1. Characteristics of the
Pre-Breeding Parent Lines
Developed in Peach
green peach aphid, powdery mildew,
brown rot, non-acid-flesh, blood-flesh, …
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2. Develop advanced material with new traits for peach breeders
¿Which new traits are targeted to INRA-Avignon?
Traits of resistance to:
- green peach aphid
- peach powdery mildew
- Plum Pox Virus (M strain)
- brown rot (Monilinia laxa)
- nematodes
Traits of “fruit quality” as:
- blood-flesh (DBF and bf)
- high-content in 2ary metabolites
Traits of the tree habit as:
- weeping shape
- semi-double flower
so far little attention …

3. Develop advanced material with new traits for peach breeders
Characteristics of resistance sources ... qualitative/quantitative
green peach powdery
aphid
mildew
plum pox
virus
brown
rot
Weeping Flower Peach
Rm1
-
-
-
Rubira
Rm2
-
-
-
Malo Konare
-
Vr1
-
-
Pamirskij 5
-
Vr2
-
-
BC2 from P. davidiana
-
-
-
ongoing (WP5)
Bolinha
-
-
-
ongoing (WP5)
P. davidiana
MP.SD-3.1
MP.SD-5.1
PM.SD-6.1
PM.SD-8.1
-
P. dulcis
ongoing
ongoing
PPV.RD-1.1
PPV.RD-2.1
PPV.RD-7.1
ongoing
?

4. Develop advanced material with new traits for peach breeders
Characteristics of resistance sources ... first by classical phenotyping
green peach
aphid
powdery
mildew
plum pox
virus
Weeping Flower Peach
Rubira
Malo Konare
Pamirskij 5
BC2 from P. davidiana
TIC
(Test with
Inoculation
on Cultivar)
Bolinha
P. davidiana
P. dulcis
TIR
(Test with
Inoculation
on Rootstock)
brown
rot

5. Develop advanced material with new traits for peach breeders
Characteristics of resistance sources ... then mapped on genome
GL1
GL2
PPV.RD-1.1
resistance to
PPV
Y/y
white/yellow
flesh color
PPV.RD-2.1
resistance to
PPV
RMia
resistance to
nematodes
bf
blood-flesh
color
pl
weeping habit
SD-MP.3.1
resistance to
green peach
aphid
GL6
GL5
GL4
GL3
GL7
GL8
Vr2
resistance to
peach powdery
mildew
DBF
blood-flesh color
D
non-acid flesh
SD-MP.3.1
resistance to
green peach
aphid
SD
semi-double
flower
SD-PM.6.1
resistance to
powdery mildew
PPV.RD-7.1
resistance to
PPV
SD-PM.8.1
resistance to
powdery
mildew
g
glabrous skin
S*
flat fruit
Rm2
resistance to
green peach
aphid
Peach genetic linkage map (8 chromosomes)
SSR, SNP-KASP® molecular markers available for MAB for most of these traits

6. Develop advanced material with new traits for peach breeders
Characteristics of the peach species ... growing cycle very short
- March 2014: crosses
- June to September: harvest and statification in cold chamber (or 6 BAP)
- Winter 2014/2015 : early selection screening under greenhouse conditions
or by Marker molecular Assisted Selection …
- Springtime & Summer 2015: growing of resistant genotypes selected previously
- March 2016: new crosses with flowers coming from resistants genotypes …
according objectives, one generation all two years is easily feasible in peach

7. Develop advanced material with new traits for peach breeders
Characteristics of the peach species ... growing cycle very short
example with P. davidiana and the resistance to pow. mildew
x
P. davidiana
Summergrand
sssssssSummergrand
1st generation
2nd generation
“fast breeding” in peach (3 generations / 6 years)
with a rapid progress on fruit traits …
3rd generation

8. Develop advanced material with new traits for peach breeders
Characteristics of the pre-breeding parent lines …
- di-allele crosses between resistant genitors and recurrent parents as Summergrand …
100 to 200 individuals by combination stored in conservatory
-flat fruit or blood-flesh (DBF) pre-selections
bearing 1 / 2 single resist. genes (Rm1 / Vr1)
- crosses realized to associated different resistance factors to green peach aphid powdery
mildew … in the aim to improve the durability of these resistances