1. Table of contents
MACS® Products for tissue regeneration research 3
MACS Technology—the complete solution 4
MACS Cell Separation Strategies 5
Human stem cells—sample preparation, cell separation and analysis 6
Embryonic stem cells and induced pluripotent stem cells—separation and analysis 7
Mesenchymal stromal cells—separation and analysis 8
Endothelial progenitor cells—separation and analysis 9
Tissue regeneration—examples 10
References 12
MACS Products for sample preparation, cell separation, and cell analysis 13
MACS Products for cell culture 14
Molecular biology products and services 15
Cover image: SPL/Agentur Focus
2. MACS® Technology
MACS® Products for tissue regeneration research
MACS® Products provide the basis for reliable
methods to investigate the full potential of stem MACS Products provide the tools that open up new
cells in tissue regeneration research. perspectives for tissue regeneration research:
Stem and progenitor cells have the potential to revolutionize • Reliable isolation of defined stem cell populations
tissue regeneration and engineering in the future. The with excellent purity for reproducible results
understanding of stem cell biology is of paramount importance • Stem cells and progenitors thereof can be isolated
for the development of stem cell–based therapies. The within an hour rather than several days,
multipotent—and even pluripotent—differentiation capacity saving valuable time for you to focus on your research.
of certain stem and progenitor cell populations makes them a
potential key to successful regeneration of many tissue types. • Isolated cells can immediately be used for cell culture
Over the coming decade, research into this field may take center and animal experiments; cells remain viable and fully
stage as the new frontier in the treatment of many disabling functional.
diseases and injuries. • Miltenyi Biotec offers integrated solutions for a wide
Reproducible data, standardized protocols, and defined variety of research needs—from sample preparation
cell sources are key to unravel the full potential of stem and to molecular analysis.
progenitor cells. However, most stem cell sources consist of
heterogenous cell populations with varying differentiation
and regeneration potential. The availability of defined cell
populations with specific differentiation capacities will
therefore be crucial for the targeted regeneration of tissues.
In turn, this will facilitate a controlled and optimized growth
and differentiation of stem cells into their target tissue types,
especially during ex vivo tissue development. The separation of
defined pre-committed stem and progenitor cell populations
derived from stem cell populations with pluripotent or
multipotent differentiation potential would be beneficial for
future tissue regeneration applications.
Miltenyi Biotec offers numerous innovative products for the
isolation of stem and progenitor cells as well as for sample
preparation, cell analysis, cell culture, and molecular analysis.
3
3. MACS® Technology
MACS® Technology —the complete solution
Since its introduction in 1989, MACS® Technology has become the gold standard for cell separation.
Nowadays, Miltenyi Biotec stands for more than cell separation, offering more than 1000 innovative
research products for biomedical research and life sciences. The MACS Research Product portfolio
includes instruments and reagents for sample preparation, cell separation, cell analysis, cell culture,
and molecular biology. Miltenyi Biotec also provides tools for clinical-scale cell separation based on
MACS Technology. Miltenyi Biotec has a strong commitment to continual product development with
regards to current and future basic and clinical research.
MACS Sample Preparation
The quality of an experiment strictly depends on the
quality of the sample preparation.
Miltenyi Biotec offers innovative instruments and
reagents for fast and gentle preparation of samples from
solid tissues as well as cultured cells.
MACS Cell Separation
A large panel of MACS MicroBeads and MicroBead Kits is
available for the isolation of virtually any cell type.
The cells can be separated manually or in an automated
fashion. The new autoMACS™ Pro Separator has been
designed for automated walk-away cell sorting of
multiple samples.
MACS Cell Analysis
Miltenyi Biotec provides a large panel of monoclonal
antibodies and kits for fluorescence microscopy and flow
cytometry. The innovative MACSQuant™ Analyzer is an
extremely compact, easy-to-use, multicolor benchtop
cell analyzer. The instrument is fully automated and
enables absolute cell count.
MACS Cell Culture
The product portfolio for cell culture includes media as
well as recombinant cytokines and growth factors.
MACSmolecular
Miltenyi Biotec provides products for analytical protein
isolation and detection, mRNA purification and
amplification, cDNA synthesis and labeling, microRNA
analysis, as well as microarray technologies and
instrumentation. The portfolio includes genomics
services, such as gene expression and microRNA analysis
microarrays, array-CGH, and bioinformatics.
4
4. MACS® Cell Separation Strategies
MACS® Technology—the gold standard
Benefits of MACS® Technology at a glance: in cell separation
• Fast—cell separation takes less than one hour MACS Technology is based on MACS MicroBeads, MACS
• Gentle—separated cells remain viable and functional Columns, and MACS Separators—strong permanent magnets.
• Flexible—both labeled and unlabeled fractions can be MicroBeads are superparamagnetic particles coupled to specific
obtained with excellent purity and high recovery monoclonal antibodies.
• Easy separation of large cell numbers—up to 109 Target cells can be magnetically isolated by positive selection
labeled cells per column using specific cell surface antigens or by depletion of unwanted
cells in order to obtain untouched cells. Furthermore, these two
separation strategies can easily be combined to provide greater
MACS Technology
flexibility for the sequential sorting of complex subpopulations
Magnetic labeling of cells.
Cells of interest are labeled with
MACS® MicroBeads in a short MACS MicroBeads
incubation step.
• Highly specific monoclonal antibody conjugates
• Small (50 nm), virus-sized nanoparticles
• Non-toxic, biodegradable
Magnetic separation
Labeled and unlabeled cells are
• Colloidal, for ease of handling and short incubation times
separated on a MACS Column
placed in the magnetic field of a MACS Columns and MACS Separators
MACS Separator.
• Optimal recovery and high purity with MACS Columns
The flow-through can be collected • Gentle to cells
as the non-magnetic, unlabeled
cell fraction. • Automated cell separation with
autoMACS™ Pro Separator
Elution of the labeled cell fraction
The separation column is removed
from the magnetic field and the
retained cells are flushed out.
Both the labeled and unlabeled
fractions can be recovered and
used for downstream applications.
MidiMACS™ Separator autoMACS™ Pro Separator
MACS MicroBeads for indirect magnetic labeling
For maximum flexibility, indirect magnetic labeling with MACS
MicroBeads allows the use of any primary antibody. Monoclonal
or polyclonal primary antibodies can be either unconjugated,
biotinylated, or fluorochrome-conjugated.
MACS MicroBeads are nano-sized
particles and are barely detectable
by scanning electron microscopy.
The micrograph shows a lymphocyte
isolated by positive selection (left).
Transmission electron micrograph
of an isolated lymphocyte with
MicroBeads (arrow) on the cell surface
(right).
(Courtesy of Prof. Groscurth, Zürich, CH.)
5
5. Sample preparation,
cell separation, cell analysis
Human stem cells—sample preparation, cell separation and analysis
Human stem cells in tissue regeneration research
Stem and progenitor cells have the potential to revolutionize tissue regeneration
and engineering in the future. To support research into the biology of stem
cells and to explore their extraordinary capacity for future tissue regeneration
applications, it is crucial to develop reliable methods for the specific isolation
of distinct stem and progenitor cell populations with defined differentiation
potential. Likewise, the isolation of their differentiated progenies is of immense
interest for research into future clinical applications.
For stem cell research, Miltenyi Biotec has developed numerous innovative
tools that allow straightforward sample preparation, cell separation, as well as
subsequent cell analysis.
Sample preparation
For a broad range of tissues that are of interest for current tissue regeneration research,
the ideal cell sources have yet to be determined. Therefore, the isolation of stem cells from
embryonic, fetal, and adult sources is of great importance.
Preparing suitable suspensions of viable single cells from tissues is a critical step for
the successful isolation of tissue-specific stem cells. Miltenyi Biotec offers the new
gentleMACS™ Dissociator for the gentle and efficient dissociation of tissues. In addition, the
Neural Tissue Dissociation Kits, Pre-Separation Filters, and the Dead Cell Removal Kit allow
an optimal preparation of single-cell suspensions for subsequent separation.
gentleMACS™ Dissociator
The gentleMACS™ Dissociator is a benchtop instrument for the automated dissociation
of tissues. Two types of unique gentleMACS Tubes are available for the instrument and
enable the time-saving and easy dissociation of tissues into single-cell suspensions or
homogenization of tissues to lysates in a closed system.
The range of protocols available for the gentle and efficient dissociation of tissues is
continuously expanding. Protocols are optimized to yield single-cell suspensions with high
viability rate. The gentleMACS Dissociator can further be used for the homogenization
of tissues or cells to extract biomolecules for molecular biology experiments, such as the
isolation of total RNA or mRNA.
gentleMACS Dissociator—features at a glance
• Time-saving automated tissue dissociation or homogenization
The gentleMACS Dissociator—the gentle way of
automated tissue dissociation • Standardized procedure
• Reliable and reproducible results
• High level of user safety
• Sterile sample handling
Cell separation and analysis
For research into future tissue regeneration applications, it is crucial to develop reliable
methods for the specific isolation of distinct stem and progenitor cell populations with
defined differentiation potential as well as their differentiated progenies.
Miltenyi Biotec offers numerous tools for the isolation of stem and progenitor cells
according to specific cell surface markers. For maximum flexibility, indirect magnetic
labeling with MACS MicroBeads allows the use of any primary antibody. Monoclonal or
polyclonal primary antibodies of choice can be either unconjugated, biotinylated, or
fluorochrome-conjugated. Furthermore, a large variety of products is available for the
analysis of stem and progenitor cells.
6
6. Cell separation and analysis
Embryonic stem cells and induced pluripotent stem cells—
separation and analysis
Embryonic stem cells and induced pluripotent stem cells
Pluripotent embryonic stem cells (ESCs), derived from the inner cell mass of the blastocyst,
and induced pluripotent stem (iPS) cells are known for their capacity to differentiate into
virtually all cell types of the body and to self-renew while maintaining a stable karyotype.
Therefore, these cells are of special interest for basic tissue regeneration research to
understand developmental processes, and might provide the basis for clinical applications
Cell separation in the future. For these applications it is crucial to use homogenous cell populations. MACS®
Cell analysis Technology enables the isolation of numerous particular cell types and subsets at high
microRNA expression profiling
purity—ready for in vitro and animal experiments.
Gene expression analysis
Pluripotent cells express the surface markers SSEA-3, SSEA-4, Tra-1-60, Tra-1-81, but not
CD15 (SSEA-1).1–3 The positive selection, for example, according to the expression of SSEA-4
using MACS MicroBeads for indirect magnetic labeling is a proven strategy for the isolation
of pluripotent cells.4,5 These markers can also be used for the depletion of pluripotent
cells from differentiated cultures, thereby reducing the risk of teratoma formation in
transplantation experiments.
For more information on Miltenyi Biotec products
for ESC and iPS cell research, please refer to the After their in vitro differentiation, ESC- or iPS-derived cells exhibit a broad cellular
corresponding brochure that is available for
download at www.miltenyibiotec.com. heterogeneity with respect to developmental stages and lineage specification. This leads
to highly heterogenous cell populations. Cell separation according to the expression of
certain surface markers can enrich target cells or deplete unwanted populations:
Hematopoietic stem cells (HSCs) can be isolated from differentiated ESCs by positive
selection according to the expression of, for example, CD34 or CD133.6–12
Endothelial progenitor cells (EPCs) can be separated from ESC cultures by using, for
example, the cell surface markers CD347,8,13 and CD31.
Early neural cell populations were enriched with FORSE-1 antibodies and MicroBeads
for indirect magnetic labeling. Various markers that indicate the developmental maturity
after neuronal differentiation have been described:
CD133+, CD271+, or CD146+ cells showed characteristics of neural stem or precursor cells,
A2B5+ cells showed characteristics of glial-restricted precursor cells, whereas CD56+, PSA-
NCAM+, or CD24+ cells showed characteristics of differentiated neurons.14
Cardiomyocytes might be separable from heterogenous ESC cultures according to
CD56+ expression.15
7
7. Cell separation and analysis
Mesenchymal stromal cells—separation and analysis
Mesenchymal stromal cells
Mesenchymal stromal cells (MSCs) can be obtained from a variety of tissue sources
including bone marrow aspirate16,17, umbilical cord blood17, and even lipoaspirate18,19.
MSCs have shown the potential to differentiate into a variety of nonhematopoietic tissue
types, including bone or cartilage. Numerous cell surface antigens have been used for the
isolation of MSCs from various sources.
Sample preparation
Cell separation Bone marrow
Cell analysis CD271 is a well-known marker for the isolation of MSCs from bone marrow. 20–22
Cell culture
Marrow stromal antigen-1 (MSCA-1), which is recognized by clone W8B2, was shown to be
Expression profiling
restricted to MSCs in the CD271bright population in bone marrow.22 These CD271brightCD45dim
MSCs show a higher clonogenic capacity compared to the CD271+CD45+ fraction.22
Therefore, MSCA-1 is a suitable marker for the identification of MSCs with a high
proliferative potential.
Additional markers for the isolation of MSCs from bone marrow include CD117 23, CD105 24,25,
For more information on Miltenyi Biotec Stro-1 26 , CD146 27, and CD13328.
products for MSC research, please refer to the
corresponding brochure available for download
at www.miltenyibiotec.com. Multipotent adult progenitor cells (MAPCs) can be enriched from bone marrow by
depletion of CD235a (glycophorin A) and CD45. 33
Cord blood
BM-MNCs before separation
CD133 is a suitable marker for the identification of MSCs from cord blood.17
Lipoaspirate
Various strategies can be pursued for the isolation of MSCs from lipoaspirate: positive
CD45-FITC
selection of CD271+ cells 29 or CD146+ cells, positive selection of CD34+ cells30, as well as
depletion of CD45+ and CD31+ cells. 18,31
Dental pulp
STRO-1 and CD146 are appropriate markers for the separation of MSCs from dental pulp. 27
Anti-MSCA-1 (W8B2)-APC
MSCA-1+ cells Amniocentesis cultures
MSC-like fetal-derived stem cells from amniocentesis cultures can be isolated according to
the expression of CD117 and share many markers of adult and embryonic stem cells. Fetal-
derived stem cells retain a multipotent ability to differentiate into cell types that represent
CD45-FITC
all embryonic germ layers. 32
Anti-MSCA-1 (W8B2)-APC
MSCA-1 (W8B2)+ cells were isolated from human
bone marrow mononuclear cells (BM-MNCs)
using the MSC Research Tool Box – MSCA-1
(W8B2). Cells were stained with Anti-MSCA-1
(W8B2)-APC and CD45-FITC.
8
8. Endothelial progenitor cells—separation and analysis
Endothelial progenitor cells
Regeneration of vascular tissue is an important topic in
therapeutic research, especially for the potential treatment of
peripheral vascular disease and the revascularization of ischemic
tissues, for example, in the heart.
Endothelial progenitor cells (EPCs) have been suggested to
play an important role in postnatal neoangiogenesis and
neovascularization. Therefore, EPCs have come into focus for
the potential treatment of ischemic or injured tissue and for the
coating of scaffolds to increase biocompatibility of biomaterials.
EPCs are defined by the expression of the markers CD34 and
CD309 (VEGFR-2/KDR). Analysis of CD133 expression allows the
distinction between early and matured EPCs in human. 34,35 EPCs
were enriched according to their expression of CD34 or CD133
from different hematopoietic sources34–38,43. CD133+ cells have
been used in studies that show a significantly improved vascular
SPECT (single-photon emission computed tomographic) scan from the posterior
wall area of a human heart. Courtesy of Prof. Gustav Steinhoff, Rostock, Germany. network restoration in an ischemic hind limb rat model38 and an
ischemic heart mouse model39. Furthermore, CD133+ cells were
used in combination with biodegradable scaffolds for the three-
dimensional tissue engineering of microvessels.40 Safety and
feasibility and, moreover, efficacy was shown in several clinical
trials investigating CD133+ cell therapy combined with coronary
artery bypass grafting41,42 and cell therapy alone43.
9
9. Tissue regeneration research
Tissue regeneration research—examples
Tissue-resident stem cells have been found in almost any tissue.
A
A better understanding of their biology and the development
of methods for their isolation and expansion might therefore be
useful for tissue regeneration. In addition, stem cells from the
hematopoietic system have been described to contribute to the
regeneration of tissues. Following, a few examples for research
on the regeneration of certain tissues are presented.
Liver tissue
In contrast to other organs, the liver is known for its capacity
to regenerate in situ. This is due to the population of stem cells
within the liver. These stem cells have been successfully isolated
using MACS® Technology by targeting CD326 (EpCAM)44,45,
CD13345, or CD117.46 The transplantation of bone marrow–derived
B
CD133 + cells has been shown to benefit the expansion of liver
tissue in situ prior to partial hepatectomy.47
Muscle tissue
The identification and isolation of muscle stem and progenitor
cells by the markers CD13348 and CD5649,50, respectively, is of
importance for research on smooth, skeletal, and even cardiac
muscle tissue regeneration. After transplantation into mouse
models, human CD133 + stem cells from peripheral blood and
circulating endothelial progenitor cells (cEPCs) from umbilical
cord blood were capable of regenerating in situ dystrophic
CD133+ cells, isolated from mobilized peripheral blood, gave rise to adherent cells muscle tissue51 and skeletal muscle52, respectively. Cardiac
after 3–5 weeks of cultivation. muscle regeneration is a prime goal in cardiovascular disease
These cells were able to differentiate into hepatocyte-like cells.
(A) The cells are stained for hepatocyte nuclear factor-3 (FITC), albumin (Cy3), and research; functional cardiomyocytes can be generated in vitro
nuclei (DAPI).
(B) The cells are stained for cytokeratin 19 (Cy3) and nuclei (DAPI) (200×).
from ESCs15, CD133 + fetal liver cells53, and from CD34 + cEPCs from
(Courtesy of Selim Kuçi, Tübingen, Germany.) peripheral blood54.
CD133+ cells isolated from mobilized PBMCs were cultivated for 3–5 weeks.
Adherent cells were able to differentiate into skeletal muscle–like cells. The cells
are stained for desmin (FITC), actin (Cy3), and nuclei (DAPI)(200×).
(Courtesy of Selim Kuçi, Tübingen, Germany.)
10
10. Tissue regeneration research—examples
Neural tissue
A
The regeneration of neural tissue has far-reaching consequences
for the potential treatment of debilitating neurodegenerative
diseases or injuries, including stroke and spinal cord damage.
Human neural stem cells that were isolated from fetal brain
according to CD133 expression have been shown to differentiate
in vitro and in vivo into cells with neural phenotypes and even
restore the function of damaged spinal tissue in mice. 55
Furthermore, CD133 + cells isolated from mobilized peripheral
blood56 or skin57 can be differentiated into neural lineages.
Neuronal-committed precursors from mammalian brain can be
magnetically isolated for research purposes via the depletion
of A2B5 + cells followed by the positive selection of PSA-NCAM+
cells.58
B C
CD133+ cells isolated from mobilized PBMCs were cultivated for 3–5 weeks.
Adherent cells were able to differentiate into neural-like cells.
(A) Astrocyte-like cells stained for GFAP (Cy3), EPO (FITC), and nuclei (DAPI).
(B) Oligodendrocyte-like cells stained with GFAP (FITC), MBP (Cy3), and DAPI
(nuclei).
(C) Neuronal-like cells stained for betatubulin III (Cy3) and nuclei (DAPI)(200×).
(Courtesy of Selim Kuçi, Tübingen, Germany.)
For more information on Miltenyi Biotec
products for neuroscience research, please refer
to the corresponding brochure that is available
for download at www.miltenyibiotec.com.
11
11. References
MACS® Technology
References
1. Kaufman, D. S. et al. (2001) Proc. Natl. Acad. Sci. USA 98: 10716–10721.
2. Chang, K. et al. (2006) Blood 108: 1515–1523.
3. Carpenter, M. K. et al. (2003) Cloning Stem Cells 5: 79–88.
4. Cheng, L. et al. (2003) Stem Cells 21: 131–42.
5. Schulz, T. C. et al. (2004) Stem Cells 22: 1218–1238.
6. Bandi, S. and Akkina, R. (2008) AIDS Res. Ther. 5: 1.
7. Wang, Z. Z. et al. (2007) Nat. Biotechnol. 25: 317–318.
8. Chen, T. et al. (2007) Stem Cells 25: 392–401.
9. Narayan, D. A. et al. (2006) Blood 107: 2180–2183.
10. Galic, Z. et al. (2006) Proc. Natl. Acad. Sci. USA 103: 11742–11747.
11. Kaufman, D. S. et al. (2001) Proc. Natl. Acad. Sci. USA 98: 10716–10717.
12. Vodyanik, M. A. et al. (2006) Blood 108: 2095–2105.
13. Ferreira, L. S. et al. (2007) Circ. Res. 101: 286–294.
14. Pruszak, J. et al. (2007) Stem Cells 25: 2257–2268.
15. Xu, S. et al. (2006) Stem Cells and Dev. 15: 631–639.
16. Jones, E. A et al. (2002) Arthritis Rheum. 46: 3349–3360.
17. Tondreau, T. et al. (2005) Stem Cells 23: 1105–1112.
18. Boquest, A. C. et al. (2005) Mol. Biol. Cell 16: 1131–1141.
19. Meyerrose, T. E. et al. (2007) Stem Cells 25: 220–227.
20. Jones, E. A. et al. (2006) Cytometry B Clin. Cytom. 70: 391–399.
21. Quirici, N. et al. (2002) Exp. Hematol. 30: 783–791.
22. Bühring, H. J. et al. (2007) Ann. NY Acad. Sci. 1106: 262–271.
23. Huss, R. and Moosmann, S. (2002) Br. J. Hematol. 118: 305–312.
24. Aslan, H. et al. (2006) Stem Cells 24: 1728–1737.
25. Majumdar, M. K. et al. (2003) J. Biomed. Sci. 10: 228–24.
26. Gronthos, S. et al. (1994) Blood 84: 4164–4173.
27. Shi, S. and Gronthos, S. (2003) J. Bone Miner. Res. 18: 696–704.
28. Pozzobon, M. et al. (2008) Stem Cells and Dev. (in press).
29. Godthardt, K. (2007) MSC 2007 Adult Mesenchymal Stem Cells
in Regenerative Medicine, Cleveland, Poster Nr. 301, Session III.
30. Astori, G. et al. (2007) J. Transl. Med. 5: 55.
31. Noer, A. et al. (2006) Mol. Biol. Cell 17: 3543–3556.
32. De Coppi, P. et al. (2007) Nat. Biotechnol. 25: 100–106.
33. Reyes, M. et al. (2002) J. Clin. Invest. 109 : 337–346.
34. Peichev, M. et al. (2000) Blood 95: 952–958.
35. Rafii, S. and Lyden, D. (2003) Nat. Med. 9: 702–712.
36. Gehling, U. et al. (2000) Blood 95: 3106–3112.
37. Taguchi, A. et al. (2004) J. Clin. Invest. 330–338.
38. Suuronen, E. et al. (2006) Circulation. 114 (suppl. 1): 138–144.
39. Ma, N. et al. (2006) Cardiovasc. Res. 71: 158–169.
40. Wu, X. et al. (2004) Am. J. Physiol. Heart. Circ. Physiol. 287: H480–H487.
41. Stamm, C. et al. (2004) Thorac. Cardiovasc. Surg. 52: 152–158.
42. Stamm, C. et al. (2007) J. Thorac. Cardiovasc. Surg. 133: 717–725.
43. Klein, H. M. et al. (2007) Heart Surg. Forum 10: E66–69.
44. Schmelzer, E. et al.(2007) J. Exp. Med. 204: 1973–1987.
45. Schmelzer, E. et al. (2006) Stem Cells 24: 1852–1858.
46. Laurson, J. et al. (2005) Int. J. Exp. Pathol. 86: 1–18.
47. Schulte am Esch, J. et al. (2005) Stem Cells 23: 463–470.
48. Alessandri, G. et al. (2004) Lancet 364: 1872–1883.
49. Sinanan, A. et al. (2004) Biotechnol. Appl. Biochem. 40: 25–34.
50. De Luna, N. et al. (2006) J. Biol. Chem. 281: 17092–17098.
51. Torrente, Y. et al. (2004) J. Clin. Invest. 114: 182–195.
52. Pesce, M. et al. (2003) Circ. Res. 93: 51–62.
53. Schmelkov, S. V. et al. (2005) Circulation 111: 1175–1183.
54. Badorff, C. et al. (2003) Circulation 107: 1024–1032.
55. Cummings, B. J. et al. (2005) Proc. Natl. Acad. Sci. USA 102: 14069–14074.
56. Kuçi, S. et al. (2003) MACS&more 7/1: 6–8.
57. Belicchi, M. et al. (2004) J. Neurosci. Res. 77: 475–486.
58. Seidenfaden, R. et al. (2006) Mol. Cell Neurosci. 32: 187–198.
12
12. MACS® Products
MACS® Products for sample preparation, cell separation, and cell analysis
Product Components or capacity Order no. Product Order no.
gentleMACS™ gentleMACS™ Dissociator 130-093-235 Anti-FITC MicroBeads 130-048-701
Starting Kit C Tubes, 25 pieces Anti-PE MicroBeads 130-048-801
M Tubes , 25 pieces
Power cord Anti-APC MicroBeads 130-090-855
User manual
gentleMACS Protocols Anti-Cy5/Anti-Alexa Fluor 647 MicroBeads 130-091-395
C Tubes C Tubes, 25 pieces, single- 130-093-237 Anti-Cy7 MicroBeads 130-091-652
packed Anti-Biotin MicroBeads 130-090-485
M Tubes M Tubes, 25 pieces, single- 130-093-236 Streptavidin MicroBeads 130-048-101
packed 130-093-458
M Tubes, 50 pieces per bag Rat Anti-Mouse IgG1 MicroBeads 130-047-101
Neural Tissue 50 tests with up to 400 mg of 130-092-628 Rat Anti-Mouse IgG2a+b MicroBeads 130-047-201
Dissociation Kit (P) neural tissue
Rat Anti-Mouse IgM MicroBeads 130-047-301
Neural Tissue 50 tests with up to 400 mg of 130-093-231
Goat Anti-Mouse IgG MicroBeads 130-048-401
Dissociation Kit (T) neural tissue
Mouse Anti-Rat Kappa MicroBeads 130-047-401
Pre-Separation 50 filters 130-041-407
Filters Goat Anti-Rat IgG MicroBeads 130-048-501
Dead Cell For 10⁹ total cells 130-090-101 Goat Anti-Rabbit IgG MicroBeads 130-048-602
Removal Kit
MACS Products for indirect magnetic labeling
MACS Products for sample preparation
Product Order no. Product Order no.
Anti-A2B5 MicroBeads, human, mouse, rat 130-093-388 Anti-A2B5-PE, -APC, pure, human, 130-093-581, 130-093-582,
mouse, rat 130-092-394
MSC Research Tool Box – MSCA-1 (W8B2), 130-093-572
human Anti-MSCA-1 (W8B2)-FITC, -PE, -APC, 130-093-585, 130-093-587,
-Biotin, pure, human 130-093-589, 130-093-593,
Anti-MSCA-1 (W8B2) MicroBead Kit, human 130-093-583 130-093-595
Anti-PSA-NCAM MicroBeads, 130-092-966 Anti-PSA-NCAM-PE, -APC, human, 130-093-274, 130-093-273
human, mouse, rat mouse, rat
CD31 MicroBead Kit, human 130-091-935 CD31-FITC, -PE, -APC, human 130-092-64, 130-092-653,
CD34 MicroBead Kit, human 130-046-702 130-092-652
CD34 MultiSort Kit, human 130-056-701 CD34-FITC, -PE, -APC, human 130-081-001, 130-081-002,
130-090-954
CD45 MicroBeads, human 130-045-801
CD56-PE, APC, pure, human 130-090-755, 130-090-843,
CD56 MicroBeads, human 130-050-401 130-090-955
CD105 MicroBeads, human 130-051-201 CD117 (A3C6E2)-PE, -APC, human 130-091-734, 130-091-733
CD117 MicroBead Kit, human 130-091-332 CD117 (AC126)-PE, human 130-091-735
CD133 MicroBead Kit, human 130-050-801 CD133/1 (AC133)-PE, -APC, -Biotin, 130-080-801, 130-090-826,
pure, human 130-090-664, 130-090-422
CD146 MicroBead Kit, human 130-093-596
CD133/2 (293C3)-PE, -APC, -Biotin, 130-090-853, 130-090-854,
CD235a (Glycophorin A) MicroBeads, human 130-050-501
pure, human 130-090-852, 130-090-851
MSC Research Tool Box – CD271 (PE), human 130-092-867
CD133/1 (W6B3C1) pure, human 130-092-395
MSC Research Tool Box – CD271 (APC), human 130-092-291
CD146-FITC, -PE, -APC, -Biotin, pure, 130-092-851, 130-092-853,
CD271 MicroBead Kit (PE), human 130-092-819 human 130-092-849, 130-092-852,
130-092-850
Lineage Cell Depletion Kit, human 130-092-211
CD271 (LNGFR), -PE, -APC, -Biotin, 130-091-885, 130-091-884,
CD271 MicroBead Kit (APC), human 130-092-283 human 130-091-883
CD326 (EpCAM) MicroBeads, human 130-061-101 CD309 (VEGFR/KDR)-PE, -APC, -Biotin, Coming soon
human
MACS Products for the isolation of stem and progenitor cells
CD326 (EpCAM)-FITC, -PE, -APC, 130-080-301, 130-091-253,
human 130-091-254
EPC Enrichment and Enumeration Kit, 130-093-477
human
MACS Products for the analysis of stem and progenitor cells
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13. Products
MACS® Technology
MACS® Products for cell culture
NH media for the enumeration, expansion, and
MACS® NH Media enable the differentiation of mesenchymal stromal
cells (MSCs) into functional nonhematopoietic (NH) cell types.
differentiation of MSCs
MSCs are of great importance to researchers working towards the
development of novel tissue regeneration therapies. However, in
order to properly evaluate the potential of MSCs completely, it is
crucial to establish standardized and reproducible procedures for
stem cell isolation and cultivation. MACS® NH Stem Cell Media are
optimized for the most convenient enumeration and expansion
of nonhematopoietic (NH) stem cells from a variety of sources,
including bone marrow aspirate, lipoaspirate, or potentially any
NH stem cell source, MSC enumeration
e.g., bone marrow, NH CFU-F Medium tissue or organ within the human body. Miltenyi Biotec also
lipoaspirate offers media to evaluate the differentiation potential of NH stem
cells during in vitro cultivation.
Media are available for the reliable and reproducible
differentiation of NH stem cells to adipocyte, chondrocyte, or
osteoblast lineages. These media can also be used to define the
full differentation capacity of an NH stem cell population: MSCs
must be able to form all three cell lineages.
MSC expansion
NH Expansion Medium MACS NH Media Order no.
NH CFU-F Medium 24×5 mL 130-091-676
NH Expansion Medium 500 mL 130-091-680
NH AdipoDiff Medium 100 mL 130-091-677
NH ChondroDiff Medium 100 mL 130-091-679
NH OsteoDiff Medium 100 mL 130-091-678
CytoMix – MSC, human
Adipocytes Chondrocytes Osteoblasts The CytoMix – MSC, human (130-093-552) is a composition of
NH AdipoDiff Medium NH ChondroDiff Medium NH OsteoDiff Medium cytokines for the most efficient and reproducible expansion of
human MSCs. In combination with the NH Expansion Medium,
CytoMix – MSC optimally supports the proliferation of human
MSCs, especially after separation, e.g., according to CD271 or
MSCA-1 (W8B2) expression using MACS® Technology.
MACS Basic Culture Media Order no.
Basic media and cytokines for stem cell culture
Miltenyi Biotec offers cell culture media for a broad spectrum
DMEM 500 mL 130-091-437
of applications. In addition, high-quality recombinant cytokines
DMEM with stable glutamine 500 mL 130-091-438 and growth factors are available, that are well-suited for various
RPMI 1640 500 mL 130-091-440 applications such as cell culture, differentiation studies, and
RPMI 1640 with stable glutamine 500 mL 130-091-439 biological assays. Selected products are available in a premium-
grade format with excellent purity and high, well-defined activity
MACS Cytokines and Growth Factors as well as in research-grade quality. For a complete list of
available cytokines, please visit
Human: BDNF; BMP-2; EGF; EG-VEGF; FGF-2; FGF-4; Flt3-Ligand;
G-CSF; GM-CSF; HGF; IL-3; IL-6; IL-11; PDGF-AA; PDGF-AB; PDGF-BB; SCF; http://www.miltenyibiotec.com/cytokines.
SDF-1α; TGF-α; TGF-β1; TGF-β3; TNF-α; VEGF (121 aa);
VEGF (165 aa)
Mouse: EGF; Flt3-Ligand, G-CSF; GM-CSF; IL-3 (135 aa); IL-6; SCF;
TNF-α; VEGF (164 aa); VEGF (165 aa);
Rat: VEGF-C; VEGF-C (C152S)
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14. Molecular biology products and services
Stem cell differentiation tracking by
gene expression profiling
MACSmolecular provides a highly innovative range of products
and services with a strong focus on gene expression profiling.
Particularly when isolating stem cells, sensitive downstream
analyses are required.
One-step mRNA isolation and in-column
cDNA synthesis
Premium mRNA is isolated within 15 minutes directly from
a-Hyb™ Hybridization Station cells or tissues. The µMACS™ One-step cDNA Kit combines
efficient magnetic isolation of mRNA with revolutionary in-
column cDNA synthesis. Purified cDNA can be generated
mRNA isolation/cDNA synthesis
from just a few to as many as 10⁷ cells.
µMACS mRNA Isolation Kit Small Scale 130-075-201
Large Scale 130-075-101
For Total RNA 130-075-102
PIQOR™ Stem Cell Microarray
The PIQOR™ Stem Cell Microarray comprises 942 relevant marker
µMACS mRNA Isolation Starting Kit 130-075-202
genes for human stem cells and their differentiation.
µMACS One-step cDNA Kit 130-091-902
It is available as a convenient microarray kit* or within the scope
µMACS One-step cDNA Starting Kit 130-091-989 of the microarray service**. Gene expression experiments allow
for the quality control of different stem cell types, comparison
PIQOR Microarray Kit * between different stages of differentiation, as well as the
PIQOR Stem Cell Microarray Kit, antisense optimization of differentiation protocols.
4 Microarrays 130-092-033
SuperAmp™ Service ***
8 Microarrays 130-092-034
When the number of stem cells for analysis is low, Miltenyi Biotec
PIQOR Microarray Service ** offers the ideal solution for gene expression profiling needs. The
SuperAmp™ Service (available as an extension of
Service Stem Cell Microarray Plus Amplification 160-000-765
the PIQOR™ Microarray Service) can reliably amplify mRNA
SuperAmp Amplification *** million-fold from as little as one cell. The service is ideal for stem
cells sorted with MACS® Technology, flow cytometry,
SuperAmp Service (per sample) 160-000-936
or even from laser capture microdissected tissue.
miRXplore Microarray Kit
miRXplore™ Kits and Services
4 Microarrays 130-093-254
Explore microRNA expression in human and mouse stem
8 Microarrays 130-093-272 cells with the new miRXplore™ Microarray Kits and Services.
Designed in collaboration with experts at the Rockefeller
miRXplore Microarray Services University¹, the microarray covers more than 2700 human,
miRXplore Microarray Service 160-001-143 mouse, rat, and viral microRNA sequences and possess rigorous
miRXplore Universal Reference Service 160-001-161 internal control system. Sequences differing by just one
oligonucleotide can be reproducibly detected and re-ratios
miRXplore Additional Total RNA Extraction 160-001-162
calculated with the use of the proprietary miRXplore
* PIQOR™ Microarray Kits are not available in the US and Canada. Universal Reference.
** Microarray Service includes all experimental steps from RNA isolation to
primary data analysis. Final data are returned including an extensive written
report. Further Bioinformatics Services, such as pathway or cluster analysis, are Reference
also available.
*** In combination with the Microarray Services only. The SuperAmp Service 1. Landgraf, P. et al. (2007) Cell 129: 1401–1414.
is not available for microRNA amplification
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