Saravanan Kumar,V.Siva Reddy - Workshop on Proteomics
1. THE SCIENCE BEHIND MASS SPECTROMETRY (Biology of a Chemistry Machine) SARAVANAN KUMAR , Research Associate , Proteomics Facility, Plant Transformation Group, International Centre for Genetic Engineering & Biotechnology, New Delhi National Agricultural Innovation Project (NAIP) supported workshop on Proteomics: High Throughput Analysis of Proteins and Proteome by Mass Spectrometry” 22 nd - 26 th March 2010 ICGEB, New Delhi DR. V. SIVA REDDY , Organizer & Consortium Principal Investigator , NAIP component -4 , International Centre for Genetic Engineering & Biotechnology, New Delhi
4. Mass Spectrometry/meter..... Mass spectrometry – Science – based on the motion of charged particles in a electric or magnetic field Motion- movement- mass/charge (m/z) z- if the charge is known, the mass of the ion is known m/z – m + or (m+H) + m/z= (M+n) /n M= (m/z)*n-n Transfers sample in to vacuum Ionization/ acceleration Flight/ resolution Signal detection
5. Inside the MS meter ,Ionisation.... ESI- Electro spray ionisation LDI –Laser desorption ionisation
35. You need to be aware of what others are doing, applaud their efforts, acknowledge their successes, and encourage them in their pursuits. When we all help one another, everybody wins.