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The diamondback moth, Plutella xylostella, is one of the most important pests of cruciferous crops in the world. It spreads worldwide, causing huge financial losses. Insects became resistant to currently most of chemical pesticides. Actually, populations of Plutella xylostella are almost impossible to control. However, there is a specific virus to the larvae of this species, the PxGV, which is able to significantly reduce their impact, while being environmentally friendly. NPP decided to set up a breeding of this insect on semi-synthetic medium to study the biotic parameters of the species in order to produce the virus on an industrial scale.