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Who We Are?

An industrial biotech company situated in the CSIR Mining Innovation Centre,
with over 6 years already dedicated to R&D in conjunction with the Council for
Science and Industrial Research (CSIR) to develop sustainable biocides.

Based on the development of DECONT-X, a mild organic biocidal active ingredient,
BiODX products have gained popularity due to its non-corrosive properties and nontoxicity surrounding microbial decontamination.

Industrial Formulations
Food and Beverage
Health
Agricultural
Copyright BiODX 2013 All Rights Reserved
The Technology

• BiODX’s research was based upon a principal which assumed that triggering
an action on sensory parameters of citrus would yield high dosages of
biological yield from the target gene.

• BiODX’s innovative processes use the natural enzyme reaction occurring from
the time of picking the fruit, where degradation of the antimicrobial component
starts as the fruit converts all resources (sugars, bioflavonoids, antimicrobials
etc.) in order to stay alive.
• Innovation is also seen in the formation of two active compounds that do not
exist in the raw fruit; these two compounds are formed by virtue of the digestion
process that converts citric acid and vitamins.

• Formulating preservative into the juice is a stoichiometric reaction. With
reference to the formation of vitamin C, a citrus oxidation-reduction system has
electron donating and electron accepting properties.
Copyright BiODX 2013 All Rights Reserved
Active Citrus Extract

GC (gas chromatography) analysis identified active peak in citrus extract.

Bioflavonoids which is a main component of citrus has previously been reported
as having a growth inhibitory effect on bacteria such as colibacillus,
Staphylococcus aureus or methicillin-resistant Staphylococcus aureus.
Copyright BiODX 2013 All Rights Reserved
Stabilised Citrus Extract

GC (gas chromatography) analysis of DECONT-X showing activity compounds and
newly formed sub compounds post digestion.

The reaction between the citrus extract and QAC is a stoichiometric reaction
causing an ion exchange between the two compounds allowing the big molecule
(citrus extract) to digest certain sugars and other elements into newly formed
compounds.
Copyright BiODX 2013 All Rights Reserved
Regulatory Compliance

Originating in response to pollution laws in the USA, organic chemists use all the tools
and training of traditional chemistry to create non-hazardous chemicals and processes
that are "benign by design".
SABS SPECIFICATION
NUMBER

DESCRIPTION

FDA & EPA (United States of
America) Regulation

SANS 636

Disinfectants based on Quaternary
Ammonium Compounds

400ppm

SANS 1615

Detergent-Disinfectants based on
Glutaraldehyde

400ppm

SANS 1828

Cleaning Chemicals for the Food
Industry

400ppm

SANS 1853

Disinfectants and Detergent
Disinfectants for use in the Food
Industry

400ppm

SABS MARK

Quality Management System

ISO Accredited

KOSHER

Certification

Eco-Friendly Disinfectant

HALAAL

Pending

Copyright BiODX 2013 All Rights Reserved
Product Comparative
Safety Matrix
Organic chemistry describes a way of carrying out industrial activities from start (design)
to finish (production), it is driven by overwhelming consumer demands, regulatory and
economic factors.
Ingredient

Toxicity

Environmental
Concerns

Handling

Sensory
Effect

Corrosion

Natural

Alcohols

N

N

Y

N

N

N

Aldehydes

Y

N

N

N

N

N

Paracetic Acid

Y

N

Y

Y

Y

N

Phenols

Y

Y

N

Y

N

N

Hypochlorite

Y

Y

Y

N

Y

N

Hydrogen
Peroxide

N

N

Y

N

Y

N

DECONT-X

N

Genopole

N

N

N

N

Copyright BiODX 2013 All Rights Reserved
BiODX Products VS.
Conventional Chemicals

Bring innovation to your customers to have differentiation from competition.
Conventional

60% Natural

80% Natural

Chlorine,
Heavy Metals,
Solvents,
Paracetic Acid,
Caustic Soda,
Aldehydes

Pollution, effluent fines, corrosive,
toxic waste, kills enzymes,
damage to the environment

Earth Friendly, biodegradable,
non-corrosive, non-toxic,
harmless to the environment
Copyright BiODX 2013 All Rights Reserved
About the Product

Properties of DECONT-X:

• All South African
• Earth friendly and fully biodegradable.
• Bactericidal, fungicidal and virucidal.

• Pleasant characteristic fragrance due to citrus content.
• Has characteristic detergent properties.
• Unaffected by ammonia & nitrates.

• Not reactive with organic matter (no THM).
• Fast kill kinetics on both sessile and planktonic bacteria.
Copyright BiODX 2013 All Rights Reserved
Safety Characteristics

•

Non-irritant at normal use levels

•

Non-carcinogenic

SS

•

Non-mutagenic

•

Non-embryotoxic

•

Non-teratogenic

•

Contains no alcohol, formaldehydes or other harmful chemicals.

•

Chloride Free - does not generate any induced chemical corrosion regardless
the residence or contact time.
Copyright BiODX 2013 All Rights Reserved
Compatibility,
Solubility & Stability
•

DECONT-X is miscible with water and polar organic solvents.

•

Stable in acid and alkaline pH environments from pH 2 up to pH 11.

•

Compatible with a wide range of additives and auxiliary products including
surfactants, flocculants, dispersants, corrosion inhibitors and other biocides.

•

In common with other non-oxidising biocides, strong reducing and oxidising
agents should be avoided.

•

A potent antimicrobial with broad spectrum activity against Gram negative and

Gram positive bacteria, SRB’s, APB’s, anaerobes, yeasts and fungi.
•

Possible anion replacement for stabilization under acid condition.
Copyright BiODX 2013 All Rights Reserved
Product Performance

The “intelligence” of DECONT-X kills 99.9 % of pathogenic micro-organisms &
MIC’s (microbials influencing corrosion).
•

Escherichia coli sp.

•

Pseudomonas sp.

•

Staphylococcus sp. + Streptococcus sp.

•

Listeria sp.

•

Effective against legionella at as low as 30 ppm.

•

Black, green & brown moulds (Stachybotrys)

•

SRB’s, ARB’s & Anaerobes (MIC’s)

•

Remarkably effective against Pseudomonas, Stachybotrys and MIC’s.
Copyright BiODX 2013 All Rights Reserved
Performance
on Hard Surfaces

Microbial Count

The following results show the microbial control obtained at the beginning,
during and completion of a 3 month trial.

Copyright BiODX 2013 All Rights Reserved
DECONT-X Benchmarking VS.
Conventional Chemicals

Amount of Bacteria cfu/mL

The number of internal failures related to MIC and chemical corrosion issues
degrease by 80% with the use of DECONT-X.

Copyright BiODX 2013 All Rights Reserved
Comparative MIC Agent
Corrosion Rates
DECONT-X allows long residence time without generating any induced chemical
corrosion and offers a technical alternate to reduce dependency on Glut and THPS.
Autoclave Corrosion Results on 1018 Steel

MPY
Copyright BiODX 2013 All Rights Reserved
Markets for DECONT-X

•

Oil / Petroleum (fuel additive)

•

Fracking

•

Pulp / Paper Mill Industry

•

Water Treatment / Cooling Towers

•

Biofilm Control

•

Disinfectant Formulation

•

Preservation / Coatings / Paint

•

Printing Chemicals / Ink

BiODX have positioned DECONT-X selectively in these markets where a
sustainable biocide will have a competitive advantage due to end-user concerns
related to the environment, product performance and toxicity.
Copyright BiODX 2013 All Rights Reserved
Toxicity &
Corrosion Matrix
Conventional chemicals as demonstrated pose harmful risks to the labour force
promoting absenteeism and corrosive properties leads to equipment damage
resulting in huge financial losses.
Conventional
Chemicals
Max

Toxicity /
Corrosion

Sustainable
Chemicals
Paracetic Acid
Phenols
Hypochlorite
Hydrogen Peroxide
Aldehydes
Health /
Financial
Losses

Min
Natural

Natural
Copyright BiODX 2013 All Rights Reserved
Product Advantages

One of many comparative examples of sustainable VS. conventional chemicals
as applicable to cooling towers and there affect on water chemistry.
DECONT-X

CHLORINE

Unaffected by ammonia & nitrates

Affected by ammonia and nitrates

Non-toxic, no health & safety concerns

Major health & safety concerns

pH Neutral
Perceived as a protein/food - no resistance

Affects water pH
Bacterial strains can resist it

Non-corrosive

Highly corrosive

Outstandingly effective against biofilm

No efficacy against biofilm

No reaction to organic matter

Produces trihalomethanes / carcinogens

Stand alone product

Require extra chemicals

Stable at 5°c to 112°c

Unstable

At 15-25 ppm (in water) is cheaper than chlorine

Higher costs

Copyright BiODX 2013 All Rights Reserved
Total Bacteria Control in
Cooling Tower Water

Microbial Load (cfu/cm2)

The water sample was taken directly from a cooling tower that had undergone
standard treatment of a two ton shock treatment of chlorine added every two weeks.
The control did not get treated with any additional biocide during the 72 hours.

- Control
- DECONT-X

Time in Hours
Copyright BiODX 2013 All Rights Reserved
Bacterial Resistance

Bacteria learn to acquire immunity to conventional chemicals, which means:

• Stronger chemical concentrations
• Increased chemical costs
• Increased water / rinse costs

• Increased electricity / heating costs
• Increased process costs
• Increased labour / absenteeism costs
Bacteria do not identify DECONT-X as a poison and therefore do not imitate
development of resistance.
Copyright BiODX 2013 All Rights Reserved
The Knock-On Effect slide 1 of 2

Savings to a companies bottom-line are accrued by using sustainable chemicals, this
is the responsibility of the CEO as it influences many knock-on effects.

Product Cost

The Knock-on Effect

Conventional
Chemicals
Sustainable
Products

Water

Electricity

Effluent

Corrosion

Absenteeism

Sustainable Chemicals VS. Conventional Chemicals
Copyright BiODX 2013 All Rights Reserved
The Hidden Costs to the
Knock-On Effect
slide 2 of 2
Conventional chemicals require heat, rinsing, water chemistry and affect the pH,
this mixture of chemicals is harmful to the environment, human health and is
highly corrosive thus affecting the companies bottom-line!
Cost

Conventional

DECONT-X

Product Cost

€ 3.00

€ 7.50

Electricity

€ 0.40

€ 0.00

Water

€ 0.20

€ 0.00

Effluent

€ 1.00

€ 0.00

Stabilising Chemicals

€ 2.50

€ 0.00

Corrosion

€ 0.50

€ 0.00

Absenteeism

€ 2.00

€ 0.00

Total

€ 9.60

€ 7.50

Copyright BiODX 2013 All Rights Reserved
Are you Green or
Green-washed?

Become an authority!
Marketing green & overcoming green-washing.

Copyright BiODX 2013 All Rights Reserved
Drop the Sentiment!

Time for green sentiment to get out of the boardroom!

Bunnies go home!

Tree huggers out!

Green business is about making profits greener – with Industrial Ecology
Copyright BiODX 2013 All Rights Reserved
What are we really
selling?

An economy based on the intelligence of natural systems!

Copyright BiODX 2013 All Rights Reserved
Contact Us

Tel: 011 358-0052
info@biodx.co.za
www.biodx.co.za

CSIR – Mining Innovation Centre
Building 2
Cnr. Carlow & Rustenburg Rd
Melville, Johannesburg
South Africa
Graham Poultney
Tel: 011 358-0052
Cell: 082 447 3467
graham@biodx.co.za
www.biodx.co.za

Copyright BiODX 2013 All Rights Reserved

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BiODX - Antimicrobial Technology Presentation

  • 1.
  • 2. Who We Are? An industrial biotech company situated in the CSIR Mining Innovation Centre, with over 6 years already dedicated to R&D in conjunction with the Council for Science and Industrial Research (CSIR) to develop sustainable biocides. Based on the development of DECONT-X, a mild organic biocidal active ingredient, BiODX products have gained popularity due to its non-corrosive properties and nontoxicity surrounding microbial decontamination. Industrial Formulations Food and Beverage Health Agricultural Copyright BiODX 2013 All Rights Reserved
  • 3. The Technology • BiODX’s research was based upon a principal which assumed that triggering an action on sensory parameters of citrus would yield high dosages of biological yield from the target gene. • BiODX’s innovative processes use the natural enzyme reaction occurring from the time of picking the fruit, where degradation of the antimicrobial component starts as the fruit converts all resources (sugars, bioflavonoids, antimicrobials etc.) in order to stay alive. • Innovation is also seen in the formation of two active compounds that do not exist in the raw fruit; these two compounds are formed by virtue of the digestion process that converts citric acid and vitamins. • Formulating preservative into the juice is a stoichiometric reaction. With reference to the formation of vitamin C, a citrus oxidation-reduction system has electron donating and electron accepting properties. Copyright BiODX 2013 All Rights Reserved
  • 4. Active Citrus Extract GC (gas chromatography) analysis identified active peak in citrus extract. Bioflavonoids which is a main component of citrus has previously been reported as having a growth inhibitory effect on bacteria such as colibacillus, Staphylococcus aureus or methicillin-resistant Staphylococcus aureus. Copyright BiODX 2013 All Rights Reserved
  • 5. Stabilised Citrus Extract GC (gas chromatography) analysis of DECONT-X showing activity compounds and newly formed sub compounds post digestion. The reaction between the citrus extract and QAC is a stoichiometric reaction causing an ion exchange between the two compounds allowing the big molecule (citrus extract) to digest certain sugars and other elements into newly formed compounds. Copyright BiODX 2013 All Rights Reserved
  • 6. Regulatory Compliance Originating in response to pollution laws in the USA, organic chemists use all the tools and training of traditional chemistry to create non-hazardous chemicals and processes that are "benign by design". SABS SPECIFICATION NUMBER DESCRIPTION FDA & EPA (United States of America) Regulation SANS 636 Disinfectants based on Quaternary Ammonium Compounds 400ppm SANS 1615 Detergent-Disinfectants based on Glutaraldehyde 400ppm SANS 1828 Cleaning Chemicals for the Food Industry 400ppm SANS 1853 Disinfectants and Detergent Disinfectants for use in the Food Industry 400ppm SABS MARK Quality Management System ISO Accredited KOSHER Certification Eco-Friendly Disinfectant HALAAL Pending Copyright BiODX 2013 All Rights Reserved
  • 7. Product Comparative Safety Matrix Organic chemistry describes a way of carrying out industrial activities from start (design) to finish (production), it is driven by overwhelming consumer demands, regulatory and economic factors. Ingredient Toxicity Environmental Concerns Handling Sensory Effect Corrosion Natural Alcohols N N Y N N N Aldehydes Y N N N N N Paracetic Acid Y N Y Y Y N Phenols Y Y N Y N N Hypochlorite Y Y Y N Y N Hydrogen Peroxide N N Y N Y N DECONT-X N Genopole N N N N Copyright BiODX 2013 All Rights Reserved
  • 8. BiODX Products VS. Conventional Chemicals Bring innovation to your customers to have differentiation from competition. Conventional 60% Natural 80% Natural Chlorine, Heavy Metals, Solvents, Paracetic Acid, Caustic Soda, Aldehydes Pollution, effluent fines, corrosive, toxic waste, kills enzymes, damage to the environment Earth Friendly, biodegradable, non-corrosive, non-toxic, harmless to the environment Copyright BiODX 2013 All Rights Reserved
  • 9. About the Product Properties of DECONT-X: • All South African • Earth friendly and fully biodegradable. • Bactericidal, fungicidal and virucidal. • Pleasant characteristic fragrance due to citrus content. • Has characteristic detergent properties. • Unaffected by ammonia & nitrates. • Not reactive with organic matter (no THM). • Fast kill kinetics on both sessile and planktonic bacteria. Copyright BiODX 2013 All Rights Reserved
  • 10. Safety Characteristics • Non-irritant at normal use levels • Non-carcinogenic SS • Non-mutagenic • Non-embryotoxic • Non-teratogenic • Contains no alcohol, formaldehydes or other harmful chemicals. • Chloride Free - does not generate any induced chemical corrosion regardless the residence or contact time. Copyright BiODX 2013 All Rights Reserved
  • 11. Compatibility, Solubility & Stability • DECONT-X is miscible with water and polar organic solvents. • Stable in acid and alkaline pH environments from pH 2 up to pH 11. • Compatible with a wide range of additives and auxiliary products including surfactants, flocculants, dispersants, corrosion inhibitors and other biocides. • In common with other non-oxidising biocides, strong reducing and oxidising agents should be avoided. • A potent antimicrobial with broad spectrum activity against Gram negative and Gram positive bacteria, SRB’s, APB’s, anaerobes, yeasts and fungi. • Possible anion replacement for stabilization under acid condition. Copyright BiODX 2013 All Rights Reserved
  • 12. Product Performance The “intelligence” of DECONT-X kills 99.9 % of pathogenic micro-organisms & MIC’s (microbials influencing corrosion). • Escherichia coli sp. • Pseudomonas sp. • Staphylococcus sp. + Streptococcus sp. • Listeria sp. • Effective against legionella at as low as 30 ppm. • Black, green & brown moulds (Stachybotrys) • SRB’s, ARB’s & Anaerobes (MIC’s) • Remarkably effective against Pseudomonas, Stachybotrys and MIC’s. Copyright BiODX 2013 All Rights Reserved
  • 13. Performance on Hard Surfaces Microbial Count The following results show the microbial control obtained at the beginning, during and completion of a 3 month trial. Copyright BiODX 2013 All Rights Reserved
  • 14. DECONT-X Benchmarking VS. Conventional Chemicals Amount of Bacteria cfu/mL The number of internal failures related to MIC and chemical corrosion issues degrease by 80% with the use of DECONT-X. Copyright BiODX 2013 All Rights Reserved
  • 15. Comparative MIC Agent Corrosion Rates DECONT-X allows long residence time without generating any induced chemical corrosion and offers a technical alternate to reduce dependency on Glut and THPS. Autoclave Corrosion Results on 1018 Steel MPY Copyright BiODX 2013 All Rights Reserved
  • 16. Markets for DECONT-X • Oil / Petroleum (fuel additive) • Fracking • Pulp / Paper Mill Industry • Water Treatment / Cooling Towers • Biofilm Control • Disinfectant Formulation • Preservation / Coatings / Paint • Printing Chemicals / Ink BiODX have positioned DECONT-X selectively in these markets where a sustainable biocide will have a competitive advantage due to end-user concerns related to the environment, product performance and toxicity. Copyright BiODX 2013 All Rights Reserved
  • 17. Toxicity & Corrosion Matrix Conventional chemicals as demonstrated pose harmful risks to the labour force promoting absenteeism and corrosive properties leads to equipment damage resulting in huge financial losses. Conventional Chemicals Max Toxicity / Corrosion Sustainable Chemicals Paracetic Acid Phenols Hypochlorite Hydrogen Peroxide Aldehydes Health / Financial Losses Min Natural Natural Copyright BiODX 2013 All Rights Reserved
  • 18. Product Advantages One of many comparative examples of sustainable VS. conventional chemicals as applicable to cooling towers and there affect on water chemistry. DECONT-X CHLORINE Unaffected by ammonia & nitrates Affected by ammonia and nitrates Non-toxic, no health & safety concerns Major health & safety concerns pH Neutral Perceived as a protein/food - no resistance Affects water pH Bacterial strains can resist it Non-corrosive Highly corrosive Outstandingly effective against biofilm No efficacy against biofilm No reaction to organic matter Produces trihalomethanes / carcinogens Stand alone product Require extra chemicals Stable at 5°c to 112°c Unstable At 15-25 ppm (in water) is cheaper than chlorine Higher costs Copyright BiODX 2013 All Rights Reserved
  • 19. Total Bacteria Control in Cooling Tower Water Microbial Load (cfu/cm2) The water sample was taken directly from a cooling tower that had undergone standard treatment of a two ton shock treatment of chlorine added every two weeks. The control did not get treated with any additional biocide during the 72 hours. - Control - DECONT-X Time in Hours Copyright BiODX 2013 All Rights Reserved
  • 20. Bacterial Resistance Bacteria learn to acquire immunity to conventional chemicals, which means: • Stronger chemical concentrations • Increased chemical costs • Increased water / rinse costs • Increased electricity / heating costs • Increased process costs • Increased labour / absenteeism costs Bacteria do not identify DECONT-X as a poison and therefore do not imitate development of resistance. Copyright BiODX 2013 All Rights Reserved
  • 21. The Knock-On Effect slide 1 of 2 Savings to a companies bottom-line are accrued by using sustainable chemicals, this is the responsibility of the CEO as it influences many knock-on effects. Product Cost The Knock-on Effect Conventional Chemicals Sustainable Products Water Electricity Effluent Corrosion Absenteeism Sustainable Chemicals VS. Conventional Chemicals Copyright BiODX 2013 All Rights Reserved
  • 22. The Hidden Costs to the Knock-On Effect slide 2 of 2 Conventional chemicals require heat, rinsing, water chemistry and affect the pH, this mixture of chemicals is harmful to the environment, human health and is highly corrosive thus affecting the companies bottom-line! Cost Conventional DECONT-X Product Cost € 3.00 € 7.50 Electricity € 0.40 € 0.00 Water € 0.20 € 0.00 Effluent € 1.00 € 0.00 Stabilising Chemicals € 2.50 € 0.00 Corrosion € 0.50 € 0.00 Absenteeism € 2.00 € 0.00 Total € 9.60 € 7.50 Copyright BiODX 2013 All Rights Reserved
  • 23. Are you Green or Green-washed? Become an authority! Marketing green & overcoming green-washing. Copyright BiODX 2013 All Rights Reserved
  • 24. Drop the Sentiment! Time for green sentiment to get out of the boardroom! Bunnies go home! Tree huggers out! Green business is about making profits greener – with Industrial Ecology Copyright BiODX 2013 All Rights Reserved
  • 25. What are we really selling? An economy based on the intelligence of natural systems! Copyright BiODX 2013 All Rights Reserved
  • 26. Contact Us Tel: 011 358-0052 info@biodx.co.za www.biodx.co.za CSIR – Mining Innovation Centre Building 2 Cnr. Carlow & Rustenburg Rd Melville, Johannesburg South Africa Graham Poultney Tel: 011 358-0052 Cell: 082 447 3467 graham@biodx.co.za www.biodx.co.za Copyright BiODX 2013 All Rights Reserved