The document describes an experiment to extract DNA from strawberries using different alcohol temperatures. Students predicted DNA would extract most efficiently from colder alcohol. They crushed strawberries mixed with salt water and filtered it. DNA was extracted by adding alcohol and forming white clumps, which was observed for all alcohol temperatures tested. The conclusion was the hypothesis was incorrect as DNA could be extracted from all temperatures.

1. Extracting DNA from Strawberries Tania Ramos Rossini Batino Vivian Pham Anthony Mendoza

2. PURPOSE To see if we can extract DNA from strawberries at different temperature levels.

3. HYPOTHESIS We predicted that at lower alcohol temperature the DNA from the strawberries will be extracted most efficiently.

4. Materials Ziplocs coffee filters Toothpicks Camera Laptop

5. Procedures Step 1.3 beakers with 3 diff. alcohol temp. Put strawberries in the bag Crush strawberries Step 2 mix salt, tap water and soap . And add it to the crushed strawberries Step 3 filter with coffee filter Step 4 Take the filter off and add the ice cold alcohol to the mixture in the glass. Step 5 After about 5 minutes, you should see a clumpy white substance beginning to form. This is the DNA. The alcohol allows the DNA to float. Use the toothpick or the craft stick to pick up the DNA so you can look at its strands more closely.

6. Procedure Step 1 + Step 2 Cold Regular Hot Step 3

7. REGULAR HOT COLD

8. Data

9. Conclusions We concluded that our hypothesis was incorrect: DNA CANbe extracted from all alcohol temperatures.

10. Work Cited Cziurczack, Ellen. "How to Extract DNA from strawberries". ehow. June 3,2010 <http://www.ehow.com/how_5031799_extract-dna-strawberry.html>. "Alcohol boiling points". ToolBox. June 3,2010 <http://www.engineeringtoolbox.com/boiling-points-fluids-gases-d_155.html>. "Dangers to boiling rubbing alcohol". The Straight Dope. June 1, 2010 <http://boards.straightdope.com/sdmb/showthread.php?t=284931>. This project is from the "Understanding Genetics: Human Health and the Genome" exhibit at The Tech Museum of Innovation in San Jose, CA: The Tech, 2004. "Making Medicine: Do-It-Yourself Strawberry DNA," The Tech Museum of Innovation, San Jose, CA. [accessed March 6, 2007]http://www.thetech.org/genetics/medicine.php Another similar project can be found from the Santa Clara College Biotechnology Education Program (SCCBEP) at San Jose State University: SCCBEP, 2002. "DNA Spooling from Strawberries," Santa Clara College Biotechnology Education Program (SCCBEP), San Jose State University, CA. [accessed March 6, 2007] http://www.science.sjsu.edu/sccbep/resources/curriculum/dna_spooling_strawberry.pdf Visit the online "Understanding Genetics: Human Health and the Genome" exhibit at the Tech Museum of Innovation and click on the "Zooming Into DNA" online exhibit. This is a great way to understand and visualize where the DNA in your body is: Rosa, C. et. al., 2007. "Zooming Into DNA," The Tech Museum of Innovation, San Jose, CA. [accessed March 6, 2007]http://www.thetech.org/genetics/index.php Want to try a DNA Extraction the way it is done in a real laboratory? Try the DNA Extraction Virtual Laboratory from the University of Utah: Univ. of Utah, 2006. "DNA Extraction Virtual Lab," The Genetic Science Learning Center, University of Utah, Salt Lake City, UT. [accessed March 6, 2007] http://learn.genetics.utah.edu/units/biotech/extraction/