To save on precious antibodies and cells, CYTOO’s adhesive micropattern technology is also available in the smaller 10x10mm format: the CYTOOchips Mini. CYTOOchips Mini have a simplified single array layout and are available with standard or customized cell normalization micropatterns. They can be purchased coated in fibronectin (fluorescent or not) or as a Ready-to-Coat surface. Protocols are available to coat proteins such as collagen, PLL, laminin or Matrigel. The CYTOOchips Mini for Cell Normalization bear thousands of normalizing micropatterns which standardize cell shape and morphology down to their internal organization. Standard shapes include Disc, Crossbow, H, Y and L in three sizes.

1. chips Mini ™
Cell Normalization
Quantitative Immunofluorescence Analysis.
Delivered.

2. Choose the right micropattern for your application
chips Mini Cell Normalization For custom micropatterns please contact us contact@cytoo.com
Disc Crossbow H Y L
Quantitative Immunofluorescence Analysis. Delivered.
To save on precious antibodies and cells, CYTOO’s adhesive Adhesive patterns
micropattern technology is now available in the smaller 10x10mm
format: the CYTOOchips Mini.
CYTOOchips Mini have a simplified single array layout and are available
with standard or customized cell normalization micropatterns. Cells
They can be purchased coated in fibronectin (fluorescent or not) or as a
Ready-to-Coat surface. Coating protocols are provided for commonly used
Description No Strong Symmetric Triaxial symmetry Single free edge
proteins such as collagen, Poly-Lysine, laminin or Matrigel®.
polarization polarization organization organization organization
CYTOOchips Mini are not compatible with CYTOOchambers.
Noteworthy . Array cells . Polarize cells . Control symmetric . Study Multipolar . Measure contractility
Applications . Control cell spreading . Study internal cell division divisions and . Measure subtle alterations
supernumerary
Benefits and key features Applications and contraction
. Control ciliogenesis
organelle/endomembrane
spatial organisation
. Quantify cell-cell
adhesion/contacts centrosomes
in spindle orientation
during mitosis
. Control asymmetric ... ... ...
All the benefits of adhesive micropattern technology in a smaller format • Cell shape and actin cytoskeleton (contractility, actin ...
cell division
to save on precious reagents and cells: polymerization, …)
• Single cell isolation and arraying • Cell signaling events (protein translocation, signal
• Normalization of cell morphology and behavior transduction, …)
• Improved assay reproducibility and increased sensitivity • Cell polarity and organelle positioning Tip: Use CYTOOchips 20x20 Starter’s when getting started with Cell Normalization micropatterns to identify which
• Powerful quantification of cell phenotype: • Cell division and mitotic spindle orientation micropattern(s) work best for your specific application and cell line
• Control of the location of cell compartments and protein networks • Endomembrane network analysis
• Ideal for immunofluorescence, high resolution confocal microscopy
and AFM
• Stem cell differentiation A non exhaustive list of cell types already published on micropatterns
• Straightforward robust image analysis • Epithelial cells (HeLa, RPE-1, CHO, MDCK, BSC, MCF10A) • Neurons and neuron progenitors (SH-SY5Y; hippocampal
• Simple chip layout • Fibroblasts (murine NIH-3T3, BHK) and cortical neurons)
• Adenocarcinoma cell lines (MDA-231, A549) • Stem Cells (Human mesenchymal stem cells, mouse embryonic
• Hepatic cell lines (HepG2) stem cells/mESCs)
Normalized cells for quantitative Reference Cell™: powerful • Primary cells (Rat astrocytes, Rat ventricular myocytes,
wide-field and confocal microscopy quantification of cell phenotype myoblasts)
From as few as 50 normalized cells, a stack Check here for an updated list of cell types: www.cytoo.com/celltypes
of images can be converted into a single
representative image of the cell population
phenotype: the Reference Cell. Layout of CYTOOchips Mini
The Reference Cell is a unique methodology
allowing comparability between reproducible and Low autofluorescence optical
standardized assays. grade glass
Learn more at www.cytoo.com/referencecell Standard coverslip
170µm thickness (1.5)
Array of up to
6,800 identical patterns
Simple chip layout, 70 mm2
patterned area
Images
of individual
normalized Average image Fully adhesive area Laser engraved CYTOO
HeLa cells arrayed on CYTOOchips Mini (Crossbow Medium). cells (Actin) (Reference Cell) For side-by-side comparison of patterned For error-proof chip orientation.
The periphery of the chip offers a fully adhesive surface to allow
and non patterned cells No GPS
side by side comparison of normalized cells with their non
patterned counterparts (Image taken on a wide-field fluorescence
microscope, 10x) 1 cm

3. Product specifications
Substrate High quality low autofluorescence glass
Chip size 10 x 10 mm²
Glass thickness 170 μm (1.5)
Patterned area 7.8 x 8.7 mm²
Micropattern geometries Disc, Crossbow, H, Y, L and custom-made
Standard pattern sizes Small Medium Large
Nominal cell area 700 μm² 1,100 μm² 1,600 μm²
Rows x columns in the array 78 x 83 78 x 83 60 x 64
Number of micropatterns per chip 6,474 6,474 3,840
Pitch between patterns 100 μm 100 μm 130 μm
Micropattern line width 6 μm 8 μm 8 μm
Adhesion protein Fibronectin (FN), FN650*, or Activated (Ready-to-coat)**
Reference full adhesion area Yes
Error-free identification of patterned side Yes
Cell localization grid (block organization) No
Compatibility with CYTOOchambers No
Packaging By 12; in a blister pack sealed in aluminium bag under inert gas
* Fluorescent micropatterns (FN labeled with fluorophore with exc. =650 nm): useful for easier pattern identification, autofocusing as well as
for automated image analysis over multiple patterns.
** Activated CYTOOchips for adsorption of the protein of your choice (Collagen, Laminin, Poly-Lysine, Matrigel®, specific antibodies etc.). Protein may be
fluorescently labeled. Contact us for recommended coating protocols and specific needs.
Ordering information For inquiries please email sales@cytoo.com or contact us at www.cytoo.com/contact
Cat. No. Product Micropattern Size Packaging
11-001-X-12 CYTOOchips Mini DC-S-[Protein] Disc Small (700 µm²) Set of 12
11-002-X-12 CYTOOchips Mini DC-M-[Protein] Disc Medium (1100 µm²) Set of 12
11-003-X-12 CYTOOchips Mini DC-L-[Protein] Disc Large (1600 µm²) Set of 12
11-004-X-12 CYTOOchips Mini CW-S-[Protein] Crossbow Small (700 µm²) Set of 12
11-005-X-12 CYTOOchips Mini CW-M-[Protein] Crossbow Medium (1100 µm²) Set of 12
11-006-X-12 CYTOOchips Mini CW-L-[Protein] Crossbow Large (1600 µm²) Set of 12
11-007-X-12 CYTOOchips Mini H-S-[Protein] H Small (700 µm²) Set of 12
11-008-X-12 CYTOOchips Mini H-M-[Protein] H Medium (1100 µm²) Set of 12
11-009-X-12 CYTOOchips Mini H-L-[Protein] H Large (1600 µm²) Set of 12
11-010-X-12 CYTOOchips Mini Y-S-[Protein] Y Small (700 µm²) Set of 12
11-011-X-12 CYTOOchips Mini Y-M-[Protein] Y Medium (1100 µm²) Set of 12
11-012-X-12 CYTOOchips Mini Y-L-[Protein] Y Large (1600 µm²) Set of 12
11-013-X-12 CYTOOchips Mini L-S-[Protein] L Small (700 µm²) Set of 12
11-014-X-12 CYTOOchips Mini L-M-[Protein] L Medium (1100 µm²) Set of 12
11-015-X-12 CYTOOchips Mini L-L-[Protein] L Large (1600 µm²) Set of 12
CYTOO S.A.
7, parvis Louis Néel
Replace X by: Replace [Protein] by: BHT 52, BP50
• 00 – Activated ready-to-coat (no ECM protein); • A – Activated Ready-to-coat (no ECM protein); 38040 Grenoble cedex 9 -
• 10 – Fibronectin (FN) or • FN – Fibronectin or FRANCE
• 13 – Fluorescent FN with excitation at 650 nm • FN650 – Fluorescent FN with excitation at 650 nm
Tel. +33(0) 438 88 47 05
Further Reading CYTOO, Inc
Harvard Square
Find here a list of relevant publications: www.cytoo.com/publications One Mifflin Place
Suite 400
IP Rights Cambridge, MA 02138
Tel. +1 617 674 7711
Adhesive micropattern products provided by CYTOO are covered by European and US Patents and patents pending as well as their foreign equivalents.
These products may be covered by one or more Limited Use Label Licenses. The purchase of this product conveys to the buyer the non-transferable www.cytoo.com - info@cytoo.com
right to use the product in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise
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© 2009 CYTOO S.A.
transfer this product to a third party or otherwise use this product for Commercial Purposes. By the use of these products, you accept the terms and CYTOO™, CYTOOchips™, CYTOOplates™,
conditions of all applicable Limited Use Label Licenses. For research use only. Not intended for any animal or human therapeutic or diagnostic use. CYTOOchambers™ and Reference Cell™
are trademarks of CYTOO S.A.