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PERSONAL PROTECTIVE
EQUIPMENT (PPE)
Personal Protective Equipment
(PPE)
“Specialized clothing or equipment

worn by an employee for
protection against infectious
materials”
How Can I Select PPE?
PPE selected for use should:
Protect HCP clothing and skin from
contamination by blood and body fluids.
 Protect patients from acquiring
microorganisms from HCP.


ICC
HEEPF ICEP
What are the types of PPE?
Gloves
 Gowns and aprons
 Mask
 Eye goggles
 Overhead
 Foot protection ???

Types of Gloves
Sterile Gloves
 Non-sterile Gloves.
 Heavy-duty Gloves.

Sterile Gloves



Disposable, individually wrapped.
They are mostly used during surgery
and during performing invasive
procedures.
Non- Sterile Gloves



Disposable, single use, usually not
singly wrapped.
They are mostly used to protect skin
against exposure to blood and body
fluids
(e.g. when changing dressing and
handling specimens).
Heavy – Duty Gloves
reusable after decontamination
Used for:
 Handling medical waste
 Performing environmental cleaning
Aprons and Gowns
Used during procedures that generate splashes
of blood or body fluids.

Apron

Gown
Eye Goggles
Used for protection against splashes of blood
or
body fluids (eg. during certain surgical
procedures).


Overhead
Used to contain hair during
surgical procedures
Masks
Placed over nose, mouth and
chin
Standard mask
Used when there is risk of
exposure to droplets
containing infectious agents
N95 masks
Used when there is risk of
exposure to airborne infectious
agents such as M. tuberculosis
(prevent >95% of particles less
Closed Foot Wear
In operating theatre

Over-shoes
“NOT RECOMMENDED”
Ideal way of transferring
micro-organisms from floor
and shoes to the hand.
What’s WRONG ?????
Safety in the laboratory
The laboratory safety procedures must
be read prior to attending the laboratory
session.
WHY??
Important in prevention of:

Infection

Injury by harmful chemicals

Mechanical
injury
by
glassware, open flames, and
sharp objects.


Personal belongings
away from
workstation


-

Wash your hands
with soap at the
start of the laboratory
session
before performing any
procedures
before leaving the
laboratory at the end
of the session.
Personal Protective Equipment (PPE) used:
o

Gloves are
essential.

o

Lab coats are
required.

o

Safety glasses
( goggles) may
be required to
avoid splashes.
Avoid touching objects (e.g., pencils, cell
phones, door handles) while wearing gloves.
Long hair must be tied back or covered to
minimize fire hazard or contamination of
experiments.
Laboratory personnel should not
wear sandals
Smoking in the lab is
essentially prohibited
Eating and drinking in the
laboratory are absolutely prohibited.






All cultures should be
handled as potentially
pathogenic.
Liquid cultures must
always be kept in a
test tube rack.
Broth cultures must
never be pipetted by
mouth.
In case of Blood Spills






Cover with paper
towel.
Apply disinfectant.
Leave for 15
minutes.
Remove while
wearing gloves.
Discard in a red
bag.
Report all accidents, no
matter how minor, to your
supervisor!!
BE CAREFUL
BE SAFE

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PPE & Safety in Lab - Prac. Microbiology