Vincent Denef: the impact of invasive species on freshwater microbial communities

1. Vincent Denef
Ecology and Evolutionary Biology, University of Michigan
Water @ Michigan
January 21, 2016
The difference a species makes:
impact of invasive species on freshwater microbial
communities
@VJDenef
vdenef@umich.edu

2. The difference a species makes
Texasinvasives.org
Invasion of the Dreissenids

3. [Credit: Nalepa, GLERL
Invasion of the Dreissenids
The difference a species makes

4. [Higgins, Ecol Mono, 2010]
Bacteria 0 ? ? ?
Invasion of the Dreissenids
The difference a species makes

5. Biota
Phytoplankton
Micro/nanozooplankton
Phototrophic
bacteria
Ecosystem
impacts
Chemotrophic
Bacteria (benthic-pelagic)
Microbial – higher
food web connectivity
Geochemical cycling
(e.g. CO2 efflux)
[Buffam et al, GCB, 2011]
July ‘99
July ‘10
[Credit: Vanderploeg, GLERL]
Chl a concentration
Impacts on bacterial communities
[Credit: Vanderploeg, GLERL]
Harmful algal blooms
(e.g., Microcystis)
The difference a species makes

6. Source water: Lake MI M45, 100 micron pre-filtered
Quagga mussels: Lake MI M45, cleaned and acclimated in lab for 12 hrs
20L
3 hrs
4 °C
20L
20L
20L
X 3
X 4
X 3
X 4
16S rRNA V4
sequencing
Tom Johengen Hank Vaderploeg
Experimental design
Feeding experiments

7. * = statistically significant difference (LEfSe, p < 0.05)
Impacts on bacterial communities: feeding experiment
Feeding experiments

8. Impacts on bacterial communities: field observations
Field observations

9. Impacts on bacterial communities: field observations
Field observations

10. Acknowledgments
NOAA GLERL
Ashley Baldridge
Joann Cavaletto
Jim Liebig
Ed Rutherford
Henry Vanderploeg
Crew of the R/V Laurentian
University of Michigan
Michelle Berry
Ashley Burtner
Edna Chiang *
Seana Florida *
Masanori Fujimoto ***
Thomas Johengen
Ann McCarthy
Ryan Keeling *
Morgan Meade *
Justin Millar
Tom Nalepa
Danna Palladino
Marian Schmidt **
Amelia Waters *
Amadeus Twu *
Support