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Eriberto Bressan                                            Influence of abutment material on the
Gianluca Paniz
Diego Lops
                                                            gingival color of implant-supported
Boris Corazza                                               all-ceramic restorations: a prospective
Eugenio Romeo
Gianantonio Favero                                          multicenter study

Authors’ affiliations:                                       Key words: cad-cam technology, implant abutments, peri-implant soft tissue, spectrophot-
Eriberto Bressan, Department of Periodontology,             ometer
Dental Clinic, School of Dentistry, University of
Padova, Padova, Italy
Gianluca Paniz, Department of Prosthodontics and            Abstract
Operative Dentistry, Tufts University, Boston, MA,          Purpose: The aim of this clinical research on implant-supported restorations is to analyze, through
USA
Gianluca Paniz, Boris Corazza, Department of                spectrophotometric digital technology, the influence of the abutment material on the color of the
Prosthodontics, Dental Clinic, School of Dentistry,         peri-implant soft tissue.
University of Padova, Padova, Italy
                                                            Material and methods: Twenty patients received an endosseous dental implant in the anterior
Diego Lops, Department of Periodontology, Dental
Clinic, School of Dentistry, University of Milan, Milan,    maxilla. At the time of each definitive prosthesis delivery, an all-ceramic crown has been tried on gold,
Italy                                                       titanium and zirconia abutment. After the insertion of each single abutment, the peri-implant soft
Eugenio Romeo, Gianantonio Favero, Department of
                                                            tissue color has been measured through a spectrophotometer. Also, the thickness of the facial peri-
Prosthodontics, Dental Clinic, School of Dentistry,
University of Milan, Milan, Italy                           implant soft tissue was measured at the level of the implant neck through a caliper. A specific software
                                                            has been utilized to identify a specific tissue area and to collect the data before the statistical analysis
Corresponding author:
Gianluca Paniz
                                                            in Labn color space. The normality of the quantitative variables was verified by means of the Shapiro–
Department of Prosthodontics                                Wilk test. Simple linear correlation between quantitative variables was evaluated by using Pearson’s
Dental Clinic                                               coefficient. The results on the performance of the abutment materials with regard to the color
School of Dentistry
University of Padova                                        measurements and the overall measurement DE were described by computing the least-square means.
Via Cesarotti 31                                            The significance of differences among types of abutment was verified by means of the Scheffe test for
35100 Padova                                                multiple comparisons.
Italy
Tel.: þ 39 049 8751455                                      Results: For all the abutments used, the color of the peri-implant soft tissue appeared to be
Fax þ 39 049 8776791                                        significantly different from the one of the contra-lateral tooth (DE48.5). Significantly higher (Po0.05)
e-mail: panizg@hotmail.com                                  difference were present with the use of titanium abutments (11 Æ 0.4) when compared with the
                                                            results of gold (8.9 Æ 0.4) and zirconia (8.5 Æ 0.4) abutments. No correlation has been demonstrated
                                                            between soft tissue thickness and degree of color difference (P40.25).
                                                            Conclusions: Within the limitation of the present study, the peri-implant soft tissue color appears to
                                                            be different from the soft tissue color around natural teeth, no matter which type of restorative
                                                            material is selected. When titanium abutment was selected, significantly higher differences were
                                                            present than those obtained with gold or zirconia abutments. The thickness of the peri-implant soft
                                                            tissue did not appear to be a crucial factor in the abutment impact on the soft tissue color.


                                                            The preservation or reproduction of a natural          management are necessary for a natural outcome
                                                            mucogingival architecture surrounding dental           (Choquet et al. 2001; Kan et al. 2003; Grunder et
                                                            implants placed in the anterior maxilla is esthe-      al. 2005; Quirynen et al. 2007). Finally, the
                                                            tically challenging for the restorative dentist,       selection of the proper prosthetic solution, which
                                                            particularly when patients present with a high         is often overlooked, contributes significantly in
                                                            lip line when smiling. The challenge arises from       the achievement of a proper shade and shape of
                                                            the loss of muco-gingival tissue as a result of bone   the gingival tissue (Bichacho & Landsberg, 1997;
                                                            loss after extraction of traumatically injured or      Tarnow & Eskow 1996). The utilization of cus-
                                                            periodontally compromised teeth, or is due to a        tomized emergence profiles and abutments is
                                                            traumatic surgical extraction or congenital de-        critical for the achievement of proper esthetic
Date:                                                       fects (Buser et al. 2004).                             results (Gallucci et al. 2004).
Accepted 12 June 2010                                          The selection of a dental implant system that          Most recently, all-ceramic restorations have
To cite this article:                                       allows a proper biological response of the hard and    become increasingly popular for restoring teeth
Bressan E, Paniz G, Lops D, Corazza B, Romeo E, Favero G.   soft tissues, represents the first step for the         and implants. The advantages of all-ceramic re-
Influence Of abutment material on the gingival color of
implant supported all-ceramic restorations: a prospective   achievement of an adequate esthetic result (Sy-        storations cemented over metal abutments are
multicenter study                                           karas et al. 2000). Besides, a proper surgical         questionable, especially when the abutment
Clin. Oral Impl. Res. xx, 2010; 000–000.
doi: 10.1111/j.1600-0501.2010.02008.x                       technique, implant positioning and soft tissue         choice is for a highly translucent heat-pressed


 2010 John Wiley  Sons A/S
c                                                                                                                                                                    1
Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations


ceramic restoration (Nakamura et al. 2002). All-
ceramic abutments, made of aluminum oxide or
yttrium-stabilized zirconium oxide, have been
produced in an effort to overcome this esthetic
problem (Prestipino  Ingber 1993a; Wohlwend
et al. 1996; Heydeck et al. 2002; Rompen et al.
2007).
   The esthetic benefit of ceramic abutments over
metal abutments has been well documented in a
recent clinical study by (Jung et al. 2008). Any-
way, increased thicknesses of alumina and zirco-
nium oxide could compromise the esthetic result
due to an increased opacity and reduced translu-
cency (Hefferman et al. 2002a, 2002b); therefore,
the benefit might be controversial.
   The presence of different abutment closer to          Fig. 1. Thickness of the facial peri-implant soft tissue replica on the master cast (siliconic replica). Measurement performed at
the soft tissue might affect the esthetic appear-        the level of the implant neck through a caliper.
ance of the peri-implant soft tissue and alter its
color and appearance. In a recently published
study by (Jung et al. 2008), with the utilization
                                                         probing and plaque index inferior to 20%); (ii)                   of the provisional restoration in the definitive
of titanium or gold abutment with PFM Crown
                                                         no active intraoral or systemic disease; (iii) healed             cast (type IV dental stone, New Fuji-Rock, GC
or aluminum oxide abutment with all-ceramic
                                                         single edentulous site; and (iv) tooth contra-                    Corp., Tokyo, Japan). The thickness of the facial
crown, clinically noticeable differences were pre-
                                                         lateral to the placed implant: the natural tooth                  peri-implant soft tissue was measured at the level
sent in comparison with the contra-lateral tooth;
                                                         had to be present, vital and not restored.                        of the implant neck through a caliper (Iwanson
all-ceramic restorations revealed a significantly
                                                            Exclusion criteria were: (i) patients with sys-                Decimal Caliper, Asa Dental spa, Lucca, Italy)
better color match to the un-restored neighboring
                                                         temic diseases (such as heart, coagulation and                    (Figs. 1 and 2). The peri-implant mucosa was
teeth than porcelain-fused-to-metal restorations
                                                         leukocyte diseases or metabolic disorders); (ii)                  replied by using a light-consistance siliconic
on titanium or gold abutments.
                                                         history of radiation therapy in the head and                      material around the implant analog before the
   The purpose of this clinical trial on implant-
                                                         neck region; (iii) current treatment with steroids;               dental stone application so that an eventual
supported restorations is to analyze, through
                                                         (iv) neurological or psychiatric handicap that                    inaccuracy of the soft tissue reproduction steps
spectrophotometric digital technology, the influ-
                                                         could interfere with good oral hygiene; (v) im-                   could be reduced at least.
ence of the abutment material on the color of the
                                                         muno-compromised status, including infection                         The definitive prosthesis was an implant-sup-
peri-implant soft tissue. Moreover, the correla-
                                                         with human immunodeficiency virus; (vi) severe                     ported single crown cemented on a customized
tion between soft tissue thickness and digital
                                                         clenching or bruxism; (vii) smoking habit                         abutment. One full coverage restoration was
evaluation will be analyzed in order to verify
                                                         (415 cigarettes/die); (viii) drug or alcohol abuse;               fabricated for each single implant with the utili-
possible esthetic implications for the utilization
                                                         and (ix) inadequate compliance.                                   zation of a zirconia coping (Lava, 3M ESPE,
of different types of abutment material related to
                                                            A two-stage surgical technique and no addi-                    Seefeld, Germany) and feldsphatic porcelain stra-
different clinical situations.
                                                         tional soft or hard tissue graft were planned for all             tification (Ziro X veneering ceramic, Wieland
                                                         the implants. All implants were submerged and                     Dental þ Technik GmbH  Co. KG, Pforzheim,
                                                         all parts of the defects were covered by mucosal                  Germany).
Material and methods                                     tissue. Removable prostheses or provisional fixed                     The customized abutment was the variable of
                                                         bridges were adjusted.                                            the present study and three different materials
Twenty patients have been included in this pro-             Four months after implant placement, surgical                  have been utilized: titanium, gold-alloy and zir-
spective multicenter study. All patients have            re-entry occurred; implant stage 2 was performed                  conia as shown in Table 1. The facial and inter-
been treated at the University of Padova Dental          by the same operator and a trans-mucosal healing                  proximal margin level were kept 1mm sub-
School and at Dental Clinic of Biomedical                abutment (Healing Abutment 3.5/4.0, Astra                         gingival while the palatal margin was left equi-
Sciences Institute, St. Paul Hospital, University        Tech Dental Implant) was inserted [Time 0].                       gingival. CAD-CAM technology (WizBlade,
of Milan, Italy. The study protocol was approved            Two weeks after surgical re-entry, an implant-                 Nextec Technologies 2001 Ltd., Tirat HaCar-
by the University of Padova and Milan Institu-           level impression was taken for the fabrication of a               mel, Israel) was used to create the same morphol-
tional Ethics Committees. Informed consent was           screw-retained temporary restoration [Time 1].                    ogy for all the 3 types of abutments using a
obtained from all subjects.                              The provisional restoration was inserted 1 week                   milling machine (MIKRON HSM 400U
   Each single patient received an endosseous            after implant level impression [Time 2]. After 8                  ProdMed, Angie Charmilles International SA,
dental implant (Osseospeed 4.0s, Astra Tech              weeks of soft tissue conditioning by means of the                 Geneva, Switzerland).
Dental Implant, Astra Tech AB, Molndal, Swe-             provisional restoration, a definitive implant level                   Fourteen weeks after implant stage 2 [Time 4],
den), which has been placed in the anterior              impression was taken; a precise record of the soft                the temporary restoration was removed and defi-
maxilla (area from tooth 1.5 and 2.5).                   tissue dimensions [Time 3] was recorded. The                      nitive customized abutments were screwed
   To be included in the study, all patients pre-        pick-up impression coping was modified by add-                     (Ratchet Wrench, Astra Dental Implant). The
sented: (i) controlled periodontal condition (no         ing a self-polymerizing resin (Duralay, Chicago,                  definitive all-ceramic crown (Lava, 3M ESPE)
PPD index superior to 4 mm, no bleeding on               IL, USA) in order to reply the emergence profile                   was temporarily placed on the abutment with a


2 |   Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x                                                                                         2010 John Wiley  Sons A/S
                                                                                                                                                    c
Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations


                                                                                                                           nologies, Arbizzano di Negrar, Verona, Italy).
                                                                                                                           The device was managed by a single operator
                                                                                                                           who captured an area of about 5 mm around the
                                                                                                                           gingival margin of the selected tooth or crown.
                                                                                                                           Each selected area was measured for three times.
                                                                                                                           After all the measurements, an evaluation of the
                                                                                                                           esthetic outcome was also performed by the
                                                                                                                           operator and by the patient and the best solution
                                                                                                                           was delivered to the patient.
                                                                                                                              The selected abutment was torqued down to
                                                                                                                           25 N/cm (Abutment Screw, Astra Tech Dental
                                                                                                                           Implant) with a torque wrench (Torque Wrench,
                                                                                                                           Astra Tech Dental Implant) and the definitive
                                                                                                                           all-ceramic crown was cemented with a tempor-
                                                                                                                           ary cement (Temp-Bond Clear, Kerr Corporation,
                                                                                                                           Orange, CA, USA).
Fig. 2. Correspondent occlusal view of the dental implant and the facial peri-implant soft tissue.                            All the measured areas were analyzed through
                                                                                                                           the spectrophotometer software (Spectroshade
                                                                                                                           3.01, MHT S.p.A.) which identified a specific
Table 1. Abutment type
                                                                                                                           area. The selected area extended from the gingi-
   Abutment       Abutment material
                                                                                                                           val level to 4 mm sub-gingival and from the long
   type
                                                                                                                           axis of the tooth 2 mm on each side as shown in
   Type 1         Cast-to abutments 3.5/4.0 (Astra Tech Dental Implant) Keramit Eco LF, Micro Fine Grain
                                                                                                                           Fig. 6. The results of each measured area
                  Alloy (Au 57%, Pd 10,6%, Ag 29,2%) (NobilMetal S.p.A., Villafranca d’Asti, Italy)
   Type 2         Titanium abutment: platform 3.5/4.0 (Astra Tech Dental Implant)                                          were recorded through Labn color scale and the
   Type 3         Zirconia abutment: platform 3.5/4.0 (Astra Tech Dental Implant)                                          values from the three measurements were
                                                                                                                           averaged before proceeding with the statistical
                                                                                                                           analysis. The comparison between the peri-im-
                                                                                                                           plant soft tissue and the contra-lateral gingival
                                                                                                                           tissue was performed with the use of the follow-
                                                                                                                           ing DE formula:DE ¼ (DL)2 þ (Da)2 þ (Db)2. A cri-
                                                                                                                           tical threshold of DE 3.7 for intraoral color
                                                                                                                           distinction by the naked eye was considered
                                                                                                                           (Munsell, 1923; Hunt 1987; Johnston  Kao
                                                                                                                           1989; Berns 2000).
                                                                                                                              Statistical analysis was performed to investi-
                                                                                                                           gate the performance of the abutment materials.
                                                                                                                           The normality of the quantitative variables was
                                                                                                                           verified by means of the Shapiro-Wilk test.
                                                                                                                           Simple linear correlation between quantitative
                                                                                                                           variables was evaluated by using Pearson’s coef-
                                                                                                                           ficient. The results on the performance of the
                                                                                                                           abutment materials with regard to the color
                                                                                                                           measurements and the overall measurement dE
Fig. 3. Titanuim abutment screwed into position.                                                                           were described by computing the least-square
                                                                                                                           means as well as their standard errors (SE). To
try-in paste (Variolink trial base, Ivoclar Viva-               between the prosthesis insertion and the color             obtain the adjusted least-square means, general
dent, Ivoclar Vivadent AG, Schaan, Liechten-                    measurement in order to have a stable tissue color.        linear models were applied with types of abut-
stein).                                                         After the completion of the color measurement of           ment and patients as main fixed effects.
   Each type of abutment (Table 1) was left in the              the second abutment, the peri-implant soft tissue            This method also provided the 95% confi-
mouth for 10 min, with the correspondent crown                  around the third one was measured in the same              dence intervals (95% CI) for the mean values.
positioned, before proceeding with the color mea-               manner (Figs. 3–5). The sequence of choice of the          The significance of differences among types of
surement of the peri-implant soft tissue. Imme-                 type of abutment was randomly selected on each             abutment was verified by means of the Scheffe
diately after the first color measurement, the all-              single case. The measurement of the contra-lateral         test for multiple comparisons, or by the Dunnett
ceramic restoration was removed, the first abut-                 soft tissue area (control site), adjacent to a natural     test when the not-treated teeth (control sites)
ment was unscrewed and the second one was                       tooth, was also performed in order to obtain a             characteristics were used as reference.
positioned in the same manner of the first one.                  standard reference measurement.                              The significance level was fixed at 0.05, and all
Even if without altering the shape of the defini-                   The color measurement has been obtained                 tests were two tailed. All the analyses were
tive prosthesis from the final one, no pressure on               using a spectrophotometer (Spectroshade ‘‘Mi-              performed using the SAS statistical software rel.
the soft tissue was noticed, 10 min were left                   cro’’ Device, MHT S.p.A., Medical High Tech-               9.1 (SAS Institute, Cary, NC, USA).


 2010 John Wiley  Sons A/S
c                                                                                                                3 |     Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x
Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations


                                                                                                                              Table 2. Estimated least square mean Æ stan-
                                                                                                                              dard errors (SE) and 95% confidence intervals
                                                                                                                              (95% CI) of DE by abutment materials
                                                                                                                                                   Mean Æ SE             95% CI
                                                                                                                                Gold               8.9a Æ 0.4             8.1–9.7
                                                                                                                                Titanium           11b Æ 0.4             10.2–11.9
                                                                                                                                Zirconium          8.5a Æ 0.4             7.6–9.3

                                                                                                                                Different superscript letters indicate significantly
                                                                                                                                (Po0.05) different mean values (Scheffe’s test for
                                                                                                                                multiple comparison).


                                                                                                                              naked eye. When the crown was placed over the
                                                                                                                              abutment, the DE mean value between the peri-
                                                                                                                              implant soft tissue and the contra-lateral gingival
                                                                                                                              tissue was similar for gold and zirconium abut-
                                                                                                                              ment (8.9 and 8.5, respectively), but it was sig-
                                                                                                                              nificantly greater with the titanium abutment (11).
Fig. 4. Gold-alloy abutment screwed into position.                                                                               The calculated Lab (L, a, b) values of the
                                                                                                                              measured areas around abutments and natural
                                                                                                                              teeth were summarized on Table 3. For each
                                                                                                                              feature, natural teeth mean values were signifi-
                                                                                                                              cantly higher than those of the different types of
                                                                                                                              abutment. In addition, gold and titanium abut-
                                                                                                                              ments provided no significantly different mean
                                                                                                                              values both for L and b color measurement, while
                                                                                                                              titanium abutment always provided lower mean
                                                                                                                              values. For the a color measurements, no differ-
                                                                                                                              ences were evident among the three types of
                                                                                                                              abutment. Gold, titanium and zirconia abutment
                                                                                                                              measurements were significantly different
                                                                                                                              (Po0.001) from contra-lateral teeth (control
                                                                                                                              sites) measurements.
                                                                                                                                 Calculated Lab values of different abutments
                                                                                                                              with correspondent 95% CI are reported in Fig. 7;
                                                                                                                              control sites were considered as reference. All the
Fig. 5. Zirconia abutment screwed into position.                                                                              differences evaluated by using paired data were
                                                                                                                              statistically significant, as shown by 95% CIs
                                                                                                                              (zero value not included). Fig. 7 also showed that
                                                                                                                              titanium abutment exhibited worse color perfor-
                                                                                                                              mances than gold and zirconium abutments. The
                                                                                                                              zirconia results were likely to achieve the best
                                                                                                                              color matching with natural teeth, but the differ-
                                                                                                                              ences with gold performance were never statisti-
                                                                                                                              cally significant.
                                                                                                                                 To verify the role of soft tissue thickness on the
                                                                                                                              color measurements with the different abutment
                                                                                                                              types, a stratified analysis was performed by
                                                                                                                              dichotomizing the thickness on 2 mm (thin
                                                                                                                                  2 mm; thick 42 mm). Seven patients were
                                                                                                                              included in the ‘‘Thin’’ group and the other 13
                                                                                                                              patients made up the ‘‘Thick’’ group, respec-
                                                                                                                              tively. The DE mean value did not depend on
                                                                                                                              the soft tissue thickness (Table 4), and thickness
                                                                                                                                                         ¨
                                                                                                                              might only relate to the Ab color aspect (Table 5).
Fig. 6. Selected area identified through spectrophotometer software: 2 mm in thickness (red arrow) and 4 mm in apico-coronal
direction (green arrow).


Results                                                        matter which type of restorative material was                  Discussion
                                                               selected (Table 2). Measurements relative to all
The peri-implant soft tissue color was different               the abutments were above the critical threshold                The present study evaluated the color-change
from the soft tissue color around natural teeth, no            of DE 3.7 for intraoral color distinction by the               effect on marginal peri-implant soft tissue of all-


4 |   Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x                                                                                         2010 John Wiley  Sons A/S
                                                                                                                                                    c
Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations


Table 3. Estimated least square mean Æ standard errors (SE) of Lab values: measured areas around                        Table 5. Correlation coefficients between soft
abutments and natural teeth by abutment materials                                                                       tissue thickness (mm) and the difference for the
                                                                                                                        Lab values of the measured areas around abut-
                  Gold                         Titanium                   Zirconium                NT                   ments and natural teeth or DE, by abutment
                  Mean Æ SE                    Mean Æ SE                  Mean Æ SE                Mean Æ SE            materials
                                                                                                                               Gold             Titanium        Zirconium
  Ln              47.1a,b Æ 0.6                45.6a Æ 0.6                48.1b Æ 0.6              52.9c Æ 0.6
  an               22.1a Æ 0.5                 21.5a Æ 0.5                22.9a Æ 0.5              25.5b Æ 0.5             DL    0.2   (0.38)   À 0.06   (0.81) À 0.04 (0.87)
  bn              14.9a,b Æ 0.3                13.9a Æ 0.3                15.4b Æ 0.3              18.4c Æ 0.3             DA À 0.3    (0.19)   À 0.26   (0.26) À 0.2 (0.38)
                                                                                                                           DB À 0.45   (0.05)    À 0.4   (0.08) À 0.4 (0.08)
  Different superscript letters indicate significantly different mean values.                                               DE   0.08   (0.74)     0.09   (0.71)   0.13 (0.6)

                                                                                                                           Significance in brackets.



                                                                                                                        study showed a significant difference between
                                                                                                                        the color of the peri-implant soft tissue and the
                                                                                                                        color of the gingival around natural teeth no
                                                                                                                        matter which type of prosthesis is used. As
                                                                                                                        described on Table 2, DEs were always higher
                                                                                                                        than the critical threshold of 3.7 (Johnston  Kao
                                                                                                                        1989). This value, described as higher value for
                                                                                                                        color matching between natural teeth and re-
                                                                                                                        stored teeth, might have only a relative impact
                                                                                                                        on the analysis of the soft tissue but represent the
                                                                                                                        most reported reference in the literature (Ishi-
                                                                                                                        kawa et al. 1988; Jung et al. 2008; Lops et al.
                                                                                                                        2008; Romeo et al. 2008). Other values reported
                                                                                                                        in the tooth color matching literature are even
                                                                                                                        lower (Douglas, 1997; Douglas  Brewer, 1998).
                                                                                                                        The absence in the literature of a reference for
                                                                                                                        soft tissue color matching may represented a
                                                                                                                        limitation in the analysis of present results, but
                                                                                                                        the DE values obtained are significantly higher
                                                                                                                        than the reported color differences between the
                                                                                                                        soft tissues of contra-lateral natural teeth, which
                                                                                                                        were within 2.7 (Zembic et al. 2009).
                                                                                                                           As described on Table 3, significantly different
                                                                                                                        mean values have been found between titanium
                                                                                                                        on one side and gold and zirconia on the other
                                                                                                                        side. While no differences were present in the
                                                                                                                        color performance of gold and zirconium oxide
                                                                                                                        abtments, the color of the soft tissue around
                                                                                                                        titanium abutment was significantly more differ-
                                                                                                                        ent from the gingival color around natural teeth.
Fig. 7. Calculated Lab values with correspondent 95% confidence interval represented by type of abutment: taking not
                                                                                                                           In addition, all the single values (Ln, an, bn)
treated teeth (control site) taken as reference.
                                                                                                                        were different from the values obtained around
                                                                                                                        natural teeth. Among the different type of abut-
                                                                                                                        ments, no significant differences were present in
Table 4. DE mean values and standard errors (SE) by soft tissue thickness and abutment materials
                                                                                                                        considering the red and green scale of a values,
                                Soft tissue thickness (mean Æ SE)                                          P
                                                                                                                        confirming a trend already reported in the litera-
                                   2 mm (7 pt)                         42mm (13 pt)                                     ture (Hermann et al. 2001). In the analysis of Ln
  Gold                          8.6 Æ 1.4                               9.1 Æ 0.8                          0.74         and bn values, significantly differences were pre-
  Titanium                      9.5 Æ 1.4                              11.9 Æ 1.2                          0.25         sent among the abutment. The peri-implant soft
  Zirconium                     7.5 Æ 1.4                               8.9 Æ 0.7                          0.38
                                                                                                                        tissue around zirconium oxide abutments ap-
                                                                                                                        peared to be significantly closer to the color of
                                                                                                                        natural teeth gingival than titanium and gold
ceramic restorations based on zirconium oxide,               over, different from previous studies, only one            abutment provided interposed results. Also,
gold-alloy and titanium abutments. The pecu-                 type of crown was utilized, relating the results           (Jung et al. 2008) noticed clinically gingival color
liarity of this trial was that measurements were             only to the abutment variable.                             differences between around peri-implant soft tis-
performed for all three abutment materials in                  In accordance with the data available in the             sue in comparison with the contra-lateral tooth.
each patient; therefore, important patient-related           international literature by (Sailer et al. 2009) and       Nevertheless, their results suggested that all-
and material-related biases were avoided. More-              (Zembic et al. 2009), the results of the present           ceramic restorations on aluminum oxide abut-


 2010 John Wiley  Sons A/S
c                                                                                                              5 |    Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x
Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations


ments provide a significantly better color match            at 2 mm: 7 patients with thin and 13 with thick          esting to compare the accuracy of this method
to the un-restored neighboring teeth than porce-           peri-implant soft-tissues were observed. As              with that used by (Sailer et al. 2009) and (Zembic
lain-fused-to-metal restorations on titanium or            shown on Table 4, no statistically significant            et al. 2009). They recorded the soft tissue thick-
gold abutments. Unfortunately, no specification             differences were present in the color of the             ness in the region of esthetic assessment both at
on the number of gold or titanium abutment nor             mucosa between thick and thin tissue. Even if a          implants and teeth using an endodontic file (ISO
a description on the possible different outcome            trend of higher values of differences was noticed,       #20) with a rubber stop.
present between these two types of abutment has            especially with titanium abutment, in the thick
been made. Moreover, considering the properties            group, this study did not confirm that mucosa
of zirconium oxide vs. aluminum oxide, zirco-              thickness mucosa is a crucial factor in terms of
nium oxide should be referred on implant-                  discoloration as concluded by (Jung et al. 2007).
supported restorations (Butz et al. 2005; Canullo,         Most probably this result differed from the pre-         Conclusions
2007; Garine et al. 2007).                                 vious literature due to the reduced sample size.
   Another in vitro study by (Jung et al. 2007)            Another reason for this trend could be that no soft      Within the limitation of the resent study, the
reported a decrease of the color change related to         tissue thickness ! 3 mm was found in the                 following conclusions can be drawn:
an increasing of the mucosa thickness over zirco-          group classified as ‘‘Thick.’’ Further researches
nia and titanium. In situations with a mucosal             on more representative patient samples are re-            the peri-implant soft tissue color is different
thickness of 1.5 mm, both materials demon-                 quested to confirm or refuse this trend.                    from the soft tissue color around natural
strated DE values above the critical threshold of             Regarding an eventual shortcoming of the thick-         teeth, no matter which type of restorative
3.7 (Park et al. 2007), with a score of 3.87 for           ness measurement by means of a caliper applied to          material is selected.
zirconium oxide and 5.06 for titanium. With a              the study cast), the authors considered that a            titanium abutments are associated with sig-
mucosa thickness of 2 mm, the color change                 customized pick-up was able to permit a precise            nificantly higher differences than those ob-
induced by zirconia was below the threshold of             reproduction of the trans-mucosal soft tissue cone,        tained with gold or zirconia abutments.
3.7 (3.17), whereas titanium still caused a visible        and a light-consistence polyether material could          no significant differences are present between
difference (4.32). With a 3 mm thickness, all the          not interfere in the soft tissue thickness re-produc-      the color of peri-implant soft tissue around
abutment scored within the limit of 3.7 (titanium          tion. Probably, the subsequent step of the gingival        zirconia abutment and the one around gold
2.14 and zirconia 2.47).                                   re-construction on the master cast by means of a           abutment.
   For this reason, the present study also tried to        siliconic material could add some degree of inac-         the thickness of the peri-implant soft tissue
assess possible in vivo implication of the thick-          curacy (maybe fraction of millimeter) in the peri-         does not appear to be a crucial factor in the
ness of peri-implant mucosa by dichotomizing it            implant mucosa re-production. It could be inter-           abutment impact on the soft tissue color.



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  plants 22: 203–223.                                        Zirkonoxidabutment-Ein neues vollkera-misches                 to the corresponding author for the article.




 2010 John Wiley  Sons A/S
c                                                                                                               7 |      Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x

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Influence of abutment material on the gingival color of implant-supported all-ceramic restorations: a prospective multicenter study

  • 1. Eriberto Bressan Influence of abutment material on the Gianluca Paniz Diego Lops gingival color of implant-supported Boris Corazza all-ceramic restorations: a prospective Eugenio Romeo Gianantonio Favero multicenter study Authors’ affiliations: Key words: cad-cam technology, implant abutments, peri-implant soft tissue, spectrophot- Eriberto Bressan, Department of Periodontology, ometer Dental Clinic, School of Dentistry, University of Padova, Padova, Italy Gianluca Paniz, Department of Prosthodontics and Abstract Operative Dentistry, Tufts University, Boston, MA, Purpose: The aim of this clinical research on implant-supported restorations is to analyze, through USA Gianluca Paniz, Boris Corazza, Department of spectrophotometric digital technology, the influence of the abutment material on the color of the Prosthodontics, Dental Clinic, School of Dentistry, peri-implant soft tissue. University of Padova, Padova, Italy Material and methods: Twenty patients received an endosseous dental implant in the anterior Diego Lops, Department of Periodontology, Dental Clinic, School of Dentistry, University of Milan, Milan, maxilla. At the time of each definitive prosthesis delivery, an all-ceramic crown has been tried on gold, Italy titanium and zirconia abutment. After the insertion of each single abutment, the peri-implant soft Eugenio Romeo, Gianantonio Favero, Department of tissue color has been measured through a spectrophotometer. Also, the thickness of the facial peri- Prosthodontics, Dental Clinic, School of Dentistry, University of Milan, Milan, Italy implant soft tissue was measured at the level of the implant neck through a caliper. A specific software has been utilized to identify a specific tissue area and to collect the data before the statistical analysis Corresponding author: Gianluca Paniz in Labn color space. The normality of the quantitative variables was verified by means of the Shapiro– Department of Prosthodontics Wilk test. Simple linear correlation between quantitative variables was evaluated by using Pearson’s Dental Clinic coefficient. The results on the performance of the abutment materials with regard to the color School of Dentistry University of Padova measurements and the overall measurement DE were described by computing the least-square means. Via Cesarotti 31 The significance of differences among types of abutment was verified by means of the Scheffe test for 35100 Padova multiple comparisons. Italy Tel.: þ 39 049 8751455 Results: For all the abutments used, the color of the peri-implant soft tissue appeared to be Fax þ 39 049 8776791 significantly different from the one of the contra-lateral tooth (DE48.5). Significantly higher (Po0.05) e-mail: panizg@hotmail.com difference were present with the use of titanium abutments (11 Æ 0.4) when compared with the results of gold (8.9 Æ 0.4) and zirconia (8.5 Æ 0.4) abutments. No correlation has been demonstrated between soft tissue thickness and degree of color difference (P40.25). Conclusions: Within the limitation of the present study, the peri-implant soft tissue color appears to be different from the soft tissue color around natural teeth, no matter which type of restorative material is selected. When titanium abutment was selected, significantly higher differences were present than those obtained with gold or zirconia abutments. The thickness of the peri-implant soft tissue did not appear to be a crucial factor in the abutment impact on the soft tissue color. The preservation or reproduction of a natural management are necessary for a natural outcome mucogingival architecture surrounding dental (Choquet et al. 2001; Kan et al. 2003; Grunder et implants placed in the anterior maxilla is esthe- al. 2005; Quirynen et al. 2007). Finally, the tically challenging for the restorative dentist, selection of the proper prosthetic solution, which particularly when patients present with a high is often overlooked, contributes significantly in lip line when smiling. The challenge arises from the achievement of a proper shade and shape of the loss of muco-gingival tissue as a result of bone the gingival tissue (Bichacho & Landsberg, 1997; loss after extraction of traumatically injured or Tarnow & Eskow 1996). The utilization of cus- periodontally compromised teeth, or is due to a tomized emergence profiles and abutments is traumatic surgical extraction or congenital de- critical for the achievement of proper esthetic Date: fects (Buser et al. 2004). results (Gallucci et al. 2004). Accepted 12 June 2010 The selection of a dental implant system that Most recently, all-ceramic restorations have To cite this article: allows a proper biological response of the hard and become increasingly popular for restoring teeth Bressan E, Paniz G, Lops D, Corazza B, Romeo E, Favero G. soft tissues, represents the first step for the and implants. The advantages of all-ceramic re- Influence Of abutment material on the gingival color of implant supported all-ceramic restorations: a prospective achievement of an adequate esthetic result (Sy- storations cemented over metal abutments are multicenter study karas et al. 2000). Besides, a proper surgical questionable, especially when the abutment Clin. Oral Impl. Res. xx, 2010; 000–000. doi: 10.1111/j.1600-0501.2010.02008.x technique, implant positioning and soft tissue choice is for a highly translucent heat-pressed 2010 John Wiley Sons A/S c 1
  • 2. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations ceramic restoration (Nakamura et al. 2002). All- ceramic abutments, made of aluminum oxide or yttrium-stabilized zirconium oxide, have been produced in an effort to overcome this esthetic problem (Prestipino Ingber 1993a; Wohlwend et al. 1996; Heydeck et al. 2002; Rompen et al. 2007). The esthetic benefit of ceramic abutments over metal abutments has been well documented in a recent clinical study by (Jung et al. 2008). Any- way, increased thicknesses of alumina and zirco- nium oxide could compromise the esthetic result due to an increased opacity and reduced translu- cency (Hefferman et al. 2002a, 2002b); therefore, the benefit might be controversial. The presence of different abutment closer to Fig. 1. Thickness of the facial peri-implant soft tissue replica on the master cast (siliconic replica). Measurement performed at the soft tissue might affect the esthetic appear- the level of the implant neck through a caliper. ance of the peri-implant soft tissue and alter its color and appearance. In a recently published study by (Jung et al. 2008), with the utilization probing and plaque index inferior to 20%); (ii) of the provisional restoration in the definitive of titanium or gold abutment with PFM Crown no active intraoral or systemic disease; (iii) healed cast (type IV dental stone, New Fuji-Rock, GC or aluminum oxide abutment with all-ceramic single edentulous site; and (iv) tooth contra- Corp., Tokyo, Japan). The thickness of the facial crown, clinically noticeable differences were pre- lateral to the placed implant: the natural tooth peri-implant soft tissue was measured at the level sent in comparison with the contra-lateral tooth; had to be present, vital and not restored. of the implant neck through a caliper (Iwanson all-ceramic restorations revealed a significantly Exclusion criteria were: (i) patients with sys- Decimal Caliper, Asa Dental spa, Lucca, Italy) better color match to the un-restored neighboring temic diseases (such as heart, coagulation and (Figs. 1 and 2). The peri-implant mucosa was teeth than porcelain-fused-to-metal restorations leukocyte diseases or metabolic disorders); (ii) replied by using a light-consistance siliconic on titanium or gold abutments. history of radiation therapy in the head and material around the implant analog before the The purpose of this clinical trial on implant- neck region; (iii) current treatment with steroids; dental stone application so that an eventual supported restorations is to analyze, through (iv) neurological or psychiatric handicap that inaccuracy of the soft tissue reproduction steps spectrophotometric digital technology, the influ- could interfere with good oral hygiene; (v) im- could be reduced at least. ence of the abutment material on the color of the muno-compromised status, including infection The definitive prosthesis was an implant-sup- peri-implant soft tissue. Moreover, the correla- with human immunodeficiency virus; (vi) severe ported single crown cemented on a customized tion between soft tissue thickness and digital clenching or bruxism; (vii) smoking habit abutment. One full coverage restoration was evaluation will be analyzed in order to verify (415 cigarettes/die); (viii) drug or alcohol abuse; fabricated for each single implant with the utili- possible esthetic implications for the utilization and (ix) inadequate compliance. zation of a zirconia coping (Lava, 3M ESPE, of different types of abutment material related to A two-stage surgical technique and no addi- Seefeld, Germany) and feldsphatic porcelain stra- different clinical situations. tional soft or hard tissue graft were planned for all tification (Ziro X veneering ceramic, Wieland the implants. All implants were submerged and Dental þ Technik GmbH Co. KG, Pforzheim, all parts of the defects were covered by mucosal Germany). Material and methods tissue. Removable prostheses or provisional fixed The customized abutment was the variable of bridges were adjusted. the present study and three different materials Twenty patients have been included in this pro- Four months after implant placement, surgical have been utilized: titanium, gold-alloy and zir- spective multicenter study. All patients have re-entry occurred; implant stage 2 was performed conia as shown in Table 1. The facial and inter- been treated at the University of Padova Dental by the same operator and a trans-mucosal healing proximal margin level were kept 1mm sub- School and at Dental Clinic of Biomedical abutment (Healing Abutment 3.5/4.0, Astra gingival while the palatal margin was left equi- Sciences Institute, St. Paul Hospital, University Tech Dental Implant) was inserted [Time 0]. gingival. CAD-CAM technology (WizBlade, of Milan, Italy. The study protocol was approved Two weeks after surgical re-entry, an implant- Nextec Technologies 2001 Ltd., Tirat HaCar- by the University of Padova and Milan Institu- level impression was taken for the fabrication of a mel, Israel) was used to create the same morphol- tional Ethics Committees. Informed consent was screw-retained temporary restoration [Time 1]. ogy for all the 3 types of abutments using a obtained from all subjects. The provisional restoration was inserted 1 week milling machine (MIKRON HSM 400U Each single patient received an endosseous after implant level impression [Time 2]. After 8 ProdMed, Angie Charmilles International SA, dental implant (Osseospeed 4.0s, Astra Tech weeks of soft tissue conditioning by means of the Geneva, Switzerland). Dental Implant, Astra Tech AB, Molndal, Swe- provisional restoration, a definitive implant level Fourteen weeks after implant stage 2 [Time 4], den), which has been placed in the anterior impression was taken; a precise record of the soft the temporary restoration was removed and defi- maxilla (area from tooth 1.5 and 2.5). tissue dimensions [Time 3] was recorded. The nitive customized abutments were screwed To be included in the study, all patients pre- pick-up impression coping was modified by add- (Ratchet Wrench, Astra Dental Implant). The sented: (i) controlled periodontal condition (no ing a self-polymerizing resin (Duralay, Chicago, definitive all-ceramic crown (Lava, 3M ESPE) PPD index superior to 4 mm, no bleeding on IL, USA) in order to reply the emergence profile was temporarily placed on the abutment with a 2 | Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x 2010 John Wiley Sons A/S c
  • 3. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations nologies, Arbizzano di Negrar, Verona, Italy). The device was managed by a single operator who captured an area of about 5 mm around the gingival margin of the selected tooth or crown. Each selected area was measured for three times. After all the measurements, an evaluation of the esthetic outcome was also performed by the operator and by the patient and the best solution was delivered to the patient. The selected abutment was torqued down to 25 N/cm (Abutment Screw, Astra Tech Dental Implant) with a torque wrench (Torque Wrench, Astra Tech Dental Implant) and the definitive all-ceramic crown was cemented with a tempor- ary cement (Temp-Bond Clear, Kerr Corporation, Orange, CA, USA). Fig. 2. Correspondent occlusal view of the dental implant and the facial peri-implant soft tissue. All the measured areas were analyzed through the spectrophotometer software (Spectroshade 3.01, MHT S.p.A.) which identified a specific Table 1. Abutment type area. The selected area extended from the gingi- Abutment Abutment material val level to 4 mm sub-gingival and from the long type axis of the tooth 2 mm on each side as shown in Type 1 Cast-to abutments 3.5/4.0 (Astra Tech Dental Implant) Keramit Eco LF, Micro Fine Grain Fig. 6. The results of each measured area Alloy (Au 57%, Pd 10,6%, Ag 29,2%) (NobilMetal S.p.A., Villafranca d’Asti, Italy) Type 2 Titanium abutment: platform 3.5/4.0 (Astra Tech Dental Implant) were recorded through Labn color scale and the Type 3 Zirconia abutment: platform 3.5/4.0 (Astra Tech Dental Implant) values from the three measurements were averaged before proceeding with the statistical analysis. The comparison between the peri-im- plant soft tissue and the contra-lateral gingival tissue was performed with the use of the follow- ing DE formula:DE ¼ (DL)2 þ (Da)2 þ (Db)2. A cri- tical threshold of DE 3.7 for intraoral color distinction by the naked eye was considered (Munsell, 1923; Hunt 1987; Johnston Kao 1989; Berns 2000). Statistical analysis was performed to investi- gate the performance of the abutment materials. The normality of the quantitative variables was verified by means of the Shapiro-Wilk test. Simple linear correlation between quantitative variables was evaluated by using Pearson’s coef- ficient. The results on the performance of the abutment materials with regard to the color measurements and the overall measurement dE Fig. 3. Titanuim abutment screwed into position. were described by computing the least-square means as well as their standard errors (SE). To try-in paste (Variolink trial base, Ivoclar Viva- between the prosthesis insertion and the color obtain the adjusted least-square means, general dent, Ivoclar Vivadent AG, Schaan, Liechten- measurement in order to have a stable tissue color. linear models were applied with types of abut- stein). After the completion of the color measurement of ment and patients as main fixed effects. Each type of abutment (Table 1) was left in the the second abutment, the peri-implant soft tissue This method also provided the 95% confi- mouth for 10 min, with the correspondent crown around the third one was measured in the same dence intervals (95% CI) for the mean values. positioned, before proceeding with the color mea- manner (Figs. 3–5). The sequence of choice of the The significance of differences among types of surement of the peri-implant soft tissue. Imme- type of abutment was randomly selected on each abutment was verified by means of the Scheffe diately after the first color measurement, the all- single case. The measurement of the contra-lateral test for multiple comparisons, or by the Dunnett ceramic restoration was removed, the first abut- soft tissue area (control site), adjacent to a natural test when the not-treated teeth (control sites) ment was unscrewed and the second one was tooth, was also performed in order to obtain a characteristics were used as reference. positioned in the same manner of the first one. standard reference measurement. The significance level was fixed at 0.05, and all Even if without altering the shape of the defini- The color measurement has been obtained tests were two tailed. All the analyses were tive prosthesis from the final one, no pressure on using a spectrophotometer (Spectroshade ‘‘Mi- performed using the SAS statistical software rel. the soft tissue was noticed, 10 min were left cro’’ Device, MHT S.p.A., Medical High Tech- 9.1 (SAS Institute, Cary, NC, USA). 2010 John Wiley Sons A/S c 3 | Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x
  • 4. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations Table 2. Estimated least square mean Æ stan- dard errors (SE) and 95% confidence intervals (95% CI) of DE by abutment materials Mean Æ SE 95% CI Gold 8.9a Æ 0.4 8.1–9.7 Titanium 11b Æ 0.4 10.2–11.9 Zirconium 8.5a Æ 0.4 7.6–9.3 Different superscript letters indicate significantly (Po0.05) different mean values (Scheffe’s test for multiple comparison). naked eye. When the crown was placed over the abutment, the DE mean value between the peri- implant soft tissue and the contra-lateral gingival tissue was similar for gold and zirconium abut- ment (8.9 and 8.5, respectively), but it was sig- nificantly greater with the titanium abutment (11). Fig. 4. Gold-alloy abutment screwed into position. The calculated Lab (L, a, b) values of the measured areas around abutments and natural teeth were summarized on Table 3. For each feature, natural teeth mean values were signifi- cantly higher than those of the different types of abutment. In addition, gold and titanium abut- ments provided no significantly different mean values both for L and b color measurement, while titanium abutment always provided lower mean values. For the a color measurements, no differ- ences were evident among the three types of abutment. Gold, titanium and zirconia abutment measurements were significantly different (Po0.001) from contra-lateral teeth (control sites) measurements. Calculated Lab values of different abutments with correspondent 95% CI are reported in Fig. 7; control sites were considered as reference. All the Fig. 5. Zirconia abutment screwed into position. differences evaluated by using paired data were statistically significant, as shown by 95% CIs (zero value not included). Fig. 7 also showed that titanium abutment exhibited worse color perfor- mances than gold and zirconium abutments. The zirconia results were likely to achieve the best color matching with natural teeth, but the differ- ences with gold performance were never statisti- cally significant. To verify the role of soft tissue thickness on the color measurements with the different abutment types, a stratified analysis was performed by dichotomizing the thickness on 2 mm (thin 2 mm; thick 42 mm). Seven patients were included in the ‘‘Thin’’ group and the other 13 patients made up the ‘‘Thick’’ group, respec- tively. The DE mean value did not depend on the soft tissue thickness (Table 4), and thickness ¨ might only relate to the Ab color aspect (Table 5). Fig. 6. Selected area identified through spectrophotometer software: 2 mm in thickness (red arrow) and 4 mm in apico-coronal direction (green arrow). Results matter which type of restorative material was Discussion selected (Table 2). Measurements relative to all The peri-implant soft tissue color was different the abutments were above the critical threshold The present study evaluated the color-change from the soft tissue color around natural teeth, no of DE 3.7 for intraoral color distinction by the effect on marginal peri-implant soft tissue of all- 4 | Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x 2010 John Wiley Sons A/S c
  • 5. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations Table 3. Estimated least square mean Æ standard errors (SE) of Lab values: measured areas around Table 5. Correlation coefficients between soft abutments and natural teeth by abutment materials tissue thickness (mm) and the difference for the Lab values of the measured areas around abut- Gold Titanium Zirconium NT ments and natural teeth or DE, by abutment Mean Æ SE Mean Æ SE Mean Æ SE Mean Æ SE materials Gold Titanium Zirconium Ln 47.1a,b Æ 0.6 45.6a Æ 0.6 48.1b Æ 0.6 52.9c Æ 0.6 an 22.1a Æ 0.5 21.5a Æ 0.5 22.9a Æ 0.5 25.5b Æ 0.5 DL 0.2 (0.38) À 0.06 (0.81) À 0.04 (0.87) bn 14.9a,b Æ 0.3 13.9a Æ 0.3 15.4b Æ 0.3 18.4c Æ 0.3 DA À 0.3 (0.19) À 0.26 (0.26) À 0.2 (0.38) DB À 0.45 (0.05) À 0.4 (0.08) À 0.4 (0.08) Different superscript letters indicate significantly different mean values. DE 0.08 (0.74) 0.09 (0.71) 0.13 (0.6) Significance in brackets. study showed a significant difference between the color of the peri-implant soft tissue and the color of the gingival around natural teeth no matter which type of prosthesis is used. As described on Table 2, DEs were always higher than the critical threshold of 3.7 (Johnston Kao 1989). This value, described as higher value for color matching between natural teeth and re- stored teeth, might have only a relative impact on the analysis of the soft tissue but represent the most reported reference in the literature (Ishi- kawa et al. 1988; Jung et al. 2008; Lops et al. 2008; Romeo et al. 2008). Other values reported in the tooth color matching literature are even lower (Douglas, 1997; Douglas Brewer, 1998). The absence in the literature of a reference for soft tissue color matching may represented a limitation in the analysis of present results, but the DE values obtained are significantly higher than the reported color differences between the soft tissues of contra-lateral natural teeth, which were within 2.7 (Zembic et al. 2009). As described on Table 3, significantly different mean values have been found between titanium on one side and gold and zirconia on the other side. While no differences were present in the color performance of gold and zirconium oxide abtments, the color of the soft tissue around titanium abutment was significantly more differ- ent from the gingival color around natural teeth. Fig. 7. Calculated Lab values with correspondent 95% confidence interval represented by type of abutment: taking not In addition, all the single values (Ln, an, bn) treated teeth (control site) taken as reference. were different from the values obtained around natural teeth. Among the different type of abut- ments, no significant differences were present in Table 4. DE mean values and standard errors (SE) by soft tissue thickness and abutment materials considering the red and green scale of a values, Soft tissue thickness (mean Æ SE) P confirming a trend already reported in the litera- 2 mm (7 pt) 42mm (13 pt) ture (Hermann et al. 2001). In the analysis of Ln Gold 8.6 Æ 1.4 9.1 Æ 0.8 0.74 and bn values, significantly differences were pre- Titanium 9.5 Æ 1.4 11.9 Æ 1.2 0.25 sent among the abutment. The peri-implant soft Zirconium 7.5 Æ 1.4 8.9 Æ 0.7 0.38 tissue around zirconium oxide abutments ap- peared to be significantly closer to the color of natural teeth gingival than titanium and gold ceramic restorations based on zirconium oxide, over, different from previous studies, only one abutment provided interposed results. Also, gold-alloy and titanium abutments. The pecu- type of crown was utilized, relating the results (Jung et al. 2008) noticed clinically gingival color liarity of this trial was that measurements were only to the abutment variable. differences between around peri-implant soft tis- performed for all three abutment materials in In accordance with the data available in the sue in comparison with the contra-lateral tooth. each patient; therefore, important patient-related international literature by (Sailer et al. 2009) and Nevertheless, their results suggested that all- and material-related biases were avoided. More- (Zembic et al. 2009), the results of the present ceramic restorations on aluminum oxide abut- 2010 John Wiley Sons A/S c 5 | Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x
  • 6. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations ments provide a significantly better color match at 2 mm: 7 patients with thin and 13 with thick esting to compare the accuracy of this method to the un-restored neighboring teeth than porce- peri-implant soft-tissues were observed. As with that used by (Sailer et al. 2009) and (Zembic lain-fused-to-metal restorations on titanium or shown on Table 4, no statistically significant et al. 2009). They recorded the soft tissue thick- gold abutments. Unfortunately, no specification differences were present in the color of the ness in the region of esthetic assessment both at on the number of gold or titanium abutment nor mucosa between thick and thin tissue. Even if a implants and teeth using an endodontic file (ISO a description on the possible different outcome trend of higher values of differences was noticed, #20) with a rubber stop. present between these two types of abutment has especially with titanium abutment, in the thick been made. Moreover, considering the properties group, this study did not confirm that mucosa of zirconium oxide vs. aluminum oxide, zirco- thickness mucosa is a crucial factor in terms of nium oxide should be referred on implant- discoloration as concluded by (Jung et al. 2007). supported restorations (Butz et al. 2005; Canullo, Most probably this result differed from the pre- Conclusions 2007; Garine et al. 2007). vious literature due to the reduced sample size. Another in vitro study by (Jung et al. 2007) Another reason for this trend could be that no soft Within the limitation of the resent study, the reported a decrease of the color change related to tissue thickness ! 3 mm was found in the following conclusions can be drawn: an increasing of the mucosa thickness over zirco- group classified as ‘‘Thick.’’ Further researches nia and titanium. In situations with a mucosal on more representative patient samples are re- the peri-implant soft tissue color is different thickness of 1.5 mm, both materials demon- quested to confirm or refuse this trend. from the soft tissue color around natural strated DE values above the critical threshold of Regarding an eventual shortcoming of the thick- teeth, no matter which type of restorative 3.7 (Park et al. 2007), with a score of 3.87 for ness measurement by means of a caliper applied to material is selected. zirconium oxide and 5.06 for titanium. With a the study cast), the authors considered that a titanium abutments are associated with sig- mucosa thickness of 2 mm, the color change customized pick-up was able to permit a precise nificantly higher differences than those ob- induced by zirconia was below the threshold of reproduction of the trans-mucosal soft tissue cone, tained with gold or zirconia abutments. 3.7 (3.17), whereas titanium still caused a visible and a light-consistence polyether material could no significant differences are present between difference (4.32). With a 3 mm thickness, all the not interfere in the soft tissue thickness re-produc- the color of peri-implant soft tissue around abutment scored within the limit of 3.7 (titanium tion. Probably, the subsequent step of the gingival zirconia abutment and the one around gold 2.14 and zirconia 2.47). re-construction on the master cast by means of a abutment. For this reason, the present study also tried to siliconic material could add some degree of inac- the thickness of the peri-implant soft tissue assess possible in vivo implication of the thick- curacy (maybe fraction of millimeter) in the peri- does not appear to be a crucial factor in the ness of peri-implant mucosa by dichotomizing it implant mucosa re-production. It could be inter- abutment impact on the soft tissue color. References Berns, R. (2000) Principle of Color T echnology. 3rd Gallucci, G.O., Belser, U.C., Bernard, J. Magne, P. Heydeck, E.G., Sierralta, M. Razzoog, M.E. (2002) edition. New York: Wiley. (2004) Modeling and characterization of the CEJ for Evolution and use of aluminum oxide single-tooth Bichacho, N. Landsberg, C.J. (1997) Single implant optimizing of Esthetic implant design. 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  • 7. Bressan et al Á Influence of abutment material on the gingival color of implant-supported restorations Lops, D., Chiapasco, M., Rossi, A., Bressan, E. Romeo, E., Lops, D., Rossi, A., Storelli, S., Rozza, R. .Konzept zur a ¨sthetischen Verbesserung der Supras- Romeo, E. (2008) Incidence of inter-proximal papilla Chiapasco, M. (2008) Surgical and prosthetic manage- truktur. Implantologie: Quintessenz Zahntechnik between a tooth and an adjacent immediate implant ment of interproximal region with single-implant 22: 364–381. placed into a fresh extraction socket: 1-year prospec- restorations: 1-year prospective study. Journal of Zembic, A., Sailer, I., Jung, R.E. Ha ¨mmerle C, H.F. tive study. Clinical Oral Implant Research 19: Periodontology 79: 1048–55. (2009) Randomized-controlled clinical trial of custo- 1135–40. Rompen, E., Raepsaet, N., Domken, O., Touati, B. mized zirconia and titanium implant abutments for Munsell, A.H. (1923) A Color Notation. Baltimore, Van Dooren, E. (2007) Soft tissue stability at the facial single tooth implants in canine and posterior regions: MD: Munsell Color Company. aspect of gingivally converging abutments in the 3-year results. Clinical Oral Implant Research 20: Nakamura, T., Saito, O., Fuyikawa, J. Ishigaki, S. esthetic zone: a pilot clinical study. Journal of Pros- 802–808. (2002) Influence of abutment substrate and ceramic thetic Dentistry 97: 119–125. thickness on the color of heat-pressed ceramic Sailer, I., Zembic, A., Jung, R.E., Siegenthaler, D., crowns. Journal of Oral Rehabilitation 29: Holderegger, C. Ha ¨mmerle, C.H.F. (2009) Rando- Supporting Information 805–809. mized controlled clinical trial of customized zirconia Park, S.E., Da Silva, J.D., Weber, H.P. Ishikawa- and titanium implant abutments for canine and Additional Supporting Information may be found Nagai, N. (2007) Optical phenomenon of peri- posterior single-tooth implant reconstructions: preli- in the online version of this article: implant soft tissue. Part I. Spectrophotometric minary results at 1 year of function. Clinical Oral assessment of natural tooth gingival and peri-implant Implant Research 20: 219–225. mucosa. Clinical Oral Implant Research 18: Sykaras, N., Iacopino, A.M, Marker, V.A., Triplett, R.G Table S1. Supporting information in accordance 569–574. Woody, R.D. (2000) Implant materials, designs, with the CONSORT Statement 2001 checklist Prestipino, V. Ingber, A. (1993a) Esthetic high and surfaces topographies: their effect on osseointe- used in reporting randomized trials. strength implant abutments. Part I. Journal of gration. A literature review. The International Jour- Esthetic Dentistry 5: 29–36. nal of Oral Maxillofacial Implants 15: 675–690. Please note: Wiley-Blackwell is not responsible Quirynen, M., Van Assche, N., Botticelli, D. Ber- Tarnow, D.P. Eskow, R.N. (1996) Preservation of for the content or functionality of any supporting glundh, T. (2007) How does the timing of implant implant esthetics. Soft tissue and restorative consid- placement to extraction affect the outcome? The erations. Journal of Esthetic Dentistry 8: 12–19. materials supplied by the authors. Any queries International Journal of Oral Maxillofacial Im- Wohlwend, A., Studer, S. Scha ¨rer, P. (1996) Das (other than missing material) should be directed plants 22: 203–223. Zirkonoxidabutment-Ein neues vollkera-misches to the corresponding author for the article. 2010 John Wiley Sons A/S c 7 | Clin. Oral Impl. Res. 10.1111/j.1600-0501.2010.02008.x