Aflatoxin Test kits
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Rachel W. Lilies, Sales Coordinator, Food Safety, F&S Scientific
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Aflatoxin Test kits
- 1. Rachel W. Lilies Sales Coordinator, Food Safety
- 2. Agenda F&S Introduction Aflatoxin Test kits Effectiveness Pricing Partnership
- 3. Objectives 1. To learn about the available Aflatoxin test kits from F& S 2. To understand the effectiveness levels in terms of adoption and uptake for small holder farmers 3. To know the Market price for the testing kits. 4. To understand our future Partnership with project partners.
- 4. Introducing F&S Scientific The one stop solution for all your laboratory requirements Brief History With over 25 years experience of providing laboratory equipment and consumables in East and Central Africa Product Sectors Analytical Chemistry Life Science Clinical Diagnostics Industries - Food & Beverage - Research - Agriculture & Livestock - Environmental - Healthcare - Pharmaceutical & Cosmetics Our is mission is to assist our customers in making Africa Cleaner, Safer and Healthier
- 5. Our products FOOD SAFETY FOOD QUALITY - Mycotoxin Testing - Microbial Testing - Allergen testing - Proximate Analysis - Moisture Analysis - Fortification Analysis - Colour Testing
- 6. Our Brands
- 7. OUR CUSTOMERS FOOD & DAIRY INDUSTRY MILLERS INDEPENDENT LABS/STATUTORY /RESEARCH ORGAIZATIONS FEEDS INDUSTRY
- 8. “Farm to fork” Supply Chain FARMING Storage Farming HANDLING Storage Transport PROCESSING Milling Storage RETAILING Shelfing Distribution CONSUMER Cleanliness Temperature Humidity Sorting Ventilation Processing practices Disposal Temperature Humidity Hygiene Cooking Storing
- 9. “Farm to fork” Supply Chain FARMING Storage Farming HANDLING Storage Transport PROCESSING Milling Storage RETAILING Shelfing Distribution CONSUMER Cleanliness Temperature Humidity Sorting Ventilation Processing practices Disposal Temperature Humidity Hygiene Cooking Storing WHERE THINGS CAN GO WRONG… Aflasafe AflaStop APTECA APTECA APTECA
- 10. Don’t count on these to test for food safety! SIGHT SMELL TASTE TESTING IS THE ONLY WAY TO TRULY ASSESS CONTAMINATION OF AFLATOXINS
- 11. Agenda F&S Introduction Aflatoxin Test kits Effectiveness Pricing Partnership
- 13. Next generation reader for Neogen® ’s lateral flow diagnostics Easy to operate Field Application Consistency Simple PC Interface
- 14. Lateral Flow • Lateral Flow Immunochromatographic is a rapid assay in the test strip format. • The test involves the binding of antigens (i.e. Aflatoxin) FEATURE BENEFIT • Simple protocol Easy to use • Aflatoxin detection range : 2 – 150 ppb Low and high range detection ability • Very short method Rapid tests (results in 6 minutes) • Single sample analysis Ideal for low throughput • Room temperature storage Reduces storage costs & time to equilibrate kits to room temp. • Room temperature incubation Lower initial cost to set up, no incubator • Ethanol/Water extraction Simple & safe • AccuScan Gold Reader Unique, easy-to-use colour touch screen reader. • AccuScan Gold Data Manger software Traceability & data storage • USDA/GIPSA Approved
- 15. Analysis of Aflatoxin Using Reveal Q+ Kits
- 16. Analysis of Aflatoxin Using Reveal Q+ Kits…contd.
- 17. Stat Fax 4700 Microwell Reader- ELISA Touch Screen Technology Automatic strip transportation Lab Application
- 18. ELISA • Uses Antibodies/Antigen binding & Colour Change to measure the concentration of a substance FEATURE BENEFIT • Simple protocol Relatively easy to use • Aflatoxin detection range = 1 – 50 ppb Low and high range detection ability • Short method Fairly rapid tests (results in 15 minutes) • Batch testing Ideal for running several tests at the same time • Refrigerated storage • Room temperature incubation Lower initial cost to set up, no incubator • Methanol/Water extraction Simple & safe • Stat-Fax Reader Easy-to-use colour touch screen reader. • Veratox Data Manger software Traceability & data storage
- 19. Analysis of Aflatoxin Using Veratox Kits 1. Obtain a representative sample . Grind and Weigh 10g Add 50ml of 70% Methanol AR to sample
- 20. Analysis of Aflatoxin Using Veratox Kits….cont.
- 21. Comparison Lateral Flow ELISA Quantitative • Test strips • Microwell tests Results in • 6 minutes to results • 15 minutes to results Detection Range (Afla) • 2-150 ppb • 5-50 ppb Incubation • Room temperature • Room temperature Storage • Room temperature • Refrigeration: 2-8°C Throughput • Single sample • Batch samples Field & Lab use Application • Field & Lab • Lab only Skill requirement • Low • Moderate
- 22. Agenda F&S Introduction Aflatoxin Test kits Effectiveness Pricing Partnership
- 23. Price Item KES(excl. VAT) USD COST PER TEST Reveal Q+ for Aflatoxin - 25strips 23,000 230 920 Accuscan Gold Complete Solution 450,000 4,500 Veratox for Aflatoxin- 40 Microwells 25,000 250 625 Stat Fax 4700 Reader 550,000 5,500
- 24. Agenda F&S Introduction Aflatoxin Test kits Effectiveness Pricing Partnership
- 25. Active Projects APTECA: Aflatoxin Proficiency Testing & Control in East & Central Africa Emphasizes Aflatoxin testing Accuracy thro”: Uniform sampling Testing equipment & methods Proficiency testing Use of Lab controls samples 3rd party verification by Texas A&M AgriLife Research at the BecA-ILRI Hub
- 26. THANK YOU FOR LISTENING… QUESTION TIME… ASK AWAY
- 27. Rachel W. Lilies Mobile No: +254 725114 957 Email Address: rachel.wangui@fnscientific.com Switch Board: +254 203594777 Shamneel Court ,2Muthithi Road, Westlands P.O Box 39081-00623 Nairobi, Kenya www.fnscientific.com
Notes de l'éditeur
- Single-step lateral flow assays based on a competitive immunoassay format. The extract is wicked through a reagent zone, which contains antibodies specific for the target Mycotoxin conjugated to colloidal gold particles. If the target mycotoxin is present, it will be captured by the particle-antibody complex. The mycotoxin-labeled antibody complex is then wicked onto a membrane, which contains a zone of mycotoxin. This zone captures any unbound mycotoxin antibody, allowing the particles to concentrate and form a visible line. As the level of mycotoxin in a sample increases, free mycotoxin will bind with the antibody-gold particles. This allows less antibody-gold to be captured in the test zone. Therefore, as the concentration of target mycotoxin in the sample increases, the test line density decreases. The membrane also contains a control line which will always form regardless of the presence of mycotoxin, ensuring the strip is functioning properly.
- Voluntary process verification for Aflatoxin risk management for grain handlers & millers. Accurate measurement challenging: sampling & testing Emphasizes Aflatoxin testing Accuracy thro”: Uniform sampling Testing equipment & methods Proficiency testing Use of Lab controls samples 3rd party verification by Texas A&M AgriLife Research at the BecA-ILRI Hub