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TL1
objective 3, workplan changes

          Bodo Raatz, CIAT
       Addis Ababa, May 2012



                                1
Thanks to..
•   DARS (Malawi)
•   SARI (Ethiopia)
•   DR&SS, (Zimbabwe)
•   KARI (Kenya)
•   ARI-Selian (Tanzania)
•   CIAT (Malawi, Uganda)




                            2
Activity 5, milestone 15:
  Inter-genepool advanced backcross
        population of 130 lines
• Andean X Meso BC populations BC2-F4
• Most lines badly adapted
• 2012: - few lines in breeding program
       - QTL appropach abandoned




                                          3
Activity 5.2: Advanced backcross




Andean recurrent parent CAL 96   Mesoamerican donor SER 16




                                                             4
MAGIC population in common bean
                                 (mesoamerican gene pool)
SXB 412           INB 827       ALB 213    SEN 56                   SCR 2       MIB 778            SCR 9           INB 841



          79 F1                     5 F1                                    38 F1                          99 F1


                       323 F1
                                                                                          272 F1

                                                      728 F1
                                                    500 selected

                                                      499 F2

                                                 996 F3 families



                                                996 F3.5 families



                                      F5:7 families for drought phenotyping         Genotype individuals

                                                                                                                      5
Activity 5, milestone 18-20:
      1 Mesoamerican multiple trait
      population phenotyped as F3.5
•   Decision to pursue MAGIC population
•   Genotyping in F5
•   Phenotyping F5:7
•   Currently in F4




                                          6
Activity 5: milestone 12
   MARS: Preliminary QTL analysis
                   performed
Sister lines SABs 626,       CAL 143
   628, 659, 686                – Released in 6
   – Drought             X        countries in southern
                                  Africa

  • 192 lines genotyped since last week
  • Results in analysis
  • Further genotyping required

                                                          7
Thank you for your attention!




                                8

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TLI 2012: Bean research workplan

  • 1. TL1 objective 3, workplan changes Bodo Raatz, CIAT Addis Ababa, May 2012 1
  • 2. Thanks to.. • DARS (Malawi) • SARI (Ethiopia) • DR&SS, (Zimbabwe) • KARI (Kenya) • ARI-Selian (Tanzania) • CIAT (Malawi, Uganda) 2
  • 3. Activity 5, milestone 15: Inter-genepool advanced backcross population of 130 lines • Andean X Meso BC populations BC2-F4 • Most lines badly adapted • 2012: - few lines in breeding program - QTL appropach abandoned 3
  • 4. Activity 5.2: Advanced backcross Andean recurrent parent CAL 96 Mesoamerican donor SER 16 4
  • 5. MAGIC population in common bean (mesoamerican gene pool) SXB 412 INB 827 ALB 213 SEN 56 SCR 2 MIB 778 SCR 9 INB 841 79 F1 5 F1 38 F1 99 F1 323 F1 272 F1 728 F1 500 selected 499 F2 996 F3 families 996 F3.5 families F5:7 families for drought phenotyping Genotype individuals 5
  • 6. Activity 5, milestone 18-20: 1 Mesoamerican multiple trait population phenotyped as F3.5 • Decision to pursue MAGIC population • Genotyping in F5 • Phenotyping F5:7 • Currently in F4 6
  • 7. Activity 5: milestone 12 MARS: Preliminary QTL analysis performed Sister lines SABs 626, CAL 143 628, 659, 686 – Released in 6 – Drought X countries in southern Africa • 192 lines genotyped since last week • Results in analysis • Further genotyping required 7
  • 8. Thank you for your attention! 8