1. K.KARTHIK,
5070,
VBM.

2. MASTITIS
• Inflammation of udder
Causative agents include
• Bacteria
• Fungi
• Algae,etc.,

3. INTRODUCTION
• Associated with over 26 species
• Candida spp., Aspergillus spp., Trichosporon
spp., Cryptococcus spp., Saccharomyces spp.,
Penicillium spp., etc. (CARTER, 1995).
• Fungi as the cause of mastitis -Rolle (1934),
isolated yeasts from five cows being fed on
brewery by-products.
• Candida is the most common species isolated
(Radostitis, 1995).

4. • Yeasts and Actinomycetes seem to be best
adapted to growth in the ducts and acini of the
udder
yeast yeast
actinomycetes

5. SYMPTOMS
• Gradual development of swelling (Pounden et
al .,1952)
• Loss of appetite and a rise in temperature but
absence of toxaemia (Simon et al.,1953)
• Swelling in all four quarters within 12 hours
(Galli ,1954)

6. MILK
• Greyish or yellowish floccules have been
reported – yeasts (Andersen & Jorgensen,
1949)
• A mucilaginous secretion - cryptococcal
infection

7. MASTITIS - GOAT
Markedly enlarged (about 2 times)
right (R) infected
Markedly swollen and enlarged
right udder half (R) goat 24 hrs
after infection with Candida
albicans.

8. HISTOPATHOLOGY
• Marked neutrophil infiltration
• Phagocytosed spores within the macrophages
• Extensive fibrosis in the interlobular septa
dividing the lobules

9. • Pseudohyphae and budding yeasts of C.
albicans mixed with purulent exudate in the
lumina of acini

10. Geotrichum candidum
• Keratinophilic yeast
• Extremely rare (mishra and panda, 1986;seddek, 1997;
krzyzanowski and sielicka, 1996).
• Any injury to teat sphinctures, or unhygienic milking,
can lead to its entry.
• Milk-watery with thick flakes, and slightly blood
tinged

11. • Microscopically the fungal mycelia were
septate and some were fragmented into
rectangular arthrospores
• Sensitive only to amphotericin-B.

12. DIAGNOSIS
• Strip cup test – change in colour,consistency
• Isolation and identification
aspergillus candida

13. cont
cryptococcus penicillum

14. CLINICAL IMPORTANCE
• Administration of antibiotics may aggravate
fungal mastitis as Candida spp. utilize
penicillin and tetracyclines as a source of
nitrogen (Meek, 1981).
candida
antibiotic

15. TREATMENT
Antifungal drugs like:-
• Nystatin , natamycin – for yeast mastitis
• Amphotericin B – geotrichum