Analysis, design, implementation and testing of an optoelectronic system with a high power infared laser diode for cancer therapy using gold nanoparticles. Animal (murine) model.

1. Energy-based Treatment of Tissue and Assessment VI
Modelling and characterization of photothermal
effects assisted with gold nanorods in ex-vivo
samples and in a murine model
AUTHORS:
Félix Rodríguez Jara1, Horacio Lamela Rivera2 and
Vincent Cunningham3
1felix.rjara@alumnos.uc3m.es
2horacio@ing.uc3m.es
3vcunning.uc3m.es
OPTOELECTRONICS AND LASER TECHNOLOGY GROUP
ELECTRONIC TECHNOLOGY DEPARTMENT
San Francisco (CA), January 23th, 2011

2. 0. General Index.
1. Introduction
General Index:
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
1. Introduction
2. Opto-thermal
modelling for
photo-thermal 2. Opto-thermal modelling for Photo-Thermal Therapy
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental Results
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
4. Conclusions and Future Work
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
Modelling and characterization of photothermal effects assisted with
1 gold nanorods in ex-vivo samples and in a murine model

3. 0. General Index.
1. Introduction
General Index:
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
1. Introduction
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
Modelling and characterization of photothermal effects assisted with
2 gold nanorods in ex-vivo samples and in a murine model

4. 0. General Index.
1. Introduction
1.1 The Motivation.
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system. CANCER, one of the main causes of mortality all around the world.
1.4 Nanotechnology
in Photo-thermal
therapy.
- 7.4 Millions out of the total deaths per year ≈ 13 % (WHO)
1.5 The Design.
2. Opto-thermal
modelling for Need of investigation in new therapy techniques
photo-thermal
therapy. Mortality and side effects
2.1 Approach
2.2 Finite Element
Modelling.
Number of patients that can be trated
3. Experimental
Results. Laser hyperthermia technique
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo Collaboration with a specialized Company in
4. Conclusions and Animal models
Future Work
- Good Laboratory Practices (GLP)
- Qualified Staff
- Ethical Committee
Modelling and characterization of photothermal effects assisted with
3 gold nanorods in ex-vivo samples and in a murine model

5. 0. General Index.
1. Introduction
1.2 The hyperthermia technique: the GOAL
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
Hyperthermia temperature Tumoral Cell death
therapy. (from 42ºC to 65 ºC)
1.5 The Design.
held during various minutes
2. Opto-thermal GOAL: 80% Tumour
(3-10’)
modelling for
photo-thermal
tissue ablation
therapy.
2.1 Approach
2.2 Finite Element
Modelling. 42 ºC – 65 ºC
3. Experimental
Results. Tumour
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
37 ºC
Results – in-vivo
5-10 mm
4. Conclusions and
Future Work
Biological Tissue
Hyperthermia temperature selected:
55 ºC
ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA
4 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO

6. 0. General Index.
1.2 The Hyperthermia Technique: State-of-
1. Introduction
1.1 The Motivation The-Art
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy. Registro Tª
1.5 The Design. Láseres alta potencia
estado sólido (Nd:YAG, OPO)
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach Radiofrecuencia
2.2 Finite Element
Modelling. Ultrasonidos (HIFU)
3. Experimental
Fibra óptica + Difusor
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Tumor
Results – in-vivo
4. Conclusions and Tejido biológico
Future Work
- Low selectivity
- Use high amounts of energy
- Very expensive devices with big dimensions
Modelling and characterization of photothermal effects assisted with
5 gold nanorods in ex-vivo samples and in a murine model

7. 0. General Index.
1. Introduction
1.3 The Photo-thermal Therapy System
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology HIGH
in Photo-thermal POWER
therapy. LASER
1.5 The Design.
DIODE
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element Temperature
Modelling. Register
3. Experimental Superficial
Results.
λ= 808
3.1 Experimental Set-
nm
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
Nanoparticle
Interna infusion:
4. Conclusions and Intratumoural or
Future Work Intravenous ?
Biological
Tissue
Modelling and characterization of photothermal effects assisted with
6 gold nanorods in ex-vivo samples and in a murine model

8. 0. General Index.
1. Introduction
1.4 Nanotechnology in Photo-Thermal Therapy
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
Gold Nanoparticles
GOLD NANORODS
in Photo-thermal
therapy.
1.5 The Design. Tuned optical
EFFICIENCY POWER
2. Opto-thermal absorbance
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo Fig. adapted of “Cancer Research”, 69(9):1-9, (2009)
3.3 Experimental
Results – in-vivo 1.5
- Passive A A Longitudinal
4. Conclusions and
Surface Plasmon
Future Work
- Harmless
1
Absorción [cm -1]
Resonance Peak
B B Axial
0.5
Surface Plasmon
Resonance Peak
0
400 500 600 700 800 900 1000 1100
Longitud de onda [nm]
Modelling and characterization of photothermal effects assisted with
7 gold nanorods in ex-vivo samples and in a murine model

9. 0. General Index.
1. Introduction
1.5 The Design: Experimental studies
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy. Efficiency Study Efficacy Study
2.1 Approach
2.2 Finite Element
Modelling.
CT-26 COLON CANCER
OPTIMIZE THE SET-UP
3. Experimental XENOGRAFT STUDIES
Results.
3.1 Experimental Set- - Number of animales for group -> Statistics - Tumour size?
up.
3.2 Experimental
Results – ex-vivo
- System design in terms of efficiency: - Stops Tumor growing?
3.3 Experimental
Results – in-vivo (Power, Irradiance, Nanoparticle Concentration) - Ablation of the tumoral tissue?
4. Conclusions and - Therapy parameters
Future Work
(Exposure time, way of application)
- Ethical Committee (international directives of
animal handling) -> HEATING UP, NOT BURNING
OR CHARRRING
ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA
8 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO

10. 0. General Index.
1. Introduction
General Index:
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
2. Opto-thermal Modelling for
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Photo-Thermal Therapy
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA
10 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO

11. 0. General Index.
1. Introduction
2.1 Approach
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
Energy
Optical power, Temperature
1.5 The Design. Deposition Transferencia
Irradiance Fuentes térmicas
térmica
2. Opto-thermal P [W], I [W/cm2]
modelling for
photo-thermal
therapy. Optical properties Physical and thermal properties
2.1 Approach of the tissue of the tissue
2.2 Finite Element
Modelling.
µa [cm-1], µs [cm-1] ρ[kg/m ]C [JKg-1K-1, k [Wm-1K-1],
3
3. Experimental
Results. Thermal Source
3.1 Experimental Set-
up.
3.2 Experimental Optical Power
Results – ex-vivo
3.3 Experimental Thermal Energy
Results – in-vivo Transferred
Temp
4. Conclusions and
Future Work dV dV
Tissue absorption + Nanoparticles
µtotal = µtissue+µnanoparticles
Modelling and characterization of photothermal effects assisted with
11 gold nanorods in ex-vivo samples and in a murine model

12. 0. General Index.
1. Introduction
2.2 Finite Element Modelling (FEM)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system. - Less computational resources needed
1.4 Nanotechnology
in Photo-thermal
- It can be applied to complex geometries
therapy.
1.5 The Design.
2. Opto-thermal
modelling for Energetic
Initial Thermal
photo-thermal Contribution.
Temperature Energy
therapy. Laser energy
37 ºC transference
2.1 Approach absorption
2.2 Finite Element
Modelling.
3. Experimental
NO
Results.
3.1 Experimental Set- Stop time Temperatures
up.
3.2 Experimental reached? update
Results – ex-vivo
3.3 Experimental
Results – in-vivo
YES
4. Conclusions and
Future Work
Final
Temperature
Modelling and characterization of photothermal effects assisted with
14 gold nanorods in ex-vivo samples and in a murine model

13. 0. General Index.
1. Introduction
General Index:
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
3. Experimental Results
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
15

14. 0. General Index.
1. Introduction
3.1 Experimental Set-up (I)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
Optoelectronic General Schema
1.4 Nanotechnology Thermocouple
in Photo-thermal
therapy.
thermometer
1.5 The Design.
Sonda termopar
2. Opto-thermal
Lentes de hipodérmica
modelling for
photo-thermal Cabezal láser acoplo (HYP-1, Omega)
Soporte
therapy. (CNI-MDL-H-808-5000, CNI) d1
2.1 Approach
2.2 Finite Element Diámetro del haz
Modelling.
(FWHM)
d0
3. Experimental Fibra óptica
d2
Results. l1 l2
3.1 Experimental Set-
(CNI-SMA-Fibre-600, CNI)
up.
3.2 Experimental
Results – ex-vivo
l1 = l2 Tejido irradiado
3.3 Experimental f1= f2 = 2.54 cm
Results – in-vivo D1 = D2= 2.54 cm
Termómetro infrarrojo
4. Conclusions and
(OS-530LE, Omega)
Future Work
Driver
(PSU-H-LED, CNI)
Modelling and characterization of photothermal effects assisted with
16 gold nanorods in ex-vivo samples and in a murine model

15. 0. General Index.
1. Introduction
3.1 Experimental Set-up (II)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
Experimental Set-up for therapy application in ex-vivo tissue samples
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
Modelling and characterization of photothermal effects assisted with
17 gold nanorods in ex-vivo samples and in a murine model

16. 0. General Index.
1. Introduction
3.1 Experimental Set-up (and III)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
Experimental set-up for therapy application in mice (in-vivo)
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
Modelling and characterization of photothermal effects assisted with
18 gold nanorods in ex-vivo samples and in a murine model

17. 0. General Index.
3.1 Experimental Set-up:
1. Introduction
1.1 The Motivation Development Stages
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
Develpment Stages of the Photo-Thermal Therapy System
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy. Requirements
1.5 The Design. and Goals Optimization loop
Optimization
of the
2. Opto-thermal
System
modelling for
photo-thermal
therapy.
2.1 Approach Concept, Design Biological Model
2.2 Finite Element Experimental
and Implementation ex-vivo
Modelling.
of the system (tissue samples) Results
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
Experimental Biological Model
3.3 Experimental in-vivo
Results – in-vivo Results (ratones)
4. Conclusions and
Future Work
Optimization
Clinical
of the
Application System
(Human Beings)
Modelling and characterization of photothermal effects assisted with
19 gold nanorods in ex-vivo samples and in a murine model

18. 0. General Index.
1. Introduction
3.2 Experimental results - ex-vivo (I)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
Sample preparation:
1.4 Nanotechnology
in Photo-thermal - Fresh chicken muscle tissue.
therapy.
1.5 The Design.
- Previous marking for infrared thermometer alineation.
2. Opto-thermal
modelling for - Hypodermical infusion of nanoparticles (Ntracker 30-PM-850, NANOPARTz,
photo-thermal saline solution PH = 7.4, 0.1 ml, OD = 25).
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
Modelling and characterization of photothermal effects assisted with
20 gold nanorods in ex-vivo samples and in a murine model

19. 0. General Index.
1. Introduction
3.2 Experimental results - ex-vivo (II)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
Looking for the optimal irradiance, experimental results.
1.4 Nanotechnology
in Photo-thermal
therapy. 55
Pbeam = 1.25 W
1.5 The Design. Phaz = 0.5 W, IFWHM = 0.95 [W/cm2], Láser+NRds
Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Láser+NRds
2. Opto-thermal
50 Phaz = 0.75 W, IFWHM = 1.43 [W/cm2], Láser+NRds
IFWHM = 2.38 W/cm2
Phaz = 0.5 W, FWHM = 0.95 [W/cm2
Phaz = 1.00 W, IFWHM =I 1.90 [W/cm2], Láser+NRds ], Solo Láser
modelling for 40
Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Láser+NRds 2
ΔT = 31 ºC
45
Phaz = 0.75 W, IFWHM = 1.43 [W/cm ], Solo Láser
photo-thermal Phaz = 0.5 W, IFWHM = 0.95 [W/cm2], Solo Láser
 T [ºC]
therapy. 40 Phaz = 1.00 W, IFWHM = ], Solo Láser 2
Phaz = 0.75 W, IFWHM = 1.43 [W/cm21.90 [W/cm ], Solo Láser
35
2.1 Approach Phaz = 1.00 W, IFWHM = 1.90 [W/cm22.38 [W/cm2], Solo Láser
Phaz = 1.25 W, IFWHM = ], Solo Láser
2.2 Finite Element 35
Phaz = 1.25 W, IFWHM = 2.38 [W/cm2], Solo Láser
Modelling.
30
30
3. Experimental
Results. 25 25
3.1 Experimental Set-
 T [ºC]
up. 20
3.2 Experimental 20
50 100 150 200 250 300
Results – ex-vivo Tiempo de exposición [sg]
3.3 Experimental
Results – in-vivo
15
4. Conclusions and
Future Work 10
ΔT = 3 ºC
5
0
0 50 100 150 200 250 300
Tiempo de exposición [sg]
Exposure Time [s]
Modelling and characterization of photothermal effects assisted with
22 gold nanorods in ex-vivo samples and in a murine model

20. 0. General Index.
1. Introduction
3.3 Experimental Results - in-vivo (I)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system. Animal model
1.4 Nanotechnology
in Photo-thermal - Female mice, albines, BALB/c (BALB/cAnNHsd) , specific patogens free.
therapy.
1.5 The Design.
- Animals were handled by qualified staff in a company with the Good Laboratory Practice Certificate
(GLP)
2. Opto-thermal
modelling for Animal preparation
photo-thermal
therapy. - Random distribution by weigth.
2.1 Approach
- Identification of each animal.
2.2 Finite Element
Modelling.
Identification
- Hair removing from the exposed area. marks Shaving of
3. Experimental - Light anaesthesya Ketamine/Xilacine (10μl/g). irradiated area
Results.
3.1 Experimental Set- - Hypodermic infusion of nanoparticles
up.
3.2 Experimental (Gold Nanorods, Ntracker).
Results – ex-vivo
3.3 Experimental - Laser irradiation exposure.
Results – in-vivo
4. Conclusions and
Positioning
Future Work platform
Laser beam
direction
27 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

21. 0. General Index.
1. Introduction
3.3 Experimental Results - in-vivo (II)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
Study of the thermal increment induced as a function of laser irradiance.
therapy system.
1.4 Nanotechnology
in Photo-thermal
Pbeam = 1.25 W
therapy.
1.5 The Design.
35
IFWHM = 2.38 W/cm2
HYP: P = 0.5 W
2. Opto-thermal
modelling for
SUP: P = 0.5 W
HYP: P = 1 W
ΔT = 29.9 ºC
30
photo-thermal SUP: P = 1 W
therapy. HYP: P = 1.25 W
2.1 Approach SUP: P = 1.25 W
25
2.2 Finite Element
Modelling.
3. Experimental 20
 T [ºC]
Results.
3.1 Experimental Set-
up.
3.2 Experimental
15
12 ºC
Results – ex-vivo
3.3 Experimental
Results – in-vivo 10
4. Conclusions and
Future Work 5
0
0 50 100 150 200 240
Tiempo de exposición [sg]
Exposure Time [s]
28 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

22. 0. General Index.
1. Introduction
3.3 Experimental Results - in-vivo (III)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal Proof of concept, Phaz = 1.25 W
therapy system.
experimental results.
1.4 Nanotechnology
in Photo-thermal
IFWHM = 2.38 W/cm2
therapy.
1.5 The Design.
60 T = 57.8 ºC
2. Opto-thermal
modelling for
55
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
50
Modelling.
Temperatura [ºC]
Temperature [ºC]
3. Experimental 45
Results.
3.1 Experimental Set-
T = 38.1 ºC
up.
40
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
35
4. Conclusions and HYP: Gold Nanorods + Láser
Future Work SUP: Gold Nanorods + Láser
30 HYP: PBS + Láser
SUP: PBS + Láser
25
0 100 200 300 400 500 600
Tiempo de exposición [sg]
Exposure time [sg]
33 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

23. 0. General Index.
1. Introduction
3.3 Experimental Results - in-vivo (IV)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal Aspect of the skin after irradiation.
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for Skin exposed to the laser
photo-thermal Superficiebeam expuesta
de piel
therapy. a la radiación láser
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
Results.
3.1 Experimental Set-
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
34 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

24. 0. General Index.
1. Introduction
3.3 Experimental Results - in-vivo (V)
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal Proof of concept, FEM
therapy system.
1.4 Nanotechnology Modelling.
in Photo-thermal
therapy.
1.5 The Design. 16
2. Opto-thermal 14
modelling for
photo-thermal
12
therapy.
2.1 Approach
2.2 Finite Element
10
Modelling.
 T [ºC]
3. Experimental 8
The superficial thermal gradient registered
Results.
3.1 Experimental Set-
experimentally was of 14.75 ºC, while the
up.
3.2 Experimental
6
modelled one was of 14.86. This is an absolute
Results – ex-vivo
3.3 Experimental 4
error less than 0.11 ºC (0.75 %)
Results – in-vivo
Superficial. Laser + NRds. EXP
4. Conclusions and 2
Superficial. Laser + NRds. FEM
Future Work
0
0 100 200 300 400 500 600
Exposure exposición [sg]
Tiempo de
time [sg]
35 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

25. 0. General Index.
1. Introduction
General Index:
1.1 The Motivation
1.2 The hyperthermia
technique.
1.3 The Photo-thermal
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
1.5 The Design.
2. Opto-thermal
modelling for
photo-thermal
therapy.
2.1 Approach
2.2 Finite Element
Modelling.
3. Experimental
4. Conclusions and future
Results.
3.1 Experimental Set-
up.
3.2 Experimental
work
Results – ex-vivo
3.3 Experimental
Results – in-vivo
4. Conclusions and
Future Work
37 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

26. 0. General Index.
1. Introduction
4.1 Conclusions:
1.1 Motivation
1.2 The hyperthermia
technique
1.3 Photo-thermal The laser hyperthermia therapy system using gold nanoparticles
therapy system.
1.4 Nanotechnology has been demonstrated to be VIABLE in a mice model.
in Photo-thermal
therapy.
1.5 Design.
Only the tissue injected with nanoparticles reaches hyperthermia
2. Opto-thermal temperatures.
modelling for
photo-thermal
therapy.
2.1 Approach
The tissue exposed to the laser beam, but NO injected with
2.2 Energy Balance nanoparticles, remains unaltered (it does not reach hyperthermia
2.3 Implemention of the
solution. FEM.
2.4 Finite Element
temperature)
Modelling.
3. Experimental The stablished irradiance does not induce tissue charring or skin
Results.
3.1 Experimental Set-
burning.
up.
3.2 Experimental
Results – ex-vivo
3.3 Experimental
The computational model implemented allows to make accurate
Results – in-vivo
estimations of the final temperature of the irradiated tissue with
4. Conclusions and nanoparticles.
Future Work
38 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

27. 0. General Index.
1. Introduction
4.2 Future work
1.1 Motivation
1.2 The hyperthermia
technique
1.3 Photo-thermal
Modelling and implementation
therapy system.
1.4 Nanotechnology
in Photo-thermal
therapy.
Study and design of new techniques of optical energy irradiation:
1.5 Design. contact applicators, more than one laser source, pulsed light.
2. Opto-thermal
modelling for
Design and test of an integrated control and monitorization
photo-thermal
therapy. system:
2.1 Approach Tissue Temp.
2.2 Energy Balance temperature register
2.3 Implemention of the
solution. FEM.
2.4 Finite Element
Modelling.
Laser power
3. Experimental
Results.
3.1 Experimental Set-
up. Experimental study
3.2 Experimental
Results – ex-vivo
3.3 Experimental
Results – in-vivo Tumor model in mice to test the efficacy of the system
4. Conclusions and -Determine if the tumour growing is stopped and finally, the tumour is
Future Work ablated.
- 15 animals (BALB/c)
- Cell line CT-26 mice colon cancer (CT26.WT)
39 Modelling and characterization of photothermal effects assisted with
gold nanorods in ex-vivo samples and in a murine model

28. Thank you for your attention!
Have a nice day.
ESTUDIO EXPERIMENTAL DE TÉCNICAS LÁSER PARA TERAPIA
40 CÁNCER EN RATONES UTILIZANDO NANOPARTÍCULAS DE ORO