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Somé Koussao , Ouedraogo Jeremy, Belem Jerôme,Asante Isaac, Vernon Gracenand Danquah Eric
30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya
Sweetpotato production in Burkina Faso
412% increased of production from 27366 t in 2000 to 140,061t in 2011
mainly due to increase of production area
However, yield over time has been unstable.
Cultivars used are mainly white flesh = low nutritional potential
2
Fig1. Evolution of sweetpotato production and yield from 2000 to 2011. (DGSPA, 2012)
USAID, 2003
Nutritional status in the world
Burkina Faso
81% of children an 64% of their mother suffered fromVAD in rural BF
30/06 -04/07/2013
The objectives of this study were:
1) To develop high yield and beta-carotene rich sweetpotato
locally adapted and that meet farmers’ and consumers’
expectations
2) to estimate narrow sense heritability for economically and
nutritionally important traits
3) to estimate the genetic gain from selection of the breeding
product
APA 2013 - 9th triennal conference - Naivasha-Kenya
30/06 -04/07/2013
Population development using 2 sets:
Local cultivars of white to yellow flesh: used as female parents:
BF24, BF59, BF77, BF82 and BF92
Introduced OFSP (beta-carotene source) considered as male:
CIP199062.1, Resisto,Tainung.
Controlled crosses done at the INERA station of Kamboinsé
from November 2009 to February 2010
Standard crossing procedures for sweetpotato were applied
APA 2013 - 9th triennal conference - Naivasha-Kenya
30/06 -04/07/2013
Field evaluation
Established in three different agro-
ecological zones
130 F1 progenies and their 8 parents
involved (Total = 138)
In anAlpha Lattice design,The
experiment was done from July to
November 2010 and replicated in
2011
Fig 2 and 3. Evaluation sites and field
APA 2013 - 9th triennal conference - Naivasha-Kenya
30/06 -04/07/2013
DATA COLLECTION:
growth parameters:
vine length,
above ground fresh weight,
and storage root parameters:
Yield components (number/plant,
individual fresh weight and dry weight,
weight per plant)
Dry matter content
Beta-carotene content
DATA ANALYSES:
Narrow sense heritability (h2), was
estimated from parent-offspring using the
method described by Holland et al. (2003).
The genetic advance was calculated
according to Falconer and Mackay (1996)
Fig 4. Data collection in the field
APA 2013 - 9th triennal conference - Naivasha-Kenya
30/06 -04/07/2013
Family RtYield DM BetaCar
BF24xCIP 10.65±8.27 33.16±11.68 0.048±0.043
BF24xResisto 11.38±7.76 27.90±6.51 0.024±0.029
BF24xTainung 10.73±6.68 28.25±7.25 0.036±0.136
BF59xCIP 13.19±6.79 27.59±9.22 4.625±4.125
BF59xResisto 14.71±9.35 25.68±5.02 0.042±0.054
BF59xTainung 12.36±8.68 25.21±5.26 0.067±0.060
BF77xCIP 17.08±11.18 21.62±3.22 0.082±0.057
BF77xResisto 13.28±8.58 27.48±5.91 0.018±0.024
BF77xTainung 17.14±7.75 26.29±6.42 0.028±0.039
BF82xCIP 12.29±8.70 26.06±6.61 3.156±3.730
BF82xResisto 6.97±6.76 27.30±6.88 0.082±0.250
BF82xTainung 13.79±9.35 23.31±6.60 0.717±1.978
BF92xCIP 14.53±13.41 26.84±5.96 2.273±3.440
BF92xResisto 12.70±8.29 24.73±6.21 0.945±2.849
BF92xTainung 11.06±6.91 28.20±3.57 0.0467.060
Table 1. F1 family average performance for storage root yield, DM and β-carotene
APA 2013 - 9th triennal conference - Naivasha-Kenya
30/06 -04/07/2013
Generation Rtyield
(T/ha)
BetaCar
(mg/100g of
fresh root)
DM
(%)
Biomass
(kg/per
plot)
Rootnum
(Number
/plant)
IRtwgt
(g/plant)
VL
(m)
Offspring 12.83 0.89 26.22 15.05 2.96 134.23 1.70
Pfemale 8.26 0.28 29.61 7.15 2.58 97.15 1.43
Fmale 11.45 3.27 27.93 7.47 2.95 130.62 1.01
Mid-Parent 9.86 1.77 28.77 7.31 2.77 113.87 1.22
% increase/MP
%increase/Pf
30.12
55.33
-49.72
216.73
-8.86
-11.45
105.88
110.49
6.86
14.73
17.88
38.17
39.34
18.88
All entries *** ns *** *** *** * ***
Offspring *** *** *** *** *** *** ***
Offsp.vs(Pf+Pm) ** ns *** *** ns ns ***
Table 2. Comparison of parents and offspring mean performance and their significance
APA 2013 - 9th triennal conference - Naivasha-Kenya
*, **, *** Significance of F at the 5%, 1% and 0.1% levels, respectively.
30/06 -04/07/2013
Heritability estimate
Genetic advance (%)Farakoba Kouare Loumbila Combined
Storage root yield 0.20±0.25 0.58±0.25 0.58±0.32 0.21±0.16 5.85
Dry Matter 0.75±0.06 0.93±0.03 0.75±0.14 0.76±0.003 22.60
Beta-Carotene 0.43±0.63 0.49±0.44 0.97±0.02 0.90±0.039 3.37
Biomass yield 0.58±0.18 0 0.05±0.42 0.04±0.07 7.81
Root number per plot 0.62±0.44 0.52±0.38 0.57±0.27 0.41±0.21 8.03
Individual Root weight 0.69±0.14 0 0.55±0.34 0.27±0.12 6.35
Vine Length 0.33±0.42 0.57±0.28 0.16±0.07 0.48±0.28 0.26
Table 3. Narrow sense heritability (h2) estimate per location and genetic advance from improvement
APA 2013 - 9th triennal conference - Naivasha-Kenya
Genotypes Root yield
(t/ha)
Upgr
BiomYield
(t/ha)
Β-carotene
(mg/100g of
fresh weight)
Virus2 DM% Irwgt
BF82xTainung-8 20.33 16.33 0.48 2.33 23.2 179.15
BF82xTainung-20 19.67 17.23 0.78 3.33 21.82 242.68
BF82xCIP-17 18.56 14.78 3.92 1.17 28.45 118.18
BF80xTainung-2 18.11 11.89 2 2.33 19.27 203.89
BF82xTainung-24 17.83 25.89 8.29 1 21.79 136.43
BF92xCIP-6 17.11 17.83 6.44 1.83 26.61 175.9
BF59xCIP-4 16.78 21.56 8.32 1.83 24.81 116.15
BF24xTIB-3 16.17 17.28 7.66 2.67 27.33 116.72
BF59xTIB-6 15.22 11.39 4.36 2.33 21.48 275.94
BF82xCIP-18 15.22 30.11 2.32 1.5 22.81 186.07
BF59xCIP-1 13.56 18.33 8.32 1.17 27.09 110.82
BF82xTIB-4 13.5 10.33 1.03 2.17 30.06 145.36
The Best OFSP F1 genotypes that had significantly higher yield than the best check
(Caromex with 11.50 t/ha)
30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya
OFSP BF82xTainung.8 (20.33 t/ha)
WFSP BF59xTainung.5 (54.67 t/ha)
OFSP BF92xCIP.6 (17.11 t/ha)
30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya

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Increased sweetpotato production and beta-carotene content in Burkina Faso

  • 1. Somé Koussao , Ouedraogo Jeremy, Belem Jerôme,Asante Isaac, Vernon Gracenand Danquah Eric 30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 2. Sweetpotato production in Burkina Faso 412% increased of production from 27366 t in 2000 to 140,061t in 2011 mainly due to increase of production area However, yield over time has been unstable. Cultivars used are mainly white flesh = low nutritional potential 2 Fig1. Evolution of sweetpotato production and yield from 2000 to 2011. (DGSPA, 2012)
  • 3. USAID, 2003 Nutritional status in the world Burkina Faso 81% of children an 64% of their mother suffered fromVAD in rural BF
  • 4. 30/06 -04/07/2013 The objectives of this study were: 1) To develop high yield and beta-carotene rich sweetpotato locally adapted and that meet farmers’ and consumers’ expectations 2) to estimate narrow sense heritability for economically and nutritionally important traits 3) to estimate the genetic gain from selection of the breeding product APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 5. 30/06 -04/07/2013 Population development using 2 sets: Local cultivars of white to yellow flesh: used as female parents: BF24, BF59, BF77, BF82 and BF92 Introduced OFSP (beta-carotene source) considered as male: CIP199062.1, Resisto,Tainung. Controlled crosses done at the INERA station of Kamboinsé from November 2009 to February 2010 Standard crossing procedures for sweetpotato were applied APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 6. 30/06 -04/07/2013 Field evaluation Established in three different agro- ecological zones 130 F1 progenies and their 8 parents involved (Total = 138) In anAlpha Lattice design,The experiment was done from July to November 2010 and replicated in 2011 Fig 2 and 3. Evaluation sites and field APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 7. 30/06 -04/07/2013 DATA COLLECTION: growth parameters: vine length, above ground fresh weight, and storage root parameters: Yield components (number/plant, individual fresh weight and dry weight, weight per plant) Dry matter content Beta-carotene content DATA ANALYSES: Narrow sense heritability (h2), was estimated from parent-offspring using the method described by Holland et al. (2003). The genetic advance was calculated according to Falconer and Mackay (1996) Fig 4. Data collection in the field APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 8. 30/06 -04/07/2013 Family RtYield DM BetaCar BF24xCIP 10.65±8.27 33.16±11.68 0.048±0.043 BF24xResisto 11.38±7.76 27.90±6.51 0.024±0.029 BF24xTainung 10.73±6.68 28.25±7.25 0.036±0.136 BF59xCIP 13.19±6.79 27.59±9.22 4.625±4.125 BF59xResisto 14.71±9.35 25.68±5.02 0.042±0.054 BF59xTainung 12.36±8.68 25.21±5.26 0.067±0.060 BF77xCIP 17.08±11.18 21.62±3.22 0.082±0.057 BF77xResisto 13.28±8.58 27.48±5.91 0.018±0.024 BF77xTainung 17.14±7.75 26.29±6.42 0.028±0.039 BF82xCIP 12.29±8.70 26.06±6.61 3.156±3.730 BF82xResisto 6.97±6.76 27.30±6.88 0.082±0.250 BF82xTainung 13.79±9.35 23.31±6.60 0.717±1.978 BF92xCIP 14.53±13.41 26.84±5.96 2.273±3.440 BF92xResisto 12.70±8.29 24.73±6.21 0.945±2.849 BF92xTainung 11.06±6.91 28.20±3.57 0.0467.060 Table 1. F1 family average performance for storage root yield, DM and β-carotene APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 9. 30/06 -04/07/2013 Generation Rtyield (T/ha) BetaCar (mg/100g of fresh root) DM (%) Biomass (kg/per plot) Rootnum (Number /plant) IRtwgt (g/plant) VL (m) Offspring 12.83 0.89 26.22 15.05 2.96 134.23 1.70 Pfemale 8.26 0.28 29.61 7.15 2.58 97.15 1.43 Fmale 11.45 3.27 27.93 7.47 2.95 130.62 1.01 Mid-Parent 9.86 1.77 28.77 7.31 2.77 113.87 1.22 % increase/MP %increase/Pf 30.12 55.33 -49.72 216.73 -8.86 -11.45 105.88 110.49 6.86 14.73 17.88 38.17 39.34 18.88 All entries *** ns *** *** *** * *** Offspring *** *** *** *** *** *** *** Offsp.vs(Pf+Pm) ** ns *** *** ns ns *** Table 2. Comparison of parents and offspring mean performance and their significance APA 2013 - 9th triennal conference - Naivasha-Kenya *, **, *** Significance of F at the 5%, 1% and 0.1% levels, respectively.
  • 10. 30/06 -04/07/2013 Heritability estimate Genetic advance (%)Farakoba Kouare Loumbila Combined Storage root yield 0.20±0.25 0.58±0.25 0.58±0.32 0.21±0.16 5.85 Dry Matter 0.75±0.06 0.93±0.03 0.75±0.14 0.76±0.003 22.60 Beta-Carotene 0.43±0.63 0.49±0.44 0.97±0.02 0.90±0.039 3.37 Biomass yield 0.58±0.18 0 0.05±0.42 0.04±0.07 7.81 Root number per plot 0.62±0.44 0.52±0.38 0.57±0.27 0.41±0.21 8.03 Individual Root weight 0.69±0.14 0 0.55±0.34 0.27±0.12 6.35 Vine Length 0.33±0.42 0.57±0.28 0.16±0.07 0.48±0.28 0.26 Table 3. Narrow sense heritability (h2) estimate per location and genetic advance from improvement APA 2013 - 9th triennal conference - Naivasha-Kenya
  • 11. Genotypes Root yield (t/ha) Upgr BiomYield (t/ha) Β-carotene (mg/100g of fresh weight) Virus2 DM% Irwgt BF82xTainung-8 20.33 16.33 0.48 2.33 23.2 179.15 BF82xTainung-20 19.67 17.23 0.78 3.33 21.82 242.68 BF82xCIP-17 18.56 14.78 3.92 1.17 28.45 118.18 BF80xTainung-2 18.11 11.89 2 2.33 19.27 203.89 BF82xTainung-24 17.83 25.89 8.29 1 21.79 136.43 BF92xCIP-6 17.11 17.83 6.44 1.83 26.61 175.9 BF59xCIP-4 16.78 21.56 8.32 1.83 24.81 116.15 BF24xTIB-3 16.17 17.28 7.66 2.67 27.33 116.72 BF59xTIB-6 15.22 11.39 4.36 2.33 21.48 275.94 BF82xCIP-18 15.22 30.11 2.32 1.5 22.81 186.07 BF59xCIP-1 13.56 18.33 8.32 1.17 27.09 110.82 BF82xTIB-4 13.5 10.33 1.03 2.17 30.06 145.36 The Best OFSP F1 genotypes that had significantly higher yield than the best check (Caromex with 11.50 t/ha)
  • 12. 30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya OFSP BF82xTainung.8 (20.33 t/ha) WFSP BF59xTainung.5 (54.67 t/ha) OFSP BF92xCIP.6 (17.11 t/ha)
  • 13. 30/06 -04/07/2013APA 2013 - 9th triennal conference - Naivasha-Kenya