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Welcome to the Community College Program DayBIO 2011Washington D.C.
PREPARING STUDENTS FOR NEW OPPORTUNITIES IN STEM CELL TECHNOLOGIES Tori Barron Lisa Seidman Madison Area Technical College Community College Program at BIO 2011
OVERVIEW Rationale; why this program? Programmatic issues Curriculum
RATIONALE Meeting needs of students Meeting needs of local employers
PROGAMMATIC Resources Expertise Equipment Space Students Curriculum and instructional materials Institutional support, grant support, community support
Human Embryonic Stem Cell Culture MethodsCurriculum Development ,[object Object]
M,W,TR (6:30-9:15pm)
Needs of local industry and research communities
Strong aseptic technique
Stem cell culture experience

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Ccp2011 4 seidman

Editor's Notes

  1. Challenging laboratory math, Separate aliquots for each studentStudents learn two culture systems most common in industry and researchSequencing of preparation and timing are keyPassaging and maintenance of hESCsUnderstanding when to split cells and at what densityPhotographic microscopy to assess confluency, morphology and differentiationPicking hood to remove differentiationFreezing and Thawing of hESCsLive cells to start, then freezing prior to holidayStudents thaw own frozen cultures post-breakUnderstanding when to split cells and at what densityPhotographic microscopy to assess confluency, morphology and differentiationPicking hood to remove differentiationUnderstanding when to split cells and at what densityPhotographic microscopy to assess confluency, morphology and differentiationPicking hood to remove differentiation
  2. , metaphase harvestDAPI Chromosomes, fluorescent microscopyScientific communication skills and reinforcement of course material through project