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October 23, 2013

Ask a Biosafety Expert:
User-driven advisory
service for the Do-It-Yourself
Biology (DIYbio) Community
ABSA Annual Meeting
Kansas City, MO

Jason Bobe
Co-founder, DIYbio.org
jason@diybio.org
twitter:@jasonbobe

1
New Teen Hobby: DIY DNA labs

Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
New Teen Hobby: DIY DNA labs

Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
Dawn of the Biohackers. Discover Magazine. Oct 5, 2011.
Photo by Grant Delin
Vibrant Hobby Communities
CHEMISTRY

Lab Technician Set for Girls
c. 1958

PERSONAL
COMPUTING

Homebrew Computer Club
September 1976

ASTRONOMY

Hanny’s Voorwerp discovered
August 13, 2007

ROCKETRY

Amateur Saturn V launch in MD
April 25, 2009

Credits: Chemical Heritage Foundation; DigiBarn Computer Museum; Sloan Digital Sky Survey; Dick Stafford, RocketryPlanet.com.
Biology is Next:
Global DIYbio Community
NORTH AMERICA
Atlanta
Baltimore
Boston
Brooklyn
Cambridge
Carlsbad
Chicago
Houston
Los Angeles
Nashville
New York City
Oakland
Portland
San Diego
San Francisco
Seattle
Sunnyvale
Toronto
Vancouver
Victoria

EUROPE
Budapest
Copenhagen
Cork
Eindhoven
Graz
Groningen
Kiev
Lausanne
London
Manchester
Munich
Namur
The Hague
Paris
Prague
Switzerland

ASIA
Singapore
Tel-Aviv
OCEANIA
Auckland
Sydney
Scope of Activities
Exploratory Biology

Environmental
Sensing

Personal
BioMonitoring

Constructive Biology

Re-imagining
Laboratory Devices

Genetic
Engineering

7
Home Workshops
1.Garage: cancer
biotech start-up
2.Closet: Personal
genotyping facility
3.Kitchen: yogurt
engineered to sense
melamine (toxin)
4.Dorm: R&D lab for
low-cost PCR machine
Community Labs
Genspace (Brooklyn, NY)

NYTimes. December 19, 2010. Photo Michael Nagle
Community Labs
BioCurious (Sunnyvale, CA)

http://biocuriosity.files.wordpress.com/2011/07/welcome-to-biocurious-sign.jpg?w=630
http://biocuriosity.files.wordpress.com/2011/09/img_0551.jpg?w=630
DIY BioPrinter

Biocurious Project: BioPrinter
6/ 2/ 13 2:50 PM

(/files/deriv/FK7/UUYW/HAQ3D3X1/FK7UUYWHAQ3D3X1.LARGE.jpg)
http://www.instructables.com/id/DIY-BioPrinter/

(/files/deriv/F8C/V1Y8/HBXWRTDL/F8CV1Y8HBXWRTDL.LARGE.jpg)

(/files/deriv/F7P/LTPM/HAQ3D3Z2/F7PLTPMHAQ3D3Z2.LARGE.jpg)

http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p
http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p
ublic/images/bioprinter_0.jpg?itok=ZkO-8Tay
ublic/images/biocurious.jpg?itok=CAGwqHG9
Innovation Engine: Small Start-Ups Creating
Low-Cost Kits & Devices --> Crowdsourcing $$$

Open GelBox 2.0

Microbial Fuel Cell

Dremelfuge

OpenPCR

LavaAmp
iGEM: International Genetically
Engineered Machines Competition
• 3200 active members
• 215 teams & 200 labs
• Sections
http://2012.igem.org/wiki/images/6/65/Biobrick_trophy.jpg

http://www.flickr.com//photos/igemhq/sets/72157625225590751/show/

–
–
–
–

High school
Undergraduate
Overgraduate
DIY / Community Lab
section planned for
2014
Ask a Biosafety Expert

Outreach, education, and good, practical biosafety advice
http://ask.diybio.org
Some of the questions so far…
How can I tell if the bacteria that
I’m growing on agar plates in my
room are toxic?
How should I dispose of my
bacterial plates if I don’t have
access to an autoclave?
How can I make bioart pieces with
embedded non-pathogenic
bacteria safe to sell to the public?
Real Opportunities for
Biosafety Professionals
• Movement is growing rapidly, but is still
relatively small
• An opportunity to build in safety culture
from the earliest stages
• Challenging questions, new technology,
new contexts, new types of clients, open
access resources
Call for Volunteers
please join our expert advisory board
•Required training and experience
–
–
–
–

All levels
RBP and CBSP highly desirable
Different roles for different levels of training,
experience, certification
Need diverse mix of domain expertise

•Minimum volunteer commitments
–
–
–

respond to 1 question per month
Monthly 1 hour conference call
~5 hours per month

•About the Organization
–
–
–

DIYbio.org is nonprofit corporation
ABE is a free educational service
Insured
BIG THANKS TO OUR CURRENT
ADVISORY BOARD MEMBERS
Molly Stitt-Fischer, Ph.D., CPH.
Ted Myatt, Sc.D., RBP.
J. Craig Reed, Ph.D., RBP.
Special thanks to Woodrow Wilson Center for
International Scholars, Synthetic Biology Project, Dave
Rejeski and Todd Kuiken.
Email: jason@diybio.org
Twitter: @jasonbobe
21

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Ask a Biosafety Expert Service at ABSA 2013

  • 1. October 23, 2013 Ask a Biosafety Expert: User-driven advisory service for the Do-It-Yourself Biology (DIYbio) Community ABSA Annual Meeting Kansas City, MO Jason Bobe Co-founder, DIYbio.org jason@diybio.org twitter:@jasonbobe 1
  • 2. New Teen Hobby: DIY DNA labs Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
  • 3. New Teen Hobby: DIY DNA labs Photo credit: Fred Turner, 2013 UK Young Engineer of the Year
  • 4. Dawn of the Biohackers. Discover Magazine. Oct 5, 2011. Photo by Grant Delin
  • 5. Vibrant Hobby Communities CHEMISTRY Lab Technician Set for Girls c. 1958 PERSONAL COMPUTING Homebrew Computer Club September 1976 ASTRONOMY Hanny’s Voorwerp discovered August 13, 2007 ROCKETRY Amateur Saturn V launch in MD April 25, 2009 Credits: Chemical Heritage Foundation; DigiBarn Computer Museum; Sloan Digital Sky Survey; Dick Stafford, RocketryPlanet.com.
  • 6. Biology is Next: Global DIYbio Community NORTH AMERICA Atlanta Baltimore Boston Brooklyn Cambridge Carlsbad Chicago Houston Los Angeles Nashville New York City Oakland Portland San Diego San Francisco Seattle Sunnyvale Toronto Vancouver Victoria EUROPE Budapest Copenhagen Cork Eindhoven Graz Groningen Kiev Lausanne London Manchester Munich Namur The Hague Paris Prague Switzerland ASIA Singapore Tel-Aviv OCEANIA Auckland Sydney
  • 7. Scope of Activities Exploratory Biology Environmental Sensing Personal BioMonitoring Constructive Biology Re-imagining Laboratory Devices Genetic Engineering 7
  • 8. Home Workshops 1.Garage: cancer biotech start-up 2.Closet: Personal genotyping facility 3.Kitchen: yogurt engineered to sense melamine (toxin) 4.Dorm: R&D lab for low-cost PCR machine
  • 9. Community Labs Genspace (Brooklyn, NY) NYTimes. December 19, 2010. Photo Michael Nagle
  • 10. Community Labs BioCurious (Sunnyvale, CA) http://biocuriosity.files.wordpress.com/2011/07/welcome-to-biocurious-sign.jpg?w=630 http://biocuriosity.files.wordpress.com/2011/09/img_0551.jpg?w=630
  • 11. DIY BioPrinter Biocurious Project: BioPrinter 6/ 2/ 13 2:50 PM (/files/deriv/FK7/UUYW/HAQ3D3X1/FK7UUYWHAQ3D3X1.LARGE.jpg) http://www.instructables.com/id/DIY-BioPrinter/ (/files/deriv/F8C/V1Y8/HBXWRTDL/F8CV1Y8HBXWRTDL.LARGE.jpg) (/files/deriv/F7P/LTPM/HAQ3D3Z2/F7PLTPMHAQ3D3Z2.LARGE.jpg) http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p http://www.technologyreview.com/sites/default/files/styles/view_body_embed/p ublic/images/bioprinter_0.jpg?itok=ZkO-8Tay ublic/images/biocurious.jpg?itok=CAGwqHG9
  • 12. Innovation Engine: Small Start-Ups Creating Low-Cost Kits & Devices --> Crowdsourcing $$$ Open GelBox 2.0 Microbial Fuel Cell Dremelfuge OpenPCR LavaAmp
  • 13. iGEM: International Genetically Engineered Machines Competition • 3200 active members • 215 teams & 200 labs • Sections http://2012.igem.org/wiki/images/6/65/Biobrick_trophy.jpg http://www.flickr.com//photos/igemhq/sets/72157625225590751/show/ – – – – High school Undergraduate Overgraduate DIY / Community Lab section planned for 2014
  • 14. Ask a Biosafety Expert Outreach, education, and good, practical biosafety advice http://ask.diybio.org
  • 15. Some of the questions so far…
  • 16. How can I tell if the bacteria that I’m growing on agar plates in my room are toxic?
  • 17. How should I dispose of my bacterial plates if I don’t have access to an autoclave?
  • 18. How can I make bioart pieces with embedded non-pathogenic bacteria safe to sell to the public?
  • 19. Real Opportunities for Biosafety Professionals • Movement is growing rapidly, but is still relatively small • An opportunity to build in safety culture from the earliest stages • Challenging questions, new technology, new contexts, new types of clients, open access resources
  • 20. Call for Volunteers please join our expert advisory board •Required training and experience – – – – All levels RBP and CBSP highly desirable Different roles for different levels of training, experience, certification Need diverse mix of domain expertise •Minimum volunteer commitments – – – respond to 1 question per month Monthly 1 hour conference call ~5 hours per month •About the Organization – – – DIYbio.org is nonprofit corporation ABE is a free educational service Insured
  • 21. BIG THANKS TO OUR CURRENT ADVISORY BOARD MEMBERS Molly Stitt-Fischer, Ph.D., CPH. Ted Myatt, Sc.D., RBP. J. Craig Reed, Ph.D., RBP. Special thanks to Woodrow Wilson Center for International Scholars, Synthetic Biology Project, Dave Rejeski and Todd Kuiken. Email: jason@diybio.org Twitter: @jasonbobe 21

Notes de l'éditeur

  1. {"12":"From Left to Right, Top to Bottom\nPearl Biotech, GelBox\nMFC\nOpenPCR\nDremelfuge\n(5) LavaAmp\n","8":"(1) A cancer biotech company incubated its business in a garage in Mountainview, although they have since vacated it and moved to a commercial facility. (2) Kay Aull built a small lab in her bedroom closet in Cambridge, MA and used it to perform allele-specific genotyping for HFE, a disease that runs in her family. (3) Meredith Patterson working from her kitchen in the Bay Area on a project to engineer yogurt bacteria to sense the food contaminant, melamine. (4) The start-up company OTYP, an educational biotechnology business, ran an R&D lab out of the founder’s college apartment in Ann Arbor.\nhttp://www.flickr.com/photos/sugargliders/4561317461/in/pool-1296598@N22/\nhttp://www.boston.com/news/science/articles/2009/05/11/do_it_yourself_genetic_sleuthing/\nhttp://www.msnbc.msn.com/id/28390773/ns/technology_and_science-innovation/\nhttp://www.biotechniques.com/news/biotechniquesNews/biotechniques-301745.html\n","3":"Issues that a Biosafety Professional Sees Here:\n1. Unlabeled, unknown solutions/chemicals\n2. Potential electrical hazard\nIs this thermometer filled with mercury?\nUsing a household air purifier – to attempt to control what kind of hazard? Chemical fumes = won’t work b/c HEPA filters won’t capture vapors only particles\nOutdated (unsafe?) centrifuge\nHeat block if left on at high temperature = fire hazard, especially if lab is in garage with paint/solvents/gasoline stored nearby.\n","10":"San Francisco community lab – membership to use lab and equipment\nBuy your own reagents\nSafety training and intro courses\n"}