In terms of the nature of the solvent flow in the column, the process is categorized in two different types. When the flow of the solvent in the column is managed by gravity or by percolation, the process is called gravity column chromatography.
2. • In the chemical industry, the process of purification plays
a very crucial role. The process mainly refers to
separating two different components for chemical use.
Separating two different components which are not
structurally similar is not a big deal.
4. • But in cases where the structure and nature of the
components are similar, the purification process
needs some advanced technology. Column
chromatography is a technique that is used to
separate two almost similar nature components.
5. • One of the key
advantages of this
process is that it is
extremely cost effective.
The column, used in this
process is made of glass
with a diameter of 5 mm
to 50 mm. And the
height of the column is
somewhere between 5
cm to 1 meter. There is a
tap in the bottom as
well.
6. • Basically two methods
are followed to prepare
the column and those
are dry method and wet
method. In this process
the stationary phase is a
solid adsorbent mostly
silica gel or aluminium.
It is placed in the
column vertically. Then
there is the mobile
phase which is normally
liquid.