First Biology on SANS2d
•Télécharger en tant que PPTX, PDF•
2 j'aime•1,230 vues
Talk given at the Neutrons in Biology Meeting, held at Santa Fe, 24-29 October 2009
Recommandé
Contenu connexe
Similaire à First Biology on SANS2d
Similaire à First Biology on SANS2d (20)
Plus de Cameron Neylon
Plus de Cameron Neylon (20)
Dernier
Dernier (20)
First Biology on SANS2d
- 1. First Biology on SANS2D Will accelerator source time-of-flight SANS deliver for structural biology? Cameron Neylon
- 4. 4 Q sin( / 2 )
- 5. A range of wavelengths… http://www.flickr.com/photos/7519597@N05/2466444043
- 6. …to access more lengthscales http://www.flickr.com/photos/smithco/76749100
- 7. Wikimedia Commons Succinate_Dehydrogenase_1YQ3_and_Membrane.png Biology is complicated… Wikimedia Commons MGMT BDNA_1T38.png
- 8. …on a wide range… William Crochot/NIST Wikimedia Commons, used with permission
- 9. …of lengthscales Wikimedia Commons: Yeast_membrane_proteins.jpg
- 10. SANS is good for complex systems Wu et al., JBC, 2009, Manuscript M109.039537
- 11. So TOF SANS is great for biology? http://www.flickr.com/photos/thetruthabout/2842830409
- 12. But when we look for examples… http://www.flickr.com/photos/suzanneandsimon/1561911861
- 13. That’s an overstatement but…
- 14. Secretory IgA2 Bonner et al, J. BiolChem (2009) 284:5077 SANS data from LOQ
- 15. …few examples of TOF-SANS used in model building
- 21. The source http://www.flickr.com/photos/scobleizer/2442984975
- 23. …more neutrons
- 24. The instrument The instrument http://www.flickr.com/photos/cellonaut/3225137280
- 25. Collimation L1 = 2 to 12 m, Sample at 19m from moderator, Sample to detector L2 = 2 to 12 m, Qmin~ 0.002 Å-1, λ = 1.5 to 12 Å by time of flight, Qmax~ 3 Å-1 STFC
- 28. Adjustable collimation without windows STFC
- 33. Two detectors for wide Q range STFC
- 35. The data
- 36. Caveats Thick-walled moderator at low pressure Data from one detector only Instrument not fully aligned; collimation issues Issues with data reduction software slowing commissioning down
- 37. Lysozyme/D2O 8 mg.mL-1 3 hours L2 = 6m 6m collimation
- 41. Lysozyme/H2O
- 42. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1
- 43. GFP/D2O with aggregation Qmin ~ 0.005 Qmax ~ 0.4 Å-1 …one detector, one position
- 44. Problems… Issues with background subtraction Systematic errors in reduction process Instrument scattering artefacts Data reduction issues slowing optimization
- 45. Prospects… Enormous simultaneous Q range Two-fold flux increase from source Two-fold increase from alignment and collimation Three-fold from additional detector
- 46. Conclusions… Lots of work to identify instrument configurations A versatile instrument for hierarchical systems Competitive collection times wrtbest instruments Real potential for structural biology
- 48. Data from a mesostructured glass STFC Currently optimized Q-range of 0.02Å-1 to 50Å-1
- 49. 5Å 10Å 40Å 70Å STFC/Daniel Bowron 1Å-1 0.6Å-1 0.15Å-1 0.09Å-1
- 50. Prospects… World class SANS instrument for large samples High resolution data (but not verylow Q) Requires new data analysis techniques Combined data sets with differential labeling?
- 51. Reflectometers…
- 52. Reflectometers…
- 53. LMX ~2015
- 54. TS-2 and the biosciences Optimizedinstrumentation on a unique source Powerful tools for hierarchical systems Combining the best characteristics of instruments A view towards a future of long pulse sources?
- 57. Acknowledgements SANS2d: Richard Heenan, David Turner, James Treadgold, Sarah Rogers, Ann Terry & Steve King Sample preparation: Luke Clifton Data reduction: The MANTID team UK Large Facilities Capital Fund STFC