iGEM 2013 - Brazil_UFMG team http://bios.ligamedica.com

2. Fernando
Liza Santuza Omar
Alan Júlio
Paola
Lucas
ÍtaloRaphael
Carlos
Marianna
PriscilaClara
Our team

3. Minas Gerais, Brazil
Where we came from

4. Where we came from
Belo Horizonte - MG

5. Where we came from
Universidade Federal de Minas Gerais (UFMG)

6. Acute Coronary Syndrome (ACS)
NIH Medline Plus
Winter 2009 Issue: Volume 4 Number 1
A worldwide problem

7. Acute Coronary Syndrome (ACS)A worldwide problem
WHO, 2013

8. Acute Coronary Syndrome (ACS)A Brazilian problem
Teich et al., Rev Bras Cardiol., 2011

9. Acute Coronary Syndrome (ACS)A challenge

10. • To develop a sensitive device capable of
detecting and integrating the signals of ACS
biomarkers, setting a prognosis.
Aiming high

11. Biomarkers

12. Tang et al., N Engl J Med., 2013
Trimethylamine-N-OxideTMAO: Introduction

13. ‘
Trimethylamine-N-OxideTMAO: Construction
TorS
TorT
TMAO
TorT
TorR
TMAO
TorR*
pTorCAD RFP
BBa_K1086000-------- Ba_K1086002 -------

14. ‘
Trimethylamine-N-OxideTMAO: Construction
TorS
TorT
TMAO
TorT
TorR
TMAO
TorR*
pTorCAD RFP
TorT + TMAO
2 TorT + 2 TMAO + TorS

15. Trimethylamine-N-OxideTMAO: Modeling

16. Trimethylamine-N-OxideTMAO: Results
TorCAD+RFP ----- TMAO
Fluorescence/Absorbance

17. Ischemia Modified AlbuminIMA: Introduction
Normal patient blood
(Cobalt added)
Ischemic patient blood
(Cobalt added)
Albumin
IMA

18. Ischemia Modified AlbuminIMA: Construction
pRCNA YFP
-------- Ba_K1086001 --------
Normal patient blood
(Cobalt added)
Ischemic patient blood
(Cobalt added)
Albumin
IMA

19. Ischemia Modified AlbuminIMA: Modeling

20. Ischemia Modified AlbuminIMA: Modeling
Albumin 90 µM

21. Ischemia Modified AlbuminIMA: Modeling
Cobalt 25 µM

22. Ischemia Modified AlbuminIMA: Results
RCNA+YFP ----- Cobalt
Free Cobalt Assay:
Fluorescence/Absorbance

23. Ischemia Modified AlbuminIMA: Results
Bovine Serum Albumin Assay:
Fluorescence
RCNA+YFP ----- Cobalt

24. Ischemia Modified AlbuminIMA: Results
RCNA+YFP ----- IMA+Cobalt
Ischemic and Non-Ischemic Mice Sera Assay:
Fluorescence
Non-Ischemic
Ischemic

25. Talking with specialistsHuman Practices
Doctor Antonio Luiz Pinho Ribeiro
ETHICS BIOSAFETY
Neuza Antunes Rodrigues

26. Interest over time. Web Search. Worldwide, 2004 - present.
Scientific disseminationHuman Practices
Interest over time. Web Search. Worldwide, 2004 – present
English x Portuguese

27. GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination

28. Scientific disseminationHuman Practices
Our team

29. Scientific disseminationHuman Practices
Our team
Our university

30. Scientific disseminationHuman Practices

31. Scientific disseminationHuman Practices
Our team
Our university
Public schools

32. Scientific disseminationHuman Practices
UFMG & Escolas

33. Scientific disseminationHuman Practices
Missions
1. Fuel from sunlight
2. Microplastic hazard on the oceans
3. Spoiled meat
4. Malaria and artemisin
5. Celiac disease
6. Space exploration
7. Our own project
The Brickard Game

34. Scientific disseminationHuman Practices
Our team
Our university
Public schools
Our state

35. Scientific disseminationHuman Practices

36. Scientific disseminationHuman Practices
Our team
Our university
Public schools
Our state
Brazil

37. Scientific disseminationHuman Practices
Website

38. Scientific disseminationHuman Practices
Wikipedia
facebook.com/igemufmg
twitter.com/igemufmg

39. Scientific disseminationHuman Practices
Video about
Synthetic Biology
Newspaper

40. GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination

41. Thinking outside the boxHuman Practices
Hackspace

42. Thinking outside the boxHuman Practices
Our song!

43. GoalsHuman Practices
Cardbio
Impact
Thinking
outside
the box
Talking
with
specialists
Collaboration
Scientific
dissemination

44. CollaborationHuman Practices

45. CollaborationHuman Practices
Jamboré – Meeting of Brazilian teams

46. Achievements
 Assembly, registry and characterization of new biobricks:
 RCNA + YFP (BBa_K1086001)
 TorCAD (BBa_K1086000)
 TorCAD+ RFP (BBa_K1086002)
 Modeling of our systems.
 Expand the knowledge about synthetic biology in our University,
our State and our Country.


47. Thanks
Sponsors
Other supporters
Laboratório de Genética Bioquímica
Laboratório de Sinalização Celular e
Nanobiotecnologia
Laboratório de Genômica e Genética de Parasitos
Laboratório de Imunoquímica de Toxinas Naturais
Laboratório de Sistema de Informação
Laboratório de Angiogênese
Professor Gastone Castellani
Professora Andréa Mara Macedo
Neuza Antunes Rodrigues
Juliana Pimenta
Rosalvo Fogaça –UFPR
Izabella Pena Neshich – UNICAMP
Doutor Antonio Luiz Pinho Ribeiro
André Luis Martins Reis – Johns Hopkins

48. Thanks
Crowd funding donators
Denis Roger
Pedro Henrique Nascimento de Aguiar
Filipe Silva Villela
Sabrina Lima
Marina Diniz
Diego Pessoa Santos
Graciela Kunrath Lima
Bel Bitar
Isabela F. Prates
Marcela Segatto do Carmo
Henrique Teichmann
Nicole Scherer
Wagner Arbex
Glória Franco
Naíla Barbosa da Costa
Fernanda Santos Freitas
Pedro E. C. Vieira
Francislon Silva de Oliveira
Lucas Antunes de Siqueira Costa
Pedro Pinho Teixeira
Jarbas Rohr
Mainá Bitar
Leonardo Lucas Carnevalli Dias
Luandy Baião
Wesley Júnio da Silva
Michele Araújo
Neuza Antunes
Juliano Leal
Ceres Luciana Alves
Maíra Freire
Amanda Campelo
Thank you!