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Welcome to the macroworld of microbiology 13 2
1. Second year Lab Medicine (Females)
1433- 1434 A.H. / 2012- 2013
Microbiology Practical (Course I)
Welcome to the Macroworld
of Microbiology
:By
Mrs. Hibah Abdul-Wahab I.
Abu-Sulaiman
2. What are the life forms?
Humans.
Animals.
Plants.
– And…
60. Aims of following safety rules and
practices
• To prevent the occurrence of accidental
infections in the lab and reduce them if
they occur.
• Prevent potentially harmful microbes from
leaving the lab.
61. Safety in the Microbiology Lab
• Wash your hands with
soap and water
before you begin and
after you finish your
work and before
leaving the lab.
62. Safety in the Microbiology Lab
• Decontaminate your
work surface before
and after you work
63. Safety in the Microbiology Lab
• No bags inside the
lab.
64. Safety in the Microbiology Lab
• Wear only closed-
toed shoes in the
laboratory.
65. Safety in the Microbiology Lab
• Wear lab scrub and
fully- buttoned lab
coat.
66. Safety in the Microbiology Lab
• Tight your hair.
• No cosmetics
67. Safety in the Microbiology Lab
• If you are:
– Injured…
– Immunocompromised..
– Pregnant…
– Allergic to latix…
68. Safety in the Microbiology Lab
• Be careful not to burn
yourself when using
Bunsen burner.
69. Safety in the Microbiology Lab
• Keep your hands
away from your
mouth and eyes…
70. Safety in the Microbiology Lab
• Do not eat, drink or
chew gum while you
are in the lab
71. Safety in the Microbiology Lab
• Avoid using electronic
devices …
– Mobiles, MP3 players,
laptops…
72. Safety in the Microbiology Lab
• Never mouth
pipetting.
73. Safety in the Microbiology Lab
• Always keep cultures
in a test tube rack
whether you are
working at your bench
or walking about in
the lab.
74. Safety in the Microbiology Lab
• Place all contaminated
tubes, plates and waste
materials in appropriate
receptacles for
subsequent sterilization.
75. Safety in the Microbiology Lab
• If you spill a chemical on
your skin, immediately
wash the affected area.
If a chemical or a culture
gets into your eye,
immediately wash your
eye for 5 min.
76. Safety in the Microbiology Lab
• If anything containing viable bacteria
spills, cover the spell or broken glass with
a paper towel and a generous amount of
disinfectant to remain on the slip for at
least 20 min. before cleaning it up.
77. Safety in the Microbiology Lab
• Sweep up pieces of broken glass (after
disinfection if necessary) and place them
in the specified container.
78. Safety in the Microbiology Lab
• Place sharps, such as
syringes and slides, in
a designated sharp
containers.
79. Safety in the Microbiology Lab
• Do not keep cultures in your lab drawer or
locker when you have finished with them.
• Do not remove cultures, reagents, or other
materials from the lab unless you have
permission to do so.
80. Safety in the Microbiology Lab
• Know the first aid kit location in your lab.