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CHEMISTRY
PROJECT

On
Determination
of The
Contents Of
Cold Drinks
PROJECT
PREPARED BY

RAJKIRA
N
It gives me a great
ACKNOWLEDGEMENT

pleasure to express
my gratitude towards
our chemistry teacher
Mrs. Vrinda Joshi for
her guidance, support
and encouragement
throughout the
duration of the
project. Without her
motivation & help, the
successful completion
of this project would
not have been
possible.
PURPOSE
In recent days, soft drink brands
were put into various questions
regarding their purity. News
flashed that they contain harmful
pesticide, which arouse many
interest in knowing its contents
because I have been drinking
them for years. I wanted to
confirm that whether the charge
imposed on these brands are
true or not.

Another fact which inspired me
to do this project is That I am in
touch with qualitative analysis
whose knowledge with other
factors helped me to do so.
INDEX
SL.NO CONTENT

I

Introduction

II

Theory

III

Apparatus

IV

Chemicals Required

V

Detection of Ph

VI

Test for Carbon Dioxide

VII

Test for Glucose

VIII

Test for Phosphate

IX

Test for Alcohol

X

Test for Sucrose

XI

Result

XII

Conclusion

XIII

Bibliography
INTRODUCTION
The era of cold drinks began in 1952
but the industrialization in India marked
its beginning with launching of Limca
and Goldspot by parley group of
companies. Since, the beginning of cold
drinks was highly profitable and luring,
many multinational companies launched
their brands in India like Pepsi and
Coke.
Now days, it is observed in general that
majority of people viewed Sprite,
Miranda, and Limca to give feeling of
lightness, while Pepsi and Thumps Up
to activate pulse and brain.
THEORY
Cold drinks of different brands are composed of alcohol,
carbohydrates, carbon dioxide, phosphate ions etc.
These soft
drinks give feeling of warmth, lightness and have a
tangy taste
which is liked by everyone. Carbon dioxide is responsible
for the
formation of froth on shaking the bottle.
The carbon dioxide gas is dissolved in water to form
carbonic acid
which is also responsible for the tangy taste.
Carbohydrates are the
naturally occurring organic compounds and are major
source of
energy to our body. General formula of carbohydrates is
CX (H2O)Y.
On the basis of their molecule size carbohydrates are
classified as:Monosaccharide, Disaccharides and Polysaccharides.
Glucose is a
monosaccharide with formula C6H12O6 .It occurs in Free
State in
the ripen grapes in bones and also in many sweet fruits. It is
also
present in human blood to the extent of about 0.1%.
Sucrose is one
of the most useful disaccharides in our daily life. It is widely
distributed in nature in juices, seeds and also in flowers of
many
plants. The main source of sucrose is sugar cane juice which
contain 15-20 % sucrose and sugar beet which has about
10-17 %
sucrose. The molecular formula of sucrose is C12H22O11. It
is
produced by a mixture of glucose and fructose. It is nonreducing
in nature whereas glucose is reducing. Cold drinks are a bit
acidic
in nature and their acidity can be measured by finding their
pH
value. The pH values also depend upon the acidic contents
such as
citric acid and phosphoric acid.
APPARATUS
Test Tube
Test Tube Holder
Test Tube Stand
Stop Watch
Beaker
Burner
pH Paper
Tripod Stand
China Dish
Wire Gauge
Water Bath
CHEMICALS
REQUIRED
* Iodine Solution
* Potassium Iodine
* Sodium Hydroxide
* Fehling‟s A & B
Solution
* Lime Water
* Concentrated HNO3
* Benedict Solution
* Ammonium
Molybdate
DETECTION OF PH
EXPERIMENT
Small samples of cold drinks of different brands were
taken in a test tube and put on the pH paper. The
change in the color of pH paper was noticed and was
compared with the standard pH scale.

OBSERVATION
SL.NO

NAME OF THE
DRINK

COLOUR
CHANGE

pH VALUE

1

COCA COLA

PINK

2.5-3

2

SPRITE

RED

3

3

LIMCA

PINKISH

4

4

FANTA

ORANGE

3-4

INFERENCE
Soft drinks are generally acidic because of the presence of
citric acid and phosphoric acid. pH values of cold drink
of different brands are different due to the variation in
amount of acidic contents.
TEST FOR CARBON DIOXIDE
EXPERIMENT
As soon as the bottles were opened, one by one
the sample was passed through lime water. The
lime water turned milky.

OBSERVATON
SL.NO

NAME OF
THE
DRINK

TIME
TAKEN
(SEC.)

CONCLUSION

1

COCA
COLA

26.5

CO2 PRESENT

2

SPRITE

21

CO2 PRESENT

3

LIMCA

35

CO2 PRESENT

4

FANTA

36

CO2 PRESENT

INFERENCE
All the soft drinks contain dissolved carbon dioxide in
water. The carbon dioxide (CO2) dissolves in water to form
carbonic acid, which is responsible for its tangy taste.

CHEMICAL REACTION INVOLVED
Ca(OH) 2(s) + CO2 (g)

CaCO 3 (s) + H2O(s)
TEST FOR GLUCOSE
EXPERIMENT
Glucose is a reducing sugar acid. Its presence is
detected by the following test:1. BENEDICTS’S REAGENT TEST:Small samples of cold drinks of different brands
were taken in a test tube and a few drops of
Benedict‟s reagent were added. The test tube was
heated for few seconds. Formation of reddish color
confirmed the presence of glucose in cold drinks.

OBSERVATION
SL.NO

NAME OF THE
DRINK

OBSERVATO
IN

CONCLUSION

1

COCA COLA

REDDISH
COLOUR ppt

GLUCOSE
PRESENT

2

SPRITE

REDDISH
COLOUR ppt

GLUCOSE
PRESENT

3

LIMCA

REDDISH
COLOUR ppt

GLUCOSE
PRESENT

4

FANTA

REDDISH
COLOUR ppt

GLUCOSE
PRESENT

INFERENCE
All the samples gave positive test for glucose with
Benedict‟s reagent .Hence all the drinks contain
glucose.
2. FEHLING’S SOLUTION TEST
Small samples of cold drinks of different brands
were taken in a test tube and a few drops of
Fehling‟s A solution and Fehling‟s B solution was
added
in equal amount. The test tube was heated in a
water bath for 10 minutes. Appearance of brown
precipitate confirmed the presence of glucose in
cold drinks.
OBSERVATON
SL.NO

NAME OF THE
DRINK

OBSERVATO
IN

CONCLUSION

1

COCA COLA

REDDISH
BROWN ppt

GLUCOSE
PRESENT

2

SPRITE

REDDISH
BROWN ppt

GLUCOSE
PRESENT

3

LIMCA

REDDISH
BROWN ppt

GLUCOSE
PRESENT

4

FANTA

REDDISH
BROWN ppt

GLUCOSE
PRESENT

INFERENCE
All the samples gave positive test for glucose with
Fehling‟s (A & B) solutions. Hence all the cold
drinks contain glucose.
TEST FOR
EXPERIMENT
PHOSPHATE
Small samples of each brand of cold drinks were
taken in separate test tubes and Ammonium
Molybdate followed by concentrated Nitric Acid
(HNO3)
was added to it. The solution was heated.
Appearance of canary-yellow precipitate
confirmed the presence of phosphate ions in cold
OBSERVATON
drinks. NAME OF THE OBSERVATOIN
CONCLUSION
SL.NO
DRINK

1

COCA COLA

CANARY-YELLOW PHOSPHATE
ppt
PRESENT

2

SPRITE

CANARY-YELLOW PHOSPHATE
ppt
PRESENT

3

LIMCA

CANARY-YELLOW PHOSPHATE
ppt
PRESENT

4

FANTA

CANARY-YELLOW PHOSPHATE
ppt
PRESENT

INFERENCE
All the soft drinks samples gave positive test for
phosphate ions. Hence all the cold drinks contain
phosphate.
CHEMICAL REACTION INVOLVED

NaHPO4 + 12(NH4)2MoO4
+ 21HNO3 + 3H+

(NH4)3PO4.12MoO3
+ 21HN4NO3 +
12H2O
TEST FOR ALCOHOL
EXPERIMENT
Small samples of each brand of cold drinks were
taken in separate test tubes and Iodine followed by
Potassium Iodide and Sodium Hydroxide (NaOH)
solution was added to each test tube. Then the test
tubes were heated in hot water bath for 30
minutes. Appearance of yellow colored precipitate
confirmed the presence of alcohol in cold drinks.
OBSERVATON
SL.NO

NAME OF THE
DRINK

OBSERVATOIN

CONCLUSION

1

COCA COLA

YELLOW ppt

ALCOHOL
PRESENT

2

SPRITE

YELLOW ppt

ALCOHOL
PRESENT

3

LIMCA

YELLOW ppt

ALCOHOL
PRESENT

4

FANTA

YELLOW ppt

ALCOHOL
PRESENT

INFERENCE
All the cold drinks samples gave positive test for
alcohol. Hence all the cold drinks contain glucose.

CHEMICAL REACTION INVOLVED
CH3CH2OH + 4I2
+6NaOH

CHI3 + HCOONa
+ 5NaI + 5H2O
TEST FOR SUCROSE
EXPERIMENT
5 separate ml samples of each brand of cold drinks
were taken in china dishes and were heated very
strongly until changes occur. Black colored residue
left confirmed the presence of sucrose in cold
drinks.

OBSERVATON
SL.NO

NAME OF THE
DRINK

OBSERVATOIN

CONCLUSION

1

COCA COLA

BLACK
RESIDUE ppt

SUCROSE
PRESENT

2

SPRITE

BLACK
RESIDUE ppt

SUCROSE
PRESENT

3

LIMCA

BLACK
RESIDUE ppt

SUCROSE
PRESENT

4

FANTA

BLACK
RESIDUE ppt

SUCROSE
PRESENT

INFERENCE
All the brands of cold drinks contain sucrose. But
amount of sucrose varies in each brand of drink.
Fanta contains highest amount of sucrose.
RESULT
After conducting several tests, it was concluded that
the different brands of
cold drinks namely:
1. Coca Cola
2. Sprite
3. Limca
4. Fanta
All contains glucose, alcohol, sucrose, phosphate
and carbon dioxide. All cold drinks are acidic in
nature. On comparing the pH value of different
brands Coca Cola is the most acidic and Limca is
least acidic of all the four brands taken.

CARBON DIOXIDE
Among the four samples of cold drinks taken,
Sprite has the maximum amount of dissolved
carbon dioxide and Fanta has the minimum
amount of dissolved carbon dioxide.
CONCLUSION
DIS-ADVANTAGES OF COLD
DRINKS are little more harmful than sugar
1. Soft drinks
solution. As they contain sugar in large amount
which cause problems in diabetes patients.
2. Soft drinks can cause weight gain as they
interfere with the body‟s natural ability to suppress
hunger feeling.
3. Soft drinks have ability to dissolve the calcium so
they are also harmful for our bones.
4. Soft drinks contain “phosphoric acid” which has a
pH of 2.8. So they can dissolve a nail in about 4
days.
5. For transportation of soft drinks syrup the
commercial truck must use the hazardous matter
place cards reserved for highly conceive material.
6. Soft drinks have also ability to remove blood so
they are very harmful to our body.

USES OF COLD DRINKS

1. Cold drinks can be used as toilet cleaners.
2. They can remove rust spots from chrome car
humpers.
3. They clean corrosion from car battery terminals.
4. Soft drinks are used as an excellent „detergent‟
to remove grease from clothes.
5. They can loose a rusted bolt.
BIBLIOGRAPHY
LABORATORY MANUAL OF
CHEMISTRY

ENCARTA ENCYCLOPEDIA 2009

www.wikipedia.com
www.chemistry.org

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Chemisry project

  • 3. It gives me a great ACKNOWLEDGEMENT pleasure to express my gratitude towards our chemistry teacher Mrs. Vrinda Joshi for her guidance, support and encouragement throughout the duration of the project. Without her motivation & help, the successful completion of this project would not have been possible.
  • 4. PURPOSE In recent days, soft drink brands were put into various questions regarding their purity. News flashed that they contain harmful pesticide, which arouse many interest in knowing its contents because I have been drinking them for years. I wanted to confirm that whether the charge imposed on these brands are true or not. Another fact which inspired me to do this project is That I am in touch with qualitative analysis whose knowledge with other factors helped me to do so.
  • 5. INDEX SL.NO CONTENT I Introduction II Theory III Apparatus IV Chemicals Required V Detection of Ph VI Test for Carbon Dioxide VII Test for Glucose VIII Test for Phosphate IX Test for Alcohol X Test for Sucrose XI Result XII Conclusion XIII Bibliography
  • 6. INTRODUCTION The era of cold drinks began in 1952 but the industrialization in India marked its beginning with launching of Limca and Goldspot by parley group of companies. Since, the beginning of cold drinks was highly profitable and luring, many multinational companies launched their brands in India like Pepsi and Coke. Now days, it is observed in general that majority of people viewed Sprite, Miranda, and Limca to give feeling of lightness, while Pepsi and Thumps Up to activate pulse and brain.
  • 7. THEORY Cold drinks of different brands are composed of alcohol, carbohydrates, carbon dioxide, phosphate ions etc. These soft drinks give feeling of warmth, lightness and have a tangy taste which is liked by everyone. Carbon dioxide is responsible for the formation of froth on shaking the bottle. The carbon dioxide gas is dissolved in water to form carbonic acid which is also responsible for the tangy taste. Carbohydrates are the naturally occurring organic compounds and are major source of energy to our body. General formula of carbohydrates is CX (H2O)Y.
  • 8. On the basis of their molecule size carbohydrates are classified as:Monosaccharide, Disaccharides and Polysaccharides. Glucose is a monosaccharide with formula C6H12O6 .It occurs in Free State in the ripen grapes in bones and also in many sweet fruits. It is also present in human blood to the extent of about 0.1%. Sucrose is one of the most useful disaccharides in our daily life. It is widely distributed in nature in juices, seeds and also in flowers of many plants. The main source of sucrose is sugar cane juice which contain 15-20 % sucrose and sugar beet which has about 10-17 % sucrose. The molecular formula of sucrose is C12H22O11. It is produced by a mixture of glucose and fructose. It is nonreducing in nature whereas glucose is reducing. Cold drinks are a bit acidic in nature and their acidity can be measured by finding their pH value. The pH values also depend upon the acidic contents such as citric acid and phosphoric acid.
  • 9. APPARATUS Test Tube Test Tube Holder Test Tube Stand Stop Watch Beaker Burner pH Paper Tripod Stand China Dish Wire Gauge Water Bath
  • 10. CHEMICALS REQUIRED * Iodine Solution * Potassium Iodine * Sodium Hydroxide * Fehling‟s A & B Solution * Lime Water * Concentrated HNO3 * Benedict Solution * Ammonium Molybdate
  • 11. DETECTION OF PH EXPERIMENT Small samples of cold drinks of different brands were taken in a test tube and put on the pH paper. The change in the color of pH paper was noticed and was compared with the standard pH scale. OBSERVATION SL.NO NAME OF THE DRINK COLOUR CHANGE pH VALUE 1 COCA COLA PINK 2.5-3 2 SPRITE RED 3 3 LIMCA PINKISH 4 4 FANTA ORANGE 3-4 INFERENCE Soft drinks are generally acidic because of the presence of citric acid and phosphoric acid. pH values of cold drink of different brands are different due to the variation in amount of acidic contents.
  • 12. TEST FOR CARBON DIOXIDE EXPERIMENT As soon as the bottles were opened, one by one the sample was passed through lime water. The lime water turned milky. OBSERVATON SL.NO NAME OF THE DRINK TIME TAKEN (SEC.) CONCLUSION 1 COCA COLA 26.5 CO2 PRESENT 2 SPRITE 21 CO2 PRESENT 3 LIMCA 35 CO2 PRESENT 4 FANTA 36 CO2 PRESENT INFERENCE All the soft drinks contain dissolved carbon dioxide in water. The carbon dioxide (CO2) dissolves in water to form carbonic acid, which is responsible for its tangy taste. CHEMICAL REACTION INVOLVED Ca(OH) 2(s) + CO2 (g) CaCO 3 (s) + H2O(s)
  • 13. TEST FOR GLUCOSE EXPERIMENT Glucose is a reducing sugar acid. Its presence is detected by the following test:1. BENEDICTS’S REAGENT TEST:Small samples of cold drinks of different brands were taken in a test tube and a few drops of Benedict‟s reagent were added. The test tube was heated for few seconds. Formation of reddish color confirmed the presence of glucose in cold drinks. OBSERVATION SL.NO NAME OF THE DRINK OBSERVATO IN CONCLUSION 1 COCA COLA REDDISH COLOUR ppt GLUCOSE PRESENT 2 SPRITE REDDISH COLOUR ppt GLUCOSE PRESENT 3 LIMCA REDDISH COLOUR ppt GLUCOSE PRESENT 4 FANTA REDDISH COLOUR ppt GLUCOSE PRESENT INFERENCE All the samples gave positive test for glucose with Benedict‟s reagent .Hence all the drinks contain glucose.
  • 14. 2. FEHLING’S SOLUTION TEST Small samples of cold drinks of different brands were taken in a test tube and a few drops of Fehling‟s A solution and Fehling‟s B solution was added in equal amount. The test tube was heated in a water bath for 10 minutes. Appearance of brown precipitate confirmed the presence of glucose in cold drinks. OBSERVATON SL.NO NAME OF THE DRINK OBSERVATO IN CONCLUSION 1 COCA COLA REDDISH BROWN ppt GLUCOSE PRESENT 2 SPRITE REDDISH BROWN ppt GLUCOSE PRESENT 3 LIMCA REDDISH BROWN ppt GLUCOSE PRESENT 4 FANTA REDDISH BROWN ppt GLUCOSE PRESENT INFERENCE All the samples gave positive test for glucose with Fehling‟s (A & B) solutions. Hence all the cold drinks contain glucose.
  • 15. TEST FOR EXPERIMENT PHOSPHATE Small samples of each brand of cold drinks were taken in separate test tubes and Ammonium Molybdate followed by concentrated Nitric Acid (HNO3) was added to it. The solution was heated. Appearance of canary-yellow precipitate confirmed the presence of phosphate ions in cold OBSERVATON drinks. NAME OF THE OBSERVATOIN CONCLUSION SL.NO DRINK 1 COCA COLA CANARY-YELLOW PHOSPHATE ppt PRESENT 2 SPRITE CANARY-YELLOW PHOSPHATE ppt PRESENT 3 LIMCA CANARY-YELLOW PHOSPHATE ppt PRESENT 4 FANTA CANARY-YELLOW PHOSPHATE ppt PRESENT INFERENCE All the soft drinks samples gave positive test for phosphate ions. Hence all the cold drinks contain phosphate. CHEMICAL REACTION INVOLVED NaHPO4 + 12(NH4)2MoO4 + 21HNO3 + 3H+ (NH4)3PO4.12MoO3 + 21HN4NO3 + 12H2O
  • 16. TEST FOR ALCOHOL EXPERIMENT Small samples of each brand of cold drinks were taken in separate test tubes and Iodine followed by Potassium Iodide and Sodium Hydroxide (NaOH) solution was added to each test tube. Then the test tubes were heated in hot water bath for 30 minutes. Appearance of yellow colored precipitate confirmed the presence of alcohol in cold drinks. OBSERVATON SL.NO NAME OF THE DRINK OBSERVATOIN CONCLUSION 1 COCA COLA YELLOW ppt ALCOHOL PRESENT 2 SPRITE YELLOW ppt ALCOHOL PRESENT 3 LIMCA YELLOW ppt ALCOHOL PRESENT 4 FANTA YELLOW ppt ALCOHOL PRESENT INFERENCE All the cold drinks samples gave positive test for alcohol. Hence all the cold drinks contain glucose. CHEMICAL REACTION INVOLVED CH3CH2OH + 4I2 +6NaOH CHI3 + HCOONa + 5NaI + 5H2O
  • 17. TEST FOR SUCROSE EXPERIMENT 5 separate ml samples of each brand of cold drinks were taken in china dishes and were heated very strongly until changes occur. Black colored residue left confirmed the presence of sucrose in cold drinks. OBSERVATON SL.NO NAME OF THE DRINK OBSERVATOIN CONCLUSION 1 COCA COLA BLACK RESIDUE ppt SUCROSE PRESENT 2 SPRITE BLACK RESIDUE ppt SUCROSE PRESENT 3 LIMCA BLACK RESIDUE ppt SUCROSE PRESENT 4 FANTA BLACK RESIDUE ppt SUCROSE PRESENT INFERENCE All the brands of cold drinks contain sucrose. But amount of sucrose varies in each brand of drink. Fanta contains highest amount of sucrose.
  • 18. RESULT After conducting several tests, it was concluded that the different brands of cold drinks namely: 1. Coca Cola 2. Sprite 3. Limca 4. Fanta All contains glucose, alcohol, sucrose, phosphate and carbon dioxide. All cold drinks are acidic in nature. On comparing the pH value of different brands Coca Cola is the most acidic and Limca is least acidic of all the four brands taken. CARBON DIOXIDE Among the four samples of cold drinks taken, Sprite has the maximum amount of dissolved carbon dioxide and Fanta has the minimum amount of dissolved carbon dioxide.
  • 19. CONCLUSION DIS-ADVANTAGES OF COLD DRINKS are little more harmful than sugar 1. Soft drinks solution. As they contain sugar in large amount which cause problems in diabetes patients. 2. Soft drinks can cause weight gain as they interfere with the body‟s natural ability to suppress hunger feeling. 3. Soft drinks have ability to dissolve the calcium so they are also harmful for our bones. 4. Soft drinks contain “phosphoric acid” which has a pH of 2.8. So they can dissolve a nail in about 4 days. 5. For transportation of soft drinks syrup the commercial truck must use the hazardous matter place cards reserved for highly conceive material. 6. Soft drinks have also ability to remove blood so they are very harmful to our body. USES OF COLD DRINKS 1. Cold drinks can be used as toilet cleaners. 2. They can remove rust spots from chrome car humpers. 3. They clean corrosion from car battery terminals. 4. Soft drinks are used as an excellent „detergent‟ to remove grease from clothes. 5. They can loose a rusted bolt.
  • 20. BIBLIOGRAPHY LABORATORY MANUAL OF CHEMISTRY ENCARTA ENCYCLOPEDIA 2009 www.wikipedia.com www.chemistry.org