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ISEST 2009, Shanghai, June 4, 2009 Bart De Gusseme B. Pycke, T. Hennebel, A. Marcoen,  S. E. Vlaeminck, H. Noppe, N. Boon, W.  Verstraete Laboratory of Microbial Ecology & Technology, Ghent University, Belgium ,[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
Introduction ,[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
17α-ethinylestradiol ,[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
Need for effluent post-treatment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
Need for effluent post-treatment ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 Need for alternative and sustainable WWTP effluent polishing techniques
Nitrifying cultures and EE2 removal ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
Research questions ,[object Object],[object Object],[object Object],Commercially available NEC (ABIL) (Grommen et al., 2002; Aquacult, 211: 115-124; LabMET) AOB:  Nitrosomonas NOB:  Nitrobacter  &  Nitrospira Heterotrophic strains Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
1. Batch incubation experiments ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
1. Batch incubation experiments Heat-treated NEC B iomass-free  control Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 NEC in WWTP effluent 9 μg EE2/g VSS.h NEC in minimal medium 9 μg EE2/g VSS.h NH 4 + -N : 52.5    < 2 mg/L
[object Object],1. Batch incubation experiments Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],[object Object]
2. Mechanism ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
2. Mechanism Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 EE2 removal ----  Nitrate production 96    1 % removal without ATU Complete nitrification 38    1 % removal with ATU No nitrification ,[object Object]
3. Continuous EE2 removal in MBR A. Reactor design Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 Influent  (tap water containing EE2) Plate membranes Effluent Gas diffuser Sprue with vacuum Air
3. Continuous EE2 removal in MBR B. Operational parameters ,[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 100 μg/L 83 ng/L
3. Continuous EE2 removal in MBR C. Reactor performance Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],[object Object]
Future perspectives Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009 ,[object Object],[object Object],Discharge to surface waters Add-on MBR with NEC Wastewater Treatment Plant Effluent Activated sludge  If necessary, d osage of NH 4 + -N to 1 mg/L
Conclusions ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Siegfried E. Vlaeminck (Aspirant) are supported by the  Fund of Scientific Research (FWO)-Flanders Benny Pycke is supported by a PhD grant (IWT-SB/53360) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT) This study was part of the EU Neptune project (ContractNo. 036845, SUSTDEV-2005-3.II.3.2), which was financed by the EU Commission and the Sixth Framework (FP6-2005-Global-4). Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009
B. De Gusseme, B. Pycke, T. Hennebel, A. Marcoen, S. E. Vlaeminck, H. Noppe, N. Boon & W. Verstraete ,[object Object],[email_address] LabMET.UGent.be Bart De Gusseme  An add-on MBR as effluent polishing technique for 17α-ethinylestradiol  ISEST 2009, Shanghai, June 4, 2009

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An Add On Nitrifying MBR As Effluent Polishing Technique For Ee2

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  • 9. 1. Batch incubation experiments Heat-treated NEC B iomass-free control Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 NEC in WWTP effluent 9 μg EE2/g VSS.h NEC in minimal medium 9 μg EE2/g VSS.h NH 4 + -N : 52.5  < 2 mg/L
  • 10.
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  • 13. 3. Continuous EE2 removal in MBR A. Reactor design Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009 Influent (tap water containing EE2) Plate membranes Effluent Gas diffuser Sprue with vacuum Air
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  • 18. Acknowledgements Bart De Gusseme (Aspirant), Tom Hennebel (project no. 7741-02) and Siegfried E. Vlaeminck (Aspirant) are supported by the Fund of Scientific Research (FWO)-Flanders Benny Pycke is supported by a PhD grant (IWT-SB/53360) of the Institute for the Promotion of Innovation through Science and Technology in Flanders (IWT) This study was part of the EU Neptune project (ContractNo. 036845, SUSTDEV-2005-3.II.3.2), which was financed by the EU Commission and the Sixth Framework (FP6-2005-Global-4). Bart De Gusseme An add-on MBR as effluent polishing technique for 17α-ethinylestradiol ISEST 2009, Shanghai, June 4, 2009
  • 19.