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The Cycle
Bromelain
Papain
i.     Area of Industry                    ii.        Enzyme                iii.          Application
    iv.        Baking Industry               v.            Alpha-amylase &      vi.            To break down starch
                                                           Proteases                 to sugar in flour and to lower
                                                                                         the protein level of flour
   vii.        Biofuel                       viii.            Cellulases &            ix.         To break down
                                                           ligninases                cellulose into sugars so they
                                                                                             can be fermented
    x.         Infant Foods                          xi.        Trypsin           xii.           To predigest baby
                                                                                                   foods
  xiii.        Brewing                        xiv.        Amylases,             xv.             To convert starch to
                                                glucanases, protases,                       simple sugar, split
                                                   betaglucosidase,                    polysaccharides into malt,
                                                   amyloglucosidase                  improve filtration, production
                                                                                         of low-cal beer, remove
                                                                                                cloudiness
  xvi.         Fruit Juices                  xvii.             Cellulases,     xviii.           To clarify fruit juices
                                                           pectinases
  xix.         Dairy                     xx.                Rennin, lipases,   xxi.       Hydrolising protein in
                                                            lactases                 cheese manufacturing,
                                                                               ripening cheese and breaking
                                                                                    down lactose to glucose
  xxii.        Detergent            xxiii.              Proteases, amylases,xxiv.          Pre-soaks, remove
                                                     lipases, cellulases          starch residues in machine
                                                                                dishwashing, removal of fatty
                                                                               and oil stains, biological fabric
                                                                                          conditioners
  xxv.         Meat                               xxvi.        Papain       xxvii.         To tenderize meat
xxviii.        Paper & Pulp         xxix.               Amylases, xylanases, xxx.          Degrade starch to
                                                   Cellulases, ligninases       lower viscosities to aid sizing
                                                                                   and coating paper, reduce
                                                                                       bleach required for
                                                                                   discolouring, soften paper
xxxi.
i.       Enzyme        ii.         Source                iii.     Application
       Catalase               iv.           Chicken Livers         v.       Food Processing
   vi.         Lipase         vii.          Cow pancreas        viii.       Food Processing
       Actinidin                    ix.        Kiwi fruit          x.       Food Processing
       Amylase                xi.          Barley, bacillus,   xii.        Brewing, starch and
                                          aspergillus                        baking
     Bromelain                 xiii.         Pineapples               xiv.      Brewing
     Ficin                            xv.        Figs           xvi.        Food Processing
     Lipoxygenase              xvii.          Soybeans         xviii.       Food Processing
     Papain                        xix.        Papaya            xx.         Meat tenerising
     Protease                     xxi.         Bacillus             xxii.      Detegents
xxiii.
Energy
                             Basically: good smell good microbes, bad smell bad
                                                  microbes.



                         Microbes                             Can identify and measure
                                                                 enzymes through
                                                                spectrophotometer
         Enzymes start
          forming here


                                    Enzymes

                                                                       Plus
                                                                  Other enzymes,
                                                                  amino acids, etc.

                                                Can measure reaction through brix
                                                      level (refractometer)



                                        Glucose




                                                                        Time
papain
                    bromelain




microbes


Enzyme s
Coctail of enzymes, microbes
                                bromelain




microbes
microbes
  microbes
                       crobes
Glucose consumption
                         Microbial growth




                      Enzyme Production




                             Time/days 
Formula I: A General Enzyme Base for
                  Cosmetic Products
Banana, grape, Pineapple, apple,       6 kilograms in various proportions
orange, papaya, Mango stein            according to person’s own formula
Honey                                  2 liters
Water                                  20 Liters
Procedure: Place all ingredients
together in a sealed tank and mix.
Leave for at least one year. This
process can be sped up by placing an
existing enzyme in the mixture.

                                         Hunter & Yothangrong (2006)
Formula 4: Dishwashing Liquid

Enzyme (base) Base will consist   15 Kilograms
heavily of citrus fruits
Coconut oil or CDE                2 kilogram
Ash water                         15 kilograms
Sodium         Ammonian         MonoEA           DEA            TEA
Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate

                             Solubility Increases

                             Viscosity Decreases

                           Cold Storage Improves

                             Mildness Increases

                               Cost Increases



            Use as an ingredient in standard
                     formulations
Attributes        Generics               Discount                Lower             Middle          Leader         Pharmac
                                                                                                                      y
   Solids          5-15%                  5-25%                  10-30%            10-35%          25-40%           25-40%
 Level
    Primary             SLS                  SLS/ALS              ALS/              ALS/            DEA LS            DEA LS
   Surfactant           SLES                  SLES                MEA LS            MEA LS          TEA LS            TEA LS


   Secondary       Alkanolamides         Alkanolamides         Alkanolamides       Alkyl (amido)   Alkyl (amido)   Alkyl (amido)
   Surfactant                                                   Alkyl (amido)       Betaines &      Betaines &      Betaines &
                                                              Betaines & Amine     Amine Oxides    Amine Oxides    Amine Oxides
                                                                   Oxides



Pacifier/Visc.   Sodium Chloride       Sodium Chloride       Sodium Chloride           Glycol          Glycol          Glycol
                                                                                     Stearates       Stearates       Stearates
    Control       Ethylene Glycols       Ethylene Glycols      Ethylene Glycols



  Functional      Maybe in ascetic       Maybe in ascetic      Maybe in ascetic     Number of       Number of       Number of
  Ingredients    quantities             quantities            quantities             choices         choices         choices
                                                                 Enzymes here

  Fragrance      Low cost selections   Low cost selections   Low cost selections    Higher cost     Higher cost     Higher cost
                                                                                    selections      selections      selections




 Preservatives       Formalin               Formalin         Sodium Benzoate        Number of       Number of       Number of
                                                                                     choices         choices         choices
Shampoo System profile (what everything does)
     Function                      Potential Material                                  Objective
Primary surfactant       ALS, ALES, TLS, SLES, NaDOBS                          Performance, strength,
                                                                               efficacy
 Secondary surfactant/   Coconut MEA, Cocamide MEA, Cocamidopropyl             Tactile and sensory
                                                                               perception, impact, theme
Foam Stabiliser          Betaine or amine
                                                                               ingredients, efficacy

   Pacifier/Pearliser    Ethylene Glycol Distearate, Glycol Stearate           Tactile and sensory
                                                                               perception, general
                                                                               impression, impact
Viscosity Control        Sodium chloride or gums                               Ingredients, general
                                                                               impression, strength
     Preservatives       Potassium Sorbate, Diazolidonyl urea, Methyl and      Ingredients, general
                                                                               impression
                         Propyl Parabens
Functional Additives     Guar, Hydroxypropyl Trimmonium Chloride               Tactile and sensory
                                                                               perception, general
                                              Panthenol, Dimethicone,
                                                                               impression, spiritual
                         Hydrolysed Keratin Protein                            impression, impact, efficacy,
                                           Enzymes here                        intangible benefits, signal
                                                                               attributes

    Fragrance            Primarily aroma chemicals that are synthesized        General impression, odour
                                                                               profile, impact, efficacy,
                         used in conventional products, or natural essential
                                                                               intangible benefits, signal
                         oils that become part of the active ingredients       attributes

Ascetic Ingredients      Aloe Vera, Green Tea Extract, Panthenol               General impression,
                                                                               intangible benefits, tactile
                                                                               and sensory perception,
                                                                               impact, signal attributes

         Dyes            Mainly synthetic, more naturals                       General impression, signal
                                                                               attributes
Auxiliary cleaner
            Anti dandruff
Protein stain remover (mouthwash)
           Teeth whitener
         Skin whitener???
            Skin softener
       Grease & oil remover
  Dead skin remover (anti aging)
          Odour remover
            Antiseptic???

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Making Basic Enzymes

  • 1.
  • 5. i. Area of Industry ii. Enzyme iii. Application iv. Baking Industry v. Alpha-amylase & vi. To break down starch Proteases to sugar in flour and to lower the protein level of flour vii. Biofuel viii. Cellulases & ix. To break down ligninases cellulose into sugars so they can be fermented x. Infant Foods xi. Trypsin xii. To predigest baby foods xiii. Brewing xiv. Amylases, xv. To convert starch to glucanases, protases, simple sugar, split betaglucosidase, polysaccharides into malt, amyloglucosidase improve filtration, production of low-cal beer, remove cloudiness xvi. Fruit Juices xvii. Cellulases, xviii. To clarify fruit juices pectinases xix. Dairy xx. Rennin, lipases, xxi. Hydrolising protein in lactases cheese manufacturing, ripening cheese and breaking down lactose to glucose xxii. Detergent xxiii. Proteases, amylases,xxiv. Pre-soaks, remove lipases, cellulases starch residues in machine dishwashing, removal of fatty and oil stains, biological fabric conditioners xxv. Meat xxvi. Papain xxvii. To tenderize meat xxviii. Paper & Pulp xxix. Amylases, xylanases, xxx. Degrade starch to Cellulases, ligninases lower viscosities to aid sizing and coating paper, reduce bleach required for discolouring, soften paper xxxi.
  • 6. i. Enzyme ii. Source iii. Application Catalase iv. Chicken Livers v. Food Processing vi. Lipase vii. Cow pancreas viii. Food Processing Actinidin ix. Kiwi fruit x. Food Processing Amylase xi. Barley, bacillus, xii. Brewing, starch and aspergillus baking Bromelain xiii. Pineapples xiv. Brewing Ficin xv. Figs xvi. Food Processing Lipoxygenase xvii. Soybeans xviii. Food Processing Papain xix. Papaya xx. Meat tenerising Protease xxi. Bacillus xxii. Detegents xxiii.
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  • 10. Energy Basically: good smell good microbes, bad smell bad microbes. Microbes Can identify and measure enzymes through spectrophotometer Enzymes start forming here Enzymes Plus Other enzymes, amino acids, etc. Can measure reaction through brix level (refractometer) Glucose Time
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  • 13. papain bromelain microbes Enzyme s
  • 14. Coctail of enzymes, microbes bromelain microbes microbes microbes crobes
  • 15. Glucose consumption Microbial growth Enzyme Production Time/days 
  • 16. Formula I: A General Enzyme Base for Cosmetic Products Banana, grape, Pineapple, apple, 6 kilograms in various proportions orange, papaya, Mango stein according to person’s own formula Honey 2 liters Water 20 Liters Procedure: Place all ingredients together in a sealed tank and mix. Leave for at least one year. This process can be sped up by placing an existing enzyme in the mixture. Hunter & Yothangrong (2006)
  • 17. Formula 4: Dishwashing Liquid Enzyme (base) Base will consist 15 Kilograms heavily of citrus fruits Coconut oil or CDE 2 kilogram Ash water 15 kilograms
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  • 19. Sodium Ammonian MonoEA DEA TEA Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate Lauryl Sulphate Solubility Increases Viscosity Decreases Cold Storage Improves Mildness Increases Cost Increases Use as an ingredient in standard formulations
  • 20. Attributes Generics Discount Lower Middle Leader Pharmac y Solids 5-15% 5-25% 10-30% 10-35% 25-40% 25-40% Level Primary SLS SLS/ALS ALS/ ALS/ DEA LS DEA LS Surfactant SLES SLES MEA LS MEA LS TEA LS TEA LS Secondary Alkanolamides Alkanolamides Alkanolamides Alkyl (amido) Alkyl (amido) Alkyl (amido) Surfactant Alkyl (amido) Betaines & Betaines & Betaines & Betaines & Amine Amine Oxides Amine Oxides Amine Oxides Oxides Pacifier/Visc. Sodium Chloride Sodium Chloride Sodium Chloride Glycol Glycol Glycol Stearates Stearates Stearates Control Ethylene Glycols Ethylene Glycols Ethylene Glycols Functional Maybe in ascetic Maybe in ascetic Maybe in ascetic Number of Number of Number of Ingredients quantities quantities quantities choices choices choices Enzymes here Fragrance Low cost selections Low cost selections Low cost selections Higher cost Higher cost Higher cost selections selections selections Preservatives Formalin Formalin Sodium Benzoate Number of Number of Number of choices choices choices
  • 21. Shampoo System profile (what everything does) Function Potential Material Objective Primary surfactant ALS, ALES, TLS, SLES, NaDOBS Performance, strength, efficacy Secondary surfactant/ Coconut MEA, Cocamide MEA, Cocamidopropyl Tactile and sensory perception, impact, theme Foam Stabiliser Betaine or amine ingredients, efficacy Pacifier/Pearliser Ethylene Glycol Distearate, Glycol Stearate Tactile and sensory perception, general impression, impact Viscosity Control Sodium chloride or gums Ingredients, general impression, strength Preservatives Potassium Sorbate, Diazolidonyl urea, Methyl and Ingredients, general impression Propyl Parabens Functional Additives Guar, Hydroxypropyl Trimmonium Chloride Tactile and sensory perception, general Panthenol, Dimethicone, impression, spiritual Hydrolysed Keratin Protein impression, impact, efficacy, Enzymes here intangible benefits, signal attributes Fragrance Primarily aroma chemicals that are synthesized General impression, odour profile, impact, efficacy, used in conventional products, or natural essential intangible benefits, signal oils that become part of the active ingredients attributes Ascetic Ingredients Aloe Vera, Green Tea Extract, Panthenol General impression, intangible benefits, tactile and sensory perception, impact, signal attributes Dyes Mainly synthetic, more naturals General impression, signal attributes
  • 22. Auxiliary cleaner Anti dandruff Protein stain remover (mouthwash) Teeth whitener Skin whitener??? Skin softener Grease & oil remover Dead skin remover (anti aging) Odour remover Antiseptic???