1. Polymerase Chain Reaction (PCR)

2. Nucleic acids - DNA

4. Nucleotides and nucleic acids Nucleotides are the building blocks of nucleic acids Nucleotide DNA RNA

24. Primers Target DNA 5’ 3’ 3’ 5’ forward reverse

32. The basic protocol—what’s in the tube Target DNA 5’ 3’ 3’ 5’ primers A B Free nucleotides Taq DNA polymerase Mg 2+ Mg 2+ Mg 2+ Mg 2+ Mg 2+ Mg 2+ Buffer containing magnesium

33. The basic protocol--denaturation Target DNA 95 o C 5’ 3’ 3’ 5’ 5’ 3’ 3’ 5’

34. The basic protocol--annealing ~55 o C 5’ 3’ 3’ 5’ 5’ 3’ 3’ 5’ 5’ 5’ Target DNA A B primers A B

35. The basic protocol--extension 72 o C 5’ 3’ 3’ 5’ 5’ 3’ 3’ 5’ 5’ 5’ Target DNA Taq polymerase

36. The basic protocol--extension 72 o C 5’ 3’ 3’ 5’ 5’ 3’ 3’ 5’ 5’ 5’ Target DNA

60. THANK YOU