The Enzyme Linked Immunospot (ELISPOT) technique was developed by Cecil Czerkinskdy in 1983. ELISPOT is used for the detection of secreted proteins, such as cytokines and growth factors. ELISPOT is primarily used in immunology research in the following areas:

1. ELISPOT Vs ELISA
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2. ELISPOT
• The Enzyme Linked Immunospot
(ELISPOT) technique was developed by Cecil
Czerkinskdy in 1983. ELISPOT is used for the
detection of secreted proteins, such as
cytokines and growth factors

3. ELISPOT – Research Areas
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Transplantation – prediction of infectious risk
Vaccine development (IFNγ)
Th1/Th2, T-cell regulation analysis
Monocyte and Dendritic cell analysis
Autoimmune disease
Cancer – tumor antigens
Allergy
Viral infection monitoring and treatment

4. ELISPOT – Developed Well

5. Advantages of ELISPOT
• Sensitive assay
• Functional assay
• Adaptable

6. ELISA Vs ELISPOT
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ELISPOT will yield information about number of cells secreting cytokine of interest
(e.g. n/1 million cells)
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ELISA will yield information on the Concentration of the cytokine of interest
produced by all cells in the culture (e.g. pg/1 ml of supernatant)
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ELISA gives you information about how much cytokine is produced and secreted at a
given point of time, but no information about how many cells are secretors of the
cytokine.
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In ELISPOT there is possibility of detecting parallel secretion of 2 proteins by the
same cell
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In ELISA it is not possible to detect secretion of 2 proteins by the same cell parallely.

7. Read More on
• Bio-Resource
• http://www.technologyinscience.blogspot.co
m/2013/10/elispot-procedure-protocoladvantages.html