2. • Ceftriaxone is a cephalosporin beta lactam antibiotic
• used in the treatment of bacterial infections caused by susceptible,
usually gram positive microorganisms.
Introduction
N
S
O
NH
O
N
O CH3
N
S
NH2
H H
O
O
Na
S
NH
N
N
CH3
O
OH
Na
+
OH2.7/2
3. • The mobile phase consisting of a binary mixture of acetonitrile and
buffer adjusted to pH 3.5 with orthophosphoric acid.
• Degassed by ultrasonic water bath and filtered.
Mobile phase and solutions
4. • Five different concentrations (10, 25, 50, 75 and 100 μg/ml) of
Ceftriaxone sodium solutions were prepared for linearity studies.
• The responses were measured as peak areas and plotted against
concentration.
Calibration
5. • The values of the relative
standard deviation of five
replicate injections of the
standard solutions lie well within
the limits.
Precision
6. • The values of the relative
standard deviation of
three replicate injections
of the standard solutions
lie well within the limits.
Accuracy
7. • The % Recovery for each level should be between 98.0 to 102.0%.
From the experimental value the recovery was found to be within
the limit (98.5%).
Acceptance Criteria for accuracy
8. • Correlation coefficient should be not less than 0.999. From the
experimental value the correlation coefficient was found to be the
same.
Linearity
9. • S/N Ratio value should be 3 for LOD. From the experimental value
the S/N ratio was found to be 2.87
Limit of detection
10. • S/N Ratio value should be 10 for LOQ solution. From the
experimental value the S/N ratio was found to be within limit i.e.
10.54
Limit of quantification
11. • When flow rate was varied at 0.7 ml/min to 0.9 ml/min it was found
that the variation in flow rate does not affect the method
significantly.
Robustness
