1. Jung-Hyun Park, Yoon Mi Jin, Soojin Hwang, Du-Hyong
Cho, Duk-Hee Kang, Inho Jo
Carolina Mora Sánchez
Laura María Moreno León
2. Are a group of disorders of
the heart and blood vessels
Are the number one
cause of death globally
Can be prevented by
addressing risk factors.
>Coronary heart disease.
>Cerebrovascular disease.
5. Most uric acid dissolves in blood
and travels to the kidneys, where
it passes out in urine. We can use
uric acid levels in the diagnosis
different pathologies
Is the end product of purine
metabolism in humans
In normouricemic
humans, the daily
production and
ingestion of uric acid
is well balanced by
the daily excretion
7. eNOS is responsible
for most of the
vascular NO
produced.
That increased blood flow
by dilating blood vessels
Is a gas and is an important
protective molecule in the
vasculature
8. Dysregulation contributes
to the pathogenesis of
certain diseases Dimerization.
Interaction with calmodulin
(CaM) and
tetrahydrobiopterin (BH4),
Acetylation
Phosphorylation.
eNOS is regulated by
9. Mediates the control of a large
number of enzymes, ion channels
and other proteins by Ca2+.
Is a calcium-binding
messenger protein
expressed in all
eukaryotic cells.
Among the enzymes to be stimulated by
the calmodulin-Ca2+ complex are a number
of protein kinases and phosphatases.
10. Bovine aortic endothelial cells (BAEC)
Human umbilical vein EC (HUVEC)
Human plasma and aortic EC – in vitro
Pulmonary artery EC
11. eNOS CaM in EC
eNOS activity
NO production
Reducing the
binding between
12. Explain how uric acid
decreases NO production
by direct modulations on
eNOS, leading to causing
cardiovascular problems.
13. Ácido úrico
eONS bovina recombinante
eNOS, p-eNOS-Thr 495, p-eNOS-Ser1177, p-eNOS- Ser114
Calmodulina recombinanteFluo-4 AM
Ig CaM
FBS
M200Ig Lisina acetilada
LSGSDPBS NCSL-glutamina
Penicilina y estreptomicina
MEM
Tripsina EDTA
14. Método para el estudio del
comportamiento de las células, muy
adecuado como modelo para el estudio
del desarrollo y la diferenciación, por lo
que las líneas celulares que conservan
la capacidad de diferenciarse
15. La producción de ON es medida como concentración de
nitrito en la células cultivadas.
16. La cuantificación de la actividad de eNOS se
lleva a cabo midiendo la conversión de L-arginina
en L citrulina, en presencia de concentraciones
saturantes de los cofactores enzimáticos
adecuados.
18. Proteínas procedentes de extractos celulares son
separados por electroforesis en gel según su tamaño kDa
Técnica: SDS-PAGE policrámida en
la que son disueltas en una solución,
con el detergente sodio duodecil
sulfato.
19. Manipulación que se hace con
células o sistemas aislados de
células, en el laboratorio con los que
se trata de simular las condiciones
que tendrían en los organismo de los
que fueron aislados
20. Es una técnica popular para identificar
las interacciones proteína-proteína
fisiológicamente relevantes mediante el
uso de anticuerpos para determinar la
presencia de una proteína específica.
22. Es una enzima del ciclo de la urea que
cataliza la hidrólisis de la arginina,
produciendo ornitina y urea.
23. Amplificación de RNA a través de la
síntesis previa de su cDNA (DNA copia),
utilizando una transcriptasa inversa,
seguida de varios ciclos de PCR
convencional.
Determinar
expresión genética
en diversos tejidos.
29. RESEARCHER COMMENT AGREE DISAGREE
D.I Feing
¨Epidemiology
studies showed
that higher blood
level of uric
acid is significantly
associated with
the risk of
cardiovascular
diseases¨.
X
30. RESEARCH
ER
COMMENT AGREE DISAGREE
I.
Papeziková
¨Most recent
study revealed
that uric acid
decreased NO
production, both
indirectly
and directly; by
increasing
arginase activity
and decreasing
eNOS-Ser1177
phosphorylation,
respectively¨.
X
31. RESEARCHER COMMENT AGREE DISAGREE
C. Nathan
¨CaM is one of
the key proteins
for eNOS
activation.
Although
CaM binds
tightly to eNOS,
even at the
resting basal
levels of
intracellular
Ca2+ levels¨.
X
32. RESEARCHER COMMENT AGREE DISAGREE
J. White
¨increased
intracellular Ca2+
by
extrastimuli such
as ionomycin
induced more
association of
CaM
with eNOS¨.
X
33. • Uric acid inhibits the production of nitric oxide which is
a vasodilator important for the prevention of heart
disease
• This project can help people to understand how if they
do not have healthy habits can cause cardiovascular
disease.
34. • Ionomycin is used in research to raise the intracellular
level of calcium, this help the interaction between
eNOs y CaM.
• Uric acid reduced the interaction between CaM and
eNOS this produces a disminusion of the production of
NO.