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Isolation and Transmission Electron
    Microscopic Characterization of
Bacteriophages in Sewage Specific for
E.coli O157:H7 and CitrobacterFreundii


     Salvatore Valenti-Microbiology
    Department of Biological Sciences
Salvatore Valenti
My Question?
• Can I prove that phages are present in the
  sample collected from a sewage plant by
  visualizing them via Transmission Electron
  Microscopy?

• By answering this question I will help future
  generations of Wagner microbiology students
  perform further analyses of bacteriophages.
Philips CM 100 Transmission Electron
            Microscope
E.Coli O157:H7 phage from my sample




               A   B




               C   D
Citrobacterphage from my sample




             A   B




             C   D
Conclusion
• Water samples from sewage plants are rich in
  phages forE. coli O157:H7 and
  Citrobacterfreundii as it was proven with
  electron microscopy.
• Having learned the techniques to isolate
  bacteriophages and visualize them with the
  electron microscope makes me more
  competitive in the job market.
Acknowledgements
•   Dr. Kathleen Bobbitt
•   Prof. Christopher Corbo
•   Zulmaire Franco
•   Dr. Roy Mosher
•   Dr. Adam Houlihan

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Salvatore Valenti

  • 1. Isolation and Transmission Electron Microscopic Characterization of Bacteriophages in Sewage Specific for E.coli O157:H7 and CitrobacterFreundii Salvatore Valenti-Microbiology Department of Biological Sciences
  • 3. My Question? • Can I prove that phages are present in the sample collected from a sewage plant by visualizing them via Transmission Electron Microscopy? • By answering this question I will help future generations of Wagner microbiology students perform further analyses of bacteriophages.
  • 4. Philips CM 100 Transmission Electron Microscope
  • 5. E.Coli O157:H7 phage from my sample A B C D
  • 6. Citrobacterphage from my sample A B C D
  • 7. Conclusion • Water samples from sewage plants are rich in phages forE. coli O157:H7 and Citrobacterfreundii as it was proven with electron microscopy. • Having learned the techniques to isolate bacteriophages and visualize them with the electron microscope makes me more competitive in the job market.
  • 8. Acknowledgements • Dr. Kathleen Bobbitt • Prof. Christopher Corbo • Zulmaire Franco • Dr. Roy Mosher • Dr. Adam Houlihan