadmio en el Cacao Colombiano
Investigaciones sobre Cadmio en el Cacao ColombianoInvestigaciones sobre Cadmio en el Cacao Colombiano
Investigaciones sobre Cadmio en el Cacao Colombiano
 
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue in Colo...
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue    in Colo...Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue    in Colo...
Cacao for Peace Activities for Tackling the Cadmium in Cacao Issue in Colo...
 
Tackling cadmium in cacao and derived products – from farm to fork
Tackling cadmium in cacao and derived products – from farm to forkTackling cadmium in cacao and derived products – from farm to fork
Tackling cadmium in cacao and derived products – from farm to fork
 
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...
Cadmium bioaccumulation and gastric bioaccessibility in cacao: A field study ...
 
Geographical Information System Mapping for Optimized Cacao Production in Col...
Geographical Information System Mapping for Optimized Cacao Production in Col...Geographical Information System Mapping for Optimized Cacao Production in Col...
Geographical Information System Mapping for Optimized Cacao Production in Col...
 
Contenido de cadmio en granos de cacao
Contenido de cadmio en granos de cacaoContenido de cadmio en granos de cacao
Contenido de cadmio en granos de cacao
 
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoteras
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoterasTécnicas para disminuir la disponibilidad de cadmio en suelos de cacaoteras
Técnicas para disminuir la disponibilidad de cadmio en suelos de cacaoteras
 
Cacao and Cadmium Research at Penn State
Cacao and Cadmium Research at Penn StateCacao and Cadmium Research at Penn State
Cacao and Cadmium Research at Penn State
 
Aportes para el manejo de Cd en cacao
Aportes para el manejo de Cd en cacaoAportes para el manejo de Cd en cacao
Aportes para el manejo de Cd en cacao
 
CENTRO DE INNOVACIÓN DEL CACAO PERÚ
CENTRO DE INNOVACIÓN DEL CACAO PERÚCENTRO DE INNOVACIÓN DEL CACAO PERÚ
CENTRO DE INNOVACIÓN DEL CACAO PERÚ
 
Investigaciones sore Cadmio en el Cacao Colombiano
Investigaciones sore Cadmio en el Cacao ColombianoInvestigaciones sore Cadmio en el Cacao Colombiano
Investigaciones sore Cadmio en el Cacao Colombiano
 
Avances de investigación en cd en cacao
Avances de investigación en cd en cacaoAvances de investigación en cd en cacao
Avances de investigación en cd en cacao
 

Dernier

The State of Passkeys with FIDO Alliance.pptx
The State of Passkeys with FIDO Alliance.pptxThe State of Passkeys with FIDO Alliance.pptx
The State of Passkeys with FIDO Alliance.pptxLoriGlavin3
 
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyes
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyesHow to Effectively Monitor SD-WAN and SASE Environments with ThousandEyes
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyesThousandEyes
 
TrustArc Webinar - How to Build Consumer Trust Through Data Privacy
TrustArc Webinar - How to Build Consumer Trust Through Data PrivacyTrustArc Webinar - How to Build Consumer Trust Through Data Privacy
TrustArc Webinar - How to Build Consumer Trust Through Data PrivacyTrustArc
 
Generative Artificial Intelligence: How generative AI works.pdf
Generative Artificial Intelligence: How generative AI works.pdfGenerative Artificial Intelligence: How generative AI works.pdf
Generative Artificial Intelligence: How generative AI works.pdfIngrid Airi González
 
So einfach geht modernes Roaming fuer Notes und Nomad.pdf
So einfach geht modernes Roaming fuer Notes und Nomad.pdfSo einfach geht modernes Roaming fuer Notes und Nomad.pdf
So einfach geht modernes Roaming fuer Notes und Nomad.pdfpanagenda
 
Genislab builds better products and faster go-to-market with Lean project man...
Genislab builds better products and faster go-to-market with Lean project man...Genislab builds better products and faster go-to-market with Lean project man...
Genislab builds better products and faster go-to-market with Lean project man...Farhan Tariq
 
DevEX - reference for building teams, processes, and platforms
DevEX - reference for building teams, processes, and platformsDevEX - reference for building teams, processes, and platforms
DevEX - reference for building teams, processes, and platformsSergiu Bodiu
 
What is DBT - The Ultimate Data Build Tool.pdf
What is DBT - The Ultimate Data Build Tool.pdfWhat is DBT - The Ultimate Data Build Tool.pdf
What is DBT - The Ultimate Data Build Tool.pdfMounikaPolabathina
 
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...Scott Andery
 
Generative AI for Technical Writer or Information Developers
Generative AI for Technical Writer or Information DevelopersGenerative AI for Technical Writer or Information Developers
Generative AI for Technical Writer or Information DevelopersRaghuram Pandurangan
 
Take control of your SAP testing with UiPath Test Suite
Take control of your SAP testing with UiPath Test SuiteTake control of your SAP testing with UiPath Test Suite
Take control of your SAP testing with UiPath Test SuiteDianaGray10
 
UiPath Community: Communication Mining from Zero to Hero
UiPath Community: Communication Mining from Zero to HeroUiPath Community: Communication Mining from Zero to Hero
UiPath Community: Communication Mining from Zero to HeroUiPathCommunity
 
Sample pptx for embedding into website for demo
Sample pptx for embedding into website for demoSample pptx for embedding into website for demo
Sample pptx for embedding into website for demoHarshalMandlekar2
 
Testing tools and AI - ideas what to try with some tool examples
Testing tools and AI - ideas what to try with some tool examplesTesting tools and AI - ideas what to try with some tool examples
Testing tools and AI - ideas what to try with some tool examplesKari Kakkonen
 
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptx
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptxMerck Moving Beyond Passwords: FIDO Paris Seminar.pptx
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptxLoriGlavin3
 
Scale your database traffic with Read & Write split using MySQL Router
Scale your database traffic with Read & Write split using MySQL RouterScale your database traffic with Read & Write split using MySQL Router
Scale your database traffic with Read & Write split using MySQL RouterMydbops
 
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptx
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptxThe Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptx
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptxLoriGlavin3
 
Data governance with Unity Catalog Presentation
Data governance with Unity Catalog PresentationData governance with Unity Catalog Presentation
Data governance with Unity Catalog PresentationKnoldus Inc.
 
Connecting the Dots for Information Discovery.pdf
Connecting the Dots for Information Discovery.pdfConnecting the Dots for Information Discovery.pdf
Connecting the Dots for Information Discovery.pdfNeo4j
 
Digital Identity is Under Attack: FIDO Paris Seminar.pptx
Digital Identity is Under Attack: FIDO Paris Seminar.pptxDigital Identity is Under Attack: FIDO Paris Seminar.pptx
Digital Identity is Under Attack: FIDO Paris Seminar.pptxLoriGlavin3
 

Dernier (20)

The State of Passkeys with FIDO Alliance.pptx
The State of Passkeys with FIDO Alliance.pptxThe State of Passkeys with FIDO Alliance.pptx
The State of Passkeys with FIDO Alliance.pptx
 
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyes
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyesHow to Effectively Monitor SD-WAN and SASE Environments with ThousandEyes
How to Effectively Monitor SD-WAN and SASE Environments with ThousandEyes
 
TrustArc Webinar - How to Build Consumer Trust Through Data Privacy
TrustArc Webinar - How to Build Consumer Trust Through Data PrivacyTrustArc Webinar - How to Build Consumer Trust Through Data Privacy
TrustArc Webinar - How to Build Consumer Trust Through Data Privacy
 
Generative Artificial Intelligence: How generative AI works.pdf
Generative Artificial Intelligence: How generative AI works.pdfGenerative Artificial Intelligence: How generative AI works.pdf
Generative Artificial Intelligence: How generative AI works.pdf
 
So einfach geht modernes Roaming fuer Notes und Nomad.pdf
So einfach geht modernes Roaming fuer Notes und Nomad.pdfSo einfach geht modernes Roaming fuer Notes und Nomad.pdf
So einfach geht modernes Roaming fuer Notes und Nomad.pdf
 
Genislab builds better products and faster go-to-market with Lean project man...
Genislab builds better products and faster go-to-market with Lean project man...Genislab builds better products and faster go-to-market with Lean project man...
Genislab builds better products and faster go-to-market with Lean project man...
 
DevEX - reference for building teams, processes, and platforms
DevEX - reference for building teams, processes, and platformsDevEX - reference for building teams, processes, and platforms
DevEX - reference for building teams, processes, and platforms
 
What is DBT - The Ultimate Data Build Tool.pdf
What is DBT - The Ultimate Data Build Tool.pdfWhat is DBT - The Ultimate Data Build Tool.pdf
What is DBT - The Ultimate Data Build Tool.pdf
 
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...
Enhancing User Experience - Exploring the Latest Features of Tallyman Axis Lo...
 
Generative AI for Technical Writer or Information Developers
Generative AI for Technical Writer or Information DevelopersGenerative AI for Technical Writer or Information Developers
Generative AI for Technical Writer or Information Developers
 
Take control of your SAP testing with UiPath Test Suite
Take control of your SAP testing with UiPath Test SuiteTake control of your SAP testing with UiPath Test Suite
Take control of your SAP testing with UiPath Test Suite
 
UiPath Community: Communication Mining from Zero to Hero
UiPath Community: Communication Mining from Zero to HeroUiPath Community: Communication Mining from Zero to Hero
UiPath Community: Communication Mining from Zero to Hero
 
Sample pptx for embedding into website for demo
Sample pptx for embedding into website for demoSample pptx for embedding into website for demo
Sample pptx for embedding into website for demo
 
Testing tools and AI - ideas what to try with some tool examples
Testing tools and AI - ideas what to try with some tool examplesTesting tools and AI - ideas what to try with some tool examples
Testing tools and AI - ideas what to try with some tool examples
 
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptx
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptxMerck Moving Beyond Passwords: FIDO Paris Seminar.pptx
Merck Moving Beyond Passwords: FIDO Paris Seminar.pptx
 
Scale your database traffic with Read & Write split using MySQL Router
Scale your database traffic with Read & Write split using MySQL RouterScale your database traffic with Read & Write split using MySQL Router
Scale your database traffic with Read & Write split using MySQL Router
 
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptx
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptxThe Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptx
The Fit for Passkeys for Employee and Consumer Sign-ins: FIDO Paris Seminar.pptx
 
Data governance with Unity Catalog Presentation
Data governance with Unity Catalog PresentationData governance with Unity Catalog Presentation
Data governance with Unity Catalog Presentation
 
Connecting the Dots for Information Discovery.pdf
Connecting the Dots for Information Discovery.pdfConnecting the Dots for Information Discovery.pdf
Connecting the Dots for Information Discovery.pdf
 
Digital Identity is Under Attack: FIDO Paris Seminar.pptx
Digital Identity is Under Attack: FIDO Paris Seminar.pptxDigital Identity is Under Attack: FIDO Paris Seminar.pptx
Digital Identity is Under Attack: FIDO Paris Seminar.pptx
 

Spatial and Temporal Expression of a Citrate Permease and Aluminium-induced Citrate Exudation in Common Bean

  • 1. Spatial and Temporal Expression of a Citrate Permease and Aluminium-induced Citrate Exudation in Common Bean Dejene Eticha1, Andrés F. Rangel1,2, Idupulapati M. Rao3 and Walter J. Horst1 1Institute of Plant Nutrition, Leibniz University Hannover, Herrenhaeuser Str. 2, D-30419 Hannover, Germany; e-Mail: dejene.eticha@pflern.uni-hannover.de 2YARA GmbH & Co. KG, Hanninghof 35, D-48249 Dülmen, Germany 3International Center For Tropical Agriculture (CIAT) AA 6713, Cali, Colombia Introduction Aluminium (Al) toxicity is one of the major limiting factors for plant growth in acid soils especially in the tropics where common bean (Phaseolus vulgaris) is produced. Al resistance in common bean is conferred by citrate exudation which detoxifies Al in the root apoplast. Citrate exudation starts after a considerable lag period subsequent to Al treatment indicating that the activation of resistance mechanism involves Al-induced gene expression (Rangel et al., 2010). We identified a putative citrate permease gene PvMATE (Phaseolus vulgaris Multidrug and Toxin Extrusion) which is highly up regulated by Al treatment (Eticha et al., 2010). Here, the spatial and temporal expression of PvMATE in the root tip in relation to citrate exudation is presented. Materials and methods Two contrasting common bean genotypes, Quimbaya (Al-resistant) and VAX 1 (Al-sensitive) were used to study gene expression as well as citrate exudation of the different apical root zones. Results Root growth of both genotypes is equally inhibited in short-term Al treatment PvMATE is strongly expressed at the root tip compared to the elongation zone. This 1 2 correlates with the high citrate exudation at the root apex. Citrate exudation in the but genotypic difference is observed in medium- and long-term treatment. elongation zone is still substantially high indicating that in common bean, not only the transition but also the elongation zone must be protected. Relative expression level 4000 Relative root elongation (%) 100 Quimbaya (R) Control Vax-1 (S) Al [20 µM] 3000 80 PvMATE expression 2000 60 1000 0 40 100 [pmol (mm root)-1h-1] Citrate exudation rate 20 80 60 Citrate exudation 0 40 1 2 3 4 5 6 7 8 9 10 12 16 20 24 20 Al treatment time (h) 0 0-3 3-6 6-10 Distance from the root apex (mm) Fig. 1. Root growth of two contrasting common bean genotypes Fig. 2. Expression of citrate permease gene (PvMATE) and citrate exudation treated with Al for up to 24h. at different root zones of Al-resistant bean genotype Qimbaya. Al-induced expression of STOP1 transcription factor precedes the enhanced Citrate exudation began after a delay of about 4 h of Al treatment. It is preceded by 3 expression of PvMATE indicating that STOP1 regulates the PvMATE gene. 4 enhanced PvMATE expression. Citrate exudation continued to increase in the resistant genotype but dropped shortly afterwards in the sensitive genotype. 12 Relative expression level Quimbaya (R) 10 Vax-1 (S) 5 Quimbaya (R) [nmol (10-mm root tip)-1 h-1] 8 STOP1 VAX-1 (S) Citrate exudation rate 6 4 4 2 3 0 1000 Relative expression level 2 800 600 1 150 PvMATE 100 0 50 0 control 0-2 2-4 4-6 6-8 8-10 0 1 2 3 4 Al treatment time & citrate collection period (h) Al treatment time (h) Fig. 3. Gene expression of STOP1 and PvMATE at different Fig. 4. Citrate exudation of contrasting common bean genotypes at different durations of Al treatment. durations of Al treatment. Conclusion In common bean, citrate exudation involves a cascade of events which include the reception of an Al signal, activation of the STOP1 transcription factor, enhancement of citrate permease (pvMATE) gene expression and subsequent citrate exudation resulting in the maintenance of root growth under Al stress. Reference: Eticha et al. (2010) Annals of Botany, 105: 1119-1128. Rangel et al. (2010) Physiologia Plantarum, 38: 176-190. The financial support of the German Research Foundation (DFG) is highly acknowledged.