Comparison of H&E stain quality between the VENTANA HE 600 system and a traditional linear “dip and dunk” stainer

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Comparative Analysis
The VENTANA HE 600 system versus a traditional
linear stainer

2. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Study participant
Dr. Sandra Mueller, PETROGLYPH Pathology Services
• Comparison of H&E stain quality between the VENTANA HE 600
system and a traditional linear “dip and dunk” stainer (Leica Autostainer
XL)
• Dr. Mueller evaluated 480 slides total
– 240 slides per instrument - 40 slides per instrument per tissue sample
• 6 tissue samples: Lymphoma 1, Lymphoma 2, Melanoma, Gastric Ca, Lobular
Ca, Liver
– H&E stain quality assessed with four-point scale shown below with Dr.
Mueller’s definition for each rating:

3. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Results
The VENTANA HE 600 system improves H&E stain qu
3.8
Average quality
score out of 4.0
3.0
The VENTANA
HE 600 system
Average quality
score out of 4.0
Dip and dunk
stainer
Average H&E stain quality scores across all slides and tissue
samples

4. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Results
The VENTANA HE 600 system improves H&E stain qu
85%
rated excellent quality
26.2%
The VENTANA HE 600
system
Dip and dunk stainer
rated excellent quality
0%
rated poor quality
6.7%
rated poor quality

5. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Results
More slides rated excellent on the VENTANA HE 600
system
100%
more stains
rated excellent
77.5% 60%
Gastric Ca Lymphoma 1
more stains
rated excellent
more stains
rated excellent
Liver
45%
more stains
rated excellent
40% 30%
Lobular Ca Melanoma
more stains
rated excellent
more stains
rated excellent
Lymphoma 2
58.8%
more stains rated
excellent overall
“Why should pathologists refuse to settle for good enough? Because
patients deserve more than good enough! As physicians, we should strive
for excellence in patient care by asking how we can make it better.”
– Dr. Sandra Mueller, pathologist

6. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Impressions on H&E stain quality consistency
A sampling of quotes from Dr. Mueller
The VENTANA HE 600 system Dip and dunk stainer
• “This is very pretty tissue! Consistent.
Great detail. Nice H&E balance.”
• “Hematoxylin is very crisp, which
highlights all the nuclear features.”
• “VERY consistent staining. Very crisp
detail.”
• “This is a great stain. Beautiful
contrast! Very consistent.”
• “Tissue has an edge effect…eosin is less
differentiating…has a basophilic hue
compromising degree of contrast.”
• “Eosin is lacking…hematoxylin lacks
crispness.”
• “Eosin is inconsistent…hematoxylin has
pink hue in many nuclei.”
• “Good stain…not great contrast.”
“On the VENTANA HE 600 system, eosin was more differentiating, which
makes low power magnification more effective in achieving a diagnosis.”
– Dr. Sandra Mueller, pathologist

7. Confidential and proprietary to Ventana Medical Systems, Inc. For internal use only. Do not copy. Do not distribute.
Individual slide staining improves performance
Eliminates reagent carry over and other stain quality
defects
“Depletion, dilution, evaporation and oxidation –
all commonplace with dip and dunk methods –
are completely resolved with individual slide
staining on the VENTANA HE 600 system. Fewer
defects means fewer distractions, decreased eye
fatigue and improved diagnostic performance.”
– Dr. Sandra Mueller, pathologist

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