1. LentiVectors, EpiVectors, TransVectors
Differences between the three cycles
First let’s see how a classic Lentiviral
vector works.
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2. LentiVectors
After fusion with the cell membrane, the
vector genome enters the cytoplasm.
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3. LentiVectors
The RNA vector genome is then transcribed
by the viral reverse-transcriptase (RT) into
linear DNA.
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4. LentiVectors
After translocation in the cell nucleus, the
DNA is integrated into the cell chromatin by
the HIV-1 integrase
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5. LentiVectors
Using the cell machinery, your protein of
interest is synthetized from the integrated
vector genome
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6. LentiVectors
With the integration of the vector genome,
the protein is assured to have a high-level
and long-term expression
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7. Now, let’s see how a non-integrating
lentiviral vector works
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8. EpiVectors
Beside integrating into the chromatin, the
DNA of viral origin can also form circles…
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9. EpiVectors
… which can be used as template for
transcription.
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10. If we disable the viral integrase…
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11. EpiVectors
The vector is no longer integrated but only
circularized…
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12. EpiVectors
… those circles are now the only template
used for your protein synthesis.
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13. EpiVectors
Due to the dilution effect of cell growth,
these circles can provide short-term
expression in dividing cells.
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14. EpiVectors
However in non-dividing cells, you will have a
long-term expression, yet lower than from
integrated vectors.
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15. If you want transient expression of your
transgene even in non-dividing cells,
TransVectors are what you need.
How does it work ?
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16. TransVectors
In a classic lentiviral vector, the reversetranscriptase binds to a specific region of the
vector genome called PBS
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17. TransVectors
In our TransVectors, the PBS region is deleted
from the vector genome.
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18. TransVectors
The binding of RT and reverse-transcription
are made impossible.
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19. TransVectors
And without reverse-transcription, the whole
cycle is impaired.
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20. TransVectors
Your protein synthesis now rely on a very
discreet process: translation directly from the
primary RNA vector genome
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21. TransVectors
Since RNAs have a short lifetime in the
cytoplasm, the protein expression will be
highly transient, even in non-dividing cells.
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22. LentiVectors, EpiVectors, TransVectors
Differences between the three cycles
The three lines of GEG-tech vectors provide
you with the opportunity to delimitate your
transgene expression in time.
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23. Viral genome
final state
LentiVectors
EpiVectors
TransVectors
Transgene
expression level
Integrated DNA
Transgene expression in:
Dividing cells
Non-dividing cells
High
Long term
Long term
Episomal DNA
Medium
Transient
Long term
RNA
Low
Transient
Transient
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