Anoxia: High expansion foam The Anoxia method is unique for creating an environment without oxygen under atmospheric circumstances. High expansion foam is produced by mixing nitrogen and a mixture of water and specially developed high expansion detergent, with an expansion rate upto 1:1000, meaning that 1 litre of water/foam agent mix expands up to 1 m3 foam. Due to the specially designed foam generator, the high expansion foam bubbles are filled with a > 99% concentration of nitrogen. The oxygen level surrounding the animal drops from 21% in atmospheric air to < 1 % once the animal is submerged in the foam. Anoxia: convulsions, but no stress or pain The animals need a constant supply of oxygen to the brain. Applying Anoxia foam, the oxygen is replaced by nitrogen. As a result the nitrogen level is raised to > 99% and the oxygen level is lowered to < 1%. Considering the natural reaction to sudden lack of oxygen the animal is rendering quickly into unconsciousness. As a consequence, behavioral indicators like loss of posture and convulsions will appear. With this in mind, unconscious animals are insensitive to perceive unpleasant sensations like pain. Anoxia: How Anoxia foam is created A mixture of 97% water and 3% high expansion foam agent is sprayed into the Anoxia foam generator, creating a thin film on the outlet of the generator. At the same time, nitrogen is added with overpressure into the foam generator. The nitrogen expands when it exits the generator, creating robust high expansion foam. The high expansion foam bubbles are filled with > 99% nitrogen. Anoxia: Single foam generator systems In practice, one Anoxia foam generator creates a volume of up to 750 liter of high expansion foam per minute. This volume is more than sufficient to fill a wheelie-bin container within 30 seconds. The most common container volumes are: M size - 240 liter; L size - 340 liter; and XL size - 370 liter. The choice of the volume of the container depends of the size of the animal and/or the number of animals that need to be stunned/killed. A lid with a chiffon that seals the container. As soon as the foam exits the chiffon, the gas supply is stopped and the chiffon is closed. The nitrogen gas concentration in the container remains at 99%.

1. www.anoxia.eu
Presentation 3
The Anoxia technique:
Small-scale Anoxia applications
For more information: www.anoxia.eu

2. Anoxia Applying the technique
Presented by the N2GF team
For more information: www.anoxia.eu

3. Anoxia: High expansion foam
– The Anoxia method is unique for creating an
environment without oxygen under atmospheric
circumstances
– High expansion foam is produced by mixing
nitrogen and a mixture of water and specially
developed high expansion detergent, with an
expansion rate upto 1:1000, meaning that 1 litre
of water/foam agent mix expands up to 1 m3
foam.
– Due to the specially designed foam generator, the
high expansion foam bubbles are filled with a >
99% concentration of nitrogen
– The oxygen level surrounding the animal drops
from 21% in atmospheric air to < 1 % once the
animal is submerged in the foam
3
For more information: www.anoxia.eu

4. • Atmospheric air contains 21% oxygen, 78% nitrogen and 1%
other gasses, mainly carbon dioxide; the animal needs a
constant supply of oxygen to the brain
• Applying Anoxia foam, the oxygen is replaced by nitrogen,
raising the nitrogen level to > 99% and lowering the oxygen
level to < 1%
• The natural reaction to sudden lack of oxygen is rendering
quickly into unconsciousness, causing behavioural indicators
like loss of posture and convulsions
• Unconscious animals are insensitive to perceive unpleasant
sensations like pain
Anoxia: convulsions, but no stress or pain
For more information: www.anoxia.eu

5. Anoxia: How Anoxia foam is created
• A mixture of 97% water and 3% high expansion foam
agent is sprayed into the Anoxia foam generator, creating
a thin film on the outlet of the generator
• At the same time, nitrogen is added with overpressure
into the foam generator
• The nitrogen expands when it exits the generator,
creating robust high expansion foam
• The high expansion foam bubbles are filled with > 99%
nitrogen
For more information: www.anoxia.eu

6. Anoxia: Single foam generator systems
• In practice, one Anoxia foam generator creates a volume
of up to 750 liter of high expansion foam per minute
• This volume is more than sufficient to fill a wheelie-bin
container within 30 seconds
• The most common container volumes are: M size - 240
liter; L size - 340 liter; and XL size - 370 liter
• The choice of the volume of the container depends of the
size of the animal and/or the number of animals that
need to be stunned/killed
• A lid with a chiffon that seals the container
• As soon as the foam exits the chiffon, the gas supply is
stopped and the chiffon is closed. The nitrogen gas
concentration in the container remains at 99%
For more information: www.anoxia.eu

7. 7
For more information: www.anoxia.eu
Anoxia: Capacity of the M size container system
Farm type
• Small-size Pig production farm
• Small-size free-range poultry/ duck/ geese farm
Consumption
• Gas consumption per filling; 240 liter / 19 sec
• Detergent consumption per filling: 0,01 liter
Capacity
• 5 piglets per filling 0 – 2 days old, average weight 1,35 kg
• 2 birds per filling average weight 2,5 kg
• 1 bird per filling average weight 6 kg

8. 8
For more information: www.anoxia.eu
Anoxia: Operational costs of the M size system
Investment M-size system ex costs: € 3,500 ex VAT
Running costs:
Pig growing farm
• 1 x per week – 5 piglets per filling; € 0,59 per piglet
• 5 x per week – 5 piglets per filling; € 0,34 per piglet
Free-range poultry farm, using bottled gas
• 1 x per week; € 2,94 per week
• 5 x per week; € 8,47 per week

9. 9
For more information: www.anoxia.eu
Anoxia: Capacity of the L size system
Farm type
• Medium-size Pig production farm
• Medium-size poultry/ duck/ gees farm
Consumption
• Gas consumption per filling; 340 liter / 27 sec
• Detergent consumption per filling: 0,02 liter
Capacity
• 10 piglets per filling 0 –2 days old, average weight 1,35 kg)
• 3 birds per filling average weight 2,5 kg
• 1 bird per filling average weight 6 kg – 10 kg

10. 10
For more information: www.anoxia.eu
Anoxia: Operational costs of the L size container
system
Investment foam generator ex costs: € 3,900 ex VAT
Running costs:
Pig growing farm
• 1 x per week – 10 piglets per filling; € 0,30 per piglet
• 5 x per week – 10 piglets per filling; € 0,24 per piglet
Free-range poultry farm, using bottled gas
• 1 x per week; € 3,00 per week
• 5 x per week; € 11,95 per week

11. 11
For more information: www.anoxia.eu
Anoxia: Capacity of the XL size container system
Farm type
• Large-size Pig production farm
• Large-size poultry/ duck/ gees farm
Consumption
• Gas consumption per filling; 380 liter / 30 sec
• Detergent consumption per filling: 0,02 liter
Capacity
• 15 piglets per filling 0 – 2 days old, average weight 1,35 kg
• 4 birds per filling average weight 2,5 kg
• 2 bird per filling average weight 6 kg
• 1 bird per filling average weight 10 kg

12. 12
For more information: www.anoxia.eu
Anoxia: Operational costs of the XL size system
Investment foam generator ex costs: € 4,200 ex VAT
Running costs:
Pig growing farm
• 1 x per week –15 piglets per filling; € 0,27 per piglet
• 5 x per week – 15 piglets per filling; € 0,17 per piglet
Free-range poultry farm, using bottled gas
• 1 x per week; € 4,06 per week
• 5 x per week; € 13,00 per week

13. The Anoxia technique:
A good way to demonstrate your corporate responsibility
For more information: www.anoxia.eu