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Uncovering the Mysteries
of Umami and MSG
Lisa Watson
The Glutamate Association
Show of Hands
Nutri&on	
  
Food	
  
Safety	
  TASTE!	
  
Christopher Koetke, MBA, CEC, CCE
Vice President Culinary Arts
Kendall College
A Little Help from my Friend
On “Believing”
§  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE
Now, interestingly enough, I haven’t always believed in umami. In fact, I, like a
lot of people, were sort of on the fence if it really existed, and certainly I didn’t
want to use MSG. But one day in one of my classes I was teaching, I
remember talking with a student from the Philippines, and in – in the lecture
that day I had mentioned about how we don’t use MSG in our kitchen because
it’s a little suspect or whatever. And afterwards, the student came up to me and
she said, you know chef, I don’t get it, what is it about MSG, because when I’m
at home, my mom has it in the kitchen and we grew up eating it on a lot of
things. Now all of a sudden I’ve sort of got this question mark in my head. And
then she says, chef, have you ever tried it on scrambled eggs? And so right
then in the kitchen we made some scrambled eggs, ‘cause I was curious, and
she put in the MSG and I couldn’t believe the flavor boost. And it was that day
that I started thinking, you know, maybe there’s something to this umami thing.
Ø  View video online here: http://youtu.be/PLgKDLqL9Mc
Today’s Umami Journey
Umami—does it really exist? What does it taste like?
MSG and American food culture/lore
Foundation on glutamate for critical thinking
Glutamate and synergistic compounds in food
How is Food Interpreted
Through a process of physiological stimuli
and environmental factors
And each has its reason for existing
Detection Levels for
Basic Tastes
What is Umami?
§  Start with definitions
Ø  Glutamic acid = glutamate = One of 20 amino acids
(protein)
Ø  Free glutamic acid = one which is not bound to anything
else
Ø  Umami = taste sensation derived from free glutamic
acid
Ø  Ribonucleotide = molecule that boosts synergistically
umami flavor profile
History of Umami
§  Discovered and characterized by Professor K. Ikeda, 1908
§  Specific to “savory taste” of L-glutamate
§  Focus by public only fairly recent
Glutamate sources around the world
Where IS Umami Found?
§  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE
So where do we find this umami flavor profile? In the kitchen it’s in a lot of
different places. It comes in fermented sauces like soy sauce and fish sauce or
maybe as a paste like miso, for instance, or in aged cheeses and in aged
hams, maybe in ripe fruits like tomato. You find umami in a lot of places, and
what means is that inside that food you have that free glutamic acid combining
with the sodium and voila, you have umami. Now you can also get this umami
flavor sensation through the use of MSG. And MSG is the purest form of the
umami flavor. Originally, it was derived from kambu or that big seaweed that
grows especially off the coast of Japan, and, in fact, that’s how umami was
discovered by taking the roth or dashi, that’s made from kambu and bonito
flakes, and reducing it down until it’s crystal in form, and that’s the discovery of
MSG.
Ø  View video online here: http://youtu.be/2zuayLvO-wQ
And It Is Complicated…But REAL
To Brain
R
Ca2+
Store
[Ca2+]i
L-glutamate
GMP
IMP
G
2nd Messengers
G Protein-Coupled
Receptors
Na
TrpM5
++
ATP
T1R2 + T1R3
2000:
Specific
L-glutamate
taste
receptor
discovered.
“Venus Flytrap” Mechanism
Zhang et al., PNAS 106 (2008)
Background on Glutamate
Glutamic Acid in the Body
n  One of 20 amino acids needed for life
n  Most prevalent amino acid in the body
n  We consume 10-20 g glutamic acid daily
n  Our bodies manufacture about 50g per
day
n  Key neurotransmitter in the brain
n  Approximately 96% of dietary glutamate
used by gut mucosal cells as energy
source
Glutamic Acid in the Body
n  18 times more glutamate in human milk vs. cow
milk
	
  
GLUTAMATE IN FOODS
Enhancing Umami Taste Correlates with
Increasing Glutamate Concentration
§  Ripening
§  Maturation
§  Curing
§  Cooking
§  Adding
glutamate or
glutamate-rich
stock
Free vs. Total Glutamate (mg/100g)
Total Free
Cheddar cheese 6090 600
Parmesan cheese 8210 1600
Tomato 310 246
Corn 650 106
Umami receptors
detect free
glutamate.
Includes free
glutamate plus that
“bound” to protein
Ribonucleotide Synergy: Up to15-fold!
Zhang et al., PNAS 106 (2008)
Ribonucleotide Synergy:
The age-old wisdom of chefs
§  5-Inosinate—meat based (i.e.
sardines, mackerel, tuna, pork, beef
and chicken)
§  5-Guanylate—plant based (i.e.
mushrooms)
§  The magic of umami synthesis
Ø  1 + 1 = 4 or even 15!
Monosodium Glutamate
§  Sodium salt of glutamic acid
§  Favorable flavor profile compared to most other salts
of glutamic acid
§  Body recognizes it identically to free glutamic acid in
food
§  Good shelf stability
§  Recognized flavor enhancer
§  Common food additive
Making Glutamate Seasoning
Sugar
Fermentation
Separate/
Crystalize
Dry
Daily Consumption (mg) of Monosodium Glutamate
(Used as Flavor Enhancer) in Selected Countries
0	
  
500	
  
1000	
  
1500	
  
2000	
  
2500	
  
3000	
  
3500	
  
U.S.*	
   U.K.*	
   Japan**	
   Korea**	
   Taiwan	
  
* Assumes 70 kg individual
** Assumes 60 kg individual
MSG Role in the Kitchen
§  Creates more fully rounded
flavor profile
§  Pure form of umami for
increasing umami without
additional flavor profiles
§  Useful in diets to reduce salt
(70% less sodium per teaspoon
than table salt--640 mg and
2,300 mg)
§  Useful in diets to reduce fat
The Elephant in the Room:
§  1958 US FDA—Generally Recognized as Safe
(GRAS)
§  1987 Joint Expert Committee on Food Additives
(JECFA) of UN Food and Agriculture Organization
confirms MSG is safe
§  1991 European’s Commission’s Scientific Committee
for Food decides unnecessary to set Acceptable Daily
Intake level (ADI)
§  1995 US FDA Federation of American Societies for
Experimental Biology (FASEB) confirms safety as
food ingredient
Is it safe?
But What About . . .
How I usually respond to questions . . .
§  Always respect what others feel and experience
§  Share basic information about glutamate and the
diet
§  Share basic information about safety studies
§  Stick with the facts – not my place to convince!
Final Thoughts from Chef Chris
§  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE
So, you’ve learned about what this umami sensation is, you’ve learned
about where it comes from and how it’s made by different molecules
like ribonucleotides acting synergistically with that glutamate molecule
or the sodium and free glutamic acid, you know that. And you also
know that it’s a perfectly safe molecule to use in cooking.
Now, the next step is for you. Go in the kitchen and try it, put some
MSG on different products, put it into eggs, put it into soup and watch
what happens to the flavor perception, watch what happens to the
entire level of the flavor in the food. Give it a try, experiment, and I
think you’ll be surprised, happily surprised, with what you see.
Ø  View video online here: http://youtu.be/y5CcGwNixj0
Thank you!
www.msgfacts.com
www.umamiinfo.com
www.glutamate.org

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Uncovering the Mysteries of MSG and Umami

  • 1. Uncovering the Mysteries of Umami and MSG Lisa Watson The Glutamate Association
  • 3. Nutri&on   Food   Safety  TASTE!   Christopher Koetke, MBA, CEC, CCE Vice President Culinary Arts Kendall College A Little Help from my Friend
  • 4. On “Believing” §  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE Now, interestingly enough, I haven’t always believed in umami. In fact, I, like a lot of people, were sort of on the fence if it really existed, and certainly I didn’t want to use MSG. But one day in one of my classes I was teaching, I remember talking with a student from the Philippines, and in – in the lecture that day I had mentioned about how we don’t use MSG in our kitchen because it’s a little suspect or whatever. And afterwards, the student came up to me and she said, you know chef, I don’t get it, what is it about MSG, because when I’m at home, my mom has it in the kitchen and we grew up eating it on a lot of things. Now all of a sudden I’ve sort of got this question mark in my head. And then she says, chef, have you ever tried it on scrambled eggs? And so right then in the kitchen we made some scrambled eggs, ‘cause I was curious, and she put in the MSG and I couldn’t believe the flavor boost. And it was that day that I started thinking, you know, maybe there’s something to this umami thing. Ø  View video online here: http://youtu.be/PLgKDLqL9Mc
  • 5. Today’s Umami Journey Umami—does it really exist? What does it taste like? MSG and American food culture/lore Foundation on glutamate for critical thinking Glutamate and synergistic compounds in food
  • 6. How is Food Interpreted Through a process of physiological stimuli and environmental factors And each has its reason for existing
  • 8. What is Umami? §  Start with definitions Ø  Glutamic acid = glutamate = One of 20 amino acids (protein) Ø  Free glutamic acid = one which is not bound to anything else Ø  Umami = taste sensation derived from free glutamic acid Ø  Ribonucleotide = molecule that boosts synergistically umami flavor profile
  • 9. History of Umami §  Discovered and characterized by Professor K. Ikeda, 1908 §  Specific to “savory taste” of L-glutamate §  Focus by public only fairly recent
  • 11. Where IS Umami Found? §  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE So where do we find this umami flavor profile? In the kitchen it’s in a lot of different places. It comes in fermented sauces like soy sauce and fish sauce or maybe as a paste like miso, for instance, or in aged cheeses and in aged hams, maybe in ripe fruits like tomato. You find umami in a lot of places, and what means is that inside that food you have that free glutamic acid combining with the sodium and voila, you have umami. Now you can also get this umami flavor sensation through the use of MSG. And MSG is the purest form of the umami flavor. Originally, it was derived from kambu or that big seaweed that grows especially off the coast of Japan, and, in fact, that’s how umami was discovered by taking the roth or dashi, that’s made from kambu and bonito flakes, and reducing it down until it’s crystal in form, and that’s the discovery of MSG. Ø  View video online here: http://youtu.be/2zuayLvO-wQ
  • 12. And It Is Complicated…But REAL To Brain R Ca2+ Store [Ca2+]i L-glutamate GMP IMP G 2nd Messengers G Protein-Coupled Receptors Na TrpM5 ++ ATP T1R2 + T1R3 2000: Specific L-glutamate taste receptor discovered.
  • 13. “Venus Flytrap” Mechanism Zhang et al., PNAS 106 (2008)
  • 15. Glutamic Acid in the Body n  One of 20 amino acids needed for life n  Most prevalent amino acid in the body n  We consume 10-20 g glutamic acid daily n  Our bodies manufacture about 50g per day n  Key neurotransmitter in the brain n  Approximately 96% of dietary glutamate used by gut mucosal cells as energy source
  • 16. Glutamic Acid in the Body n  18 times more glutamate in human milk vs. cow milk  
  • 18. Enhancing Umami Taste Correlates with Increasing Glutamate Concentration §  Ripening §  Maturation §  Curing §  Cooking §  Adding glutamate or glutamate-rich stock
  • 19. Free vs. Total Glutamate (mg/100g) Total Free Cheddar cheese 6090 600 Parmesan cheese 8210 1600 Tomato 310 246 Corn 650 106 Umami receptors detect free glutamate. Includes free glutamate plus that “bound” to protein
  • 20. Ribonucleotide Synergy: Up to15-fold! Zhang et al., PNAS 106 (2008)
  • 21. Ribonucleotide Synergy: The age-old wisdom of chefs §  5-Inosinate—meat based (i.e. sardines, mackerel, tuna, pork, beef and chicken) §  5-Guanylate—plant based (i.e. mushrooms) §  The magic of umami synthesis Ø  1 + 1 = 4 or even 15!
  • 22. Monosodium Glutamate §  Sodium salt of glutamic acid §  Favorable flavor profile compared to most other salts of glutamic acid §  Body recognizes it identically to free glutamic acid in food §  Good shelf stability §  Recognized flavor enhancer §  Common food additive
  • 24. Daily Consumption (mg) of Monosodium Glutamate (Used as Flavor Enhancer) in Selected Countries 0   500   1000   1500   2000   2500   3000   3500   U.S.*   U.K.*   Japan**   Korea**   Taiwan   * Assumes 70 kg individual ** Assumes 60 kg individual
  • 25. MSG Role in the Kitchen §  Creates more fully rounded flavor profile §  Pure form of umami for increasing umami without additional flavor profiles §  Useful in diets to reduce salt (70% less sodium per teaspoon than table salt--640 mg and 2,300 mg) §  Useful in diets to reduce fat
  • 26. The Elephant in the Room: §  1958 US FDA—Generally Recognized as Safe (GRAS) §  1987 Joint Expert Committee on Food Additives (JECFA) of UN Food and Agriculture Organization confirms MSG is safe §  1991 European’s Commission’s Scientific Committee for Food decides unnecessary to set Acceptable Daily Intake level (ADI) §  1995 US FDA Federation of American Societies for Experimental Biology (FASEB) confirms safety as food ingredient Is it safe?
  • 27. But What About . . .
  • 28. How I usually respond to questions . . . §  Always respect what others feel and experience §  Share basic information about glutamate and the diet §  Share basic information about safety studies §  Stick with the facts – not my place to convince!
  • 29. Final Thoughts from Chef Chris §  TRANSCRIPT: VIDEO CLIP OF CHRIS KOETKE So, you’ve learned about what this umami sensation is, you’ve learned about where it comes from and how it’s made by different molecules like ribonucleotides acting synergistically with that glutamate molecule or the sodium and free glutamic acid, you know that. And you also know that it’s a perfectly safe molecule to use in cooking. Now, the next step is for you. Go in the kitchen and try it, put some MSG on different products, put it into eggs, put it into soup and watch what happens to the flavor perception, watch what happens to the entire level of the flavor in the food. Give it a try, experiment, and I think you’ll be surprised, happily surprised, with what you see. Ø  View video online here: http://youtu.be/y5CcGwNixj0