3. Trends and threats that water utilities
have to deal with
Top 10 Trends
•Climate change
•Urbanization
•Emerging technologies
•Globalisation
•Increased use of bottled water
•Consumer involvement
•Increase in energy costs
•Increased efficiency
•Emerging pollutants E-sessions.nl
•Infrastructure deterioriation
EU FP6 TECHNEAU; WssTP
3
19. Emerging technologies
Optics, ICT and life sciences
BioCOM (ANR): Rapid on-site E. coli detection system
- Fluorogenic substrate technique
- Very sensitive optical detection
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20. E.coli and coliforms
E. coli and coliforms,
7 hrs growth
30 min incubation
1: white = colocalization: E. coli
1 2: green = coliform
colony shape
2
coliform or E. coli?
2 mm
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21. Cryptosporidium
Automatic counting
Manual counting
Microscope: 105
Camera: 104
Automatic (software): 96
2 mm
ImageJ
Ecoli analysis plugin
Max size 10, Min circularity 0.75
Threshold Otsu, BG correct original
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22. Increased specificity
Combination of immunology and genetic methods
2 μm
Immunofluorescence Fluorescence in situ hybridisation IF + FISH
IF FISH
22
27. Innovation for healthy, sustainable and efficient
water
Health Sustainability
Efficiency Advance
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28. Acknowledgements
BTO Joint Research programme, Netherlands
• Program committee Microbiology
BIO-COM: ANR and related consortium
• Ilan Juran, Erich Shaw (W-Smart, France, USA)
• Jean Claude Robin (BIOCOM)
• Regis Colla (Lheritier, France)
• Philippe Jacques, Angélique Cocaign (ProBiogem, Uni Lille, France)
• Bruno Nguyen et al. (Eau de Paris, France)
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Notes de l'éditeur
10/12/11 Project is 1 year underway: results of the first year Trend analysis that will influence water sector: prioritised in top 10 (in diff EU regions (and SA) trends analysed with local stakeholders. Merged into top 10 of common trends (in coop with GWRC) Now: evaluate impact in different regions of the world (GWRC) Trend report Techneau/GWRC. Interested: leave card with me. Example WA4: database hazards, combination of different national databases, basis for WSP (Wsportal WHO)
(halve maan) example of odd colony shape (groene kolonie met klein wit puntje): coliform or E. coli ?