SCHOTT BOROFLOAT 33 glass is manufactured using the microfloat method, which produces flat glass sheets with a mirror-like surface quality. The flatness of glass sheets can be influenced by various parameters such as warp, bow, total thickness variation, thickness tolerance, and thickness deviation. For Nexterion Glass B slides, flatness is defined as the accumulated thickness deviation including warp, intra-slide thickness deviation, and inter-slide thickness tolerance, which is typically less than or equal to 25 μm combined. Measurement using interferometry shows that the typical flatness across a single 75.6 x 25 mm Nexterion Glass B slide is less than or equal to 5 μm. The surface microroughness of Glass B