This document discusses optimizing product performance through innovative formulations and cutting-edge technology. It provides examples using Pronamel toothpaste and mouthwash, which maximize available fluoride levels through careful ingredient selection like sodium fluoride and polymers. New technologies like DSIMS and light interferometry were used to study fluoride penetration into enamel and reduce erosion. Together, innovative formulations and technologies allow creating high performing products that deliver targeted benefits like reducing dental erosion.

1. Optimising product performance through the combination of innovative formulations and cutting edge technology InnoCosmetics Europe Thursday 7th October 2010 Optimising product performance through the combination of innovative formulations and cutting edge technology InnoCosmetics Europe Thursday 7th October 2010 Dr Mark Edwards Director Biosciences and Idea Evolution, GSK Consumer Healthcare Dr Mark I Edwards Director Biosciences and Idea Evolution, GSK Consumer Healthcare

3. Demonstrate the use of cutting edge technology to driving new product development and formulation

5. Dental Erosion Formulation Goal – To Reduce Enamel & Dental Erosion Intake of acidic food or drink Enamel loses mineral content and becomes surface softened Saliva neutralizes and slowly replaces mineral Abrasion e.g. brushing & Attrition e.g. tooth grinding result in enamel loss Once enamel is lost it can not be replaced However frequent attack by dietary acids leads to significant surface softening Dental erosion is commonly defined as the chemical dissolution of dental hard tissues by methods other than those involving bacterial acid production, the role of dietary acids as a major contributor to this process is well established 1,2. Enamel is the hard, protective coating of the tooth, when the enamel is worn away, the dentine underneath is exposed, which may lead to pain and sensitivity. 1. Zero DT, Lussi A: Erosion – chemical and biological factors of importance to the dental practitioner. Int Dent J 55:285-290, 2005. 2. Lussi A. (Ed.) Dental Erosion: From Diagnosis to Therapy. Monographs in Oral Science Vol 20. Pub: Karger, Basel, 2006.

6. Optimising Product Performance Pronamel does this by maximising the ‘free’ or available fluoride in the formulation. This is important because: Tooth enamel is composed of hydroxyapatite (HAP), Ca10(PO4)6(OH)2 Fluoride treatment creates fluorapatite Ca10(PO4)6F2 Fluorapatite is harder and more resistant to acid attack that HAP. The greater the amount and depth of fluorapatite formed the more resistant the tooth is to enamel softening and loss on exposure to dietary acids.

9. DSIMS - Dose Response Study

10. Formulation –The Effect of Different Fluoride Salts on Fluoride Penetration into Enamel using DSIMS All fluoride containing toothpastes leads to fluoride penetration and incorporation into the surface of enamel. Fluoride penetration was significantly greater (49-150%) for pastes containing sodium fluoride alone vs. those based on Amine Fluoride, Stannous Fluoride or Sodium Fluoride in combination with pyrophosphate. B: Paste 2 treated (NaF + Sodium Pyroposphate) A: Paste 1 treated (NaF) C: Paste 3 treated (NaF + KNO3) D: Paste 4 treated (SnF2) E: Paste 5 Treated (AmF) Poster Presentation: International Association Dental Research, General Meeting, New Orleans, March 21st - 24th 2007

12. The degree of fluoride incorporation into enamel corresponded with the extent of rehardening of surface softened enamel.Commercial toothpaste New Formulation toothpaste * Micron = one millionth of a meter Nanometer= one billionth of a meter Overlay Image for new test desensitising toothpaste (F- red, C2H- blue) Fluoride-free Control toothpaste Poster Presentation: IADR Pan European Federation Meeting, Dublin , 2006

13. Incorporating polymers optimise product performance in a mouthwash formulation Maximises the ‘free’ or available fluoride in the formulation Tri-Hydra™ a three polymer system - xanthan gum, carboxymethyl cellulose, copovidone These polymers provide greater enamel fluoride uptake Ingredients: aqua, glycerin, sorbitol, poloxamer3381, PEG-60 hydrogenated castor oil, VP/VA copolymer2, potassium nitrate, sodium benzoate, cellulose gum, aroma, sodium fluoride, methylparaben, propylparaben, cetylpyridinium chloride, sodium saccharin, xanthan gum, disodium phosphate, sodium phosphate, CI 42090, sodium fluoride 1. Polyethylene-Polypropylene Glycol 2. Polyvinylpyrrolidone/Vinyl Acetate (VP/VA) Copolymer

14. DSIMS to Measure Enhancement of Fluoride by the Three Polymer System 16O- 19F- 12C21H 31P Fluoride layer 300 ppm F + 3 Polymer System 300 ppm F Distance (µm) Distance (µm)

15. Optimising product performance through cutting edge technology - White Light Interferometry Non Contact Surface Profiling Quantitative assessment of shape, texture and topography (nm height resolution) Enables acid based enamel surface damage/damage prevention to be determined

16. White Light Interferometry - Untreated Human Enamel

17. White Light Interferometry - Human Enamel Treated with 1% Citric Acid pH3.8 for 5 Minutes

18. White Light Interferometry to Measure Erosion Inhibition 3 Polymer System Water 300ppm F + 3 Polymer System 300ppm F Poster Presentation: International Association Dental Research, General Meeting Miami Florida, April 1-4 2009

19. Publications:

21. The use of new technology to driving new product development and formulation has been shown by:

22. DSIMS

23. White light inferometry

24. Tri-Hydra™ three polymers system used in Pronamel mouthwash

25. Sodium fluoride: an example showing the importance of choosing the right ingredients for the targeted benefit

27. Vielen DankThank you

28. Questions?

29. Carbopol Xanthan Sodium Lauryl Sulphate TegoBetaine