Scaling API-first – The story of a global engineering organization
Phagos odonto
1. La Thérapie par les
Bactériophages
Approche par la Médecine Dentaire
BIF7000 – UQAM 2013
•Patricia Morales
•Natalia Samus
•Igor Lemos
•Deraldo Duran (Lisbon University)
5. BACKGROUND
Problématique
Dental caries
Periodontal diseases and dental caries are the
most prevalent chronic diseases, affecting
children, adolescents, adults and the elderly. In
fact, dental caries affects 60% to 90% of
schoolchildren and the vast majority of adults in
most industrialized countries.
http://www.phac-aspc.gc.ca/publicat/cdicmcbc/30-4/preface-eng.php#ref_05
9. BACKGROUND
Problématique
Efficacy of dental floss in
addition to a toothbrush
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X
In fact, dental caries affects 60% to 90% of
schoolchildren and the vast majority of adults in
most industrialized countries.
16. Lysin destroys a cell wall of grampositive bacteria (Streptococcus)
Lysin
Hydrolysis of (1->4)-beta-linkages
between N-acetylmuramic acid
and N-acetyl-D-glucosamine
residues in a peptidoglycan
Peptidoglycan
17. PHAST(PHAge Search Tool) - web server designed
to identify, annotate and graphically display prophage sequences
within bacterial genomes or plasmids
10947 Genomes Databases for download:
vIrus DB (57.902 Mb). Last update: Nov 1 2013
Prophage and virus DB (58.039 Mb). Last update: Nov 1 2013
Bacteria DB (2.626 Gb). Last update: Nov 1 2013
PHAST result DB (tar zip format) (104.167 Mb). Last update: Nov 1 2013
18. Pathologic Streptococci
(compete genom)
NC number
strain
PHAGE #
Str. mutans
NC_004350.1
UA159
1
Str. mutans
AP010655.1
NN2025
0
Str. mutans
CP003686.1
GS-5
0
Str. criceti
AEUV00000000
HS-6
-
Str. ratti
-
-
-
Str. sobrinus
-
-
-
Str. downei
AEKN00000000.1
F0415
1
Str. ferus
-
-
-
Str. macacae
AEUW00000000
NCTC 11558
-
Allan L. Delisle, Ming Guo, Natalia I. Chalmers, et al Appl Environ Microbiol. 2012 Apr;78(7):2264-71
29. Résultats
•En utilisant la base de données de PHAST nous avons trouvé
rapidement deux Streptococcus pathogènes (Str. mutants et
Str. downei) les quels contiennent gènes de phages dans ses
génomes.
•On a trouvé parmi ces deux bacteriophages specifiques de
streptococcus mutants (M-102 and PH-10), le gène qui
contiennent la protéine lysin, la plus active des protéines
antimicrobiennes.
•Ainsi les protéines de ces phages pourraient être concernés
comme des agents potentiels contre les streptocoques
pathogènes pour d'autres études.