1. AKANKSHA PALIAL
palial.akanksha@gmail.com | 9717219879
Job objective
Seeking and challenging growth oriented assignment that commensurate with my knowledge and experience in
Healthcare, R&D and Quality Control.
Profile summary
 Total 5.8 years of work experience in microbiological research and quality control.
 Worked with Nestle India Ltd. Moga factory for about a year where I tested and inspected edible
products for food pathogens at various stages of the production process, in order to determine and
maintain the quality and reliability of the company’s product.
 Worked with Institute of microbial technology, Chandigarh under the project name "Biodiversity of
India's rich microbial diversity", where bio degradation of pesticides were studied using spectrometer
and high pressure liquid chromatography (HPLC).
 Worked with Veterinary College, Palampur in the area of microbiological research where herbal plant
extracts were prepared and checked for antimicrobial properties against clinical microorganism isolated
in laboratory.
 Antimicrobial property of various herbal extracts was checked by TEM (Transmission Electron
Microscopy), Liquid chromatography and Mass spectroscopy (LC-MS), culture sensitivity test (baur-kirby
test) and other in-vitro and in-vivo tests designed as per the requirement of the project.
 Have full papers published in distinguished journals and many national and internal articles/abstracts
published.
Key Skills
 Tested pathogens in finished products, line samples, culinary samples, raw materials and environmental
samples.
 Analysed & confirmed biochemical tests of bacteria (Salmonella, S. aureus, E. coli, Coliforms, yeast and
mold, B. cereus, Listeria monocytogenes, Shigella, C. botulinum, C. perfringens, flat Sours, Vibrio
cholera, Vibrio parahaemolyticus, and faecal streptococci)
 Analyzed water samples for biological impurities.
 Handled HPLC, GC, PCR, spectrophotometer, LC-MS and Transmission electron microscope. (TEM).
 Designed and standardized the protocols for the experiment.(in-vivo and in-vitro)
 Isolated field strains associated with skin and wound pathogens.
 Confirmed the field strains upto species level by performing the biochemical tests.
 Lyophilized extracts and cultures isolated.
 Prepared and fractionated plant extracts.

2.  Isolated and identified the bioactive compounds in plants through liquid chromatography and mass
spectroscopy.
Work Experience
College of Veterinary Sciences, Palampur (H.P)
Senior Research Fellow | January 2009- March 2013.
 Standardized the protocols for antibacterial and antifungal activity of sea buckthorn seed oil and leaf
extract.
 Isolated various microorganisms associated with skin and wound pathogens.
 Performed in-vitro and in-vivo experiments with standard and clinical isolates to determine the anti-bacterial
activity, taking rabbit as the experimental model.
 Prepared and fractionated the extracts from plant.
 Identified bioactive compounds through liquid chromatography and mass spectroscppy in plant parts and
studied their antimicrobial effect against the pathogenic clinical strains.
 Qualitatively and quantitatively determined the anti-microbial activity of the extracts.
 Transmission electron microscopy was performed to check the antimicrobial activity.
Institute of Microbial Technology, Chandigarh (U.T)
Project Assistant | July 2008 to Dec 2008
 Worked with Dr. Swarnjit Singh Cameotra (Scientist F) IMTECH, Chandigarh.
 Isolated, characterized and identified the microorganisms from soil contaminated with pesticides (4,4
dihydroxy biphenyl and biphenyl)
 Carried out the experiments related to biodegradation of pesticides by the isolated microorganisms and
confirming the biodegradation through High Performance Liquid Chromatography chromatograms.
Nestle India Ltd., Moga Factory, Punjab (CQAL)
Microbiologist | October 2006 to September 2007
 Undertook dilution preparation and analyzed the food samples as well as provided MIS data for Mini
Business Unit (MBU) meeting.
 Conducted internal & external audits to achieve quality standards as per the process benchmarks.
 Conducted tests under BIS (Bureau of Indian Standards)
 Charged with the daily closure of Samples.
 Coordinated with media department for daily media preparation.
 Daily checked and signed-off the pH of various prepared microbial media and maintained stock cards.
 Planned, assigned, managed and imparted training (regarding laboratory techniques) to the trainees.
 Maintained good lab practices (GLP) and safety within lab premise and conducted quality lab hygiene
checks.
Attainments
 Isolated many strains both fungal and bacterial from soil and clinical cases and preserved at -80°C
(bacterial) and fungal cultures in mineral oil at 4°C.
 Isolated Bacillus species and standardized its growth parameters.
 Handled spectrophotometer, HPLC, GC, PCR.

3.  Isolated bacteria growing in soil fortified with pesticides (4, 4 Dihydroxy biphenyle.)
 Determined experimentally the biodegradation of pesticides that contaminated the environment
seriously
 Quantitatively and qualitatively determined the antimicrobial activity of seabuckthorn plant parts.
 in-vitro and in-vivo experiments were done with clinical and standard pathogens.
 Culture sensitivity test (Bauer and Kirby) was done with different clinical isolates (bacterial and
fungal).
 Identified the bioactive components present in seabuckthorn plant responsible for its antimicrobial
activity through Liquid chromatography and mass spectroscopy.
 Antimicrobial activity was proved through Transmission Electron Microscopy (TEM) as well.
Academic Qualifications
MSc. Microbiology: Gharwal University2006Sardar Bhagwan Singh Post Graduate Institute of Bio-Medical
Sciences and Research (S.B.S.P.G.I.), Dehradun: 68.9%
BSc. Microbiology: Guru Nanak Dev University2004
Kanya Maha Vidyalaya, Jalandhar: 68%
XIIth: Himachal Pradesh Board of School Education 2001
Senior Secondary School, Dharamshala: 63%.
Xth: ICSE board: 63%
Projects Undertaken
Project Name I: Production of a Thermstable alpha-Amylase from Thermophilic Bacillus Species
Duration: January 2006 to June 2006
Project name II: Exploitation of India's Rich Microbial Diversity. (Biotransformation of Pesticides.)
Duration: July2008 to December 2008
Project name III: ‘in-vitro and in-vivo investigations on antimicrobial properties of sea buckthorn oil and
formulations and leaf extract against common bacteria and fungi associated with skin and
wound infections of animals.
Duration: January 2009 – March 2013
IT Proficiency : MS Office & Internet

4. Attendance in National and International Symposium
 Participated in one day Colloquium on “Animal diseases and Zoonoses in Northwestern Himalayas”
organized by the Indian Society for Veterinary Immunology and Biotechnology (ISVIB) held at Department
of Veterinary Microbiology, Dr.G.C.Negi College of Veterinary and Animal Sciences, Palampur, 19
September 2009.
 Participated in National conference on Sea buckthorn: Emerging Trends in Production to Consumption
(February, 16-18, 2010) organized by CSK HPKV, Palampur, and H.P.
 Participated in National conference on Seabuckthorn: Emerging Trends in R&D on Health Protection
&Environmental Conservation (December 1-3,2011)
 Participated in National Symposium on Nanobiotechnology-IIIT- Mandi (1-2 June 2012)
 Participated in National seminar on Indian Agriculture: Present Situation, Challenges, Remedies and Road
Map (4-5 August 2012)
Seminar Delivered
 Assessment of antibacterial activity of sea buckthorn (Hippophae rhamonides) seed oil against common
skin pathogens.
Research Articles National and International
National
 Mandeep Sharma, Akanksha Palial, Rajesh Chahota, Harshit Verma. “Assessment of antimicrobial
activity of Seabuckthorn seed oil against skin infections” Paper presented in the National Conference on
Seabuckthorn: Emerging trends in Production to Consumption held at CSK HPKV, Palampur from
16.2.2010 to 18.2.2010.
 Rajesh Chahota, Akanksha Palial, Harshit Verma and Mandeep Sharma “Evaluating antimicrobial
properties of seabuckthorn (Hippophae rhamnoides L.) seed oil against strains of Staphylococcus spp.
commonly involved in animal skin infections”. Paper presented in the Xth Indian Veterinary Congress and
XVII Annual Conference of Indian association for the Advancement of Veterinary Research (IAAVR) and
National Symposium on Challenges in Veterinry Reserch Education vis-à-vis safe animal, food and human

5. health, held at College of Veterinary Science and Animal Husbandry, MP Pashu Chikitsa Vigyan Vishwa
Vidyalaya, Jabalpur from 11.3.2010 to 12.3.2010.
 Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virendra Singh “Comparative Antimicrobial
Activities of Leaf Extracts of Hippophae rhamnoides and Hippophae salicifolia Species of Seabuckthorn
against Bacterial Pathogens Associated with Skin and Wound Infections of Livestock of Himachal
Pradesh”: ” Proceedings of National Conference on Seabuckthorn (Hippophae L.):Emerging Trends in
R&D ON Health Protection & Environmantal Conservation.(December 1-3,2011)
 Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virender Singh “Antibacterial activity of
seabuckthorn Hippophae salicifolia leaf extract against skin and wound pathogens of domestic animals
Sharma M, Chahota R, Palial A and Singh V. Antibacterial activity of seabuckthorn Hippophae salicifolia
leaf extract against skin and wound pathogens of domestic animals. XIX – Annual conference of
IndianAssociation for the advancement of Veterinary research (IAAVR) 20-21 April.
 Palial A, Chahota R, Sharma M, Verma S and Dhar P. Comparative Antibacterial Properties of Three
Landraces of Seabuckthorn (Hippophae L.) Found in in India against Methicillin Resistant (MRSA)
Staphylococcus aureus. National symposium on Nanobiotechnolo 1-2 June.
International
 Mandeep Sharma Rajesh Chahota and Akanksha Palial “Antimicrobial activity of sea buckthorn (Hippophae
Rhamnoides l.) seed oil against staphylococcus aureus strains isolated from skin infections of animal.”:
Seabuckthorn on the way between Sceience and Industry Interaction, Fourth International Seabuckthorn
Association Conference ,Belokuriha, Russia September 1-6, 2009
 Rajesh Chahota1, Mandeep Sharma1, Harshit Verma1, Akanksha Palial1, R.D. Patil2, Arvind Sharma3 and
N. P. Kuradey4 “Studies on effects of seabuckthorn (Hipphophae L.) Leaf extract and seed oil on infected
cutaneous wound healing process in rabbit experimental model”: Developing Seabuckthorn for the
Harmonious Ecosystem & Healthy Life, Fifth International Seabuckthorn Association Conference(ISA-Sept
4-9,2011)
 Mandeep Sharma1, Rajesh Chahota1, Harshit Verma1 and Akanksha Palial1 “Investigation of antibacterial
properties of seabuckthorn (Hippophae rhamnoides L.) leaf extracts against common skin and wound
microbial pathogens ”: Developing Seabuckthorn for the Harmonious Ecosystem & Healthy Life, Fifth
International Seabuckthorn Association Conference(ISA- Sept 4-9,2011)

6. Full Papers Published:
 Verma H, Chahota R, Palial A, Patil RD, Sharma A, Kuradey N.P, Sharma M. Effect of seabuckthorn
(Hipphophae L.) Leaf extract and seed oil on infected cutaneous wound healing process in rabbit model.
Indian Journal of Animal Sciences 82 (1): 54–00, January 2012
 Verma H, Chahota R, Palial A, Sharma M. Studies on antibacterial properties of seabuckthorn (Hippophae
rhamnoides L.) leaf extracts against common skin and wound microbial pathogens.. Indian Veterinary
Research Journal (vol.20,No.1-2011)
 Harshit Verma, Mandeep Sharma, Rajesh Chahota and Akanksha Palial Assessment of antimycotic
activity of seabuckthorn (Hippophae rhamnoides) leaf exacts against common fungi associated with skin
dermatitis: doi:10.5455/vetworld.2013.205-208
 Mandeep Sharma, Rajesh Chahota, Akanksha Palial and Virendra Singh “Comparative Antimicrobial
Activities of Leaf Extracts of Hippophae rhamnoides and Hippophae salicifolia Species of Seabuckthorn
against Bacterial Pathogens Associated with Skin and Wound Infections of Livestock of Himachal Pradesh”
Proceedings of National Conference on Seabuckthorn (Hippophae L.):Emerging Trends in R&D ON Health
Protection & Environmantal Conservation.(December 1-3,2011)
Personal Vitae
Date of Birth : 17th February, 1983
Permanent Address : House No. 1277, I2 Towers, Gaur Grandeur Appartments, Sec-119 Noida U.P-201301
Phone : 9717219879
References : Available on request.