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Chapter 2: Cells

                     Animal cell                                               Plant cell




*2, 11 and 14 not in syllabus.                          *5 not in syllabus
S/N      Organelle          Form           Function     S/N       Organelle         Form           Function

                                         Allows cer-
                                         tain sub-
                                         stances in
                                         and out of
                                         the cell by
                                                                                   Made of
                           Partially     controlling
                                                                                 non-living     Provides me-
                         permeable       the size of
        Cell surface                                                              and fully     chanical sup-
 1                       biphospho-      the pores.      1        Cell wall
        membrane                                                                 permeable      port, gives cell
                         lipid mem-      Movement
                                                                                 rigid cellu-   its fixed shape
                            brane        of particles
                                                                                     lose
                                         based on
                                         size and
                                         concentra-
                                         tion of par-
                                         ticles.


                                                                                                Allows certain
                                         Stores and                                             substances in
                                         modifies                                               and out of the
                         Shaped like     substances                                             cell by con-
                                                                                   Partially    trolling the
                         a disc, stack   made by
                                                                                 permeable      size of the
       Golgi appara-     of flattened    ERs, pack-             Cell surface
 3                                                       2                       biphospho-     pores. Move-
            tus          spaces sur-     ages sub-              membrane
                                                                                 lipid mem-     ment of par-
                         rounded by      stances in
                                                                                    brane       ticles based on
                          membrane       vesicles to
                                         be trans-                                              size and con-
                                         ported out                                             centration of
                                         of cell.                                               particles.
Contains en-
                                                                               Gel-like
                                                                                            zymes and
                                                                             protoplasm
                                       Makes pro-                                           organelles,
                                                                               between
 4     Free ribosome         -         teins for the    3      Cytoplasm                    medium for
                                                                             nucleus and
                                       cell’s usage                                         cell’s bio-
                                                                             cell surface
                                                                                            chemical reac-
                                                                              membrane
                                                                                            tions.

                        Continuous
                        with nuclear   Ribosomes                             More tubu-
                          envelope,    on the RER                              lar than
                                                              Smooth en-
       Rough endop-      ribosomes     makes pro-                            RER, con-      Synthesis fats
                                                              doplasmic
 5     lasmic reticu-    attached to   teins usually    4                     nected to     and steroids,
                                                               reticulum
         lum (RER)        outer sur-   transported                            RER, no       detoxifies cell.
                                                                 (SER)
                        face of RER    out of the                            ribosomes
                           (causes     cell.                                  attached
                         roughness)


                          Contains                                             Contains
         Nucleus                       Contains                 Nucleus
                         chromatin,                                           chromatin,    Contains ge-
9–                                     genetic in-     9–
                        long thread-                                         long thread-   netic informa-
6, 7    (Nucleolus,                    formation       6, 7    (Nucleolus,
                         like struc-                                          like struc-   tion during
and    Nucleoplasm,                    during pro-     and    Nucleoplasm,
                         tures with                                           tures with    protein syn-
 8        Nuclear                      tein synthe-     8        Nuclear
                          DNA in                                               DNA in       thesis.
         envelope)                     sis.                     envelope)
                            them.                                                them.


                                                                             Continuous
                        More tubu-                                           with nuclear
                                                                                            Ribosomes on
                          lar than                                             envelope,
        Smooth en-                     Synthesis               Rough en-                    the RER
                        RER, con-                                             ribosomes
        doplasmic                      fats and ste-           doplasmic                    makes pro-
10                       nected to                     10                     attached to
         reticulum                     roids, detox-           reticulum                    teins usually
                         RER, no                                               outer sur-
           (SER)                       ifies cell.               (RER)                      transported
                        ribosomes                                            face of RER
                                                                                            out of the cell.
                         attached                                               (causes
                                                                              roughness)


                                       Contains
                                       enzymes
                          Gel-like
                                       and orga-
                        protoplasm
                                       nelles, me-                                          Makes pro-
                          between                              Free ribo-
12      Cytoplasm                      dium for        11                         -         teins for the
                        nucleus and                              some
                                       cell’s bio-                                          cell’s usage
                        cell surface
                                       chemical
                         membrane
                                       reactions.
Aerobic res-                             Oval struc-
                                       piration oc-                              tures with
                         Small sau-    curs here to                             membranes
       Mitochon-                                                                                Site for photo-
13                      sage-shaped    provide          12     Chloroplast      containing
         drion                                                                                  synthesis
                         organelle     energy for                               chlorophyll
                                       cellular ac-                               and fluid
                                       tivities.                                   stroma


                                                                                Large cen-      Contains dis-
                                       Essential for                           tral vacuole     solved sub-
                         Small hol-
                                       cell division                            containing      stances (eg.
-       Centriole       low cylind-                     13       Vacuole
                                       (mitosis/                               cell sap that    sugar, mineral
                            ers
                                       meiosis)                                 is enclosed     salts and ami-
                                                                               by tonoplast     no acids)


                                                                                                Aerobic respi-
                        Temporary
                                       Contain wa-                                              ration occurs
                        fluid-filled                                            Small sau-
                                       ter and food             Mitochon-                       here to pro-
-        Vacuole        spaces en-                      14                     sage-shaped
                                       substances                 drion                         vide energy
                        closed by a                                             organelle
                                       temporarily.                                             for cellular
                        membrane
                                                                                                activities.




                      Plant cell                                             Animal cell

Cell wall present since plants do not have a skeletal   Cell wall absent since animals have a skeletal sys-
system to provide support.                              tem to provide support.


Chloroplasts present to make food during photo-         Chloroplasts absent since animals can move about
synthesis since they can’t feed.                        and feed to provide energy for their activities.


Centrioles absent for cell division.                    Centrioles present for cell division.


A large central vacuole that is permanent.              Numerous, small and temporary vacuoles.
Cell structure                                       Adaptation

                                                  1. No nucleus means more space in the cell to
                                                     carry more haemoglobin and hence more oxy-
                                                     gen.
                 Red blood cell
                                                  2. Circular biconcave shape increases surface area
                                                     to volume ratio, increasing the rate of diffusion
                                                     of oxygen in and out of the cell.


                                                  1. Do not have cross walls or protoplasm enables
                                                     water to move through the lumen easily with
                                                     little need to diffuse.
                 Xylem vessel
                                                  2. Lignin is deposited on the walls to strengthen it
                                                     and also to prevent the vessels from collapsing.


                                                  1. Root hair cell has a long and narrow protrusion,
                                                     increasing the surface area to volume ration of
                 Root hair cell
                                                     the cell, increasing the rate of absorption of wa-
                                                     ter into the root hair cell.




                                                     Organ
   Cells     Form        Organs       Form                             Form          Organism
                                                    systems


Stomach cells + other cells  Stomach + other organs  Digestive system + other systems  Organism
Chapter 3: Movement of Substances (Diffusion, osmosis, active transport)

Type of movement               Diffusion                    Osmosis                  Active transport

                       Diffusion is the net         Osmosis is the net           Active transport is the
                       movement of particles        movement of particles        process whereby energy
                       from a region of higher      from a region of higher      is used to move the par-
                       concentration to a re-       water potential to a re-     ticles against a concen-
                       gion of lower concentra-     gion of lower water po-      tration gradient from a
    Definition
                       tion down a concentra-       tential through a partial-   region of lower concen-
                       tion gradient without the    ly permeable membrane        tration to a region of
                       use of energy, with or       without the use of ener-     higher concentration
                       without a partially per-     gy.
                       meable membrane.


                       Nutrients diffuse into       Due to turgor pressure       Active transport is
                       ameoba to provide food       (refer to below), non-       needed in the uptake of
                       for the ameoba and           woody plants are able to     glucose in the villi in
                       waste products diffuse       stand firm and erect.        the small intestine to
                       out of the ameoba to                                      increase the amount of
                       prevent accumulation of                                   glucose being absorbed
                       harmful waste products.      Certain flowers are able     because the amount ab-
                                                    to open in the day and       sorbed through diffusion
                                                    close at night as the        is too little and a lot of
                       Oxygen present in the        changes in turgidity of      glucose will go to waste
                       air we inhale dissolves      the cells on opposite        if only diffusion is used.
                       into the thin layer of       surfaces of the petals
                       moisture on the alveolar     causes the petals to
                       wall before diffusing        bend.                        Active transport is
                       through the lung cell                                     needed in the ion uptake
                       into the blood stream to                                  by root hairs. In order to
Importance to living
                       be transported to all        The leaflets of the Mi-      maintain the low water
       cells
                       parts of the body. Car-      mosa plant fold when         potential, ions have to
                       bon dioxide moves from       touched due to changes       be absorbed as much
                       the blood vessels            in turgor of cells that      hence relying on diffu-
                       through the lung cell to     make up small swellings      sion alone won’t be suf-
                       the alveolar wall and out    at the base of the leaf-     ficient.
                       via the respiratory sys-     lets.
                       tem.

                                                    Changes in the turgor of
                       Carbon dioxide diffuses      guard cells causes the
                       into the intercellular air   opening and closing of
                       spaces before dissolving     the stomata (refer to
                       into the moisture to dif-    chapter 7)
                       fuse to the palisade me-
                       sophyll to be used in
                       photosynthesis.
Plant cells                                Animal cells

                         The cell sap has lower potential that      The cytoplasm of the animal cell has a
                         that of the solution outside the living    lower water potential than that the out-
                         cell hence water enters the cell by        side of the living cell hence water en-
                         osmosis. This increases the vacuole’s      ters the cell by osmosis. This increases
                         size and causes the vacuole to push        the size of the cell.
                         the cell contents against the cell wall.
                                                                    Since it does not have a cell wall, the
 Placed in solution of   Since the cell wall is strong and rela-    cell wall will expand until it bursts due
higher water potential   tively inelastic, it opposes the pres-     to the high pressure against the cell
                         sure to prevent more water from            surface membrane.
                         coming in and also prevents over-
                         expansion of the cell.

                         As it expands, it becomes turgid but
                         it doesn’t burst due to the cell wall’s
                         protection.


                         The cell sap has higher water poten-       The cytoplasm of the animal cell has a
                         tial that that of the solution outside     higher water potential than that of the
                         the living cell hence water leaves the     solution outside the living cell hence
Placed in solution of    cell by osmosis. This decreases the        water leaves the cell by osmosis. This
lower water potential    vacuole’s size and causes the cytop-       decreases the cell’s size until eventual-
                         lasm shrinks away from the cell wall.      ly it crenates and little spikes appear on
                         This causes the cell to be plasmo-         the cell surface membrane.
                         lysed.
Chapter 4: Nutrients

Important substance
                                        Characteristics                  Importance to living organisms
   body needs

                      General formula: CnH2mOm                           1. Substrate for respiration to
                                                                            provide energy for cellular
                      Divided into three groups: Monosaccharides            activities
                      (glucose, fructose, galactose), Disaccharides
                                                                         2. Forms supporting structures
                      (maltose, lactose, sucrose), Polysaccharides
                                                                            (eg. cellulose cell wall)
                      (starch, cellulose, glycogen)
                                                                         3. Converted to amino acids
                      Glucose + Glucose ⇌ Maltose + Water                   and fats
                      Glucose + Fructose ⇌ Sucrose + Water
                      Glucose + Galactose ⇌ Lactose + Water              4. Needed for formation of
  Carbohydrates                                                             nucleic acids (eg. DNA)
                      *Reversible reactions catalysed by enzymes
                      *This way is condensation reaction, the other      5. Needed to synthesise lubri-
                      way hydrolytic reaction (occurs in digestive          cants (eg. mucus)
                      system)
                                                                         6. Needed to synthesise nectar
                      Excess sugars are stored are polysaccharides as
                      they are insoluble so they don’t change water
                      potential, are large molecules so cannot diffuse
                      out through cell membranes, are easily hydro-
                      lysed to glucose when needed and are in com-
                      pact shapes which saves space.


                      General formula: No fixed proportions, little      1. Source and store of energy
                      oxygen as compared to hydrogen. Made of C,
                      H and O.                                           2. Insulating material prevent-
                                                                            ing heat loss
                      Fat molecule + 3Water 
                      Glycerol + 3Fatty acid molecules                   3. Solvent for fat-soluble vi-
                                                                            tamins
                       Saturated fats           Unsatured fats
                       Found mostly in an-      Found mostly in          4. Essential part of protoplasm
                       imals                    vegetables                  (eg. biphospholipid cell
       Fats                                                                 membrane)
                       Fatty acid chains are    Fatty acid chains are    5. Reduces water loss by se-
                       straight                 bent in some places         creting an oily substance
                                                                            over skin, reducing rate of
                       Solid at rtp             Liquid at rtp               evaporation


                      Cholestrol are usually found with polysaturated
                      fats and this may cause coronary heart disease
                      or formation of gallstones.
General formula: Contains amino group (-          1. Needed in synthesis of new
           NH2), acidic group (-COOH) and a side chain          protoplasm, for growth and
           (R). R can contain sulfur, acidic groups, amino      repair of worn-out body
           groups and/or hydroxyl groups.                       cells

                                                             2. Needed in the synthesis of
                                                                enzymes and some hor-
                                                                mones
Proteins
                                                             3. Needed for the formation of
                                                                antibodies to combat dis-
                                                                eases



           *A peptide bond is formed between the two
           joined amino acids (polypeptides), one water
           molecules is formed.

                                                             1. Medium for biochemical
                                                                reactions in our body

                                                             2. Transports digested prod-
                                                                ucts, waste products and
                                                                hormones around the body

                                                             3. Key component of protop-
                                                                lasm, lubricants in joints,
                                                                digestive juices, blood and
                                                                tissue fluid

                                                             4. Needed for certain reactions
                                                                (eg. hydrolysis in digestion
 Water                            -
                                                                and photosynthesis)

                                                             5. Component of sweat, when
                                                                evaporated, removes latent
                                                                heat of vaporisation cooling
                                                                us down

                                                             6. Maintains turgor pressure in
                                                                plants to allow them to be
                                                                firm and erect

                                                             7. Needed to transport mineral
                                                                salts and food substances
                                                                from respective parts
Substance to be
                                      Test                      If present, …                If absent, …
     tested

                         Add 2 drops of potassium          Iodine will turn bluish-
      Starch                                                                            Iodine remains brown.
                         iodide to test sample.            black.


                         Add equal volumes of Bene-
                         dict solution (copper (II) sul-
                                                           Solution will turn from,
 Reducing sugars         fate) and test solution into a
                                                           with increasing concen-
(eg. glucose, mal-       test tube. Place the test tube                                 Solution remains light
                                                           tration of reducing sug-
tose, fructose, lac-     into a beaker of boiling water                                 blue.
                                                           ar, green to yellow to
        tose)            and remove within 5 mins or
                                                           orange to brick-red.
                         when the solution changes
                         colour.


                         Add half the volume of so-
                         dium hydroxide to the volume
                         of test sample. Add 1% cop-       Solution will turn vio-      Solution remains light
      Protein
                         per (II) sulfate solution, drop   let.                         blue.
                         by drop, shaking after every
                         drop.


                         Add ethanol to test solution      Cloudy white emulsion
                         and shake thoroughly. Add         will form in the solu-       Solution remains co-
       Fats
                         water to the mixture and          tion and sinks to the        lourless throughout.
                         shake.                            bottom.



                   Small molecules                                          Large molecules

                       Amino acids                                    Polypeptides form proteins


                        Glucose                                       Maltose, sucrose, galactose


                Glycerol and fatty acids                                             Fats
Chapter 5: Enzymes

        Characteristic                                           Explanation

                                 Enzymes speed up chemical reactions by lowering the activation
 Speed up chemical reactions
                                 energy needed to start the reaction.


                                 Since they remain unchanged after a reaction, they can be used over
Required in very small amounts   and over again. This means a small amount of enzyme is able to cata-
                                 lyse a large number of chemical reactions.


                                                                           Enzymes are highly specific
                                                                           due to its 3-dimensional
                                                                           shape. The lock and key hy-
                                                                           pothesis is able to explain
                                                                           why.


                                                                           According to the lock and key
                                                                           hypothesis, enzyme reaction
       Highly specific
                                                                           depends on the presence of
                                                                           active sites, depressions on
                                 the surface of an enzyme molecule into which the substrate mole-
                                 cule(s) can fit. When a substrate with a complementary shape to the
                                 shape of the active site binds to the enzyme, an enzyme-substrate
                                 complex is formed and reactions take place at the active sites to con-
                                 vert substrate molecule(s) to product molecule(s). The product mole-
                                 cule(s) separates, leaving the enzyme unchanged and free to recom-
                                 bine with more substrate molecule(s).

                                 Before optimum temperature: After optimum temperature:
                                 Raising the temperature in-       Enzymes are made of protein which
                                 creases the kinetic energy of     denatures under high temperatures.
                                 the substrates and the en-        High temperatures cause the atoms in
   Affected by temperature       zymes. This causes them to        the enzyme to vibrate very violent that
                                 collide more often, increas-      they break the hydrogen bonds in the
                                 ing the chance of substrates      enzyme causing it to lose its shape
                                 fitting into active sites. The    and active site (denaturation). With
                                 rate of formation of the en-      this the substrate cannot fit in the en-
                                 zyme-substrate complex in-        zyme anymore.
                                 creases leading to a higher
                                 rate of products formed.          *Denaturation is irreversible.
                                 Usually, per 10°C increase in
                                 temperature, the rate of reac-
                                 tion doubles.

                                 * Inactiveness is reversible.
Affected by pH            Changes in pH may cause the acidity or alkalinity to break the hydro-
                                gen bonds holding the coils in place. This extreme acidity or alkalinity
                                causes the enzymes to be denatured losing their ability to catalyse the
                                reactions.

                                *Denaturation is irreversible.




                                Enzymes catalyse reactions in the direction where there is a higher
Catalyse reversible reactions
                                concentration of reactants to the lower concentration of reactants.

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BIO - Chapter 2-5 - Cells, Movement of Substances, Nutrients and Enzymes

  • 1. Chapter 2: Cells Animal cell Plant cell *2, 11 and 14 not in syllabus. *5 not in syllabus S/N Organelle Form Function S/N Organelle Form Function Allows cer- tain sub- stances in and out of the cell by Made of Partially controlling non-living Provides me- permeable the size of Cell surface and fully chanical sup- 1 biphospho- the pores. 1 Cell wall membrane permeable port, gives cell lipid mem- Movement rigid cellu- its fixed shape brane of particles lose based on size and concentra- tion of par- ticles. Allows certain Stores and substances in modifies and out of the Shaped like substances cell by con- Partially trolling the a disc, stack made by permeable size of the Golgi appara- of flattened ERs, pack- Cell surface 3 2 biphospho- pores. Move- tus spaces sur- ages sub- membrane lipid mem- ment of par- rounded by stances in brane ticles based on membrane vesicles to be trans- size and con- ported out centration of of cell. particles.
  • 2. Contains en- Gel-like zymes and protoplasm Makes pro- organelles, between 4 Free ribosome - teins for the 3 Cytoplasm medium for nucleus and cell’s usage cell’s bio- cell surface chemical reac- membrane tions. Continuous with nuclear Ribosomes More tubu- envelope, on the RER lar than Smooth en- Rough endop- ribosomes makes pro- RER, con- Synthesis fats doplasmic 5 lasmic reticu- attached to teins usually 4 nected to and steroids, reticulum lum (RER) outer sur- transported RER, no detoxifies cell. (SER) face of RER out of the ribosomes (causes cell. attached roughness) Contains Contains Nucleus Contains Nucleus chromatin, chromatin, Contains ge- 9– genetic in- 9– long thread- long thread- netic informa- 6, 7 (Nucleolus, formation 6, 7 (Nucleolus, like struc- like struc- tion during and Nucleoplasm, during pro- and Nucleoplasm, tures with tures with protein syn- 8 Nuclear tein synthe- 8 Nuclear DNA in DNA in thesis. envelope) sis. envelope) them. them. Continuous More tubu- with nuclear Ribosomes on lar than envelope, Smooth en- Synthesis Rough en- the RER RER, con- ribosomes doplasmic fats and ste- doplasmic makes pro- 10 nected to 10 attached to reticulum roids, detox- reticulum teins usually RER, no outer sur- (SER) ifies cell. (RER) transported ribosomes face of RER out of the cell. attached (causes roughness) Contains enzymes Gel-like and orga- protoplasm nelles, me- Makes pro- between Free ribo- 12 Cytoplasm dium for 11 - teins for the nucleus and some cell’s bio- cell’s usage cell surface chemical membrane reactions.
  • 3. Aerobic res- Oval struc- piration oc- tures with Small sau- curs here to membranes Mitochon- Site for photo- 13 sage-shaped provide 12 Chloroplast containing drion synthesis organelle energy for chlorophyll cellular ac- and fluid tivities. stroma Large cen- Contains dis- Essential for tral vacuole solved sub- Small hol- cell division containing stances (eg. - Centriole low cylind- 13 Vacuole (mitosis/ cell sap that sugar, mineral ers meiosis) is enclosed salts and ami- by tonoplast no acids) Aerobic respi- Temporary Contain wa- ration occurs fluid-filled Small sau- ter and food Mitochon- here to pro- - Vacuole spaces en- 14 sage-shaped substances drion vide energy closed by a organelle temporarily. for cellular membrane activities. Plant cell Animal cell Cell wall present since plants do not have a skeletal Cell wall absent since animals have a skeletal sys- system to provide support. tem to provide support. Chloroplasts present to make food during photo- Chloroplasts absent since animals can move about synthesis since they can’t feed. and feed to provide energy for their activities. Centrioles absent for cell division. Centrioles present for cell division. A large central vacuole that is permanent. Numerous, small and temporary vacuoles.
  • 4. Cell structure Adaptation 1. No nucleus means more space in the cell to carry more haemoglobin and hence more oxy- gen. Red blood cell 2. Circular biconcave shape increases surface area to volume ratio, increasing the rate of diffusion of oxygen in and out of the cell. 1. Do not have cross walls or protoplasm enables water to move through the lumen easily with little need to diffuse. Xylem vessel 2. Lignin is deposited on the walls to strengthen it and also to prevent the vessels from collapsing. 1. Root hair cell has a long and narrow protrusion, increasing the surface area to volume ration of Root hair cell the cell, increasing the rate of absorption of wa- ter into the root hair cell. Organ Cells Form Organs Form Form Organism systems Stomach cells + other cells  Stomach + other organs  Digestive system + other systems  Organism
  • 5. Chapter 3: Movement of Substances (Diffusion, osmosis, active transport) Type of movement Diffusion Osmosis Active transport Diffusion is the net Osmosis is the net Active transport is the movement of particles movement of particles process whereby energy from a region of higher from a region of higher is used to move the par- concentration to a re- water potential to a re- ticles against a concen- gion of lower concentra- gion of lower water po- tration gradient from a Definition tion down a concentra- tential through a partial- region of lower concen- tion gradient without the ly permeable membrane tration to a region of use of energy, with or without the use of ener- higher concentration without a partially per- gy. meable membrane. Nutrients diffuse into Due to turgor pressure Active transport is ameoba to provide food (refer to below), non- needed in the uptake of for the ameoba and woody plants are able to glucose in the villi in waste products diffuse stand firm and erect. the small intestine to out of the ameoba to increase the amount of prevent accumulation of glucose being absorbed harmful waste products. Certain flowers are able because the amount ab- to open in the day and sorbed through diffusion close at night as the is too little and a lot of Oxygen present in the changes in turgidity of glucose will go to waste air we inhale dissolves the cells on opposite if only diffusion is used. into the thin layer of surfaces of the petals moisture on the alveolar causes the petals to wall before diffusing bend. Active transport is through the lung cell needed in the ion uptake into the blood stream to by root hairs. In order to Importance to living be transported to all The leaflets of the Mi- maintain the low water cells parts of the body. Car- mosa plant fold when potential, ions have to bon dioxide moves from touched due to changes be absorbed as much the blood vessels in turgor of cells that hence relying on diffu- through the lung cell to make up small swellings sion alone won’t be suf- the alveolar wall and out at the base of the leaf- ficient. via the respiratory sys- lets. tem. Changes in the turgor of Carbon dioxide diffuses guard cells causes the into the intercellular air opening and closing of spaces before dissolving the stomata (refer to into the moisture to dif- chapter 7) fuse to the palisade me- sophyll to be used in photosynthesis.
  • 6. Plant cells Animal cells The cell sap has lower potential that The cytoplasm of the animal cell has a that of the solution outside the living lower water potential than that the out- cell hence water enters the cell by side of the living cell hence water en- osmosis. This increases the vacuole’s ters the cell by osmosis. This increases size and causes the vacuole to push the size of the cell. the cell contents against the cell wall. Since it does not have a cell wall, the Placed in solution of Since the cell wall is strong and rela- cell wall will expand until it bursts due higher water potential tively inelastic, it opposes the pres- to the high pressure against the cell sure to prevent more water from surface membrane. coming in and also prevents over- expansion of the cell. As it expands, it becomes turgid but it doesn’t burst due to the cell wall’s protection. The cell sap has higher water poten- The cytoplasm of the animal cell has a tial that that of the solution outside higher water potential than that of the the living cell hence water leaves the solution outside the living cell hence Placed in solution of cell by osmosis. This decreases the water leaves the cell by osmosis. This lower water potential vacuole’s size and causes the cytop- decreases the cell’s size until eventual- lasm shrinks away from the cell wall. ly it crenates and little spikes appear on This causes the cell to be plasmo- the cell surface membrane. lysed.
  • 7. Chapter 4: Nutrients Important substance Characteristics Importance to living organisms body needs General formula: CnH2mOm 1. Substrate for respiration to provide energy for cellular Divided into three groups: Monosaccharides activities (glucose, fructose, galactose), Disaccharides 2. Forms supporting structures (maltose, lactose, sucrose), Polysaccharides (eg. cellulose cell wall) (starch, cellulose, glycogen) 3. Converted to amino acids Glucose + Glucose ⇌ Maltose + Water and fats Glucose + Fructose ⇌ Sucrose + Water Glucose + Galactose ⇌ Lactose + Water 4. Needed for formation of Carbohydrates nucleic acids (eg. DNA) *Reversible reactions catalysed by enzymes *This way is condensation reaction, the other 5. Needed to synthesise lubri- way hydrolytic reaction (occurs in digestive cants (eg. mucus) system) 6. Needed to synthesise nectar Excess sugars are stored are polysaccharides as they are insoluble so they don’t change water potential, are large molecules so cannot diffuse out through cell membranes, are easily hydro- lysed to glucose when needed and are in com- pact shapes which saves space. General formula: No fixed proportions, little 1. Source and store of energy oxygen as compared to hydrogen. Made of C, H and O. 2. Insulating material prevent- ing heat loss Fat molecule + 3Water  Glycerol + 3Fatty acid molecules 3. Solvent for fat-soluble vi- tamins Saturated fats Unsatured fats Found mostly in an- Found mostly in 4. Essential part of protoplasm imals vegetables (eg. biphospholipid cell Fats membrane) Fatty acid chains are Fatty acid chains are 5. Reduces water loss by se- straight bent in some places creting an oily substance over skin, reducing rate of Solid at rtp Liquid at rtp evaporation Cholestrol are usually found with polysaturated fats and this may cause coronary heart disease or formation of gallstones.
  • 8. General formula: Contains amino group (- 1. Needed in synthesis of new NH2), acidic group (-COOH) and a side chain protoplasm, for growth and (R). R can contain sulfur, acidic groups, amino repair of worn-out body groups and/or hydroxyl groups. cells 2. Needed in the synthesis of enzymes and some hor- mones Proteins 3. Needed for the formation of antibodies to combat dis- eases *A peptide bond is formed between the two joined amino acids (polypeptides), one water molecules is formed. 1. Medium for biochemical reactions in our body 2. Transports digested prod- ucts, waste products and hormones around the body 3. Key component of protop- lasm, lubricants in joints, digestive juices, blood and tissue fluid 4. Needed for certain reactions (eg. hydrolysis in digestion Water - and photosynthesis) 5. Component of sweat, when evaporated, removes latent heat of vaporisation cooling us down 6. Maintains turgor pressure in plants to allow them to be firm and erect 7. Needed to transport mineral salts and food substances from respective parts
  • 9. Substance to be Test If present, … If absent, … tested Add 2 drops of potassium Iodine will turn bluish- Starch Iodine remains brown. iodide to test sample. black. Add equal volumes of Bene- dict solution (copper (II) sul- Solution will turn from, Reducing sugars fate) and test solution into a with increasing concen- (eg. glucose, mal- test tube. Place the test tube Solution remains light tration of reducing sug- tose, fructose, lac- into a beaker of boiling water blue. ar, green to yellow to tose) and remove within 5 mins or orange to brick-red. when the solution changes colour. Add half the volume of so- dium hydroxide to the volume of test sample. Add 1% cop- Solution will turn vio- Solution remains light Protein per (II) sulfate solution, drop let. blue. by drop, shaking after every drop. Add ethanol to test solution Cloudy white emulsion and shake thoroughly. Add will form in the solu- Solution remains co- Fats water to the mixture and tion and sinks to the lourless throughout. shake. bottom. Small molecules Large molecules Amino acids Polypeptides form proteins Glucose Maltose, sucrose, galactose Glycerol and fatty acids Fats
  • 10. Chapter 5: Enzymes Characteristic Explanation Enzymes speed up chemical reactions by lowering the activation Speed up chemical reactions energy needed to start the reaction. Since they remain unchanged after a reaction, they can be used over Required in very small amounts and over again. This means a small amount of enzyme is able to cata- lyse a large number of chemical reactions. Enzymes are highly specific due to its 3-dimensional shape. The lock and key hy- pothesis is able to explain why. According to the lock and key hypothesis, enzyme reaction Highly specific depends on the presence of active sites, depressions on the surface of an enzyme molecule into which the substrate mole- cule(s) can fit. When a substrate with a complementary shape to the shape of the active site binds to the enzyme, an enzyme-substrate complex is formed and reactions take place at the active sites to con- vert substrate molecule(s) to product molecule(s). The product mole- cule(s) separates, leaving the enzyme unchanged and free to recom- bine with more substrate molecule(s). Before optimum temperature: After optimum temperature: Raising the temperature in- Enzymes are made of protein which creases the kinetic energy of denatures under high temperatures. the substrates and the en- High temperatures cause the atoms in Affected by temperature zymes. This causes them to the enzyme to vibrate very violent that collide more often, increas- they break the hydrogen bonds in the ing the chance of substrates enzyme causing it to lose its shape fitting into active sites. The and active site (denaturation). With rate of formation of the en- this the substrate cannot fit in the en- zyme-substrate complex in- zyme anymore. creases leading to a higher rate of products formed. *Denaturation is irreversible. Usually, per 10°C increase in temperature, the rate of reac- tion doubles. * Inactiveness is reversible.
  • 11. Affected by pH Changes in pH may cause the acidity or alkalinity to break the hydro- gen bonds holding the coils in place. This extreme acidity or alkalinity causes the enzymes to be denatured losing their ability to catalyse the reactions. *Denaturation is irreversible. Enzymes catalyse reactions in the direction where there is a higher Catalyse reversible reactions concentration of reactants to the lower concentration of reactants.