This document contains images and descriptions of abnormal sperm cells from bulls, including double headed sperm, misshapen heads, elongated heads, pear-shaped heads, detached heads, bent tails, coiled tails, and proximal and distal droplets. The document also contains images of developing dog and bull embryos at various stages from a single cell to a hatching blastocyst.

2. Blood in a canine ejaculate (hemospermia(

3. Semen samples from a bull (left( and dog (right(, showing differences in opacity and
concentration

9. Double headed sperm (bull;
eosin-nigrosin stain(
Misshapen head along with 4 normal sperm
(bull; toluidine blue stain(

10. Elongaged head (bull; eosin-nigrosin stain( Elongated head (bull; toluidine blue stain(

11. Pyriform (pear-shaped) head and bent,
abnormal midpiece (bull; eosin-nigrosin stain)
Misshapen head and proximal
droplet (bull; toluidine blue stain)

12. Proximal droplet (bull; toluidine blue stain) Distal droplet (bull; toluidine blue stain)

13. Detached head (bull; eosin-nigrosin stain) Bent tail or midpiece (bull; eosin-nigrosin stain)

14. Coiled tail (bull; eosin-
nigrosin stain)
Coiled midpiece/tail in one sperm and proximal
droplet in another (bull; eosin-nigrosin stain)

23. Bent or reversed tail

24. Tightly coiled tail

25. Detatched head

26. Wrinkled acrosome - this
may reflect a nuclear
problem which prevents
zona attachment by the
sperm cell. It is a rare
condition.

29. Micromanipulator

30. Expanded blastocyst

31. CIDAR

32. Blastocyst

33. Collected COCs

34. 4Cell embryo

35. Compact morula

36. 8Cell Embryo

37. Hatching blastocyst

38. One cell embryo with 2 pronuclei

39. ICSI

40. 2cell Embryo