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1
 Introduction
› Importance
› Worldwide data
› Objectives
 Materials and methods
 Results and discussion
 Summary
 Conclusion
2
 Chemo-heterotrophic
 Decomposers in natural ecosystem
 Mushrooms
› Technically belong to kingdom fungi and phylum
Basidiomycota with gills, but
› largely fleshy woody fungi, bracket fungi belong to two
orders Agaricales and Aphyllophorales respectively
3
 Used as delicacy
 Potential protein source
 High content of essential amino acid, vitamins,
thiamin, riboflavin, niacin, minerals and low lipid
content
 Used in Medicinal tonics, various cosmetics
beverages, pharmaceutical industries
 Have capacity to convert nutritionally valueless
substances into high protein food
 Essential part of natural cycle of birth, death, decay
and renewal in all forest ecosystems
4
 115,000 of mushroom species known world-wide
 Agaricales 80,000 (fleshy fungi with cap & tail)
 Gasteromycetes 10,000 (puff balls & stink horn)
 Aphyllophorales 20,000 (toothed, Polypores)
 Jelly fungi 5000 i.e. Tremellales, Auriculariales
and Dacrymycetales (Hawksworth, 2001)
5
 To check the diversity of mushrooms in kuldanna
(Murree) forest
 To classify the fungi on the basis of their
distinguishing characteristics
 To assess the relationship between mushroom
species, occurrence, environmental factors and
vegetation type in Kuldanna forest of Murree
6
 From the whole collection sixteen samples of
mushrooms enlisted systematically with;
› Salient characters of genus and species
› Auto-ecological notes
› Habitat
› Distribution
› Medicinal value
› Edibility
7
COLLECTION SITE:
› Forest of Murree Kuldanna, Rawalpindi
› 2 Km square area selected
8
EXPERIMENTAL DESIGN
› Collection of samples with host from the cited
area after snapshot
› Drying of the specimen
› After identification placed in separate
envelope with all data
› Indentify on the basis of morphological
characters and microscopic characters
› Recorded observation also sketched on a
separate sheet of paper then matched them
with standard size i-e; compare it with existing
literature
› Placed in collection box with phenyl balls in
order to preserve from insect attack
9
Pleurotus ostreatus
 Importance
› Used industrially for
Myco-remediation purposes
› Delicious edible mushroom
› Lovastatin and Lysine
› Treatment of lumber and neck pain, numbness,
tumor suppression
10
 Very successful wood decaying fungus
 Beta glucan extracted, capable of relieving dry
condition, atopic
diseases and itching
inhibiting tumor
 If spores enter in blood
circulation causes
paralysis
11
 Voracious decomposer of dead wood
 Causes wood rot in standing trees
 Best producer of manganese peroxide
 Antibacterial, antifungal, and antitumor
12
 Important decayer of conifer wood
 Generally describe as saprophyte
 In some areas known to attack living trees
 Expelling wind, moisture
› Antitumor
13
 Saprotroph
 Degrade glucan along with bit of cellulose
 Contain unique compound including a metabolite,
concentricol (oxidized squallene)
 Tinder for fire lighting, like charcoal
 Home of insect and
small animals
14
 Used in traditional herbal remedies
 Two substances extracted from (PSK) &(PSP) being
studied as possible complementary cancer
treatment
 VPS, another extract sold as dietary supplement
 Decomposer
15
 On logs of deciduous trees
 Contain polysaccharide (anti-tumour)
 Immune system enhancing activity
16
 Edible
 Form symbiotic relationship with various species of
deciduous trees
 Treatment of backache leg pain, numbness, tumor
suppression
17
 Edible , but not recommended due to its taste
 Tumor suppression
18
 Decayer of plant material
 Present on wood and on mulchs grasses, wheat and
straws
 Anti-tumor
19
 Wood rotting saprobes
20
 Saprotroph
 Wood decayer causes “white rot” or “brown rot”
21
 Parasitic on deciduous trees
 Cause intensive white rot
 Tumor suppression
22
 Mature with red brown gills
 Poisonous
 Referred as wood woolly foot
23
 Present on trunk of living broad leaved trees
 Tonic herbal in traditional Chinese medicine
 Powerful antibiotic
 Liver tonic
 Immune tonic
 Lowering blood pressure
 Suppressing tumor
24
 On ground among Cynodon dactylon
 Edible
25
 Decayer
 Common on dead logs and dead woods
26
 Edible
 Treatment for gastrointestinal, Indigestion
 Extract, interferon anticancer
 Exportable
27
 MEDICINAL IMPORTANT FUNGI
1. Morchella spp
2. Pleurotus ostreatus
3. Pleurotus variety
4. Ganoderma lucidum
5. Lacterius piperatus
6. Lactarius pergamenus
7. Phellinus igniarius
8. Volvariella spp
9. Coriolus versicolor
10. Fomitopsis pinicola
11. Trichamptum biforme
12. Schizophyllum commune
13. Pleurotus ostreatus
28
1. Pleurotus ostreatus
2. Pleurotus variety
3. Lactarius piperatus
4. Lactarius pergamenus
5. Morchella spp
29
1. Coriolus hirsutus
2. Coriolus versicolor
3. Ganoderma lucidum
4. Hygrocybe
5. Lactarius pergamenus
6. Lactarius piperatus
7. Pleurotus ostreatus
8. Collybia peronata
9. Phellinus igniarius
10. Pluteus
11. Polyporus
12. Poria paradoxa
13. Schizophyllum commune
14. Volvariella
15. Trichamptum biforme
16. Fomitopsis pinicola 30
1. Coriolus hirsutus
2. Coriolus Versicolor
3. Ganoderma lucidum
4. Phellinus igniarius
5. Fomes
6. Pleurotus spp.
7. Poria
8. Schizophyllum
9. Polyporus
31
1. Lactarius piperatus
2. Lactarius pergamenus
3. Volvariella
32
Sr. No. Mode of living No. of sample percentage
1. Saprophytic 16/16 100%
2. Micorrhizal 9/16 56.25%
3. Parasitic 3/16 18.75%
33
34
Percentage of different living modes
of fungi
0
20
40
60
80
100
120
Saprophytic Parasitic Micorhizal
Different living modes of fungi
Percentage
Sr.No. Characteristics No. of sample Calculated
value of Chi
Tabulated
value
1. Medicinal 13/16 6.22 3.84
2. Non medicinal 3/16
3. Edible 5/16 2.24 3.84
4. Non edible 11/16
35
 This report would be beneficial to them who want
to study the mushroom taxonomically or
ecologically, biologist, chemist, biochemist and
industrialist will come to know about the mushroom
flora of this area
 They can arrange the required material to extract
the value able compound like anti tumor, anti
bacterial medicines
36
THANKS
37

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Oral presentation2003

  • 1. 1
  • 2.  Introduction › Importance › Worldwide data › Objectives  Materials and methods  Results and discussion  Summary  Conclusion 2
  • 3.  Chemo-heterotrophic  Decomposers in natural ecosystem  Mushrooms › Technically belong to kingdom fungi and phylum Basidiomycota with gills, but › largely fleshy woody fungi, bracket fungi belong to two orders Agaricales and Aphyllophorales respectively 3
  • 4.  Used as delicacy  Potential protein source  High content of essential amino acid, vitamins, thiamin, riboflavin, niacin, minerals and low lipid content  Used in Medicinal tonics, various cosmetics beverages, pharmaceutical industries  Have capacity to convert nutritionally valueless substances into high protein food  Essential part of natural cycle of birth, death, decay and renewal in all forest ecosystems 4
  • 5.  115,000 of mushroom species known world-wide  Agaricales 80,000 (fleshy fungi with cap & tail)  Gasteromycetes 10,000 (puff balls & stink horn)  Aphyllophorales 20,000 (toothed, Polypores)  Jelly fungi 5000 i.e. Tremellales, Auriculariales and Dacrymycetales (Hawksworth, 2001) 5
  • 6.  To check the diversity of mushrooms in kuldanna (Murree) forest  To classify the fungi on the basis of their distinguishing characteristics  To assess the relationship between mushroom species, occurrence, environmental factors and vegetation type in Kuldanna forest of Murree 6
  • 7.  From the whole collection sixteen samples of mushrooms enlisted systematically with; › Salient characters of genus and species › Auto-ecological notes › Habitat › Distribution › Medicinal value › Edibility 7
  • 8. COLLECTION SITE: › Forest of Murree Kuldanna, Rawalpindi › 2 Km square area selected 8
  • 9. EXPERIMENTAL DESIGN › Collection of samples with host from the cited area after snapshot › Drying of the specimen › After identification placed in separate envelope with all data › Indentify on the basis of morphological characters and microscopic characters › Recorded observation also sketched on a separate sheet of paper then matched them with standard size i-e; compare it with existing literature › Placed in collection box with phenyl balls in order to preserve from insect attack 9
  • 10. Pleurotus ostreatus  Importance › Used industrially for Myco-remediation purposes › Delicious edible mushroom › Lovastatin and Lysine › Treatment of lumber and neck pain, numbness, tumor suppression 10
  • 11.  Very successful wood decaying fungus  Beta glucan extracted, capable of relieving dry condition, atopic diseases and itching inhibiting tumor  If spores enter in blood circulation causes paralysis 11
  • 12.  Voracious decomposer of dead wood  Causes wood rot in standing trees  Best producer of manganese peroxide  Antibacterial, antifungal, and antitumor 12
  • 13.  Important decayer of conifer wood  Generally describe as saprophyte  In some areas known to attack living trees  Expelling wind, moisture › Antitumor 13
  • 14.  Saprotroph  Degrade glucan along with bit of cellulose  Contain unique compound including a metabolite, concentricol (oxidized squallene)  Tinder for fire lighting, like charcoal  Home of insect and small animals 14
  • 15.  Used in traditional herbal remedies  Two substances extracted from (PSK) &(PSP) being studied as possible complementary cancer treatment  VPS, another extract sold as dietary supplement  Decomposer 15
  • 16.  On logs of deciduous trees  Contain polysaccharide (anti-tumour)  Immune system enhancing activity 16
  • 17.  Edible  Form symbiotic relationship with various species of deciduous trees  Treatment of backache leg pain, numbness, tumor suppression 17
  • 18.  Edible , but not recommended due to its taste  Tumor suppression 18
  • 19.  Decayer of plant material  Present on wood and on mulchs grasses, wheat and straws  Anti-tumor 19
  • 20.  Wood rotting saprobes 20
  • 21.  Saprotroph  Wood decayer causes “white rot” or “brown rot” 21
  • 22.  Parasitic on deciduous trees  Cause intensive white rot  Tumor suppression 22
  • 23.  Mature with red brown gills  Poisonous  Referred as wood woolly foot 23
  • 24.  Present on trunk of living broad leaved trees  Tonic herbal in traditional Chinese medicine  Powerful antibiotic  Liver tonic  Immune tonic  Lowering blood pressure  Suppressing tumor 24
  • 25.  On ground among Cynodon dactylon  Edible 25
  • 26.  Decayer  Common on dead logs and dead woods 26
  • 27.  Edible  Treatment for gastrointestinal, Indigestion  Extract, interferon anticancer  Exportable 27
  • 28.  MEDICINAL IMPORTANT FUNGI 1. Morchella spp 2. Pleurotus ostreatus 3. Pleurotus variety 4. Ganoderma lucidum 5. Lacterius piperatus 6. Lactarius pergamenus 7. Phellinus igniarius 8. Volvariella spp 9. Coriolus versicolor 10. Fomitopsis pinicola 11. Trichamptum biforme 12. Schizophyllum commune 13. Pleurotus ostreatus 28
  • 29. 1. Pleurotus ostreatus 2. Pleurotus variety 3. Lactarius piperatus 4. Lactarius pergamenus 5. Morchella spp 29
  • 30. 1. Coriolus hirsutus 2. Coriolus versicolor 3. Ganoderma lucidum 4. Hygrocybe 5. Lactarius pergamenus 6. Lactarius piperatus 7. Pleurotus ostreatus 8. Collybia peronata 9. Phellinus igniarius 10. Pluteus 11. Polyporus 12. Poria paradoxa 13. Schizophyllum commune 14. Volvariella 15. Trichamptum biforme 16. Fomitopsis pinicola 30
  • 31. 1. Coriolus hirsutus 2. Coriolus Versicolor 3. Ganoderma lucidum 4. Phellinus igniarius 5. Fomes 6. Pleurotus spp. 7. Poria 8. Schizophyllum 9. Polyporus 31
  • 32. 1. Lactarius piperatus 2. Lactarius pergamenus 3. Volvariella 32
  • 33. Sr. No. Mode of living No. of sample percentage 1. Saprophytic 16/16 100% 2. Micorrhizal 9/16 56.25% 3. Parasitic 3/16 18.75% 33
  • 34. 34 Percentage of different living modes of fungi 0 20 40 60 80 100 120 Saprophytic Parasitic Micorhizal Different living modes of fungi Percentage
  • 35. Sr.No. Characteristics No. of sample Calculated value of Chi Tabulated value 1. Medicinal 13/16 6.22 3.84 2. Non medicinal 3/16 3. Edible 5/16 2.24 3.84 4. Non edible 11/16 35
  • 36.  This report would be beneficial to them who want to study the mushroom taxonomically or ecologically, biologist, chemist, biochemist and industrialist will come to know about the mushroom flora of this area  They can arrange the required material to extract the value able compound like anti tumor, anti bacterial medicines 36