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The Procedure and
Classification of Column
Chromatography Process
• In the chemical industry, the process of purification plays
a very crucial role. The process mainly refers to
separating two different components for chemical use.
Separating two different components which are not
structurally similar is not a big deal.
Adsorbents for
Chromatography
Silica Gel
Chromatography
Thin Layer
Chromatography
Aluminium Oxide
Chromatography
Column
Chromatography
• But in cases where the structure and nature of the
components are similar, the purification process
needs some advanced technology. Column
chromatography is a technique that is used to
separate two almost similar nature components.
• One of the key
advantages of this
process is that it is
extremely cost effective.
The column, used in this
process is made of glass
with a diameter of 5 mm
to 50 mm. And the
height of the column is
somewhere between 5
cm to 1 meter. There is a
tap in the bottom as
well.
• Basically two methods
are followed to prepare
the column and those
are dry method and wet
method. In this process
the stationary phase is a
solid adsorbent mostly
silica gel or aluminium.
It is placed in the
column vertically. Then
there is the mobile
phase which is normally
liquid.
The Procedure and Classification of Column Chromatography Process

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The Procedure and Classification of Column Chromatography Process

  • 1. The Procedure and Classification of Column Chromatography Process
  • 2. • In the chemical industry, the process of purification plays a very crucial role. The process mainly refers to separating two different components for chemical use. Separating two different components which are not structurally similar is not a big deal.
  • 3. Adsorbents for Chromatography Silica Gel Chromatography Thin Layer Chromatography Aluminium Oxide Chromatography Column Chromatography
  • 4. • But in cases where the structure and nature of the components are similar, the purification process needs some advanced technology. Column chromatography is a technique that is used to separate two almost similar nature components.
  • 5. • One of the key advantages of this process is that it is extremely cost effective. The column, used in this process is made of glass with a diameter of 5 mm to 50 mm. And the height of the column is somewhere between 5 cm to 1 meter. There is a tap in the bottom as well.
  • 6. • Basically two methods are followed to prepare the column and those are dry method and wet method. In this process the stationary phase is a solid adsorbent mostly silica gel or aluminium. It is placed in the column vertically. Then there is the mobile phase which is normally liquid.