Wendy Cladman CV and skills addendum

1. Wendy
CLADMAN
Scientific Research Professional
Experimental Design and Execution 519-268-0649
Method Development http://ca.linkedin.com/in/wendycladman
Laboratory Management wcladman@sympatico.ca
Career Summary
A skilled and accomplished laboratory professional with extensive experience conducting/analyzing
protocols and experiments. Expertise in laboratory management, equipment usage and maintenance,
instruction of personnel, writing reports and contributing to publications. A talent for analyzing and
resolving complex problems, developing and simplifying procedures. Works well as part of a team or
independently. Ability to adapt to different situations.
Critical Skill Set
 Biochemical assays  Data analysis  Cell culture
 Spectrophotometry  Laboratory management  Microbiology techniques
 Immunoprecipitations  Complex problem solving  Protein purification
 Liquid Chromatography  Computer skills  DNA and RNA extractions
 Molecular biology  Recombinant DNA protocols  Immunoblotting
 Preparation of reports  Gene expression  Radioactive assays
Professional Employment History
University of Western Ontario, Department of Biology, Dr. Stan Caveney
Laboratory Technician level 5 January 2005 – July 2008
Area of Research: Monoamine neurotransmitter transporters in invertebrates
 Conducted phylogenetic study of neurotransmitter transporters in invertebrates and
vertebrates using RT PCR, BLAST searches and genome banks, contributing to a
publication.
 Designed experiments to study the effects of kinases on the uptake and efflux functions of
the octopamine transporter in a live cell assay.
 Examined the functional role of neurotransmitter transporters in a moth by RNA
interference and quantitative PCR.
University of Western Ontario, Department of Physiology and Pharmacology, Dr. Peter Chidiac
Laboratory Technician level 5 October 1998 - November 2004
Area of Research: Regulation of G-proteins by receptors and RGS proteins
 Developed a new steady state GTPase assay and improved purification methods for RGS
proteins and G-proteins.
 Analyzed the function of several RGS proteins through a series of complex assays and
procedures.
 Set up and managed laboratory. Trained and supervised personnel and students in
laboratory protocols.
University of Guelph, Department of Food Science, Dr. Mansel Griffiths
Research Associate April 1997 - February 1998
Area of Research: Develop methods of detection of food pathogens, milk trials
 Collected, purified and classified bacteriophage specific for food pathogenic bacteria for
the detection of pathogens in food and food processing equipment.
 Performed milk trials to test and compare efficacy of polyethylene terephthalate (PET)
milk containers resulting in a publication.
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2. Wendy Cladman CV
University of Guelph, Department of Chemistry and Biochemistry, Dr. Alan Mellors
Research Assistant February 1991 - January 1997
Area of Research: Study of O-sialoglycoprotein endopeptidase and other proteins secreted by P.
haemolytica
 Developed better methods to purify enzymes secreted by bacteria causing shipping fever
in cattle.
 Designed an assay to accurately quantify the degree of substrate cleavage by the
glycoprotease of interest.
 Determined the efficacy of antibodies to be used in vaccine trials.
Contract Positions:
Diagnostics Biochem Canada, Dorchester, Ontario
Production of ELISA assay kits June 2012 - August 2012
University of Western Ontario, Department of Microbiology and Immunology, Dr. Miguel Valvano
Laboratory Technician level 5 February 2010 - June 2010
Area of Research: Lipopolysaccharide mechanism of assembly in infectious bacteria
University of Western Ontario, Department of Biology, Dr. John Wiebe
Laboratory Technician level 5 September 2008 – March 2009
Area of Research: Purification of progesterone metabolite receptor in breast cancer cells
Limagrain Canada Seeds Incorporated, Listowel, Ontario
Laboratory technician in a canola research station April 1998 – August 1998
Area of Research: Canola Research Station
Music Theater:
Pfalztheater Kaiserslautern and Vereinigten Städtische Bühnen Krefeld-Mönchengladbach,
Germany
Opera Singer September 1979 - August 1990
 Rehearsed and performed operas, operettas and musicals on stage, performed in
concerts.
Education and Training
Graduated with a Bachelor of Science Degree in the biological sciences from
York University, Toronto, Ontario, Canada
Additional Courses (audited and/or participated in)
University of Toronto
Medical Genetics 430H Recombinant DNA Methods – laboratory course
University of Guelph
Biochemistry 19-258 Introductory Biochemistry
Food Science 42-323 Food Microbiology – laboratory course
University of Western Ontario
Pharmacology 462 Advanced Principles of Pharmacology
Pharmacology 443 Pharmacology and Toxicology of Natural-Sourced Medicine
Pharmacology 355b Principles of Drug Action
Attended Great Lakes GPCR Retreat in 2000, 2002 and 2003
Other laboratory training:
 WHMIS Training, University of Western Ontario, Agriculture Canada
 Biohazard Safety, University of Western Ontario
 Radiation Safety Training, University of Western Ontario, Agriculture Canada
 UWO Financials Training - Electronic Purchase Requisitions and AP/PO Inquiries, University of
Western Ontario, 2010
 Laboratory and Environmental Waste Management Safety Workshop, University of Western
Ontario, 1998 and 2010
 Frequently attended seminars, equipment demonstrations, equipment training sessions and
trade shows.
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3. Wendy Cladman CV
Publications
Papers published in refereed journals
Donly, C.,Verellen, L., Cladman, W., Caveney, S. 2007. Functional comparison of full-length and N-
terminal-truncated octopamine transporters from Lepidoptera. Insect Biochemistry and
Molecular Biology, 37(9):933-940.
Caveney, S., Cladman, W., Verellen, L. and Donly, C. 2006. Ancestry of neuronal monoamine
transporters in the metazoan. The Journal of Experimental Biology 209: 4858-4868.
Hepler, J.R., Cladman, W., Ramineni, S., Hollinger, S. and Chidiac, P. 2005. A novel activity of RGS14
on Goα and Gi2α nucleotide binding and hydrolysis independent of its RGS domain and GDI
activity. Biochemistry, 44(14):5495-5502.
Bernstein, L.S., Ramineni, S., Hague, C., Cladman, W., Chidiac, P., Levey, A.I., and Hepler, J.R. 2004.
RGS2 binds directly and selectively to the M1 muscarinic acetylcholine receptor third intracellular
loop to modulate Gq/11α signaling. The Journal of Biological Chemistry 279:21248-21256.
Cladman, W.M. and Chidiac, P. 2002. Characterization and comparison of RGS2 and RGS4 as GTPase
activating proteins for M2 muscarinic receptor-stimulated Gi. Molecular Pharmacology
62:654-659.
Cladman, W.M., Scheffer, S., Goodrich, N. and Griffiths, M.W. 1998. Shelf life of milk packaged in
plastic containers with and without treatment to reduce light transmission. International Dairy
Journal. 8:629-636.
Cladman, W.M., Watt, M.-A.V., Dini, J.-P. and Mellors, A. 1996. The Pasteurella haemolytica
O-sialoglycoprotein endopeptidase is inhibited by zinc ions and does not cleave fetuin.
Biochemical and Biophysical Research Communications. 220:141-146.
Lee, C.W., Shewen, P.E., Cladman, W.M., Conlon, J.A.R., Mellors, A. and Lo, R.Y.C. 1994.
Sialoglycoprotease of Pasteurella haemolytica A1: Detection of antisialoglycoprotease antibodies
in sera of calves. Canadian Journal of Veterinary Research. 58: 93-98.
Published Abstracts
Caveney, S., Cladman, W.,Verellen, L., Donly, C. 2005. Origin and evolutionary history of metazoan
monoamine transporters. 15th Neuropharmacology Conference, New Perspectives in
Neurotransmitter Transporter Biology, Washington DC, USA, Abstract no. P68.
Desjardins, R.L., Cladman, W. and Chidiac, P. 2003. Evidence for stimulus trafficking at the M2
muscarinic receptor. Great Lakes GPCR Retreat, Honey Harbour, Ontario.
Zhao, Q., Cladman, W. and Chidiac, P. 2003. Receptor-dependent differences between RGS4 and
RGS5 as GTPase activating proteins for Go and Gi2. Great Lakes GPCR Retreat, Honey Harbour,
Ontario.
Roy, A.A., Cladman, W.M., Lemberg, K.E., Ferguson, S.S., Cook, M.A. and Chidiac, P. 2001. G protein
selectivity of RGS2 and RGS4. Society for Neuroscience 31st Annual Meeting, San Diego CA. USA,
Abstract no. 43.12.
Roy, A.A., Cladman, W.M., Lemberg, K.E. and Chidiac, P. 2001. Effects of Gi, Gq and Gs on the
intracellular localization of GFP-RGS2 and GPF-RGS4. Great Lakes GPCR Retreat, Bromont,
Quebec.
Cladman, W. and Chidiac, P. 2000. Stimulation of Gi GTPase activity by RGS2 and RGS4 in a
membrane-based assay system. Great Lakes GPCR Retreat, London, Ontario.
Mellors, A., Watt, M.-A., Shad, Y., Cladman, W.M., and Lo, R.Y.C. 1994. The O-
sialoglycoprotease endopeptidase of Pasteurella haemolytica. Canadian Federation of
Biological Science, Montreal.
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4. Wendy Cladman CV
Addendum
Laboratory Skills
Biochemical and enzymatic assays
 Assaying for proteins and enzymatic activity by UV-VIS spectrophotometric and fluorometric
procedures as well as by densitometry, autoradiography and radioactive incorporation
 Milk quality testing: protein, lipid and Vitamin A degradation, measurement of bacterial
contamination
 Vitamin and fatty acid extractions and quantitative analysis by HPLC and GC
 Immunoprecipitations, immunoblotting techniques (colorimetric and chemiluminescent), ELISA
assays, radioimmuno assays (RIA), electrophoresis, whole cell electroelution
 Steady state assays, G protein and/or receptor radioligand binding, densitometry, substrate
degradation assays, complex time course experiments, live cell uptake and efflux assays,
Microbiology and cell biology
 Maintaining mammalian and insect cell culture and baculovirus stocks, microscopy, cabbage
looper moth rearing
 Maintaining bacterial strains and isolating mutants, aerobic and anaerobic
 Making bacteriophage or virus lysates by infection or induction, purifying phage and phage DNA
(single and double stranded) by CsCl gradient ultracentrifugation
 Screening and classification of pathogenic bacteria by staining, catalase and oxidase tests,
biochemical screening media and API strips
Molecular biology
 Recombinant DNA techniques, RT PCR, quantitative PCR, RNA interference, RACE, gene
isolations, ligations, cloning, bioinformatics
 RNA extractions and purifications from invertebrate tissue samples, restriction enzyme
digestions, reverse transcriptions, DNA extractions, plasmid kits
 Transformation, induction and expression of cloned genes, denaturation and renaturation of
purified proteins
Gene expression and protein purification
 Purification and analysis of membrane-bound proteins, enzymes and glycoproteins by
precipitations; column chromatography with HPLC, HRLC, FPLC, low pressure, batch and gravity
systems utilizing ion exchange, affinity, adsorption and size exclusion resins; differential
centrifugation
 Preparation of crude extracts from bacterial cultures and supernatants, mammalian tissues,
mammalian and insect cell cultures by various methods (sonication, French Pressure cell, glass
bead homogenization, polytron, osmotic shock and enzymatic lysis)
Data analysis and computer skills
 Kinetic analysis and statistical programs (Anova, Origin, Prism, Sigma Plot)
 Sequence analysis: DNA Star, Oligo, Gene Bank, BLAST search
 Spread sheets and plots (Excel, Origin), word processing
 Quantification of purified proteins and immunoblot data, analysis of densitometric data
Laboratory management
 Maintenance and documentation of chemical stocks, lab supplies, data bases and resource
collections
 Maintenance of equipment and software; training of personnel in the use of equipment
 Assisting with the preparation of grant applications, reports and research publications
 Radioactive inventory and waste disposal
 Setup and organization of facilities, organization of meetings and schedules
Use and maintenance of laboratory equipment
 FPLC, HPLC, HRLC, GC, electroporator, Thermal cycler (PCR), Light cycler, Rotor Gene
 Liquid scintillation counter, Ultracentrifuge, High-speed centrifuge, electrophoresis
 Fluorchem imager, phospho-imager, spectrophotometer, fluorometer, lyophilizer
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